This is a Validated Antibody Database (VAD) review about human DNMT3b, based on 24 published articles (read how Labome selects the articles), using DNMT3b antibody in all methods. It is aimed to help Labome visitors find the most suited DNMT3b antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DNMT3b synonym: ICF; ICF1; M.HsaIIIB

Knockout validation
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:250; loading ...; fig s1f
Abcam DNMT3b antibody (Abcam, ab2851) was used in western blot knockout validation on human samples at 1:250 (fig s1f). PLoS Pathog (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig s1c
Santa Cruz Biotechnology DNMT3b antibody (Santa Cruz, sc-393845) was used in western blot knockout validation on mouse samples at 1:1000 (fig s1c). Biomolecules (2021) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 2f
Invitrogen DNMT3b antibody (Thermo Fisher, PA1-884) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2f). EBioMedicine (2021) ncbi
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:250; loading ...; fig s1f
Abcam DNMT3b antibody (Abcam, ab2851) was used in western blot knockout validation on human samples at 1:250 (fig s1f). PLoS Pathog (2021) ncbi
domestic rabbit polyclonal
  • RNA immunoprecipitation; human; 1:100; loading ...; fig 4e
  • chromatin immunoprecipitation; human; 1:100; loading ...; fig 4g
Abcam DNMT3b antibody (Abcam, ab2851) was used in RNA immunoprecipitation on human samples at 1:100 (fig 4e) and in chromatin immunoprecipitation on human samples at 1:100 (fig 4g). Aging (Albany NY) (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 4a
  • western blot; human; 1:1000; loading ...; fig 2f
Abcam DNMT3b antibody (Abcam, ab2851) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 4a) and in western blot on human samples at 1:1000 (fig 2f). J Exp Clin Cancer Res (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4d
Abcam DNMT3b antibody (Abcam, ab2851) was used in western blot on human samples (fig 4d). Onco Targets Ther (2019) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 6d
  • western blot; mouse; 1:2000; loading ...; fig 6a
  • immunoprecipitation; human; loading ...; fig 3g
  • western blot; human; 1:2000; loading ...; fig 3b
Abcam DNMT3b antibody (Abcam, ab2851) was used in chromatin immunoprecipitation on mouse samples (fig 6d), in western blot on mouse samples at 1:2000 (fig 6a), in immunoprecipitation on human samples (fig 3g) and in western blot on human samples at 1:2000 (fig 3b). Nucleic Acids Res (2017) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; fig 4
  • western blot; mouse; fig 4
Abcam DNMT3b antibody (Abcam, ab2851) was used in chromatin immunoprecipitation on mouse samples (fig 4) and in western blot on mouse samples (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig s1
Abcam DNMT3b antibody (abcam, ab2851) was used in western blot on human samples at 1:1000 (fig s1). Oncotarget (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig s1c
Santa Cruz Biotechnology DNMT3b antibody (Santa Cruz, sc-393845) was used in western blot knockout validation on mouse samples at 1:1000 (fig s1c). Biomolecules (2021) ncbi
mouse monoclonal (4H84)
  • western blot; mouse; 1:800; fig 2
Santa Cruz Biotechnology DNMT3b antibody (Santa Cruz, sc-70984) was used in western blot on mouse samples at 1:800 (fig 2). Biomed Res Int (2015) ncbi
mouse monoclonal (2280C3a)
  • western blot; human; 1:1000; fig 2
Santa Cruz Biotechnology DNMT3b antibody (Santa Cruz, sc-81252) was used in western blot on human samples at 1:1000 (fig 2). PLoS ONE (2015) ncbi
mouse monoclonal (52A1018)
  • western blot; human
Santa Cruz Biotechnology DNMT3b antibody (Santa Cruz Biotechnology, sc-52922) was used in western blot on human samples . Biochem Biophys Res Commun (2014) ncbi
mouse monoclonal (2280C3a)
  • western blot; human
Santa Cruz Biotechnology DNMT3b antibody (Santa Cruz, sc-81252) was used in western blot on human samples . PLoS ONE (2013) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 2f
Invitrogen DNMT3b antibody (Thermo Fisher, PA1-884) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2f). EBioMedicine (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 1i
Invitrogen DNMT3b antibody (Thermo Fisher, PA1-884) was used in western blot on mouse samples at 1:1000 (fig 1i). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 6
In order to determine if the capacity for de novo DNA methylation of developmentally regulated loci is preserved in differentiated somatic cells, Invitrogen DNMT3b antibody (ThermoFisher Scientific, AFPA1884) was used in western blot on mouse samples (fig 6). Nucleic Acids Res (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 4
In order to characterize the G9a/GLP complex and how it maintains imprinted DNA methylation in embryonic stem cells, Invitrogen DNMT3b antibody (Thermo Scientific, PA1-884) was used in western blot on human samples (fig 4). Cell Rep (2016) ncbi
Active Motif
mouse monoclonal (52A1018)
  • western blot; mouse; 1:1000
Active Motif DNMT3b antibody (Active Motif, 39207) was used in western blot on mouse samples at 1:1000. Mech Ageing Dev (2013) ncbi
mouse monoclonal (52A1018)
  • chromatin immunoprecipitation; human
Active Motif DNMT3b antibody (Active motif, 39207) was used in chromatin immunoprecipitation on human samples . Biochem Pharmacol (2011) ncbi
MyBioSource
mouse monoclonal (2280C3a)
  • western blot; human; 1:100; fig 3
MyBioSource DNMT3b antibody (MyBioSource, MBS120171) was used in western blot on human samples at 1:100 (fig 3). Genes Chromosomes Cancer (2011) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • western blot; mouse; fig 4a
Novus Biologicals DNMT3b antibody (Novus Biologicals, NB300-516) was used in western blot on mouse samples (fig 4a). Sci Rep (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D7O7O)
  • western blot; human; loading ...; fig 3d
Cell Signaling Technology DNMT3b antibody (Cell Signaling Technology, 67259) was used in western blot on human samples (fig 3d). Int J Med Sci (2021) ncbi
domestic rabbit monoclonal (D7O7O)
  • immunocytochemistry; human; 1:1000; loading ...; fig 2g
  • western blot; human; 1:5000; loading ...; fig 2f
Cell Signaling Technology DNMT3b antibody (CST, 67259) was used in immunocytochemistry on human samples at 1:1000 (fig 2g) and in western blot on human samples at 1:5000 (fig 2f). Sci Adv (2020) ncbi
domestic rabbit monoclonal (D7O7O)
  • immunocytochemistry; human; loading ...; fig s7b
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology DNMT3b antibody (Cell Signaling, D7O7O) was used in immunocytochemistry on human samples (fig s7b) and in western blot on human samples (fig 2c). J Virol (2019) ncbi
domestic rabbit monoclonal (D7O7O)
  • western blot; human; fig 6d
In order to understand the role of nicotinamide metabolism in the maintenance of glioblastoma stem cells, Cell Signaling Technology DNMT3b antibody (Cell signaling, 67259) was used in western blot on human samples (fig 6d). JCI Insight (2017) ncbi
Articles Reviewed
  1. Han J, Chen X, Xu J, Chu L, Li R, Sun N, et al. Simultaneous silencing Aurora-A and UHRF1 inhibits colorectal cancer cell growth through regulating expression of DNMT1 and STAT1. Int J Med Sci. 2021;18:3437-3451 pubmed publisher
  2. Wang S, Cao Q, Cui X, Jing J, Li F, Shi H, et al. Dnmt3b Deficiency in Myf5+-Brown Fat Precursor Cells Promotes Obesity in Female Mice. Biomolecules. 2021;11: pubmed publisher
  3. Qin W, Brands X, Van T Veer C, F de Vos A, Sirard J, J T H Roelofs J, et al. Bronchial epithelial DNA methyltransferase 3b dampens pulmonary immune responses during Pseudomonas aeruginosa infection. PLoS Pathog. 2021;17:e1009491 pubmed publisher
  4. Shao G, Fan X, Zhang P, Liu X, Huang L, Ji S. Methylation-dependent MCM6 repression induced by LINC00472 inhibits triple-negative breast cancer metastasis by disturbing the MEK/ERK signaling pathway. Aging (Albany NY). 2021;13:4962-4975 pubmed publisher
  5. Lin Z, Huang W, He Q, Li D, Wang Z, Feng Y, et al. FOXC1 promotes HCC proliferation and metastasis by Upregulating DNMT3B to induce DNA Hypermethylation of CTH promoter. J Exp Clin Cancer Res. 2021;40:50 pubmed publisher
  6. Lopušná K, Nowialis P, Opavska J, Abraham A, Riva A, Opavsky R. Dnmt3b catalytic activity is critical for its tumour suppressor function in lymphomagenesis and is associated with c-Met oncogenic signalling. EBioMedicine. 2021;63:103191 pubmed publisher
  7. Kim K, Wu Y, Yoon J, Adachi K, Wu G, Velychko S, et al. Reprogramming competence of OCT factors is determined by transactivation domains. Sci Adv. 2020;6: pubmed publisher
  8. Nowialis P, Lopušná K, Opavska J, Haney S, Abraham A, Sheng P, et al. Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions. Nat Commun. 2019;10:4374 pubmed publisher
  9. Wu R, Yun Q, Zhang J, Bao J. Downregulation of KLF13 through DNMT1-mediated hypermethylation promotes glioma cell proliferation and invasion. Onco Targets Ther. 2019;12:1509-1520 pubmed publisher
  10. Liu S, Liu L, Xu G, Cao Z, Wang Q, Li S, et al. Epigenetic Modification Is Regulated by the Interaction of Influenza A Virus Nonstructural Protein 1 with the De Novo DNA Methyltransferase DNMT3B and Subsequent Transport to the Cytoplasm for K48-Linked Polyubiquitination. J Virol. 2019;93: pubmed publisher
  11. Jung J, Kim L, Wang X, Wu Q, Sanvoranart T, Hubert C, et al. Nicotinamide metabolism regulates glioblastoma stem cell maintenance. JCI Insight. 2017;2: pubmed publisher
  12. Raciti G, Spinelli R, Desiderio A, Longo M, Parrillo L, Nigro C, et al. Specific CpG hyper-methylation leads to Ankrd26 gene down-regulation in white adipose tissue of a mouse model of diet-induced obesity. Sci Rep. 2017;7:43526 pubmed publisher
  13. Chen S, Jing Y, Kang X, Yang L, Wang D, Zhang W, et al. Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy. Nucleic Acids Res. 2017;45:1144-1158 pubmed publisher
  14. Termanis A, Torrea N, Culley J, Kerr A, Ramsahoye B, Stancheva I. The SNF2 family ATPase LSH promotes cell-autonomous de novo DNA methylation in somatic cells. Nucleic Acids Res. 2016;44:7592-604 pubmed publisher
  15. Zhang T, Termanis A, Özkan B, Bao X, Culley J, de Lima Alves F, et al. G9a/GLP Complex Maintains Imprinted DNA Methylation in Embryonic Stem Cells. Cell Rep. 2016;15:77-85 pubmed publisher
  16. Su R, Strug M, Jeong J, Miele L, Fazleabas A. Aberrant activation of canonical Notch1 signaling in the mouse uterus decreases progesterone receptor by hypermethylation and leads to infertility. Proc Natl Acad Sci U S A. 2016;113:2300-5 pubmed publisher
  17. Song G, Shi L, Guo Y, Yu L, Wang L, Zhang X, et al. A novel PAD4/SOX4/PU.1 signaling pathway is involved in the committed differentiation of acute promyelocytic leukemia cells into granulocytic cells. Oncotarget. 2016;7:3144-57 pubmed publisher
  18. Gasque Schoof C, Izzotti A, Jasiulionis M, Vasques L. The Roles of miR-26, miR-29, and miR-203 in the Silencing of the Epigenetic Machinery during Melanocyte Transformation. Biomed Res Int. 2015;2015:634749 pubmed publisher
  19. Nakamura K, Nakabayashi K, Htet Aung K, Aizawa K, Hori N, Yamauchi J, et al. DNA methyltransferase inhibitor zebularine induces human cholangiocarcinoma cell death through alteration of DNA methylation status. PLoS ONE. 2015;10:e0120545 pubmed publisher
  20. Huang H, Hu G, Cai J, Xia B, Liu J, Li X, et al. Role of poly(ADP-ribose) glycohydrolase silencing in DNA hypomethylation induced by benzo(a)pyrene. Biochem Biophys Res Commun. 2014;452:708-14 pubmed publisher
  21. Takasugi M, Hayakawa K, Arai D, Shiota K. Age- and sex-dependent DNA hypomethylation controlled by growth hormone in mouse liver. Mech Ageing Dev. 2013;134:331-7 pubmed publisher
  22. Nakamura K, Aizawa K, Nakabayashi K, Kato N, Yamauchi J, Hata K, et al. DNA methyltransferase inhibitor zebularine inhibits human hepatic carcinoma cells proliferation and induces apoptosis. PLoS ONE. 2013;8:e54036 pubmed publisher
  23. Simo Riudalbas L, Melo S, Esteller M. DNMT3B gene amplification predicts resistance to DNA demethylating drugs. Genes Chromosomes Cancer. 2011;50:527-34 pubmed publisher
  24. Karius T, Schnekenburger M, Ghelfi J, Walter J, Dicato M, Diederich M. Reversible epigenetic fingerprint-mediated glutathione-S-transferase P1 gene silencing in human leukemia cell lines. Biochem Pharmacol. 2011;81:1329-42 pubmed publisher