This is a Validated Antibody Database (VAD) review about human EGLN3, based on 6 published articles (read how Labome selects the articles), using EGLN3 antibody in all methods. It is aimed to help Labome visitors find the most suited EGLN3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EGLN3 synonym: HIFP4H3; HIFPH3; PHD3

Knockout validation
Invitrogen
domestic rabbit polyclonal
  • western blot knockout validation; human; fig s10a
Invitrogen EGLN3 antibody (Invitrogen, PA116526) was used in western blot knockout validation on human samples (fig s10a). Nat Cell Biol (2021) ncbi
Abcam
domestic rabbit monoclonal (EPR17869)
  • western blot; human; 1:1500; loading ...; fig 3a, 6a
Abcam EGLN3 antibody (Abcam, ab184714) was used in western blot on human samples at 1:1500 (fig 3a, 6a). J Biomed Sci (2021) ncbi
domestic rabbit polyclonal
  • RNA immunoprecipitation; human; loading ...; fig 2c
  • immunohistochemistry - paraffin section; human; fig 7j
  • immunocytochemistry; human; loading ...; fig 3f
  • western blot; human; 1:1000; loading ...; fig s6d
Abcam EGLN3 antibody (Abcam, ab30782) was used in RNA immunoprecipitation on human samples (fig 2c), in immunohistochemistry - paraffin section on human samples (fig 7j), in immunocytochemistry on human samples (fig 3f) and in western blot on human samples at 1:1000 (fig s6d). Nat Commun (2021) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot knockout validation; human; fig s10a
Invitrogen EGLN3 antibody (Invitrogen, PA116526) was used in western blot knockout validation on human samples (fig s10a). Nat Cell Biol (2021) ncbi
mouse monoclonal (EG188e/d5)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2E;2D
  • western blot; mouse; 1:500; loading ...; fig 3
In order to clarify the role of Egln3 using a mouse model of low-grade glioma, Invitrogen EGLN3 antibody (Thermo Fisher Scientific, MA5-16144) was used in immunohistochemistry - paraffin section on mouse samples (fig 2E;2D) and in western blot on mouse samples at 1:500 (fig 3). Oncol Lett (2017) ncbi
Novus Biologicals
domestic rabbit polyclonal (6C5cc)
  • western blot; mouse; fig 1
Novus Biologicals EGLN3 antibody (Novus, NB100-303) was used in western blot on mouse samples (fig 1). Skelet Muscle (2016) ncbi
domestic rabbit polyclonal (6C5cc)
  • western blot; human; fig 1f
Novus Biologicals EGLN3 antibody (Novus Biologicals, NB100-303) was used in western blot on human samples (fig 1f). J Biol Chem (2016) ncbi
Articles Reviewed
  1. Chen W, Song J, Liu S, Tang B, Shen L, Zhu J, et al. USP9X promotes apoptosis in cholangiocarcinoma by modulation expression of KIF1Bβ via deubiquitinating EGLN3. J Biomed Sci. 2021;28:44 pubmed publisher
  2. Andrade J, Shi C, Costa A, Choi J, Kim J, Doddaballapur A, et al. Control of endothelial quiescence by FOXO-regulated metabolites. Nat Cell Biol. 2021;23:413-423 pubmed publisher
  3. Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, et al. The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis. Nat Commun. 2021;12:1341 pubmed publisher
  4. Mao K, You C, Lei D, Zhang H. Potential regulation of glioma through the induction of apoptosis signaling via Egl-9 family hypoxia-inducible factor 3. Oncol Lett. 2017;13:893-897 pubmed publisher
  5. Shin J, Nunomiya A, Kitajima Y, Dan T, Miyata T, Nagatomi R. Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway. Skelet Muscle. 2016;6:5 pubmed publisher
  6. Heir P, Srikumar T, Bikopoulos G, Bunda S, Poon B, Lee J, et al. Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling. J Biol Chem. 2016;291:7357-72 pubmed publisher