This is a Validated Antibody Database (VAD) review about human DUX4, based on 6 published articles (read how Labome selects the articles), using DUX4 antibody in all methods. It is aimed to help Labome visitors find the most suited DUX4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DUX4 synonym: DUX4L

Abcam
domestic rabbit monoclonal (E5-5)
  • western blot; human; loading ...; fig 2e
Abcam DUX4 antibody (Abcam, ab124699) was used in western blot on human samples (fig 2e). Sci Adv (2019) ncbi
domestic rabbit monoclonal (E5-5)
  • chromatin immunoprecipitation; human; loading ...; fig 7d
  • immunocytochemistry; human; 1:250; loading ...; fig 9e
  • western blot; human; loading ...; fig s7
In order to identify sex-related factors that regulate the severity of facioscapulohumeral muscular dystrophy, Abcam DUX4 antibody (Abcam, 124699) was used in chromatin immunoprecipitation on human samples (fig 7d), in immunocytochemistry on human samples at 1:250 (fig 9e) and in western blot on human samples (fig s7). J Clin Invest (2017) ncbi
domestic rabbit monoclonal (E5-5)
  • western blot; human; loading ...; fig 1b
Abcam DUX4 antibody (Abcam, ab124699) was used in western blot on human samples (fig 1b). Nucleic Acids Res (2016) ncbi
Invitrogen
mouse monoclonal (P4H2)
  • immunohistochemistry - paraffin section; human; 1:200; fig 2B
In order to use DUX4 immunostaining to distinguish CIC-DUX4 tumors from other round cell mimics, Invitrogen DUX4 antibody (Thermo Fisher Scientific, MA5-16147) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 2B). Am J Surg Pathol (2017) ncbi
EMD Millipore
mouse monoclonal (9A12)
  • immunocytochemistry; human; 1:4000; loading ...; fig s3d
  • western blot; human; 1:1000; loading ...; fig 2f
EMD Millipore DUX4 antibody (Millipore, 9A12) was used in immunocytochemistry on human samples at 1:4000 (fig s3d) and in western blot on human samples at 1:1000 (fig 2f). Nat Microbiol (2019) ncbi
mouse monoclonal (9A12)
  • western blot; mouse; 1:1000; fig 8h
In order to identify Ret as a downstream mediator of DUX4 signaling, EMD Millipore DUX4 antibody (Millipore, 9A12) was used in western blot on mouse samples at 1:1000 (fig 8h). elife (2016) ncbi
Articles Reviewed
  1. Desimone A, Leszyk J, Wagner K, Emerson C. Identification of the hyaluronic acid pathway as a therapeutic target for facioscapulohumeral muscular dystrophy. Sci Adv. 2019;5:eaaw7099 pubmed publisher
  2. Full F, van Gent M, Sparrer K, Chiang C, Zurenski M, Scherer M, et al. Centrosomal protein TRIM43 restricts herpesvirus infection by regulating nuclear lamina integrity. Nat Microbiol. 2019;4:164-176 pubmed publisher
  3. Teveroni E, Pellegrino M, Sacconi S, Calandra P, Cascino I, Farioli Vecchioli S, et al. Estrogens enhance myoblast differentiation in facioscapulohumeral muscular dystrophy by antagonizing DUX4 activity. J Clin Invest. 2017;127:1531-1545 pubmed publisher
  4. Siegele B, Roberts J, Black J, Rudzinski E, Vargas S, Galambos C. DUX4 Immunohistochemistry Is a Highly Sensitive and Specific Marker for CIC-DUX4 Fusion-positive Round Cell Tumor. Am J Surg Pathol. 2017;41:423-429 pubmed publisher
  5. Moyle L, Blanc E, Jaka O, Prueller J, Banerji C, Tedesco F, et al. Ret function in muscle stem cells points to tyrosine kinase inhibitor therapy for facioscapulohumeral muscular dystrophy. elife. 2016;5: pubmed publisher
  6. Choi S, Gearhart M, Cui Z, Bosnakovski D, Kim M, Schennum N, et al. DUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes. Nucleic Acids Res. 2016;44:5161-73 pubmed publisher