This is a Validated Antibody Database (VAD) review about human DROSHA, based on 18 published articles (read how Labome selects the articles), using DROSHA antibody in all methods. It is aimed to help Labome visitors find the most suited DROSHA antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DROSHA synonym: ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D28B1)
  • western blot knockout validation; mouse; loading ...; fig s3d
In order to study the function of DGCR8 in murine embryonic stem cells, Cell Signaling Technology DROSHA antibody (Cell Signaling, 3364) was used in western blot knockout validation on mouse samples (fig s3d). J Cell Biol (2017) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4b
Abcam DROSHA antibody (Abcam, ab12286) was used in western blot on human samples (fig 4b). Nat Commun (2019) ncbi
domestic rabbit monoclonal (EPR12794)
  • western blot; human; loading ...; fig s3a
Abcam DROSHA antibody (Abcam, ab183732) was used in western blot on human samples (fig s3a). Cell (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig e5a
Abcam DROSHA antibody (Abcam, ab12286) was used in western blot on human samples (fig e5a). Nature (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:250; fig 4
  • western blot; human; 1:1000; fig 2
In order to study the affects in the subcellular localization of Drosha by alternative splicing, Abcam DROSHA antibody (Abcam, ab-12286) was used in immunocytochemistry on human samples at 1:250 (fig 4) and in western blot on human samples at 1:1000 (fig 2). Nucleic Acids Res (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000
Abcam DROSHA antibody (Abcam, ab12286) was used in western blot on human samples at 1:1000. Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 3c
  • immunocytochemistry; human; loading ...; fig 3f
  • western blot; human; loading ...; fig 3b
Abcam DROSHA antibody (Abcam, ab12286) was used in chromatin immunoprecipitation on human samples (fig 3c), in immunocytochemistry on human samples (fig 3f) and in western blot on human samples (fig 3b). Oncogene (2016) ncbi
Novus Biologicals
domestic rabbit polyclonal (RL2)
In order to propose that DGCR8 helps to recruit the exosome complex to RNAs and induce their degradation, Novus Biologicals DROSHA antibody (Novus, NBP1-03349) was used . Mol Cell (2015) ncbi
domestic rabbit polyclonal (RL2)
Novus Biologicals DROSHA antibody (Novus Biologicals, NBP1-03349) was used . Leukemia (2015) ncbi
Invitrogen
domestic rabbit monoclonal (S.12.0)
  • western blot; human; loading ...; fig s3c
Invitrogen DROSHA antibody (Thermo Fisher, MA5-14784) was used in western blot on human samples (fig s3c). Nucleic Acids Res (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • western blot; human; loading ...; fig 6a
Santa Cruz Biotechnology DROSHA antibody (Santa Cruz, sc-393591) was used in western blot on human samples (fig 6a). Sci Rep (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D28B1)
  • western blot; human; loading ...; fig 3b
Cell Signaling Technology DROSHA antibody (Cell Signaling, 3364S) was used in western blot on human samples (fig 3b). Cell (2019) ncbi
domestic rabbit monoclonal (D28B1)
  • chromatin immunoprecipitation; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 1b
Cell Signaling Technology DROSHA antibody (CST, 3364) was used in chromatin immunoprecipitation on human samples (fig 2a) and in western blot on human samples (fig 1b). Biochim Biophys Acta Gene Regul Mech (2017) ncbi
domestic rabbit monoclonal (D28B1)
  • western blot knockout validation; mouse; loading ...; fig s3d
In order to study the function of DGCR8 in murine embryonic stem cells, Cell Signaling Technology DROSHA antibody (Cell Signaling, 3364) was used in western blot knockout validation on mouse samples (fig s3d). J Cell Biol (2017) ncbi
domestic rabbit monoclonal (D28B1)
  • western blot; human; fig s3b
Cell Signaling Technology DROSHA antibody (Cell Signaling, 3364) was used in western blot on human samples (fig s3b). Nucleic Acids Res (2016) ncbi
domestic rabbit monoclonal (D28B1)
  • western blot; human; 1:500; fig 3
In order to study the affects in the subcellular localization of Drosha by alternative splicing, Cell Signaling Technology DROSHA antibody (Cell Signaling, 3364) was used in western blot on human samples at 1:500 (fig 3). Nucleic Acids Res (2016) ncbi
domestic rabbit monoclonal (D28B1)
  • western blot; mouse; 1:1000; fig 2
Cell Signaling Technology DROSHA antibody (Cell Signaling Tech, 3364) was used in western blot on mouse samples at 1:1000 (fig 2). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D28B1)
  • western blot; human; 1:1000; fig s1
In order to suggest that that DICER, DROSHA, and DDRNAs recruit DNA damage factors, Cell Signaling Technology DROSHA antibody (Cell Signaling, 3364) was used in western blot on human samples at 1:1000 (fig s1). J Cell Sci (2016) ncbi
domestic rabbit monoclonal (D28B1)
  • western blot; human; 1:1000; fig 4
Cell Signaling Technology DROSHA antibody (Cell Signaling, 3364) was used in western blot on human samples at 1:1000 (fig 4). Nucleic Acids Res (2015) ncbi
domestic rabbit monoclonal (D28B1)
  • western blot; human; 1:1000
Cell Signaling Technology DROSHA antibody (Cell Signaling, 3364) was used in western blot on human samples at 1:1000. Cell (2014) ncbi
domestic rabbit monoclonal (D30F3)
  • western blot; human; 1:1000
Cell Signaling Technology DROSHA antibody (Cell Signaling, 3410) was used in western blot on human samples at 1:1000. Cell (2014) ncbi
domestic rabbit monoclonal (D28B1)
  • western blot; mouse; fig 5
Cell Signaling Technology DROSHA antibody (Cell Signaling, 3364) was used in western blot on mouse samples (fig 5). J Biol Chem (2014) ncbi
Articles Reviewed
  1. Zhang J, Bai R, Li M, Ye H, Wu C, Wang C, et al. Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression. Nat Commun. 2019;10:1858 pubmed publisher
  2. Jeppesen D, Fenix A, Franklin J, Higginbotham J, Zhang Q, Zimmerman L, et al. Reassessment of Exosome Composition. Cell. 2019;177:428-445.e18 pubmed publisher
  3. Bonath F, Domingo Prim J, Tarbier M, Friedländer M, Visa N. Next-generation sequencing reveals two populations of damage-induced small RNAs at endogenous DNA double-strand breaks. Nucleic Acids Res. 2018;46:11869-11882 pubmed publisher
  4. Takata M, Gonçalves Carneiro D, Zang T, Soll S, York A, Blanco Melo D, et al. CG dinucleotide suppression enables antiviral defence targeting non-self RNA. Nature. 2017;550:124-127 pubmed publisher
  5. Patne K, Rakesh R, Arya V, Chanana U, Sethy R, Swer P, et al. BRG1 and SMARCAL1 transcriptionally co-regulate DROSHA, DGCR8 and DICER in response to doxorubicin-induced DNA damage. Biochim Biophys Acta Gene Regul Mech. 2017;1860:936-951 pubmed publisher
  6. Dattilo V, D Antona L, Talarico C, Capula M, Catalogna G, Iuliano R, et al. SGK1 affects RAN/RANBP1/RANGAP1 via SP1 to play a critical role in pre-miRNA nuclear export: a new route of epigenomic regulation. Sci Rep. 2017;7:45361 pubmed publisher
  7. Cirera Salinas D, Yu J, Bodak M, Ngondo R, Herbert K, Ciaudo C. Noncanonical function of DGCR8 controls mESC exit from pluripotency. J Cell Biol. 2017;216:355-366 pubmed publisher
  8. Warner M, Bridge K, Hewitson J, Hodgkinson M, Heyam A, Massa B, et al. S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells. Nucleic Acids Res. 2016;44:9942-9955 pubmed
  9. Link S, Grund S, Diederichs S. Alternative splicing affects the subcellular localization of Drosha. Nucleic Acids Res. 2016;44:5330-43 pubmed publisher
  10. Salzman D, Nakamura K, Nallur S, Dookwah M, Metheetrairut C, Slack F, et al. miR-34 activity is modulated through 5'-end phosphorylation in response to DNA damage. Nat Commun. 2016;7:10954 pubmed publisher
  11. Garibaldi F, Falcone E, Trisciuoglio D, Colombo T, Lisek K, Walerych D, et al. Mutant p53 inhibits miRNA biogenesis by interfering with the microprocessor complex. Oncogene. 2016;35:3760-70 pubmed publisher
  12. Murai K, Sun G, Ye P, Tian E, Yang S, Cui Q, et al. The TLX-miR-219 cascade regulates neural stem cell proliferation in neurodevelopment and schizophrenia iPSC model. Nat Commun. 2016;7:10965 pubmed publisher
  13. Francia S, Cabrini M, Matti V, Oldani A, d Adda di Fagagna F. DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors. J Cell Sci. 2016;129:1468-76 pubmed publisher
  14. Macias S, Cordiner R, Gautier P, Plass M, Cáceres J. DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs. Mol Cell. 2015;60:873-85 pubmed publisher
  15. Zhu C, Chen C, Huang J, Zhang H, Zhao X, Deng R, et al. SUMOylation at K707 of DGCR8 controls direct function of primary microRNA. Nucleic Acids Res. 2015;43:7945-60 pubmed publisher
  16. Veronese A, Pepe F, Chiacchia J, Pagotto S, Lanuti P, Veschi S, et al. Allele-specific loss and transcription of the miR-15a/16-1 cluster in chronic lymphocytic leukemia. Leukemia. 2015;29:86-95 pubmed publisher
  17. Mori M, Triboulet R, Mohseni M, Schlegelmilch K, Shrestha K, Camargo F, et al. Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer. Cell. 2014;156:893-906 pubmed publisher
  18. Gurung B, Muhammad A, Hua X. Menin is required for optimal processing of the microRNA let-7a. J Biol Chem. 2014;289:9902-8 pubmed publisher