This is a Validated Antibody Database (VAD) review about human EphA2, based on 26 published articles (read how Labome selects the articles), using EphA2 antibody in all methods. It is aimed to help Labome visitors find the most suited EphA2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EphA2 synonym: ARCC2; CTPA; CTPP1; CTRCT6; ECK

Knockout validation
Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; fig 4
In order to report the role for EPHA2 in the cell survival of tyrosine kinase inhibitors-resistant, EGFR-mutant lung cancers, Invitrogen EphA2 antibody (Life Technologies, 347400) was used in immunohistochemistry knockout validation on mouse samples (fig 4). Cancer Res (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4A2)
  • western blot knockout validation; human; loading ...; fig s6b
Cell Signaling Technology EphA2 antibody (Cell Signaling, 6997) was used in western blot knockout validation on human samples (fig s6b). MAbs (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4A2)
  • western blot knockout validation; human; loading ...; fig 1a
  • immunocytochemistry; human; fig 2f
Cell Signaling Technology EphA2 antibody (Cell Signaling, 6997) was used in western blot knockout validation on human samples (fig 1a) and in immunocytochemistry on human samples (fig 2f). Nat Microbiol (2018) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D7X2L)
  • western blot knockout validation; human; 1:1000; fig s4
In order to report the role for EPHA2 in the cell survival of tyrosine kinase inhibitors-resistant, EGFR-mutant lung cancers, Cell Signaling Technology EphA2 antibody (Cell Signaling Technology, 12677) was used in western blot knockout validation on human samples at 1:1000 (fig s4). Cancer Res (2016) ncbi
Haematologic Technologies
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; fig s4
In order to report the role for EPHA2 in the cell survival of tyrosine kinase inhibitors-resistant, EGFR-mutant lung cancers, Haematologic Technologies EphA2 antibody (Cell Applications, CY1108) was used in western blot knockout validation on human samples at 1:1000 (fig s4). Cancer Res (2016) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 2b
Invitrogen EphA2 antibody (Thermo Fisher, PA5-14574) was used in western blot on mouse samples (fig 2b). Respir Res (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 1a
In order to examine if EphA2 and EphB4 proteins are suitable targets for image-guided oncologic surgery of colorectal cancer, Invitrogen EphA2 antibody (Invitrogen, 34-7400) was used in immunohistochemistry - paraffin section on human samples (fig 1a). Int J Mol Sci (2017) ncbi
mouse monoclonal (1C11A12)
  • immunocytochemistry; human; 1:100; fig s1
  • immunocytochemistry; mouse; 1:100; fig 3
In order to describe the role of Anks1a in COPII-mediated EphA2 export from the endoplasmic reticulum, Invitrogen EphA2 antibody (Invitrogen, 37-4400) was used in immunocytochemistry on human samples at 1:100 (fig s1) and in immunocytochemistry on mouse samples at 1:100 (fig 3). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2a
  • western blot; mouse; loading ...; fig 1a
In order to assess the role of EphA2 signaling in mice with lipopolysaccharide-induced lung injury, Invitrogen EphA2 antibody (Thermo Fisher Scientific, PA5-14574) was used in immunohistochemistry - paraffin section on mouse samples (fig 2a) and in western blot on mouse samples (fig 1a). Clin Sci (Lond) (2016) ncbi
domestic rabbit polyclonal
  • proximity ligation assay; human; 1:350; loading ...; fig 3b
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 5a
  • western blot; human; 1:500; loading ...; fig 1a
In order to study the impact of ephrin signaling in non-small cell lung cancer, Invitrogen EphA2 antibody (Invitrogen, 34-7400) was used in proximity ligation assay on human samples at 1:350 (fig 3b), in immunohistochemistry - paraffin section on human samples at 1:200 (fig 5a) and in western blot on human samples at 1:500 (fig 1a). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 3f
  • western blot; human; loading ...; fig 3d
In order to screen for inhibitors of EphA2 canonical signaling, Invitrogen EphA2 antibody (Thermo Fisher, 34-7400) was used in immunoprecipitation on human samples (fig 3f) and in western blot on human samples (fig 3d). Mol Biol Cell (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; fig 4
In order to report the role for EPHA2 in the cell survival of tyrosine kinase inhibitors-resistant, EGFR-mutant lung cancers, Invitrogen EphA2 antibody (Life Technologies, 347400) was used in immunohistochemistry knockout validation on mouse samples (fig 4). Cancer Res (2016) ncbi
mouse monoclonal (1C11A12)
  • immunohistochemistry; human; fig 3
  • western blot; human; fig 1
In order to test if an anti-EphA2 monoclonal antibody linked to a chemotherapeutic agent has antitumor activity using ovarian cancer cell lines and tumor models, Invitrogen EphA2 antibody (Zymed, 1C11A12) was used in immunohistochemistry on human samples (fig 3) and in western blot on human samples (fig 1). J Natl Cancer Inst (2009) ncbi
mouse monoclonal (1C11A12)
  • western blot; human; 1:10,000; fig 3
In order to characterize monoclonal antibodies that recognize EphA2 and EphB4, Invitrogen EphA2 antibody (Invitrogen, 1C11A12) was used in western blot on human samples at 1:10,000 (fig 3). Mol Immunol (2007) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • immunohistochemistry; human; fig 1a
  • western blot; human; loading ...; fig 2a
Santa Cruz Biotechnology EphA2 antibody (Santa Cruz, sc-398832) was used in immunohistochemistry on human samples (fig 1a) and in western blot on human samples (fig 2a). Cell Rep (2021) ncbi
BioLegend
mouse monoclonal (SHM16)
  • flow cytometry; human; loading ...; fig 3a
BioLegend EphA2 antibody (BioLegend, SHM16) was used in flow cytometry on human samples (fig 3a). Nat Microbiol (2018) ncbi
mouse monoclonal (SHM16)
  • flow cytometry; human; 1:80; loading ...; fig 10
In order to identify human cytomegalovirus genes within the US12 family containing novel natural killer cell evasion functions, BioLegend EphA2 antibody (Biolegend, 356804) was used in flow cytometry on human samples at 1:80 (fig 10). elife (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4A2)
  • western blot knockout validation; human; loading ...; fig s6b
Cell Signaling Technology EphA2 antibody (Cell Signaling, 6997) was used in western blot knockout validation on human samples (fig s6b). MAbs (2021) ncbi
domestic rabbit monoclonal (D7X2L)
  • immunohistochemistry; human; fig 1a
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology EphA2 antibody (Cell signaling, 12677S) was used in immunohistochemistry on human samples (fig 1a) and in western blot on human samples (fig 2a). Cell Rep (2021) ncbi
domestic rabbit monoclonal (D4A2)
  • western blot; human; loading ...; fig 1, 2
Cell Signaling Technology EphA2 antibody (Cell Signaling Technology, clone D4A2; #6997;) was used in western blot on human samples (fig 1, 2). Cells (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2
Cell Signaling Technology EphA2 antibody (Cell Signaling Technology, #8244) was used in western blot on human samples (fig 2). Cells (2020) ncbi
domestic rabbit monoclonal (D9A1)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 2a
Cell Signaling Technology EphA2 antibody (Cell Signalling, D9A1) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 2a). Breast Cancer Res Treat (2020) ncbi
domestic rabbit monoclonal (D9A1)
  • western blot; human; loading ...; fig 3b
Cell Signaling Technology EphA2 antibody (Cell Signaling Technology, 6347) was used in western blot on human samples (fig 3b). Breast Cancer Res (2019) ncbi
domestic rabbit monoclonal (D4A2)
  • western blot; human; loading ...; fig 3b
Cell Signaling Technology EphA2 antibody (Cell Signaling Technology, 6997) was used in western blot on human samples (fig 3b). Breast Cancer Res (2019) ncbi
domestic rabbit monoclonal (D4A2)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig e1f
Cell Signaling Technology EphA2 antibody (Cell Signaling, 6997) was used in immunohistochemistry - paraffin section on mouse samples (fig e1f). Nature (2019) ncbi
domestic rabbit monoclonal (D4A2)
  • western blot knockout validation; human; loading ...; fig 1a
  • immunocytochemistry; human; fig 2f
Cell Signaling Technology EphA2 antibody (Cell Signaling, 6997) was used in western blot knockout validation on human samples (fig 1a) and in immunocytochemistry on human samples (fig 2f). Nat Microbiol (2018) ncbi
domestic rabbit monoclonal (D9A1)
  • immunocytochemistry; human; 1:200; loading ...; fig 6b
  • western blot; human; 1:1000; loading ...; fig 6d
In order to elucidate the mechanisms by which Rab-coupling protein contributes to metastasis, Cell Signaling Technology EphA2 antibody (Cell Signaling, 6347) was used in immunocytochemistry on human samples at 1:200 (fig 6b) and in western blot on human samples at 1:1000 (fig 6d). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D7X2L)
  • western blot; human; 1:1000; loading ...; fig s6b
In order to elucidate the mechanisms by which Rab-coupling protein contributes to metastasis, Cell Signaling Technology EphA2 antibody (Cell Signaling, 12677) was used in western blot on human samples at 1:1000 (fig s6b). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D4A2)
  • immunocytochemistry; human; 1:100; loading ...; tbl 1
Cell Signaling Technology EphA2 antibody (cell signalling, Mab6997) was used in immunocytochemistry on human samples at 1:100 (tbl 1). PLoS ONE (2017) ncbi
domestic rabbit monoclonal (D4A2)
  • immunocytochemistry; human; loading ...; fig 5b
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology EphA2 antibody (cell signalling, 6997) was used in immunocytochemistry on human samples (fig 5b) and in western blot on human samples (fig 5a). PLoS Pathog (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:100; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology EphA2 antibody (Cell Signaling, 3970) was used in western blot on human samples at 1:100 (fig st1). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D4A2)
  • western blot; human; 1:100; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology EphA2 antibody (Cell Signaling, 6997P) was used in western blot on human samples at 1:100 (fig st1). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D7X2L)
  • western blot; human; loading ...; fig 3h
In order to screen for inhibitors of EphA2 canonical signaling, Cell Signaling Technology EphA2 antibody (Cell Signaling, 12677) was used in western blot on human samples (fig 3h). Mol Biol Cell (2016) ncbi
domestic rabbit monoclonal (D9A1)
  • immunoprecipitation; human; loading ...; fig 3c
  • western blot; human; loading ...; fig 3a
In order to screen for inhibitors of EphA2 canonical signaling, Cell Signaling Technology EphA2 antibody (Cell Signaling, 6347) was used in immunoprecipitation on human samples (fig 3c) and in western blot on human samples (fig 3a). Mol Biol Cell (2016) ncbi
domestic rabbit monoclonal (D9A1)
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology EphA2 antibody (Cell Signaling Technology, 6347) was used in western blot on human samples (fig 5a). BMC Cancer (2016) ncbi
domestic rabbit monoclonal (D7X2L)
  • western blot; human; loading ...; fig 6
Cell Signaling Technology EphA2 antibody (Cell Signaling Technology, 12677) was used in western blot on human samples (fig 6). BMC Cancer (2016) ncbi
domestic rabbit monoclonal (D4A2)
  • immunohistochemistry - paraffin section; human; loading ...; fig 7
  • western blot; human; loading ...; fig 1a,2c
Cell Signaling Technology EphA2 antibody (Cell Signaling Technology, 6997) was used in immunohistochemistry - paraffin section on human samples (fig 7) and in western blot on human samples (fig 1a,2c). BMC Cancer (2016) ncbi
domestic rabbit monoclonal (D7X2L)
  • western blot knockout validation; human; 1:1000; fig s4
In order to report the role for EPHA2 in the cell survival of tyrosine kinase inhibitors-resistant, EGFR-mutant lung cancers, Cell Signaling Technology EphA2 antibody (Cell Signaling Technology, 12677) was used in western blot knockout validation on human samples at 1:1000 (fig s4). Cancer Res (2016) ncbi
domestic rabbit monoclonal (D4A2)
  • western blot; human; 1:1000; fig 5
Cell Signaling Technology EphA2 antibody (Cell Signaling, 6997P) was used in western blot on human samples at 1:1000 (fig 5). Cancer Sci (2015) ncbi
domestic rabbit monoclonal (D4A2)
  • western blot; human
In order to identify Eph tyrosine kinase receptors as the substrates of tissue factor/coagulation factor VIIa, Cell Signaling Technology EphA2 antibody (Cell Signaling, 6997) was used in western blot on human samples . J Biol Chem (2014) ncbi
domestic rabbit monoclonal (D4A2)
  • western blot; human; 1:1000; fig s6
In order to study juxtacrine signaling from macrophages and monocytes and a breast cancer stem cell niche, Cell Signaling Technology EphA2 antibody (Cell Signaling, 6997) was used in western blot on human samples at 1:1000 (fig s6). Nat Cell Biol (2014) ncbi
domestic rabbit monoclonal (D7X2L)
  • western blot; human; 1:1000; fig 6
In order to study juxtacrine signaling from macrophages and monocytes and a breast cancer stem cell niche, Cell Signaling Technology EphA2 antibody (Cell Signaling, 12677) was used in western blot on human samples at 1:1000 (fig 6). Nat Cell Biol (2014) ncbi
Haematologic Technologies
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 3c
  • western blot; human; loading ...; fig 3a
In order to screen for inhibitors of EphA2 canonical signaling, Haematologic Technologies EphA2 antibody (Cell Applications, CY1108) was used in immunoprecipitation on human samples (fig 3c) and in western blot on human samples (fig 3a). Mol Biol Cell (2016) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; fig s4
In order to report the role for EPHA2 in the cell survival of tyrosine kinase inhibitors-resistant, EGFR-mutant lung cancers, Haematologic Technologies EphA2 antibody (Cell Applications, CY1108) was used in western blot knockout validation on human samples at 1:1000 (fig s4). Cancer Res (2016) ncbi
Articles Reviewed
  1. Lee S, Shin M, Leem A, Lee S, Chung K, Kim Y, et al. NADPH oxidase 4 signaling in a ventilator-induced lung injury mouse model. Respir Res. 2022;23:73 pubmed publisher
  2. Enderle L, Shalaby K, Gorelik M, Weiss A, Blazer L, Paduch M, et al. A T cell redirection platform for co-targeting dual antigens on solid tumors. MAbs. 2021;13:1933690 pubmed publisher
  3. Wang H, Hou W, Perera A, Bettler C, Beach J, Ding X, et al. Targeting EphA2 suppresses hepatocellular carcinoma initiation and progression by dual inhibition of JAK1/STAT3 and AKT signaling. Cell Rep. 2021;34:108765 pubmed publisher
  4. Leite M, Marques M, Melo J, Pinto M, Cavadas B, Aroso M, et al. Helicobacter Pylori Targets the EPHA2 Receptor Tyrosine Kinase in Gastric Cells Modulating Key Cellular Functions. Cells. 2020;9: pubmed publisher
  5. Mitra D, Bhattacharyya S, Alam N, Sen S, Mitra S, Mandal S, et al. Phosphorylation of EphA2 receptor and vasculogenic mimicry is an indicator of poor prognosis in invasive carcinoma of the breast. Breast Cancer Res Treat. 2020;179:359-370 pubmed publisher
  6. Hori A, Shimoda M, Naoi Y, Kagara N, Tanei T, Miyake T, et al. Vasculogenic mimicry is associated with trastuzumab resistance of HER2-positive breast cancer. Breast Cancer Res. 2019;21:88 pubmed publisher
  7. Yao W, Rose J, Wang W, Seth S, Jiang H, Taguchi A, et al. Syndecan 1 is a critical mediator of macropinocytosis in pancreatic cancer. Nature. 2019;: pubmed publisher
  8. Chen J, Sathiyamoorthy K, Zhang X, Schaller S, Perez White B, Jardetzky T, et al. Ephrin receptor A2 is a functional entry receptor for Epstein-Barr virus. Nat Microbiol. 2018;3:172-180 pubmed publisher
  9. Zhang H, Li Y, Wang H, Zhang A, Chen M, Fang Z, et al. Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry. Nat Microbiol. 2018;3:1-8 pubmed publisher
  10. Gundry C, Marco S, Rainero E, Miller B, Dornier E, Mitchell L, et al. Phosphorylation of Rab-coupling protein by LMTK3 controls Rab14-dependent EphA2 trafficking to promote cell:cell repulsion. Nat Commun. 2017;8:14646 pubmed publisher
  11. Gomes de Castro M, Hobartner C, Opazo F. Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy. PLoS ONE. 2017;12:e0173050 pubmed publisher
  12. Fielding C, Weekes M, Nobre L, Růcková E, Wilkie G, Paulo J, et al. Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation. elife. 2017;6: pubmed publisher
  13. Stammes M, Prevoo H, Ter Horst M, Groot S, Van De Velde C, Chan A, et al. Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery. Int J Mol Sci. 2017;18: pubmed publisher
  14. Kumar B, Dutta D, Iqbal J, Ansari M, Roy A, Chikoti L, et al. ESCRT-I Protein Tsg101 Plays a Role in the Post-macropinocytic Trafficking and Infection of Endothelial Cells by Kaposi's Sarcoma-Associated Herpesvirus. PLoS Pathog. 2016;12:e1005960 pubmed publisher
  15. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  16. Lee H, Noh H, Mun J, Gu C, Sever S, Park S. Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis. Nat Commun. 2016;7:12799 pubmed publisher
  17. Hong J, Shin M, Douglas I, Chung K, Kim E, Jung J, et al. Inhibition of EphA2/EphrinA1 signal attenuates lipopolysaccharide-induced lung injury. Clin Sci (Lond). 2016;130:1993-2003 pubmed
  18. Efazat G, Novak M, Kaminskyy V, De Petris L, Kanter L, Juntti T, et al. Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer. Oncotarget. 2016;7:60332-60347 pubmed publisher
  19. Barquilla A, Lamberto I, Noberini R, Heynen Genel S, Brill L, Pasquale E. Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation. Mol Biol Cell. 2016;27:2757-70 pubmed publisher
  20. Eriksson O, Thulin A, Asplund A, Hegde G, Navani S, Siegbahn A. Cross-talk between the Tissue Factor/coagulation factor VIIa complex and the tyrosine kinase receptor EphA2 in cancer. BMC Cancer. 2016;16:341 pubmed publisher
  21. Amato K, Wang S, Tan L, Hastings A, Song W, Lovly C, et al. EPHA2 Blockade Overcomes Acquired Resistance to EGFR Kinase Inhibitors in Lung Cancer. Cancer Res. 2016;76:305-18 pubmed publisher
  22. Liu X, Wang J, Li S, Li L, Huang M, Zhang Y, et al. Histone deacetylase 3 expression correlates with vasculogenic mimicry through the phosphoinositide3-kinase / ERK-MMP-laminin5γ2 signaling pathway. Cancer Sci. 2015;106:857-66 pubmed publisher
  23. Eriksson O, Ramström M, Hörnaeus K, Bergquist J, Mokhtari D, Siegbahn A. The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa. J Biol Chem. 2014;289:32379-91 pubmed publisher
  24. Lu H, Clauser K, Tam W, Fröse J, Ye X, Eaton E, et al. A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages. Nat Cell Biol. 2014;16:1105-17 pubmed publisher
  25. Lee J, Han H, Shahzad M, Kim S, Mangala L, Nick A, et al. EphA2 immunoconjugate as molecularly targeted chemotherapy for ovarian carcinoma. J Natl Cancer Inst. 2009;101:1193-205 pubmed publisher
  26. Damschroder M, Widjaja L, Gill P, Krasnoperov V, Jiang W, Dall Acqua W, et al. Framework shuffling of antibodies to reduce immunogenicity and manipulate functional and biophysical properties. Mol Immunol. 2007;44:3049-60 pubmed