This is a Validated Antibody Database (VAD) review about human F11R, based on 25 published articles (read how Labome selects the articles), using F11R antibody in all methods. It is aimed to help Labome visitors find the most suited F11R antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
F11R synonym: CD321; JAM; JAM1; JAMA; JCAM; KAT; PAM-1

Knockout validation
Abcam
domestic rabbit monoclonal (EP1042Y)
  • western blot knockout validation; human; 1:2000; loading ...; fig 2b
  • immunohistochemistry - paraffin section; human; 1:100; fig 1a
Abcam F11R antibody (Abcam, ab52647) was used in western blot knockout validation on human samples at 1:2000 (fig 2b) and in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1a). Cancer Sci (2021) ncbi
Invitrogen
mouse monoclonal (CSTEM27)
  • immunocytochemistry; human; 1:100; loading ...; fig 6a
Invitrogen F11R antibody (Thermo Fisher, 14-3219-82) was used in immunocytochemistry on human samples at 1:100 (fig 6a). Front Immunol (2020) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:250; loading ...; fig 3a
In order to explore how laminin2 in the basement membrane modulates the blood testis barrier dynamics during spermatogenesis, Invitrogen F11R antibody (Thermo Fisher Scientific, 36-1700) was used in western blot on rat samples at 1:250 (fig 3a). FASEB J (2017) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:250; loading ...; tbl 1
In order to examine the organization of actin microfilaments in Sertoli cell at the blood testis barrier, Invitrogen F11R antibody (Invitrogen, 36-1700) was used in western blot on rat samples at 1:250 (tbl 1). Spermatogenesis (2016) ncbi
domestic rabbit polyclonal
  • western blot; dogs; 1:1000; fig 3a
In order to investigate how serine proteases increase transepithelial electrical resistance, Invitrogen F11R antibody (Invitrogen, 36-1700) was used in western blot on dogs samples at 1:1000 (fig 3a). Am J Physiol Gastrointest Liver Physiol (2016) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:250; fig 5
In order to report that crumbs homolog 3 is an actin microfilament regulator, Invitrogen F11R antibody (Invitrogen, Life Technologies, 36-1700) was used in western blot on rat samples at 1:250 (fig 5). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:150; fig 3
In order to determine regulation of ectoplasmic specialization dynamics via its effects on actin microfilaments in the testes of male rats by planar cell polarity (PCP) protein Vangl2, Invitrogen F11R antibody (Invitrogen, 361700) was used in western blot on rat samples at 1:150 (fig 3). Endocrinology (2016) ncbi
domestic rabbit polyclonal
  • proximity ligation assay; human; loading ...; fig 6b
  • immunocytochemistry; human; loading ...; fig 6b
In order to explore how junctional adhesion molecule family members differentially regulate CXCR4 function and CXCL12 secretion in the bone marrow niche, Invitrogen F11R antibody (Invitrogen, 36-1700) was used in proximity ligation assay on human samples (fig 6b) and in immunocytochemistry on human samples (fig 6b). Stem Cells (2016) ncbi
domestic rabbit polyclonal
In order to analyze infertility in mice by deletion of the tyrosine phosphatase Shp2 in Sertoli cells, Invitrogen F11R antibody (Invitrogen, 36?C1700) was used . Sci Rep (2015) ncbi
domestic rabbit polyclonal
In order to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis, Invitrogen F11R antibody (Invitrogen, 36-1700) was used . FASEB J (2015) ncbi
domestic rabbit polyclonal
In order to investigate the effect of homoharringtonine on intestinal epithelial permeability and its mechanism, Invitrogen F11R antibody (Invitrogen, 36-1700) was used . Eur J Pharm Biopharm (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (1H2A9)
  • immunocytochemistry; human; 1:50; loading ...; fig s7c
Santa Cruz Biotechnology F11R antibody (Santa Cruz Biotechnology, sc-53624) was used in immunocytochemistry on human samples at 1:50 (fig s7c). Proc Natl Acad Sci U S A (2021) ncbi
mouse monoclonal (J10.4)
  • flow cytometry; Orthoreovirus; fig 2d
Santa Cruz Biotechnology F11R antibody (Santa Cruz, 53623) was used in flow cytometry on Orthoreovirus samples (fig 2d). Sci Rep (2017) ncbi
mouse monoclonal (1H2A9)
  • immunocytochemistry; human; fig s1
Santa Cruz Biotechnology F11R antibody (santa Cruz, sc-53624) was used in immunocytochemistry on human samples (fig s1). Mol Biol Cell (2015) ncbi
mouse monoclonal (J10.4)
  • western blot; human; 1:500
Santa Cruz Biotechnology F11R antibody (Santa Cruz, sc-53623) was used in western blot on human samples at 1:500. J Immunol (2015) ncbi
mouse monoclonal (1H2A9)
  • western blot; human
Santa Cruz Biotechnology F11R antibody (Santa Cruz Biotechnology, 1H2A9) was used in western blot on human samples . Stem Cells (2014) ncbi
Abcam
domestic rabbit monoclonal (EP1042Y)
  • western blot knockout validation; human; 1:2000; loading ...; fig 2b
  • immunohistochemistry - paraffin section; human; 1:100; fig 1a
Abcam F11R antibody (Abcam, ab52647) was used in western blot knockout validation on human samples at 1:2000 (fig 2b) and in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1a). Cancer Sci (2021) ncbi
domestic rabbit monoclonal (EP1042Y)
  • western blot; mouse; loading ...; fig 5k
In order to determine the effects of an altered intestinal microbiota and microbial metabolites on graft-versus-host disease, Abcam F11R antibody (abcam, EP1042Y) was used in western blot on mouse samples (fig 5k). Nat Immunol (2016) ncbi
domestic rabbit monoclonal (EP1042Y)
  • immunohistochemistry; human; 1:100; loading ...; fig 12
Abcam F11R antibody (Abcam, ab52647) was used in immunohistochemistry on human samples at 1:100 (fig 12). Int J Oncol (2015) ncbi
BioLegend
mouse monoclonal (OV-5B8)
  • flow cytometry; human; loading ...; tbl s1
In order to explore how junctional adhesion molecule family members differentially regulate CXCR4 function and CXCL12 secretion in the bone marrow niche, BioLegend F11R antibody (Biolegend, 353504) was used in flow cytometry on human samples (tbl s1). Stem Cells (2016) ncbi
mouse monoclonal (OV-5B8)
  • flow cytometry; human; fig 5
In order to study dual involvement of S1P1 and S1P2 receptors in regulation of human cerebro-microvascular endothelial baso-lateral adhesion and barrier function by S1P, BioLegend F11R antibody (BioLegend, 353503) was used in flow cytometry on human samples (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (OV-5B8)
  • flow cytometry; human; fig 5
In order to determine the differential regulation of the inflammatory phenotype of brain microvascular endothelial cells by pro-inflammatory TNFalpha and IL-1beta, BioLegend F11R antibody (Biolegend, 353503) was used in flow cytometry on human samples (fig 5). J Neuroinflammation (2015) ncbi
Abnova
mouse monoclonal (2E3-1C8)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1a, 1c
  • western blot; human; 1:500; loading ...; fig 3a
Abnova F11R antibody (Abnova, H00050848-M01) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1a, 1c) and in western blot on human samples at 1:500 (fig 3a). J Surg Res (2014) ncbi
BD Biosciences
mouse monoclonal (43/JAM-1)
  • western blot; human; fig 5
BD Biosciences F11R antibody (BD Transduction, 612120) was used in western blot on human samples (fig 5). Mol Biol Cell (2016) ncbi
mouse monoclonal (M.Ab.F11)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences F11R antibody (BD, 552556) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (M.Ab.F11)
  • flow cytometry; human; loading ...; tbl s1
In order to explore how junctional adhesion molecule family members differentially regulate CXCR4 function and CXCL12 secretion in the bone marrow niche, BD Biosciences F11R antibody (BD Pharmingen, BD552556) was used in flow cytometry on human samples (tbl s1). Stem Cells (2016) ncbi
mouse monoclonal (43/JAM-1)
  • immunoprecipitation; human; loading ...; fig 5a
  • western blot; human; loading ...; fig 7a
In order to explore how junctional adhesion molecule family members differentially regulate CXCR4 function and CXCL12 secretion in the bone marrow niche, BD Biosciences F11R antibody (BD Biosciences, 43/JAM-1) was used in immunoprecipitation on human samples (fig 5a) and in western blot on human samples (fig 7a). Stem Cells (2016) ncbi
mouse monoclonal (43/JAM-1)
  • western blot; human; fig s6
BD Biosciences F11R antibody (BD Biosciences, 612120) was used in western blot on human samples (fig s6). Mol Biol Cell (2015) ncbi
mouse monoclonal (M.Ab.F11)
  • flow cytometry; human; fig 6
BD Biosciences F11R antibody (BD Biosciences, M. Ab F11) was used in flow cytometry on human samples (fig 6). J Leukoc Biol (2015) ncbi
MilliporeSigma
mouse monoclonal (J10.4)
  • western blot; human; 1:1000; fig 1a
MilliporeSigma F11R antibody (Sigma-Aldrich, SAB4200468) was used in western blot on human samples at 1:1000 (fig 1a). PLoS Pathog (2019) ncbi
Articles Reviewed
  1. Wijshake T, Zou Z, Chen B, Zhong L, Xiao G, Xie Y, et al. Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  2. Murakami T, Takasawa A, Takasawa K, Akimoto T, Aoyama T, Magara K, et al. Aberrant expression of junctional adhesion molecule-A contributes to the malignancy of cervical adenocarcinoma by interaction with poliovirus receptor/CD155. Cancer Sci. 2021;112:906-917 pubmed publisher
  3. Matsubara J, Tian Y, Cui J, Zeglinski M, Hiroyasu S, Turner C, et al. Retinal Distribution and Extracellular Activity of Granzyme B: A Serine Protease That Degrades Retinal Pigment Epithelial Tight Junctions and Extracellular Matrix Proteins. Front Immunol. 2020;11:574 pubmed publisher
  4. Takeuchi H, Sasaki N, Yamaga S, Kuboniwa M, Matsusaki M, Amano A. Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1. PLoS Pathog. 2019;15:e1008124 pubmed publisher
  5. Dautzenberg I, Van Den Hengel S, de Vrij J, Ravesloot L, Cramer S, Hong S, et al. Baculovirus-assisted Reovirus Infection in Monolayer and Spheroid Cultures of Glioma cells. Sci Rep. 2017;7:17654 pubmed publisher
  6. Gao Y, Mruk D, Chen H, Lui W, Lee W, Cheng C. Regulation of the blood-testis barrier by a local axis in the testis: role of laminin ?2 in the basement membrane. FASEB J. 2017;31:584-597 pubmed publisher
  7. Li N, Lee W, Cheng C. Overexpression of plastin 3 in Sertoli cells disrupts actin microfilament bundle homeostasis and perturbs the tight junction barrier. Spermatogenesis. 2016;6:e1206353 pubmed publisher
  8. Ronaghan N, Shang J, Iablokov V, Zaheer R, Colarusso P, Dion S, et al. The serine protease-mediated increase in intestinal epithelial barrier function is dependent on occludin and requires an intact tight junction. Am J Physiol Gastrointest Liver Physiol. 2016;311:G466-79 pubmed publisher
  9. Gao Y, Lui W, Lee W, Cheng C. Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells. Sci Rep. 2016;6:28589 pubmed publisher
  10. Mathewson N, Jenq R, Mathew A, Koenigsknecht M, Hanash A, Toubai T, et al. Gut microbiome-derived metabolites modulate intestinal epithelial cell damage and mitigate graft-versus-host disease. Nat Immunol. 2016;17:505-513 pubmed publisher
  11. Chen H, Mruk D, Lee W, Cheng C. Planar Cell Polarity (PCP) Protein Vangl2 Regulates Ectoplasmic Specialization Dynamics via Its Effects on Actin Microfilaments in the Testes of Male Rats. Endocrinology. 2016;157:2140-59 pubmed publisher
  12. Scott D, Tolbert C, Burridge K. Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF. Mol Biol Cell. 2016;27:1420-30 pubmed publisher
  13. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  14. Chang C, Hale S, Cox C, Blair A, Kronsteiner B, Grabowska R, et al. Junctional Adhesion Molecule-A Is Highly Expressed on Human Hematopoietic Repopulating Cells and Associates with the Key Hematopoietic Chemokine Receptor CXCR4. Stem Cells. 2016;34:1664-78 pubmed publisher
  15. Wiltshire R, Nelson V, Kho D, Angel C, O Carroll S, Graham E. Regulation of human cerebro-microvascular endothelial baso-lateral adhesion and barrier function by S1P through dual involvement of S1P1 and S1P2 receptors. Sci Rep. 2016;6:19814 pubmed publisher
  16. Hu X, Tang Z, Li Y, Liu W, Zhang S, Wang B, et al. Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice. Sci Rep. 2015;5:12982 pubmed publisher
  17. Scott D, Tolbert C, Graham D, Wittchen E, Bear J, Burridge K. N-glycosylation controls the function of junctional adhesion molecule-A. Mol Biol Cell. 2015;26:3205-14 pubmed publisher
  18. O Carroll S, Kho D, Wiltshire R, Nelson V, Rotimi O, Johnson R, et al. Pro-inflammatory TNFα and IL-1β differentially regulate the inflammatory phenotype of brain microvascular endothelial cells. J Neuroinflammation. 2015;12:131 pubmed publisher
  19. Li N, Mruk D, Wong C, Lee W, Han D, Cheng C. Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis. FASEB J. 2015;29:3788-805 pubmed publisher
  20. Carvallo L, Lopez L, Che F, Lim J, Eugenin E, Williams D, et al. Buprenorphine decreases the CCL2-mediated chemotactic response of monocytes. J Immunol. 2015;194:3246-58 pubmed publisher
  21. Khajah M, Mathew P, Alam Eldin N, Luqmani Y. Bleb formation is induced by alkaline but not acidic pH in estrogen receptor silenced breast cancer cells. Int J Oncol. 2015;46:1685-98 pubmed publisher
  22. Watari A, Hashegawa M, Yagi K, Kondoh M. Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers. Eur J Pharm Biopharm. 2015;89:232-8 pubmed publisher
  23. Williams D, Anastos K, Morgello S, Berman J. JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals. J Leukoc Biol. 2015;97:401-12 pubmed publisher
  24. Huang J, Xu Y, Sun Z, Wang Z, Zhu Z, Song Y, et al. Low junctional adhesion molecule A expression correlates with poor prognosis in gastric cancer. J Surg Res. 2014;192:494-502 pubmed publisher
  25. Kohler E, Baruah J, Urao N, Ushio Fukai M, Fukai T, Chatterjee I, et al. Low-dose 6-bromoindirubin-3'-oxime induces partial dedifferentiation of endothelial cells to promote increased neovascularization. Stem Cells. 2014;32:1538-52 pubmed publisher