This is a Validated Antibody Database (VAD) review about human EWS, based on 8 published articles (read how Labome selects the articles), using EWS antibody in all methods. It is aimed to help Labome visitors find the most suited EWS antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EWS synonym: EWS; EWS-FLI1; bK984G1.4; RNA-binding protein EWS; EWS RNA-binding protein variant 6; Ewing sarcoma breakpoint region 1; Ewings sarcoma EWS-Fli1 (type 1) oncogene

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (G-5)
  • immunocytochemistry knockout validation; mouse; fig 2
  • western blot knockout validation; mouse; fig 1
Santa Cruz Biotechnology EWS antibody (Santa Cruz, sc-28327) was used in immunocytochemistry knockout validation on mouse samples (fig 2) and in western blot knockout validation on mouse samples (fig 1). Autophagy (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-5)
  • western blot; human; 1:1000; fig 2
In order to study the affects in the subcellular localization of Drosha by alternative splicing, Santa Cruz Biotechnology EWS antibody (Santa Cruz, sc-28327) was used in western blot on human samples at 1:1000 (fig 2). Nucleic Acids Res (2016) ncbi
mouse monoclonal (C-9)
  • immunocytochemistry; human; fig 3
In order to characterize an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications defined by PLEKHA7, Santa Cruz Biotechnology EWS antibody (Santa Cruz, sc-48404) was used in immunocytochemistry on human samples (fig 3). Cell Cycle (2016) ncbi
mouse monoclonal (C-9)
  • ChIP-Seq; human; fig 2
  • western blot; human; 1:2000; fig s10
Santa Cruz Biotechnology EWS antibody (santa Cruz, sc-48404) was used in ChIP-Seq on human samples (fig 2) and in western blot on human samples at 1:2000 (fig s10). BMC Genomics (2015) ncbi
mouse monoclonal (G-5)
  • immunocytochemistry knockout validation; mouse; fig 2
  • western blot knockout validation; mouse; fig 1
Santa Cruz Biotechnology EWS antibody (Santa Cruz, sc-28327) was used in immunocytochemistry knockout validation on mouse samples (fig 2) and in western blot knockout validation on mouse samples (fig 1). Autophagy (2015) ncbi
mouse monoclonal (C-9)
  • western blot; human
Santa Cruz Biotechnology EWS antibody (Santa-Cruz, sc-48404) was used in western blot on human samples . Oncogene (2013) ncbi
Abcam
rabbit monoclonal (EPR4647)
  • western blot; mouse; 1:5000; loading ...; fig 3a
Abcam EWS antibody (Abcam, ab133288) was used in western blot on mouse samples at 1:5000 (fig 3a). Acta Neuropathol Commun (2018) ncbi
GeneTex
rabbit polyclonal
  • RNA immunoprecipitation; human
In order to develop reagents to study RNA binding proteins, GeneTex EWS antibody (GeneTex, GTX114069) was used in RNA immunoprecipitation on human samples . Mol Cell (2016) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig 6a
In order to report the O-linked N-acetylglucosamine glycoproteome observed in human T cells, Cell Signaling Technology EWS antibody (Cell Signaling, 11910) was used in western blot on human samples (fig 6a). J Immunol (2016) ncbi
Articles Reviewed
  1. Maziuk B, Apicco D, Cruz A, Jiang L, Ash P, da Rocha E, et al. RNA binding proteins co-localize with small tau inclusions in tauopathy. Acta Neuropathol Commun. 2018;6:71 pubmed publisher
  2. Lund P, Elias J, Davis M. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol. 2016;197:3086-3098 pubmed
  3. Link S, Grund S, Diederichs S. Alternative splicing affects the subcellular localization of Drosha. Nucleic Acids Res. 2016;44:5330-43 pubmed publisher
  4. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  5. Kourtidis A, Anastasiadis P. PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications. Cell Cycle. 2016;15:498-505 pubmed publisher
  6. Luo Y, Blechingberg J, Fernandes A, Li S, Fryland T, Børglum A, et al. EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions. BMC Genomics. 2015;16:929 pubmed publisher
  7. Kim Y, Kang Y, Lee N, Kim K, Hwang Y, Kim H, et al. Uvrag targeting by Mir125a and Mir351 modulates autophagy associated with Ewsr1 deficiency. Autophagy. 2015;11:796-811 pubmed publisher
  8. Sankar S, Bell R, Stephens B, Zhuo R, Sharma S, Bearss D, et al. Mechanism and relevance of EWS/FLI-mediated transcriptional repression in Ewing sarcoma. Oncogene. 2013;32:5089-100 pubmed publisher