This is a Validated Antibody Database (VAD) review about human E-selectin, based on 25 published articles (read how Labome selects the articles), using E-selectin antibody in all methods. It is aimed to help Labome visitors find the most suited E-selectin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
E-selectin synonym: CD62E; ELAM; ELAM1; ESEL; LECAM2; E-selectin; CD62 antigen-like family member E; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2

Invitrogen
mouse monoclonal (1.2B6)
  • other; human; loading ...; fig 2b
In order to optimize the collection and analysis of extracellular vesicles, Invitrogen E-selectin antibody (Thermo Scientific, MA1-22165) was used in other on human samples (fig 2b). J Immunol Methods (2016) ncbi
mouse monoclonal (P2H3)
  • blocking or activating experiments; pig
In order to discuss and investigate methods of cellular glycoengineering, Invitrogen E-selectin antibody (eBioscience, P2H3) was used in blocking or activating experiments on pig samples . Biomaterials (2016) ncbi
mouse monoclonal (1.2B6)
  • ELISA; human; fig 2
In order to characterize capabilities and potentials of extracellular vesicle (EV) array, Invitrogen E-selectin antibody (Thermo Fisher Scientific, MA1-22165) was used in ELISA on human samples (fig 2). J Extracell Vesicles (2015) ncbi
mouse monoclonal (P2H3)
  • flow cytometry; human; fig 5
Invitrogen E-selectin antibody (eBioscience, P2H3) was used in flow cytometry on human samples (fig 5). J Tissue Eng Regen Med (2015) ncbi
R&D Systems
mouse monoclonal (BBIG-E4)
  • western blot; human; 1:1000; fig 6g
R&D Systems E-selectin antibody (R&D systems, BBIG-E4) was used in western blot on human samples at 1:1000 (fig 6g). Nat Commun (2015) ncbi
mouse monoclonal (BBIG-E6 (13D5))
  • other; human; fig 2
In order to characterize capabilities and potentials of extracellular vesicle (EV) array, R&D Systems E-selectin antibody (R&D Systems, BBA1) was used in other on human samples (fig 2). J Extracell Vesicles (2015) ncbi
mouse monoclonal (BBIG-E5)
  • flow cytometry; baboons
R&D Systems E-selectin antibody (R&D Systems, BBIG-E5) was used in flow cytometry on baboons samples . Am J Cardiovasc Dis (2014) ncbi
mouse monoclonal (BBIG-E4)
  • western blot; human; loading ...; fig 3c
R&D Systems E-selectin antibody (R&D Systems, BBA16) was used in western blot on human samples (fig 3c). J Cell Physiol (2015) ncbi
mouse monoclonal (BBIG-E4)
  • blocking or activating experiments; human; fig 6
R&D Systems E-selectin antibody (R&D, BBA16) was used in blocking or activating experiments on human samples (fig 6). Toxicol In Vitro (2014) ncbi
mouse monoclonal (BBIG-E4)
  • western blot; human
R&D Systems E-selectin antibody (R&D systems, BBA16) was used in western blot on human samples . Cardiovasc Res (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (CTB202)
  • immunocytochemistry; human; 1:50; loading ...; fig 4b
Santa Cruz Biotechnology E-selectin antibody (Santa Cruz, sc-5262) was used in immunocytochemistry on human samples at 1:50 (fig 4b). J Lipid Res (2018) ncbi
mouse monoclonal (A-10)
  • western blot; human
Santa Cruz Biotechnology E-selectin antibody (Santa Cruz, sc-137203) was used in western blot on human samples . PLoS ONE (2014) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4a
Abcam E-selectin antibody (Abcam, ab18981) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 4a). Nat Commun (2018) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 9a
In order to study the involvement of SENP1-mediated GATA2 deSUMOylation in graft arteriosclerosis, Abcam E-selectin antibody (Abcam, ab18981) was used in western blot on human samples at 1:1000 (fig 9a). Nat Commun (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 8d
  • western blot; mouse; loading ...; fig 8c
Abcam E-selectin antibody (Abcam, ab18981) was used in immunohistochemistry on mouse samples (fig 8d) and in western blot on mouse samples (fig 8c). J Am Heart Assoc (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 1
Abcam E-selectin antibody (Abcam, ab18981) was used in western blot on human samples (fig 1). Sci Rep (2016) ncbi
LifeSpan Biosciences
goat polyclonal
  • immunohistochemistry - paraffin section; human
  • immunohistochemistry - paraffin section; rat
In order to examine the adverse effect of prostaglandin F-receptor antagonist AS604872 on brain vasculature, LifeSpan Biosciences E-selectin antibody (Lifespan Biosciences, LS-C150295) was used in immunohistochemistry - paraffin section on human samples and in immunohistochemistry - paraffin section on rat samples . J Pharmacol Sci (2014) ncbi
BioLegend
mouse monoclonal (HAE-1f)
  • flow cytometry; human; fig 4
In order to determine the differential regulation of the inflammatory phenotype of brain microvascular endothelial cells by pro-inflammatory TNFalpha and IL-1beta, BioLegend E-selectin antibody (Biolegend, 336011) was used in flow cytometry on human samples (fig 4). J Neuroinflammation (2015) ncbi
Bio-Rad
mouse monoclonal (CL2/6)
  • flow cytometry; human
Bio-Rad E-selectin antibody (Serotec, MCA1969F) was used in flow cytometry on human samples . Br J Pharmacol (2014) ncbi
MyBioSource
MyBioSource E-selectin antibody (MyBioSource, MBS343017) was used . J Inflamm (Lond) (2015) ncbi
BD Biosciences
mouse monoclonal (68-5H11)
  • flow cytometry; human; 1:100; loading ...; fig 2e
In order to study the interactions among different monocyte populations and vascular endothelial cells, BD Biosciences E-selectin antibody (BD Biosciences, 68-5H11) was used in flow cytometry on human samples at 1:100 (fig 2e). J Immunol (2017) ncbi
mouse monoclonal (68-5H11)
  • flow cytometry; human; 1:500; fig 5
In order to investigate the compromise of brain endothelial barriers and promotion of cerebral malaria pathogenesis by plasmodium falciparum histidine-rich protein II, BD Biosciences E-selectin antibody (BD Biosciences, 555648) was used in flow cytometry on human samples at 1:500 (fig 5). MBio (2016) ncbi
mouse monoclonal (68-5H11)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences E-selectin antibody (BD, 551145) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
EMD Millipore
mouse monoclonal (P2H3)
  • ELISA; human; 1:50; loading ...; fig 4a
EMD Millipore E-selectin antibody (Millipore, P2H3) was used in ELISA on human samples at 1:50 (fig 4a). Inflammation (2015) ncbi
mouse monoclonal (P2H3)
  • ELISA; human; 1:50; loading ...; fig 3
EMD Millipore E-selectin antibody (Millipore, P2H3) was used in ELISA on human samples at 1:50 (fig 3). Cell Signal (2014) ncbi
mouse monoclonal (P2H3)
  • immunocytochemistry; human; 1:50; loading ...; fig 3a
EMD Millipore E-selectin antibody (Millipore, P2H3) was used in immunocytochemistry on human samples at 1:50 (fig 3a). Vascul Pharmacol (2014) ncbi
Articles Reviewed
  1. García Nores G, Ly C, Cuzzone D, Kataru R, Hespe G, Torrisi J, et al. CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema. Nat Commun. 2018;9:1970 pubmed publisher
  2. Hartman C, Duerr M, Albert C, Neumann W, McHowat J, Ford D. 2-Chlorofatty acids induce Weibel-Palade body mobilization. J Lipid Res. 2018;59:113-122 pubmed publisher
  3. Qiu C, Wang Y, Zhao H, Qin L, Shi Y, Zhu X, et al. The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis. Nat Commun. 2017;8:15426 pubmed publisher
  4. Chimen M, Yates C, McGettrick H, Ward L, Harrison M, Apta B, et al. Monocyte Subsets Coregulate Inflammatory Responses by Integrated Signaling through TNF and IL-6 at the Endothelial Cell Interface. J Immunol. 2017;198:2834-2843 pubmed publisher
  5. Du M, Wang X, Tan X, Li X, Huang D, Huang K, et al. Nkx2-5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E-Deficient Mice. J Am Heart Assoc. 2016;5: pubmed
  6. Liu Z, Tian R, Li Y, Zhang L, Shao H, Yang C, et al. SDF-1?-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia. Sci Rep. 2016;6:34416 pubmed publisher
  7. Bæk R, Søndergaard E, Varming K, Jørgensen M. The impact of various preanalytical treatments on the phenotype of small extracellular vesicles in blood analyzed by protein microarray. J Immunol Methods. 2016;438:11-20 pubmed publisher
  8. Pal P, Daniels B, Oskman A, Diamond M, Klein R, Goldberg D. Plasmodium falciparum Histidine-Rich Protein II Compromises Brain Endothelial Barriers and May Promote Cerebral Malaria Pathogenesis. MBio. 2016;7: pubmed publisher
  9. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  10. Roth Flach R, Skoura A, Matevossian A, Danai L, Zheng W, Cortes C, et al. Endothelial protein kinase MAP4K4 promotes vascular inflammation and atherosclerosis. Nat Commun. 2015;6:8995 pubmed publisher
  11. Lo C, Weil B, Palka B, Momeni A, Canty J, Neelamegham S. Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model. Biomaterials. 2016;74:19-30 pubmed publisher
  12. O Carroll S, Kho D, Wiltshire R, Nelson V, Rotimi O, Johnson R, et al. Pro-inflammatory TNFα and IL-1β differentially regulate the inflammatory phenotype of brain microvascular endothelial cells. J Neuroinflammation. 2015;12:131 pubmed publisher
  13. Chang C, Wu S, Lin C, Kwan A. Valproic acid attenuates intercellular adhesion molecule-1 and E-selectin through a chemokine ligand 5 dependent mechanism and subarachnoid hemorrhage induced vasospasm in a rat model. J Inflamm (Lond). 2015;12:27 pubmed publisher
  14. Jørgensen M, Bæk R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles. 2015;4:26048 pubmed publisher
  15. Shi Q, Hodara V, Meng Q, Voruganti V, Rice K, Michalek J, et al. Early endothelial damage detected by circulating particles in baboons fed a diet high in simple carbohydrates in conjunction with saturated or unsaturated fat. Am J Cardiovasc Dis. 2014;4:123-32 pubmed
  16. Fukuda M, Aoki T, Manabe T, Maekawa A, Shirakawa T, Kataoka H, et al. Exacerbation of intracranial aneurysm and aortic dissection in hypertensive rat treated with the prostaglandin F-receptor antagonist AS604872. J Pharmacol Sci. 2014;126:230-42 pubmed
  17. Lee W, Ku S, Bae J. Anti-inflammatory effects of Baicalin, Baicalein, and Wogonin in vitro and in vivo. Inflammation. 2015;38:110-25 pubmed publisher
  18. Shikama Y, Aki N, Hata A, Nishimura M, Oyadomari S, Funaki M. Palmitate-stimulated monocytes induce adhesion molecule expression in endothelial cells via IL-1 signaling pathway. J Cell Physiol. 2015;230:732-42 pubmed publisher
  19. Jumpertz S, Hennes T, Asare Y, Vervoorts J, Bernhagen J, Schütz A. The ?-catenin E3 ubiquitin ligase SIAH-1 is regulated by CSN5/JAB1 in CRC cells. Cell Signal. 2014;26:2051-9 pubmed publisher
  20. Lee W, Ku S, Bae J. Vascular barrier protective effects of orientin and isoorientin in LPS-induced inflammation in vitro and in vivo. Vascul Pharmacol. 2014;62:3-14 pubmed publisher
  21. Poussin C, Gallitz I, Schlage W, Steffen Y, Stolle K, Lebrun S, et al. Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in an in vitro assay revealed by transcriptomics analysis. Toxicol In Vitro. 2014;28:896-908 pubmed publisher
  22. Hamada K, Osaka M, Yoshida M. Cell density impacts epigenetic regulation of cytokine-induced E-selectin gene expression in vascular endothelium. PLoS ONE. 2014;9:e90502 pubmed publisher
  23. Fakhrudin N, Waltenberger B, Cabaravdic M, Atanasov A, Malainer C, Schachner D, et al. Identification of plumericin as a potent new inhibitor of the NF-?B pathway with anti-inflammatory activity in vitro and in vivo. Br J Pharmacol. 2014;171:1676-86 pubmed publisher
  24. Fessner A, Esser J, Bluhm F, Grundmann S, Zhou Q, Patterson C, et al. The transcription factor HoxB5 stimulates vascular remodelling in a cytokine-dependent manner. Cardiovasc Res. 2014;101:247-55 pubmed publisher
  25. Denecke B, Horsch L, Radtke S, Fischer J, Horn P, Giebel B. Human endothelial colony-forming cells expanded with an improved protocol are a useful endothelial cell source for scaffold-based tissue engineering. J Tissue Eng Regen Med. 2015;9:E84-97 pubmed publisher