This is a Validated Antibody Database (VAD) review about human EEF1A1, based on 20 published articles (read how Labome selects the articles), using EEF1A1 antibody in all methods. It is aimed to help Labome visitors find the most suited EEF1A1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EEF1A1 synonym: CCS-3; CCS3; EE1A1; EEF-1; EEF1A; EF-Tu; EF1A; GRAF-1EF; HNGC:16303; LENG7; PTI1; eEF1A-1

Abcam
domestic rabbit monoclonal (EPR9471)
  • western blot; human; loading ...; fig 4e, 4g
Abcam EEF1A1 antibody (Abcam, ab157455) was used in western blot on human samples (fig 4e, 4g). Cell (2019) ncbi
domestic rabbit monoclonal (EPR9471)
  • western blot; mouse; 1:20,000; fig 2a
In order to find autoantigens in the skin of the affected limb using a mouse model of complex regional pain syndrome, Abcam EEF1A1 antibody (Abcam, ab157455) was used in western blot on mouse samples at 1:20,000 (fig 2a). Exp Neurol (2017) ncbi
domestic rabbit monoclonal (EPR9470)
  • immunoprecipitation; swine
  • immunocytochemistry; swine; 1:50
  • western blot; swine
Abcam EEF1A1 antibody (Abcam, ab140632) was used in immunoprecipitation on swine samples , in immunocytochemistry on swine samples at 1:50 and in western blot on swine samples . Vet Microbiol (2014) ncbi
Proteintech Group
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:100; loading ...; fig 6d
Proteintech Group EEF1A1 antibody (Proteintech, 11402-1-AP) was used in immunohistochemistry on human samples at 1:100 (fig 6d). Mol Hum Reprod (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:100; fig 7a
In order to examine the composition of mitochondrial proteins in cases of fatal familial insomnia, Cell Signaling Technology EEF1A1 antibody (Cell signaling, 2551) was used in western blot on human samples at 1:100 (fig 7a). Brain Pathol (2017) ncbi
EMD Millipore
mouse monoclonal (CBP-KK1)
  • western blot; human; loading ...; fig 6a
EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in western blot on human samples (fig 6a). Cold Spring Harb Mol Case Stud (2020) ncbi
mouse monoclonal (CBP-KK1)
  • western blot; human; 1:5000; loading ...; fig 1e, 3a, s4g
  • western blot; mouse; 1:5000; loading ...; fig 6e
EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in western blot on human samples at 1:5000 (fig 1e, 3a, s4g) and in western blot on mouse samples at 1:5000 (fig 6e). Cell (2019) ncbi
mouse monoclonal (CBP-KK1)
  • immunohistochemistry - frozen section; chicken; 1:1000; loading ...; tbl 1
  • western blot; chicken; 1:1000; loading ...; tbl 1
EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in immunohistochemistry - frozen section on chicken samples at 1:1000 (tbl 1) and in western blot on chicken samples at 1:1000 (tbl 1). J Comp Neurol (2017) ncbi
mouse monoclonal (CBP-KK1)
  • western blot; mouse; loading ...; fig 2h
EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in western blot on mouse samples (fig 2h). Cancer Res (2017) ncbi
mouse monoclonal (CBP-KK1)
  • immunocytochemistry; human; fig 8c
EMD Millipore EEF1A1 antibody (Merck-Millipore, 05-235) was used in immunocytochemistry on human samples (fig 8c). Nucleic Acids Res (2017) ncbi
mouse monoclonal (CBP-KK1)
  • western blot; Trypanosoma brucei; 1:10,000; fig 3c
In order to report that the intermembrane space domain of trypanosomal Tom22 binds mitochondrial precursor proteins and that it is essential for normal growth and mitochondrial protein import, EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in western blot on Trypanosoma brucei samples at 1:10,000 (fig 3c). Sci Rep (2017) ncbi
mouse monoclonal (CBP-KK1)
  • western blot; Trypanosoma brucei; 1:10,000; loading ...; fig 3a
In order to find non-canonical inner membrane protein translocation machinery in Trypanosoma brucei, EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in western blot on Trypanosoma brucei samples at 1:10,000 (fig 3a). Nat Commun (2016) ncbi
mouse monoclonal (CBP-KK1)
  • immunoprecipitation; mouse; loading ...; fig 5a
  • western blot; mouse; 1:2000; loading ...; fig 5a
In order to propose that GCN2 is an important sensor of the state of the actin cytoskeleton, EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in immunoprecipitation on mouse samples (fig 5a) and in western blot on mouse samples at 1:2000 (fig 5a). J Cell Sci (2016) ncbi
mouse monoclonal (CBP-KK1)
  • western blot; human; loading ...; fig 3c
In order to elucidate glucocorticoid receptor-mediated mRNA decay, EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in western blot on human samples (fig 3c). Genes Dev (2016) ncbi
mouse monoclonal (CBP-KK1)
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 2
In order to study interplay in autophagy regulation between transglutaminase type 2 and pyruvate kinase isoenzyme M2, EMD Millipore EEF1A1 antibody (Milipore, 05-235) was used in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 2). Oncotarget (2015) ncbi
mouse monoclonal (CBP-KK1)
  • western blot; human; fig 1a
EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in western blot on human samples (fig 1a). Sci Rep (2015) ncbi
mouse monoclonal (CBP-KK1)
  • western blot; Trypanosoma brucei; 1:10,000; fig 1
In order to research kinetoplastids, mitochondrial protein import receptors, to reveal convergent evolution over vast phylogenetic distances, EMD Millipore EEF1A1 antibody (Merck Millipore, 05-235) was used in western blot on Trypanosoma brucei samples at 1:10,000 (fig 1). Nat Commun (2015) ncbi
mouse monoclonal (CBP-KK1)
  • western blot; mouse; 1:2000
EMD Millipore EEF1A1 antibody (EMD Millipore, 05-235) was used in western blot on mouse samples at 1:2000. Addict Biol (2016) ncbi
mouse monoclonal (CBP-KK1)
  • immunoprecipitation; mouse
EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in immunoprecipitation on mouse samples . J Biol Chem (2012) ncbi
mouse monoclonal (CBP-KK1)
  • western blot; swine; fig 4
EMD Millipore EEF1A1 antibody (Millipore, 05-235) was used in western blot on swine samples (fig 4). Mol Cell Proteomics (2011) ncbi
Articles Reviewed
  1. Ricker C, Berlow N, Crawford K, Georgopapadakos T, Huelskamp A, Woods A, et al. Undifferentiated small round cell sarcoma in a young male: a case report. Cold Spring Harb Mol Case Stud. 2020;6: pubmed publisher
  2. Jeppesen D, Fenix A, Franklin J, Higginbotham J, Zhang Q, Zimmerman L, et al. Reassessment of Exosome Composition. Cell. 2019;177:428-445.e18 pubmed publisher
  3. Liu S, Hausmann S, CARLSON S, Fuentes M, Francis J, Pillai R, et al. METTL13 Methylation of eEF1A Increases Translational Output to Promote Tumorigenesis. Cell. 2019;176:491-504.e21 pubmed publisher
  4. Sakano H, Zorio D, Wang X, Ting Y, Noble W, MacCoss M, et al. Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein. J Comp Neurol. 2017;525:3341-3359 pubmed publisher
  5. Akiel M, Guo C, Li X, Rajasekaran D, Mendoza R, Robertson C, et al. IGFBP7 Deletion Promotes Hepatocellular Carcinoma. Cancer Res. 2017;77:4014-4025 pubmed publisher
  6. Malecki J, Aileni V, Ho A, Schwarz J, Moen A, Sørensen V, et al. The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A). Nucleic Acids Res. 2017;45:4370-4389 pubmed publisher
  7. Mani J, Rout S, Desy S, Schneider A. Mitochondrial protein import - Functional analysis of the highly diverged Tom22 orthologue of Trypanosoma brucei. Sci Rep. 2017;7:40738 pubmed publisher
  8. Neuhaus N, Yoon J, Terwort N, Kliesch S, Seggewiss J, Huge A, et al. Single-cell gene expression analysis reveals diversity among human spermatogonia. Mol Hum Reprod. 2017;23:79-90 pubmed publisher
  9. Harsman A, Oeljeklaus S, Wenger C, Huot J, Warscheid B, Schneider A. The non-canonical mitochondrial inner membrane presequence translocase of trypanosomatids contains two essential rhomboid-like proteins. Nat Commun. 2016;7:13707 pubmed publisher
  10. Silva R, Sattlegger E, Castilho B. Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response. J Cell Sci. 2016;129:4521-4533 pubmed
  11. Park O, Park J, Yu M, An H, Ko J, Kim Y. Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay. Genes Dev. 2016;30:2093-2105 pubmed
  12. Tajerian M, Hung V, Khan H, Lahey L, Sun Y, Birklein F, et al. Identification of KRT16 as a target of an autoantibody response in complex regional pain syndrome. Exp Neurol. 2017;287:14-20 pubmed publisher
  13. Frau Méndez M, Fernández Vega I, Ansoleaga B, Blanco Tech R, Carmona Tech M, Antonio Del Río J, et al. Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus. Brain Pathol. 2017;27:95-106 pubmed publisher
  14. Altuntas S, Rossin F, Marsella C, D Eletto M, Diaz Hidalgo L, Farrace M, et al. The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation. Oncotarget. 2015;6:44941-54 pubmed publisher
  15. Chiu K, Kuo T, Kuok Q, Lin Y, Hua C, Lin C, et al. ADAM9 enhances CDCP1 protein expression by suppressing miR-218 for lung tumor metastasis. Sci Rep. 2015;5:16426 pubmed publisher
  16. Mani J, Desy S, Niemann M, Chanfon A, Oeljeklaus S, Pusnik M, et al. Mitochondrial protein import receptors in Kinetoplastids reveal convergent evolution over large phylogenetic distances. Nat Commun. 2015;6:6646 pubmed publisher
  17. Uys J, McGuier N, Gass J, Griffin W, Ball L, Mulholland P. Chronic intermittent ethanol exposure and withdrawal leads to adaptations in nucleus accumbens core postsynaptic density proteome and dendritic spines. Addict Biol. 2016;21:560-74 pubmed publisher
  18. Zhang X, Shi H, Chen J, Shi D, Li C, Feng L. EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication. Vet Microbiol. 2014;172:443-8 pubmed publisher
  19. Saikia M, Krokowski D, Guan B, Ivanov P, Parisien M, Hu G, et al. Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress. J Biol Chem. 2012;287:42708-25 pubmed publisher
  20. Yun Hong Y, Chih Fan C, Chia Wei C, Yen Chung C. A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum. Mol Cell Proteomics. 2011;10:M110.007138 pubmed publisher