This is a Validated Antibody Database (VAD) review about human EphA4, based on 15 published articles (read how Labome selects the articles), using EphA4 antibody in all methods. It is aimed to help Labome visitors find the most suited EphA4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EphA4 synonym: EK8; HEK8; SEK; TYRO1

Invitrogen
mouse monoclonal (4C8H5)
  • western blot; mouse; 1:250; loading ...; fig 10a
Invitrogen EphA4 antibody (Invitrogen, 37-1600) was used in western blot on mouse samples at 1:250 (fig 10a). J Neurosci (2022) ncbi
mouse monoclonal (4C8H5)
  • western blot; human; loading ...; fig 7d
Invitrogen EphA4 antibody (ThermoFisher, 4C8H5) was used in western blot on human samples (fig 7d). J Virol (2018) ncbi
mouse monoclonal (4C8H5)
  • western blot; mouse; 1:1000; loading ...; fig 3a
In order to show a direct interaction between Sipa1l3 and Epha4 is required for proper eye development, Invitrogen EphA4 antibody (Invitrogen, 37-1600) was used in western blot on mouse samples at 1:1000 (fig 3a). Development (2017) ncbi
mouse monoclonal (4C8H5)
  • immunoprecipitation; human; loading ...; fig 4a
  • western blot; human; loading ...; fig 4a
In order to describe the contributions of ephrin-B1 and ephrin-B2 to the migration and proliferation of the intestinal stem cell niche, Invitrogen EphA4 antibody (Life Technologies, 37-1600) was used in immunoprecipitation on human samples (fig 4a) and in western blot on human samples (fig 4a). Exp Cell Res (2016) ncbi
mouse monoclonal (4C8H5)
  • western blot; mouse; 1:200
In order to investigate the role of EphA4 and EphrinA3 in adult axon regeneration using a model of adult mouse optic nerve injury, Invitrogen EphA4 antibody (Invitrogen, 37-1600) was used in western blot on mouse samples at 1:200. Eur J Neurosci (2014) ncbi
mouse monoclonal (4C8H5)
  • western blot; mouse; 1:200; fig 3
In order to study the increased levels of certain axon guidance proteins in the CNS of Nogo-A ablated mice and their role in limiting the outgrowth of neurites in the absence of Nogo, Invitrogen EphA4 antibody (Invitrogen, 37-1600) was used in western blot on mouse samples at 1:200 (fig 3). Eur J Neurosci (2013) ncbi
mouse monoclonal (4C8H5)
  • western blot; mouse; 1:1000; fig s2
In order to test if EphA4 affects stroke recovery and evaluate Eph44 as a therapeutic target, Invitrogen EphA4 antibody (Zymed, 37-1600) was used in western blot on mouse samples at 1:1000 (fig s2). Hum Mol Genet (2013) ncbi
mouse monoclonal (4C8H5)
  • immunohistochemistry; mouse
In order to investigate the role of Src-like adaptor protein in the context of NMDA receptor signaling, Invitrogen EphA4 antibody (Invitrogen, 37-1600) was used in immunohistochemistry on mouse samples . Mol Cell Biol (2013) ncbi
mouse monoclonal (4C8H5)
  • western blot; mouse; 1:1000; fig 4
In order to elucidate the role of Epha4 in models of amyotrophic lateral sclerosis, Invitrogen EphA4 antibody (Zymed, 37-1600) was used in western blot on mouse samples at 1:1000 (fig 4). Nat Med (2012) ncbi
mouse monoclonal (4C8H5)
  • immunoprecipitation; mouse; fig 5
In order to test if ADAM family proteins contribute to the development of the neuromuscular junction, Invitrogen EphA4 antibody (Zymed, 4C8H5) was used in immunoprecipitation on mouse samples (fig 5). PLoS ONE (2008) ncbi
mouse monoclonal (4C8H5)
  • western blot; mouse; fig 1
In order to examine neuronal RacGAP alpha2-chimaerin as an effector for the receptor tyrosine kinase EphA4 in cortical and spinal motor circuits, Invitrogen EphA4 antibody (Zymed, 4C8H5) was used in western blot on mouse samples (fig 1). Neuron (2007) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 8c
Abcam EphA4 antibody (Abcam, ab62256) was used in western blot on human samples at 1:500 (fig 8c). Oncogene (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3b
Abcam EphA4 antibody (Abcam, ab193214) was used in western blot on human samples (fig 3b). Breast Cancer Res (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (35)
  • western blot; rat
Santa Cruz Biotechnology EphA4 antibody (Santa Cruz Biotechnology, sc-135897) was used in western blot on rat samples . PLoS ONE (2014) ncbi
BD Biosciences
mouse monoclonal (35/EphA4)
  • immunohistochemistry - paraffin section; mouse; 1:100
BD Biosciences EphA4 antibody (BD Biosciences, 610471) was used in immunohistochemistry - paraffin section on mouse samples at 1:100. Development (2013) ncbi
Articles Reviewed
  1. Matsuura K, Kobayashi S, Konno K, Yamasaki M, Horiuchi T, Senda T, et al. SIPA1L1/SPAR1 Interacts with the Neurabin Family of Proteins and is Involved in GPCR Signaling. J Neurosci. 2022;42:2448-2473 pubmed publisher
  2. Ricci B, Millner T, Pomella N, Zhang X, Guglielmi L, Badodi S, et al. Polycomb-mediated repression of EphrinA5 promotes growth and invasion of glioblastoma. Oncogene. 2020;39:2523-2538 pubmed publisher
  3. Hori A, Shimoda M, Naoi Y, Kagara N, Tanei T, Miyake T, et al. Vasculogenic mimicry is associated with trastuzumab resistance of HER2-positive breast cancer. Breast Cancer Res. 2019;21:88 pubmed publisher
  4. TerBush A, Hafkamp F, Lee H, Coscoy L. A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins α3β1, αVβ3, and αVβ5. J Virol. 2018;92: pubmed publisher
  5. Rothe M, Kanwal N, Dietmann P, Seigfried F, Hempel A, Schütz D, et al. An Epha4/Sipa1l3/Wnt pathway regulates eye development and lens maturation. Development. 2017;144:321-333 pubmed publisher
  6. Jurek A, Genander M, Kundu P, Catchpole T, He X, Strååt K, et al. Eph receptor interclass cooperation is required for the regulation of cell proliferation. Exp Cell Res. 2016;348:10-22 pubmed publisher
  7. Joly S, Jordi N, Schwab M, Pernet V. The Ephrin receptor EphA4 restricts axonal sprouting and enhances branching in the injured mouse optic nerve. Eur J Neurosci. 2014;40:3021-31 pubmed publisher
  8. Vargas L, Leal N, Estrada L, González A, Serrano F, Araya K, et al. EphA4 activation of c-Abl mediates synaptic loss and LTP blockade caused by amyloid-β oligomers. PLoS ONE. 2014;9:e92309 pubmed publisher
  9. Kempf A, Montani L, Petrinovic M, Schroeter A, Weinmann O, Patrignani A, et al. Upregulation of axon guidance molecules in the adult central nervous system of Nogo-A knockout mice restricts neuronal growth and regeneration. Eur J Neurosci. 2013;38:3567-79 pubmed publisher
  10. Yang Z, Zimmerman S, BRAKEMAN P, Beaudoin G, Reichardt L, MARCIANO D. De novo lumen formation and elongation in the developing nephron: a central role for afadin in apical polarity. Development. 2013;140:1774-84 pubmed publisher
  11. Lemmens R, Jaspers T, Robberecht W, Thijs V. Modifying expression of EphA4 and its downstream targets improves functional recovery after stroke. Hum Mol Genet. 2013;22:2214-20 pubmed publisher
  12. Semerdjieva S, Abdul Razak H, Salim S, Yáñez Muñoz R, Chen P, Tarabykin V, et al. Activation of EphA receptors mediates the recruitment of the adaptor protein Slap, contributing to the downregulation of N-methyl-D-aspartate receptors. Mol Cell Biol. 2013;33:1442-55 pubmed publisher
  13. Van Hoecke A, Schoonaert L, Lemmens R, Timmers M, Staats K, Laird A, et al. EPHA4 is a disease modifier of amyotrophic lateral sclerosis in animal models and in humans. Nat Med. 2012;18:1418-22 pubmed
  14. Yumoto N, Wakatsuki S, Kurisaki T, Hara Y, Osumi N, Frisen J, et al. Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction. PLoS ONE. 2008;3:e3322 pubmed publisher
  15. Beg A, Sommer J, Martin J, Scheiffele P. alpha2-Chimaerin is an essential EphA4 effector in the assembly of neuronal locomotor circuits. Neuron. 2007;55:768-78 pubmed