This is a Validated Antibody Database (VAD) review about human EGLN1, based on 8 published articles (read how Labome selects the articles), using EGLN1 antibody in all methods. It is aimed to help Labome visitors find the most suited EGLN1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EGLN1 synonym: C1orf12; ECYT3; HALAH; HIF-PH2; HIFPH2; HPH-2; HPH2; PHD2; SM20; ZMYND6

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D31E11)
  • western blot knockout validation; human; fig s10a
Cell Signaling Technology EGLN1 antibody (CST, 4835) was used in western blot knockout validation on human samples (fig s10a). Nat Cell Biol (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-8)
  • proximity ligation assay; human; 1:20; loading ...; fig 2a
Santa Cruz Biotechnology EGLN1 antibody (Santa Cruz, sc-271835) was used in proximity ligation assay on human samples at 1:20 (fig 2a). Oncotarget (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D31E11)
  • western blot knockout validation; human; fig s10a
Cell Signaling Technology EGLN1 antibody (CST, 4835) was used in western blot knockout validation on human samples (fig s10a). Nat Cell Biol (2021) ncbi
domestic rabbit monoclonal (D31E11)
  • western blot; mouse; 1:1000; loading ...; fig 2j
Cell Signaling Technology EGLN1 antibody (Cell Signaling, 4835s) was used in western blot on mouse samples at 1:1000 (fig 2j). J Clin Invest (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 5e
In order to describe a function for NQO1 in stabilizing HIF-1alpha, Cell Signaling Technology EGLN1 antibody (Cell Signaling, 3293) was used in western blot on human samples at 1:1000 (fig 5e). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 1
Cell Signaling Technology EGLN1 antibody (Cell Signaling Technology, 3293) was used in western blot on mouse samples (fig 1). Skelet Muscle (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4c
In order to discuss the contribution of LINK-A to cancer, Cell Signaling Technology EGLN1 antibody (Cell Signaling, 3293) was used in western blot on human samples at 1:1000 (fig 4c). Nat Cell Biol (2016) ncbi
domestic rabbit monoclonal (D31E11)
  • western blot; mouse; fig 3a
In order to elucidate how EglN2 regulates FOXO3a degradation, Cell Signaling Technology EGLN1 antibody (Cell Signaling, 4835) was used in western blot on mouse samples (fig 3a). Genes Dev (2014) ncbi
domestic rabbit monoclonal (D31E11)
  • western blot; mouse; 1:1000
Cell Signaling Technology EGLN1 antibody (Cell Signaling Technology, 4835) was used in western blot on mouse samples at 1:1000. J Bone Miner Res (2014) ncbi
Articles Reviewed
  1. Andrade J, Shi C, Costa A, Choi J, Kim J, Doddaballapur A, et al. Control of endothelial quiescence by FOXO-regulated metabolites. Nat Cell Biol. 2021;23:413-423 pubmed publisher
  2. Li L, Kang H, Zhang Q, D Agati V, Al Awqati Q, Lin F. FoxO3 activation in hypoxic tubules prevents chronic kidney disease. J Clin Invest. 2019;129:2374-2389 pubmed publisher
  3. Kozlova N, Wottawa M, Katschinski D, Kristiansen G, Kietzmann T. Hypoxia-inducible factor prolyl hydroxylase 2 (PHD2) is a direct regulator of epidermal growth factor receptor (EGFR) signaling in breast cancer. Oncotarget. 2017;8:9885-9898 pubmed publisher
  4. Oh E, Kim J, Kim J, Kim S, Lee J, Hong S, et al. NQO1 inhibits proteasome-mediated degradation of HIF-1α. Nat Commun. 2016;7:13593 pubmed publisher
  5. Shin J, Nunomiya A, Kitajima Y, Dan T, Miyata T, Nagatomi R. Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway. Skelet Muscle. 2016;6:5 pubmed publisher
  6. Lin A, Li C, Xing Z, Hu Q, Liang K, Han L, et al. The LINK-A lncRNA activates normoxic HIF1? signalling in triple-negative breast cancer. Nat Cell Biol. 2016;18:213-24 pubmed publisher
  7. Zheng X, Zhai B, Koivunen P, Shin S, Lu G, Liu J, et al. Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev. 2014;28:1429-44 pubmed publisher
  8. Cheng S, Xing W, Pourteymoor S, Mohan S. Conditional disruption of the prolyl hydroxylase domain-containing protein 2 (Phd2) gene defines its key role in skeletal development. J Bone Miner Res. 2014;29:2276-86 pubmed publisher