This is a Validated Antibody Database (VAD) review about human EIF4A3, based on 13 published articles (read how Labome selects the articles), using EIF4A3 antibody in all methods. It is aimed to help Labome visitors find the most suited EIF4A3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EIF4A3 synonym: DDX48; Fal1; MUK34; NMP265; NUK34; RCPS; eIF4AIII; eukaryotic initiation factor 4A-III; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; NMP 265; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-like NUK-34; eukaryotic translation initiation factor 4A; hNMP 265; nuclear matrix protein 265

Abcam
rabbit polyclonal
  • immunocytochemistry; mouse; 1:50; loading ...; fig s4a
Abcam EIF4A3 antibody (Abcam, Ab32485) was used in immunocytochemistry on mouse samples at 1:50 (fig s4a). Biol Reprod (2018) ncbi
rabbit monoclonal (EPR14301(B))
  • immunocytochemistry; human; fig 5a
Abcam EIF4A3 antibody (Abcam, ab180573) was used in immunocytochemistry on human samples (fig 5a). PLoS Genet (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 4a
In order to investigate how FYCO1 contributes to the integrity of the chromatoid body, Abcam EIF4A3 antibody (Abcam, ab32485) was used in western blot on mouse samples (fig 4a). Autophagy (2017) ncbi
rabbit polyclonal
  • western blot; mouse; fig s1
Abcam EIF4A3 antibody (Abcam, Ab32485) was used in western blot on mouse samples (fig s1). Nucleic Acids Res (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 1
Abcam EIF4A3 antibody (Abcam, ab32485) was used in western blot on human samples (fig 1). Cancer Discov (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-2)
  • western blot; human; 1:1000; loading ...; fig 2c
In order to identify splicing factors as translation regulators during mitosis, Santa Cruz Biotechnology EIF4A3 antibody (Santa Cruz Biotechnology, sc-365549) was used in western blot on human samples at 1:1000 (fig 2c). Nucleic Acids Res (2017) ncbi
mouse monoclonal (B-2)
  • immunoprecipitation; human
  • western blot; human
Santa Cruz Biotechnology EIF4A3 antibody (Santa Cruz Biotechnology, sc-365549) was used in immunoprecipitation on human samples and in western blot on human samples . Mol Cell Biol (2015) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 4c
Bethyl EIF4A3 antibody (Bethyl Laboratories, A302-981A) was used in western blot on human samples at 1:500 (fig 4c). elife (2018) ncbi
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl EIF4A3 antibody (Bethyl, A302-981A) was used in RNA immunoprecipitation on human samples and in immunocytochemistry on human samples at 2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 3
Bethyl EIF4A3 antibody (Bethyl Laboratories, A302-980A) was used in western blot on human samples (fig 3). Virology (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; 1:2000; loading ...; fig s3b
Proteintech Group EIF4A3 antibody (Proteintech, 17504-1-AP) was used in western blot on human samples at 1:2000 (fig s3b). Cell Res (2017) ncbi
rabbit polyclonal
  • western blot; human; fig 4a
In order to identify residues of Y14 that are needed for binding to the mRNA cap, Proteintech Group EIF4A3 antibody (ProteinTech, 17504-1-AP) was used in western blot on human samples (fig 4a). J Biol Chem (2016) ncbi
Invitrogen
rabbit polyclonal
  • other; human; 1:1000; fig 4
In order to elucidate the roles of nuclear cap-binding protein 1-3 in mRNA biogenesis, Invitrogen EIF4A3 antibody (Thermo Scientific, PA5-30329) was used in other on human samples at 1:1000 (fig 4). Nat Commun (2015) ncbi
Articles Reviewed
  1. Baird T, Cheng K, Chen Y, Buehler E, Martin S, Inglese J, et al. ICE1 promotes the link between splicing and nonsense-mediated mRNA decay. elife. 2018;7: pubmed publisher
  2. Hu J, Sun F, Handel M. Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice. Biol Reprod. 2018;98:102-114 pubmed publisher
  3. Aviner R, Hofmann S, Elman T, Shenoy A, Geiger T, Elkon R, et al. Proteomic analysis of polyribosomes identifies splicing factors as potential regulators of translation during mitosis. Nucleic Acids Res. 2017;45:5945-5957 pubmed publisher
  4. Shadle S, Zhong J, Campbell A, Conerly M, Jagannathan S, Wong C, et al. DUX4-induced dsRNA and MYC mRNA stabilization activate apoptotic pathways in human cell models of facioscapulohumeral dystrophy. PLoS Genet. 2017;13:e1006658 pubmed publisher
  5. Shi H, Wang X, Lu Z, Zhao B, Ma H, Hsu P, et al. YTHDF3 facilitates translation and decay of N6-methyladenosine-modified RNA. Cell Res. 2017;27:315-328 pubmed publisher
  6. Da Ros M, Lehtiniemi T, Olotu O, Fischer D, Zhang F, Vihinen H, et al. FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules. Autophagy. 2017;13:302-321 pubmed publisher
  7. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  8. Chuang T, Lee K, Lou Y, Lu C, Tarn W. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. J Biol Chem. 2016;291:8565-74 pubmed publisher
  9. Ziehr B, Lenarcic E, Cecil C, Moorman N. The eIF4AIII RNA helicase is a critical determinant of human cytomegalovirus replication. Virology. 2016;489:194-201 pubmed publisher
  10. Sanchez G, Bondy Chorney E, Laframboise J, Paris G, Didillon A, Jasmin B, et al. A novel role for CARM1 in promoting nonsense-mediated mRNA decay: potential implications for spinal muscular atrophy. Nucleic Acids Res. 2016;44:2661-76 pubmed publisher
  11. Gebhardt A, Habjan M, Benda C, Meiler A, Haas D, Hein M, et al. mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3. Nat Commun. 2015;6:8192 pubmed publisher
  12. Van Rechem C, Black J, Boukhali M, Aryee M, Gräslund S, Haas W, et al. Lysine demethylase KDM4A associates with translation machinery and regulates protein synthesis. Cancer Discov. 2015;5:255-63 pubmed publisher
  13. Eifler T, Shao W, Bartholomeeusen K, Fujinaga K, Jäger S, Johnson J, et al. Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts. Mol Cell Biol. 2015;35:468-78 pubmed publisher