This is a Validated Antibody Database (VAD) review about human ERK5, based on 16 published articles (read how Labome selects the articles), using ERK5 antibody in all methods. It is aimed to help Labome visitors find the most suited ERK5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ERK5 synonym: BMK1; ERK4; ERK5; PRKM7

Santa Cruz Biotechnology
mouse monoclonal (36.T218/Y220)
  • ELISA; human; fig 4
Santa Cruz Biotechnology ERK5 antibody (Santa, sc-135761) was used in ELISA on human samples (fig 4). BMC Cancer (2015) ncbi
mouse monoclonal (1.T218/Y220)
  • chromatin immunoprecipitation; human
Santa Cruz Biotechnology ERK5 antibody (Santa Cruz Biotechnology, sc-135760) was used in chromatin immunoprecipitation on human samples . Mol Cancer Res (2014) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; fig 3
Abcam ERK5 antibody (Abcam, ab196609) was used in western blot on human samples (fig 3). Oncol Lett (2016) ncbi
domestic rabbit monoclonal (EP791Y)
  • western blot; human; 1:1000
Abcam ERK5 antibody (Abcam, ab40809) was used in western blot on human samples at 1:1000. J Cell Biochem (2015) ncbi
R&D Systems
domestic goat polyclonal
  • immunoprecipitation; human; fig 5e
In order to study the role of statin and ERK5 in tight junction formation and function in endothelial cells, R&D Systems ERK5 antibody (R&D systems, AF2848) was used in immunoprecipitation on human samples (fig 5e). J Cell Physiol (2018) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 6f
In order to demonstrate that TORC1 is important for proteasome homeostasis, MilliporeSigma ERK5 antibody (Sigma, E1523) was used in western blot on human samples at 1:1000 (fig 6f). Nature (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 5d
Cell Signaling Technology ERK5 antibody (Cell Signaling, 3371) was used in western blot on mouse samples (fig 5d). JACC Basic Transl Sci (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig s3c
Cell Signaling Technology ERK5 antibody (Cell Signaling, 3372) was used in western blot on human samples at 1:1000 (fig s3c). Nat Commun (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 6e
Cell Signaling Technology ERK5 antibody (Cell signaling, 3371) was used in western blot on human samples (fig 6e). PLoS Biol (2018) ncbi
domestic rabbit monoclonal (D23E9)
  • western blot; human; loading ...; fig 6e
Cell Signaling Technology ERK5 antibody (Cell signaling, 3552) was used in western blot on human samples (fig 6e). PLoS Biol (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 2a
Cell Signaling Technology ERK5 antibody (Cell Signaling, 3372) was used in western blot on mouse samples at 1:1000 (fig 2a). Proc Natl Acad Sci U S A (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 5d
Cell Signaling Technology ERK5 antibody (Cell Signaling, 3371) was used in western blot on human samples (fig 5d). Mol Oncol (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 7
  • western blot; human; fig 1b
In order to study the role of statin and ERK5 in tight junction formation and function in endothelial cells, Cell Signaling Technology ERK5 antibody (New England Biolabs, 3372) was used in immunocytochemistry on human samples (fig 7) and in western blot on human samples (fig 1b). J Cell Physiol (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 3a
In order to study the role of statin and ERK5 in tight junction formation and function in endothelial cells, Cell Signaling Technology ERK5 antibody (New England Biolabs, 3371) was used in western blot on human samples (fig 3a). J Cell Physiol (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2f
Cell Signaling Technology ERK5 antibody (Cell Signaling Technology, 3371) was used in western blot on human samples (fig 2f). Biochim Biophys Acta (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 6d
Cell Signaling Technology ERK5 antibody (Cell Signaling Technology, 3372) was used in western blot on human samples (fig 6d). Biochim Biophys Acta (2016) ncbi
domestic rabbit monoclonal (D23E9)
  • western blot; human; fig s7
  • western blot; mouse; fig 3
In order to analyze the rescue of intestinal epithelial turnover and tumour cell proliferation upon ERK1/2 abrogation due to ERK5 signaling, Cell Signaling Technology ERK5 antibody (Cell signaling, D23E9) was used in western blot on human samples (fig s7) and in western blot on mouse samples (fig 3). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 8c
In order to assess the contribution of MEK5/ERK5 signaling in the sensitivity of colon cancer cells to 5-fluorouracil, Cell Signaling Technology ERK5 antibody (Cell Signaling Technology, 3372) was used in western blot on human samples (fig 8c). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D23E9)
  • western blot; human; 1:1000; fig s1
Cell Signaling Technology ERK5 antibody (Cell Signaling Technology, 3552) was used in western blot on human samples at 1:1000 (fig s1). PLoS ONE (2016) ncbi
domestic rabbit monoclonal (D23E9)
  • western blot; human; fig 3
Cell Signaling Technology ERK5 antibody (Cell Signaling, 3552) was used in western blot on human samples (fig 3). BMC Cancer (2015) ncbi
EMD Millipore
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig s7d
EMD Millipore ERK5 antibody (Millipore, 07-507) was used in immunohistochemistry on mouse samples at 1:200 (fig s7d). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 3
  • western blot; mouse; fig 3
In order to analyze the rescue of intestinal epithelial turnover and tumour cell proliferation upon ERK1/2 abrogation due to ERK5 signaling, EMD Millipore ERK5 antibody (Millipore, 07-507) was used in western blot on human samples (fig 3) and in western blot on mouse samples (fig 3). Nat Commun (2016) ncbi
Articles Reviewed
  1. Guo X, Kolpakov M, Hooshdaran B, Schappell W, Wang T, Eguchi S, et al. Cardiac Expression of Factor X Mediates Cardiac Hypertrophy and Fibrosis in Pressure Overload. JACC Basic Transl Sci. 2020;5:69-83 pubmed publisher
  2. Kennedy S, Jarboui M, Srihari S, Raso C, Bryan K, Dernayka L, et al. Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRASG13D. Nat Commun. 2020;11:499 pubmed publisher
  3. Tan P, Ye Y, He L, Xie J, Jing J, Ma G, et al. TRIM59 promotes breast cancer motility by suppressing p62-selective autophagic degradation of PDCD10. PLoS Biol. 2018;16:e3000051 pubmed publisher
  4. Giurisato E, Xu Q, Lonardi S, Telfer B, Russo I, Pearson A, et al. Myeloid ERK5 deficiency suppresses tumor growth by blocking protumor macrophage polarization via STAT3 inhibition. Proc Natl Acad Sci U S A. 2018;115:E2801-E2810 pubmed publisher
  5. Fang J, Coon B, Gillis N, Chen Z, Qiu J, Chittenden T, et al. Shear-induced Notch-Cx37-p27 axis arrests endothelial cell cycle to enable arterial specification. Nat Commun. 2017;8:2149 pubmed publisher
  6. Baumann C, Ullrich A, Torka R. GAS6-expressing and self-sustaining cancer cells in 3D spheroids activate the PDK-RSK-mTOR pathway for survival and drug resistance. Mol Oncol. 2017;11:1430-1447 pubmed publisher
  7. Wilkinson E, Sidaway J, Cross M. Statin regulated ERK5 stimulates tight junction formation and reduces permeability in human cardiac endothelial cells. J Cell Physiol. 2018;233:186-200 pubmed publisher
  8. Rousseau A, Bertolotti A. An evolutionarily conserved pathway controls proteasome homeostasis. Nature. 2016;536:184-9 pubmed
  9. Shi K, Qian J, Qi L, Mao D, Chen Y, Zhu Y, et al. Atorvastatin antagonizes the visfatin-induced expression of inflammatory mediators via the upregulation of NF-?B activation in HCAECs. Oncol Lett. 2016;12:1438-1444 pubmed
  10. Im J, Yoon S, Kim B, Ban H, Won K, Chung K, et al. DNA damage induced apoptosis suppressor (DDIAS) is upregulated via ERK5/MEF2B signaling and promotes ?-catenin-mediated invasion. Biochim Biophys Acta. 2016;1859:1449-1458 pubmed publisher
  11. de Jong P, Taniguchi K, Harris A, Bertin S, Takahashi N, Duong J, et al. ERK5 signalling rescues intestinal epithelial turnover and tumour cell proliferation upon ERK1/2 abrogation. Nat Commun. 2016;7:11551 pubmed publisher
  12. Pereira D, Simões A, Gomes S, Castro R, Carvalho T, Rodrigues C, et al. MEK5/ERK5 signaling inhibition increases colon cancer cell sensitivity to 5-fluorouracil through a p53-dependent mechanism. Oncotarget. 2016;7:34322-40 pubmed publisher
  13. Qiu Z, Sun R, Mo X, Li W. The p70S6K Specific Inhibitor PF-4708671 Impedes Non-Small Cell Lung Cancer Growth. PLoS ONE. 2016;11:e0147185 pubmed publisher
  14. Gavine P, Wang M, Yu D, Hu E, Huang C, Xia J, et al. Identification and validation of dysregulated MAPK7 (ERK5) as a novel oncogenic target in squamous cell lung and esophageal carcinoma. BMC Cancer. 2015;15:454 pubmed publisher
  15. Zuo Y, Wu Y, Wehrli B, Chakrabarti S, Chakraborty C. Modulation of ERK5 is a novel mechanism by which Cdc42 regulates migration of breast cancer cells. J Cell Biochem. 2015;116:124-32 pubmed publisher
  16. Madak Erdogan Z, Ventrella R, Petry L, Katzenellenbogen B. Novel roles for ERK5 and cofilin as critical mediators linking ER?-driven transcription, actin reorganization, and invasiveness in breast cancer. Mol Cancer Res. 2014;12:714-27 pubmed publisher