This is a Validated Antibody Database (VAD) review about human EIF2AK4, based on 17 published articles (read how Labome selects the articles), using EIF2AK4 antibody in all methods. It is aimed to help Labome visitors find the most suited EIF2AK4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EIF2AK4 synonym: GCN2; PVOD2

Knockout validation
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 2b
Cell Signaling Technology EIF2AK4 antibody (Cell Signaling, 3302) was used in western blot knockout validation on mouse samples (fig 2b). PLoS Biol (2021) ncbi
Abcam
domestic rabbit monoclonal (EPR2320Y)
  • western blot; human; loading ...; fig 1a
  • western blot; mouse; loading ...; fig 1a, 5a
Abcam EIF2AK4 antibody (Abcam, Ab75836) was used in western blot on human samples (fig 1a) and in western blot on mouse samples (fig 1a, 5a). Nucleic Acids Res (2021) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; mouse; loading ...
Abcam EIF2AK4 antibody (Abcam, ab75836) was used in western blot on mouse samples . PLoS Biol (2021) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; mouse; 1:1000; loading ...; fig 1a
Abcam EIF2AK4 antibody (Abcam, ab75836) was used in western blot on mouse samples at 1:1000 (fig 1a). elife (2020) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 3g
  • western blot; human; 1:500; loading ...; fig 5d
Abcam EIF2AK4 antibody (Abcam, Ab75836) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 3g) and in western blot on human samples at 1:500 (fig 5d). elife (2020) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; human; 1:500; fig 4l
Abcam EIF2AK4 antibody (Abcam, ab75836) was used in western blot on human samples at 1:500 (fig 4l). elife (2020) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; hamsters; 1:1000; loading ...; fig 3d
Abcam EIF2AK4 antibody (AbCam, ab75836) was used in western blot on hamsters samples at 1:1000 (fig 3d). elife (2019) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; mouse; 1:1000; loading ...; fig s4b
Abcam EIF2AK4 antibody (Abcam, ab75836) was used in western blot on mouse samples at 1:1000 (fig s4b). Science (2019) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; mouse; 1:1000; loading ...; fig 1a
In order to propose that GCN2 is an important sensor of the state of the actin cytoskeleton, Abcam EIF2AK4 antibody (Abcam, ab75836) was used in western blot on mouse samples at 1:1000 (fig 1a). J Cell Sci (2016) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; human; 1:500; fig 2
In order to demonstrate the key role of essential amino acid transport in the control of mTORC1 and tumor growth caused by genetic disruption of the multifunctional CD98/LAT1 complex, Abcam EIF2AK4 antibody (Abcam, ab75836) was used in western blot on human samples at 1:500 (fig 2). Cancer Res (2016) ncbi
domestic rabbit monoclonal (EPR5970(2))
  • western blot; bovine; fig 5c
Abcam EIF2AK4 antibody (Abcam, ab134053) was used in western blot on bovine samples (fig 5c). Mol Cells (2016) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; bovine; fig 5c
Abcam EIF2AK4 antibody (Abcam, ab75836) was used in western blot on bovine samples (fig 5c). Mol Cells (2016) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; human; fig 1
Abcam EIF2AK4 antibody (Abcam, ab75836) was used in western blot on human samples (fig 1). Autophagy (2016) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; rat; fig 3
In order to determine regulation of global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha by the small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives, Abcam EIF2AK4 antibody (Abcam, 75836) was used in western blot on rat samples (fig 3). Cell Stress Chaperones (2016) ncbi
domestic rabbit monoclonal (EPR5970(2))
  • western blot; human; fig 3
Abcam EIF2AK4 antibody (Epitomics, 5417) was used in western blot on human samples (fig 3). Sci Signal (2015) ncbi
domestic rabbit monoclonal (EPR2320Y)
  • western blot; human
Abcam EIF2AK4 antibody (Abcam, ab75836) was used in western blot on human samples . Biol Open (2012) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-7)
  • western blot; human; 1:250; fig 2
In order to demonstrate the key role of essential amino acid transport in the control of mTORC1 and tumor growth caused by genetic disruption of the multifunctional CD98/LAT1 complex, Santa Cruz Biotechnology EIF2AK4 antibody (Santa-Cruz, sc-374609) was used in western blot on human samples at 1:250 (fig 2). Cancer Res (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 2b
Cell Signaling Technology EIF2AK4 antibody (Cell Signaling, 3302) was used in western blot knockout validation on mouse samples (fig 2b). PLoS Biol (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; fig 4l
Cell Signaling Technology EIF2AK4 antibody (Cell Signaling Technology, 3302) was used in western blot on human samples at 1:500 (fig 4l). elife (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s4f
Cell Signaling Technology EIF2AK4 antibody (Cell Signaling Technology, 3302) was used in western blot on mouse samples at 1:1000 (fig s4f). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:20; loading ...; fig 2
  • western blot; mouse; 1:100; loading ...; fig 1b
Cell Signaling Technology EIF2AK4 antibody (Cell Signaling, 3302) was used in immunohistochemistry - paraffin section on mouse samples at 1:20 (fig 2) and in western blot on mouse samples at 1:100 (fig 1b). Mol Reprod Dev (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 3c
Cell Signaling Technology EIF2AK4 antibody (Cell Signaling Technology, 3302) was used in western blot on human samples (fig 3c). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 2
Cell Signaling Technology EIF2AK4 antibody (Cell Signaling, 3302) was used in western blot on human samples (fig 2). Autophagy (2016) ncbi
domestic rabbit polyclonal
  • western blot; rat; fig 3
In order to determine regulation of global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha by the small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives, Cell Signaling Technology EIF2AK4 antibody (Cell signaling, 3302) was used in western blot on rat samples (fig 3). Cell Stress Chaperones (2016) ncbi
Articles Reviewed
  1. Misra J, Holmes M, T Mirek E, Langevin M, Kim H, Carlson K, et al. Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress. Nucleic Acids Res. 2021;49:5726-5742 pubmed publisher
  2. Lu Y, Kavianpour S, Zhang T, Zhang X, Nguyen D, Thombre R, et al. MARK2 phosphorylates eIF2α in response to proteotoxic stress. PLoS Biol. 2021;19:e3001096 pubmed publisher
  3. Pavlova N, King B, Josselsohn R, Violante S, Macera V, Vardhana S, et al. Translation in amino-acid-poor environments is limited by tRNAGln charging. elife. 2020;9: pubmed publisher
  4. Ruan H, Li X, Xu X, Leibowitz B, Tong J, Chen L, et al. eIF4E S209 phosphorylation licenses myc- and stress-driven oncogenesis. elife. 2020;9: pubmed publisher
  5. Mick E, Titov D, Skinner O, Sharma R, Jourdain A, Mootha V. Distinct mitochondrial defects trigger the integrated stress response depending on the metabolic state of the cell. elife. 2020;9: pubmed publisher
  6. Harding H, Ordonez A, Allen F, Parts L, Inglis A, Williams R, et al. The ribosomal P-stalk couples amino acid starvation to GCN2 activation in mammalian cells. elife. 2019;8: pubmed publisher
  7. Zhu P, Khatiwada S, Cui Y, Reineke L, Dooling S, Kim J, et al. Activation of the ISR mediates the behavioral and neurophysiological abnormalities in Down syndrome. Science. 2019;366:843-849 pubmed publisher
  8. Cao Y, Trillo Tinoco J, Sierra R, Anadon C, Dai W, Mohamed E, et al. ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression. Nat Commun. 2019;10:1280 pubmed publisher
  9. Yoon J, Park K, Hwang D, Rhee K. Importance of eIF2α phosphorylation as a protective mechanism against heat stress in mouse male germ cells. Mol Reprod Dev. 2017;84:265-274 pubmed publisher
  10. Silva R, Sattlegger E, Castilho B. Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response. J Cell Sci. 2016;129:4521-4533 pubmed
  11. Cormerais Y, Giuliano S, Lefloch R, Front B, Durivault J, Tambutte E, et al. Genetic Disruption of the Multifunctional CD98/LAT1 Complex Demonstrates the Key Role of Essential Amino Acid Transport in the Control of mTORC1 and Tumor Growth. Cancer Res. 2016;76:4481-92 pubmed publisher
  12. Chaveroux C, Sarcinelli C, Barbet V, Belfeki S, Barthelaix A, Ferraro Peyret C, et al. Nutrient shortage triggers the hexosamine biosynthetic pathway via the GCN2-ATF4 signalling pathway. Sci Rep. 2016;6:27278 pubmed publisher
  13. Xia X, Che Y, Gao Y, Zhao S, Ao C, Yang H, et al. Arginine Supplementation Recovered the IFN-?-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2? Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells. Mol Cells. 2016;39:410-7 pubmed publisher
  14. Bretin A, Carrière J, Dalmasso G, Bergougnoux A, B chir W, Maurin A, et al. Activation of the EIF2AK4-EIF2A/eIF2?-ATF4 pathway triggers autophagy response to Crohn disease-associated adherent-invasive Escherichia coli infection. Autophagy. 2016;12:770-83 pubmed publisher
  15. Hong M, Nam K, Kim K, Kim S, Kim I. The small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives regulate global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha. Cell Stress Chaperones. 2016;21:485-97 pubmed publisher
  16. Wengrod J, Wang D, Weiss S, Zhong H, Osman I, Gardner L. Phosphorylation of eIF2α triggered by mTORC1 inhibition and PP6C activation is required for autophagy and is aberrant in PP6C-mutated melanoma. Sci Signal. 2015;8:ra27 pubmed publisher
  17. Tattoli I, Philpott D, Girardin S. The bacterial and cellular determinants controlling the recruitment of mTOR to the Salmonella-containing vacuole. Biol Open. 2012;1:1215-25 pubmed publisher