This is a Validated Antibody Database (VAD) review about human DNAJB6, based on 5 published articles (read how Labome selects the articles), using DNAJB6 antibody in all methods. It is aimed to help Labome visitors find the most suited DNAJB6 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DNAJB6 synonym: DJ4; DnaJ; HHDJ1; HSJ-2; HSJ2; LGMD1D; LGMD1E; MRJ; MSJ-1; dnaJ homolog subfamily B member 6; DnaJ (Hsp40) homolog, subfamily B, member 6; DnaJ-like 2 protein; heat shock protein J2

Knockout validation
Abcam
rabbit monoclonal (EPR17122)
  • western blot knockout validation; human; 1:2000; loading ...; fig 1a
  • immunocytochemistry; human; loading ...; fig 1b
In order to study the suppressive effect of DNAJB6 and Hsp70 on alpha-synuclein aggregation, Abcam DNAJB6 antibody (Abcam, ab198995) was used in western blot knockout validation on human samples at 1:2000 (fig 1a) and in immunocytochemistry on human samples (fig 1b). Sci Rep (2017) ncbi
Abnova
mouse monoclonal (2C11-C1)
  • western blot; human; loading ...; fig 8d
Abnova DNAJB6 antibody (Cedarlane Laboratories, H00010049-M01) was used in western blot on human samples (fig 8d). Nucleic Acids Res (2017) ncbi
mouse monoclonal (2C11-C1)
  • immunocytochemistry; human; 1:300; loading ...; fig 3c
  • western blot; human; 1:1000; loading ...; fig 1a
In order to determine the capacity of the short isoform of DNAJB6 to protect against 1-methyl-4phenylpridinium ion-induced apoptosis in LN18 cells as a model for Parkinson's disease, Abnova DNAJB6 antibody (Abnova, H00010049-M01) was used in immunocytochemistry on human samples at 1:300 (fig 3c) and in western blot on human samples at 1:1000 (fig 1a). Oxid Med Cell Longev (2017) ncbi
Abcam
rabbit monoclonal (EPR17122)
  • western blot knockout validation; human; 1:2000; loading ...; fig 1a
  • immunocytochemistry; human; loading ...; fig 1b
In order to study the suppressive effect of DNAJB6 and Hsp70 on alpha-synuclein aggregation, Abcam DNAJB6 antibody (Abcam, ab198995) was used in western blot knockout validation on human samples at 1:2000 (fig 1a) and in immunocytochemistry on human samples (fig 1b). Sci Rep (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-5)
  • immunohistochemistry; rat; fig 1b
  • western blot; rat; fig 4b
Santa Cruz Biotechnology DNAJB6 antibody (Santa Cruz, B-5) was used in immunohistochemistry on rat samples (fig 1b) and in western blot on rat samples (fig 4b). Mol Neurobiol (2016) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; 1:250; fig 4
In order to report that the DNAJB6 mutation, Pro96Arg, caused a distal-onset myopathy in one family, Invitrogen DNAJB6 antibody (Thermo Fisher, PA5-21401) was used in western blot on human samples at 1:250 (fig 4). Acta Neuropathol Commun (2015) ncbi
Articles Reviewed
  1. Aprile F, Källstig E, Limorenko G, Vendruscolo M, Ron D, Hansen C. The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress ?-synuclein aggregation. Sci Rep. 2017;7:9039 pubmed publisher
  2. Tien J, Mazloomian A, Cheng S, Hughes C, Chow C, Canapi L, et al. CDK12 regulates alternative last exon mRNA splicing and promotes breast cancer cell invasion. Nucleic Acids Res. 2017;45:6698-6716 pubmed publisher
  3. Hong Y, Hong Y, Choi Y, Yeo S, Jin S, Lee S, et al. The Short Isoform of DNAJB6 Protects against 1-Methyl-4-phenylpridinium Ion-Induced Apoptosis in LN18 Cells via Inhibiting Both ROS Formation and Mitochondrial Membrane Potential Loss. Oxid Med Cell Longev. 2017;2017:7982389 pubmed publisher
  4. Smith C, D Mello S. Cell and Context-Dependent Effects of the Heat Shock Protein DNAJB6 on Neuronal Survival. Mol Neurobiol. 2016;53:5628-39 pubmed publisher
  5. Ruggieri A, Brancati F, Zanotti S, Maggi L, Pasanisi M, Saredi S, et al. Complete loss of the DNAJB6 G/F domain and novel missense mutations cause distal-onset DNAJB6 myopathy. Acta Neuropathol Commun. 2015;3:44 pubmed publisher