This is a Validated Antibody Database (VAD) review about human DSG2, based on 13 published articles (read how Labome selects the articles), using DSG2 antibody in all methods. It is aimed to help Labome visitors find the most suited DSG2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DSG2 synonym: CDHF5; HDGC

Knockout validation
Invitrogen
mouse monoclonal (6D8)
  • western blot knockout validation; human; 1:500; loading ...; fig 1b
Invitrogen DSG2 antibody (ThermoFisher, 32-6100) was used in western blot knockout validation on human samples at 1:500 (fig 1b). Sci Rep (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (6D8)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 2a
  • western blot knockout validation; mouse; loading ...; fig 2a
In order to report that SB216763 prevents myocyte injury and cardiac dysfunction in two murine models of arrhythmogenic cardiomyopathy, Santa Cruz Biotechnology DSG2 antibody (Santa Cruz, sc-53486) was used in immunohistochemistry knockout validation on mouse samples (fig 2a) and in western blot knockout validation on mouse samples (fig 2a). JCI Insight (2016) ncbi
Invitrogen
mouse monoclonal (6D8)
  • western blot knockout validation; human; 1:500; loading ...; fig 1b
Invitrogen DSG2 antibody (ThermoFisher, 32-6100) was used in western blot knockout validation on human samples at 1:500 (fig 1b). Sci Rep (2021) ncbi
mouse monoclonal (6D8)
  • western blot; human; loading ...; fig 7a
In order to study how CUB domain-containing protein1 contributes to metastasis and drug resistance, Invitrogen DSG2 antibody (Invitrogen, 32-6100) was used in western blot on human samples (fig 7a). Breast Cancer Res (2016) ncbi
mouse monoclonal (6D8)
  • immunohistochemistry - paraffin section; human; fig 5b
  • flow cytometry; human; loading ...; fig 2c
In order to study the function of desmoglein-2 and generate knockout mice, Invitrogen DSG2 antibody (Invitrogen, 6D8) was used in immunohistochemistry - paraffin section on human samples (fig 5b) and in flow cytometry on human samples (fig 2c). Angiogenesis (2016) ncbi
mouse monoclonal (6D8)
  • immunocytochemistry; human
  • western blot; human
In order to determine the role of desmoglein 2 in melanoma cells, Invitrogen DSG2 antibody (Invitrogen, clone 6D8) was used in immunocytochemistry on human samples and in western blot on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (6D8)
  • immunohistochemistry; human; 1:10
In order to examine the contribution of the desmosomal compartment to gastroesophageal reflux disease, Invitrogen DSG2 antibody (Zymed, 326100) was used in immunohistochemistry on human samples at 1:10. Hum Pathol (2012) ncbi
mouse monoclonal (6D8)
  • western blot; human; fig 3
In order to investigate the effect of EGF on the expression of E-cadherin and desmoglein and its mechanism, Invitrogen DSG2 antibody (Invitrogen, 6D8) was used in western blot on human samples (fig 3). Dermatol Res Pract (2012) ncbi
mouse monoclonal (6D8)
  • immunohistochemistry; human; 1:10; fig 1
In order to examine the role of desmosomal proteins in gastro-oesophageal reflux disease, Invitrogen DSG2 antibody (Zymed, 326100) was used in immunohistochemistry on human samples at 1:10 (fig 1). Histopathology (2012) ncbi
Abcam
domestic rabbit monoclonal (EPR6768)
  • immunocytochemistry; human; 1:200; loading ...; fig s4c
  • immunohistochemistry - paraffin section; mouse; 1:1500; loading ...; fig s3e
Abcam DSG2 antibody (Abcam, ab150372) was used in immunocytochemistry on human samples at 1:200 (fig s4c) and in immunohistochemistry - paraffin section on mouse samples at 1:1500 (fig s3e). Sci Rep (2021) ncbi
domestic rabbit monoclonal (EPR6768)
  • immunocytochemistry; mouse; 1:250; loading ...; fig 1d
Abcam DSG2 antibody (Abcam, ab150372) was used in immunocytochemistry on mouse samples at 1:250 (fig 1d). J Cell Sci (2019) ncbi
Bio-Rad
mouse monoclonal (6D8)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 2a
Bio-Rad DSG2 antibody (BioRad, MCA2272T) was used in immunohistochemistry on mouse samples at 1:100 (fig 2a). PLoS Pathog (2021) ncbi
mouse monoclonal (6D8)
  • ELISA; human
Bio-Rad DSG2 antibody (Serotec, MCA2272) was used in ELISA on human samples . J Virol (2012) ncbi
Santa Cruz Biotechnology
mouse monoclonal (6D8)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 2a
  • western blot knockout validation; mouse; loading ...; fig 2a
In order to report that SB216763 prevents myocyte injury and cardiac dysfunction in two murine models of arrhythmogenic cardiomyopathy, Santa Cruz Biotechnology DSG2 antibody (Santa Cruz, sc-53486) was used in immunohistochemistry knockout validation on mouse samples (fig 2a) and in western blot knockout validation on mouse samples (fig 2a). JCI Insight (2016) ncbi
mouse monoclonal (AH12.2)
  • immunocytochemistry; human
Santa Cruz Biotechnology DSG2 antibody (Santa Cruz Biotechnology, clone AH12.2) was used in immunocytochemistry on human samples . Cancer Res (2015) ncbi
Articles Reviewed
  1. Shelton W, Thomas S, Alexander H, Thomes C, Conway D, Dubash A. Desmoglein-2 harnesses a PDZ-GEF2/Rap1 signaling axis to control cell spreading and focal adhesions independent of cell-cell adhesion. Sci Rep. 2021;11:13295 pubmed publisher
  2. Evstatiev R, Cervenka A, Austerlitz T, Deim G, Baumgartner M, Beer A, et al. The food additive EDTA aggravates colitis and colon carcinogenesis in mouse models. Sci Rep. 2021;11:5188 pubmed publisher
  3. McLellan L, McAllaster M, Kim A, Tothova L, Olson P, Pinkner J, et al. A host receptor enables type 1 pilus-mediated pathogenesis of Escherichia coli pyelonephritis. PLoS Pathog. 2021;17:e1009314 pubmed publisher
  4. Li Y, Merkel C, Zeng X, Heier J, Cantrell P, Sun M, et al. The N-cadherin interactome in primary cardiomyocytes as defined using quantitative proximity proteomics. J Cell Sci. 2019;132: pubmed publisher
  5. LAW M, Ferreira R, Davis B, Higgins P, Kim J, Castellano R, et al. CUB domain-containing protein 1 and the epidermal growth factor receptor cooperate to induce cell detachment. Breast Cancer Res. 2016;18:80 pubmed publisher
  6. Ebert L, Tan L, Johan M, Min K, Cockshell M, Parham K, et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis. Angiogenesis. 2016;19:463-86 pubmed publisher
  7. Chelko S, Asimaki A, Andersen P, Bedja D, Amat Alarcon N, Demazumder D, et al. Central role for GSK3β in the pathogenesis of arrhythmogenic cardiomyopathy. JCI Insight. 2016;1: pubmed publisher
  8. Saias L, Gomes A, Cazales M, Ducommun B, Lobjois V. Cell-Cell Adhesion and Cytoskeleton Tension Oppose Each Other in Regulating Tumor Cell Aggregation. Cancer Res. 2015;75:2426-33 pubmed publisher
  9. Peitsch W, Doerflinger Y, Fischer Colbrie R, Huck V, Bauer A, Utikal J, et al. Desmoglein 2 depletion leads to increased migration and upregulation of the chemoattractant secretoneurin in melanoma cells. PLoS ONE. 2014;9:e89491 pubmed publisher
  10. Wex T, Kuester D, Monkemuller K, Stahr A, Fry L, Kandulski A, et al. Assessment of desmosomal components (desmoglein 1-3, plakoglobin) in cardia mucosa in relation to gastroesophageal reflux disease and Helicobacter pylori infection. Hum Pathol. 2012;43:1745-54 pubmed publisher
  11. Wang H, Beyer I, Persson J, Song H, Li Z, Richter M, et al. A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses. J Virol. 2012;86:6286-302 pubmed publisher
  12. Chavez M, Buhr C, Petrie W, Wandinger Ness A, Kusewitt D, Hudson L. Differential downregulation of e-cadherin and desmoglein by epidermal growth factor. Dermatol Res Pract. 2012;2012:309587 pubmed publisher
  13. Wex T, Monkemuller K, Stahr A, Kuester D, Fry L, Völkel S, et al. Gastro-oesophageal reflux disease is associated with up-regulation of desmosomal components in oesophageal mucosa. Histopathology. 2012;60:405-15 pubmed publisher