This is a Validated Antibody Database (VAD) review about human DNA-PKcs, based on 38 published articles (read how Labome selects the articles), using DNA-PKcs antibody in all methods. It is aimed to help Labome visitors find the most suited DNA-PKcs antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DNA-PKcs synonym: DNA-PKC; DNA-PKcs; DNAPK; DNAPKc; DNPK1; HYRC; HYRC1; IMD26; XRCC7; p350

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal
  • western blot knockout validation; human; loading ...; fig 5a
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, G12) was used in western blot knockout validation on human samples (fig 5a). elife (2020) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-12)
  • western blot knockout validation; human; loading ...; fig 5a
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, G12) was used in western blot knockout validation on human samples (fig 5a). elife (2020) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 6b
Cell Signaling Technology DNA-PKcs antibody (Cell Signaling, 4602) was used in western blot knockout validation on human samples at 1:1000 (fig 6b). Nat Commun (2016) ncbi
Abcam
domestic rabbit monoclonal (Y393)
  • western blot; human; 1:1000; loading ...; fig 1f
Abcam DNA-PKcs antibody (Abcam, ab32566) was used in western blot on human samples at 1:1000 (fig 1f). J Exp Clin Cancer Res (2021) ncbi
domestic rabbit monoclonal (EPR5670)
  • western blot; human; 1:1000; loading ...; fig 1f
Abcam DNA-PKcs antibody (Abcam, ab174576) was used in western blot on human samples at 1:1000 (fig 1f). J Exp Clin Cancer Res (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 4s2a
  • western blot; human; loading ...; fig 4g, 6b
Abcam DNA-PKcs antibody (Abcam, ab18192) was used in immunocytochemistry on human samples (fig 4s2a) and in western blot on human samples (fig 4g, 6b). elife (2020) ncbi
mouse monoclonal (18-2)
  • western blot; human; 1:500-1:2000; loading ...; fig 5c, 6e
Abcam DNA-PKcs antibody (Abcam, ab44815) was used in western blot on human samples at 1:500-1:2000 (fig 5c, 6e). Cell Rep (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3e
Abcam DNA-PKcs antibody (Abcam, ab70250) was used in western blot on human samples at 1:1000 (fig 3e). Cell Rep (2018) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; fig 6k
  • western blot; mouse; 1:100; loading ...; fig 6l
  • immunocytochemistry; human; loading ...; fig 2h, 4j
  • western blot; human; 1:100; loading ...; fig 2i, 4b, 6n
Abcam DNA-PKcs antibody (Abcam, ab18192) was used in immunocytochemistry on mouse samples (fig 6k), in western blot on mouse samples at 1:100 (fig 6l), in immunocytochemistry on human samples (fig 2h, 4j) and in western blot on human samples at 1:100 (fig 2i, 4b, 6n). Nucleic Acids Res (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 3d
Abcam DNA-PKcs antibody (Abcam, ab18192) was used in western blot on human samples (fig 3d). Nucleic Acids Res (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig s2
In order to describe a role for NIPBL in DNA damage repair, Abcam DNA-PKcs antibody (Abcam, ab18192) was used in western blot on human samples at 1:1000 (fig s2). J Cell Sci (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:500; loading ...; fig 6c
In order to describe a role for APRIN in homologous recombination, Abcam DNA-PKcs antibody (ABCAM, ab18192) was used in immunocytochemistry on human samples at 1:500 (fig 6c). Nucleic Acids Res (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 6c
In order to use knockout mice to determine if GRK6 contributes to hematopoiesis, Abcam DNA-PKcs antibody (Abcam, ab18192) was used in western blot on human samples (fig 6c). Cell Death Dis (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 6c
In order to use knockout mice to determine if GRK6 contributes to hematopoiesis, Abcam DNA-PKcs antibody (Abcam, ab70250) was used in western blot on human samples (fig 6c). Cell Death Dis (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 5C
In order to investigate the role of the PTEN phosphatase in DNA interstrand crosslink repair, Abcam DNA-PKcs antibody (Abcam, ab18192) was used in immunocytochemistry on human samples (fig 5C). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 6e
In order to identify genetic features regulating cell survival after exposure to radiation., Abcam DNA-PKcs antibody (Abcam, 18192) was used in western blot on human samples at 1:1000 (fig 6e). Nat Commun (2016) ncbi
domestic rabbit monoclonal (Y393)
  • western blot; human; loading ...; fig 4c
In order to study the effect of cathepsin L on glioma stem cells both in vivo and in vitro, Abcam DNA-PKcs antibody (Abcam, ab32566) was used in western blot on human samples (fig 4c). Cancer Lett (2016) ncbi
domestic rabbit monoclonal (EPR5670)
  • western blot; human; loading ...; fig 4c
In order to study the effect of cathepsin L on glioma stem cells both in vivo and in vitro, Abcam DNA-PKcs antibody (Abcam, ab124918) was used in western blot on human samples (fig 4c). Cancer Lett (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:400; fig s6
Abcam DNA-PKcs antibody (Abcam, ab18192) was used in western blot on human samples at 1:400 (fig s6). Nat Genet (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 4
Abcam DNA-PKcs antibody (Abcam, ab18192) was used in western blot on human samples (fig 4). Nucleic Acids Res (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 2
In order to study how the Fanconi anemia pathway promotes homologous recombination at stalled replication forks, Abcam DNA-PKcs antibody (Abcam, ab18192) was used in immunocytochemistry on human samples (fig 2). Nucleic Acids Res (2016) ncbi
domestic rabbit monoclonal (Y393)
  • western blot; human; 1:1000; fig 1g
In order to elucidate the function of TRIM29 in double stranded break repair, Abcam DNA-PKcs antibody (Abcam, ab32566) was used in western blot on human samples at 1:1000 (fig 1g). Nat Commun (2015) ncbi
domestic rabbit monoclonal (EPR5670)
  • western blot; human; fig 1
Abcam DNA-PKcs antibody (Abcam, ab124918) was used in western blot on human samples (fig 1). Viruses (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (6)
  • immunocytochemistry; human; 1:200; loading ...; fig 2d
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, sc-135886) was used in immunocytochemistry on human samples at 1:200 (fig 2d). Nat Commun (2021) ncbi
mouse monoclonal (G-12)
  • western blot knockout validation; human; loading ...; fig 5a
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, G12) was used in western blot knockout validation on human samples (fig 5a). elife (2020) ncbi
mouse monoclonal
  • western blot knockout validation; human; loading ...; fig 5a
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, G12) was used in western blot knockout validation on human samples (fig 5a). elife (2020) ncbi
mouse monoclonal (G-12)
  • western blot; human; loading ...; fig 1b
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, sc-390849) was used in western blot on human samples (fig 1b). elife (2020) ncbi
mouse monoclonal (E-6)
  • immunocytochemistry; human; loading ...; fig 6i
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, sc390698) was used in immunocytochemistry on human samples (fig 6i). J Cell Physiol (2017) ncbi
mouse monoclonal (E-3)
  • western blot; human; 1:500; loading ...; fig s6b
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, sc-390495) was used in western blot on human samples at 1:500 (fig s6b). PLoS ONE (2016) ncbi
mouse monoclonal (G-4)
  • western blot; human; fig 4
In order to study the involvement of TIMELESS in the PARP1-mediated DNA damage response, Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, sc-5282) was used in western blot on human samples (fig 4). Cell Rep (2015) ncbi
mouse monoclonal (G-4)
  • western blot; mouse; 1:3000; fig 4
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, sc-5282) was used in western blot on mouse samples at 1:3000 (fig 4). Nat Commun (2015) ncbi
mouse monoclonal (6)
  • western blot; human; 1:300; fig 2
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz, sc-135886) was used in western blot on human samples at 1:300 (fig 2). J Invest Dermatol (2015) ncbi
mouse monoclonal (E-6)
  • immunocytochemistry; human; fig 4
Santa Cruz Biotechnology DNA-PKcs antibody (SantaCruz, sc390698) was used in immunocytochemistry on human samples (fig 4). Oncotarget (2015) ncbi
mouse monoclonal (G-4)
  • immunocytochemistry; African green monkey; fig 4c
  • western blot; African green monkey; fig 4a
Santa Cruz Biotechnology DNA-PKcs antibody (Santa, sc-5282) was used in immunocytochemistry on African green monkey samples (fig 4c) and in western blot on African green monkey samples (fig 4a). PLoS Pathog (2014) ncbi
mouse monoclonal (G-4)
  • immunohistochemistry; human; 1:50
Santa Cruz Biotechnology DNA-PKcs antibody (Santa Cruz Biotech, sc-5282) was used in immunohistochemistry on human samples at 1:50. Biochem Biophys Res Commun (2014) ncbi
mouse monoclonal (G-4)
  • western blot; human; 1:1000; loading ...; fig 4a
Santa Cruz Biotechnology DNA-PKcs antibody (SantaCruz, sc-5282) was used in western blot on human samples at 1:1000 (fig 4a). Cancer Med (2014) ncbi
Invitrogen
mouse monoclonal (3H6)
  • immunocytochemistry; human; fig 4
In order to study the response to double-stranded breaks at the nucleolar organizer regions, Invitrogen DNA-PKcs antibody (Thermo Scientific, MA5-15813) was used in immunocytochemistry on human samples (fig 4). Genes Dev (2015) ncbi
mouse monoclonal (18-2)
  • western blot; human; 1:200; fig 4
In order to determine how PAXX interacts with Ku to promote DNA double-strand break repair, Invitrogen DNA-PKcs antibody (Thermo Scientific, MS-369-P1) was used in western blot on human samples at 1:200 (fig 4). Science (2015) ncbi
mouse monoclonal (18-2)
  • western blot; human; 1:200; tbl 2
In order to develop a new method to visualize DNA double-strand break complexes, Invitrogen DNA-PKcs antibody (Thermo Fisher, 18.2) was used in western blot on human samples at 1:200 (tbl 2). J Cell Biol (2013) ncbi
Novus Biologicals
mouse monoclonal (3H6)
  • western blot; human; loading ...; fig 2a
Novus Biologicals DNA-PKcs antibody (Novus Biologicals, NBP2-22128) was used in western blot on human samples (fig 2a). Mol Biol Cell (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3d, e9a
Cell Signaling Technology DNA-PKcs antibody (Cell Signalling, 4602) was used in western blot on human samples (fig 3d, e9a). Nature (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 6i
Cell Signaling Technology DNA-PKcs antibody (Cell Signaling, 4602S) was used in western blot on human samples (fig 6i). Mol Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; fig s2
Cell Signaling Technology DNA-PKcs antibody (Cell signaling, 4602) was used in western blot on human samples (fig s2). Nucleic Acids Res (2016) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 6b
Cell Signaling Technology DNA-PKcs antibody (Cell Signaling, 4602) was used in western blot knockout validation on human samples at 1:1000 (fig 6b). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3a
In order to study quinacrine-induced apoptosis, Cell Signaling Technology DNA-PKcs antibody (Cell Signaling, 4602) was used in western blot on human samples (fig 3a). Biochem Pharmacol (2016) ncbi
mouse monoclonal (3H6)
  • western blot; human; fig 4
In order to study the involvement of TIMELESS in the PARP1-mediated DNA damage response, Cell Signaling Technology DNA-PKcs antibody (Cell Signalling, 12311) was used in western blot on human samples (fig 4). Cell Rep (2015) ncbi
mouse monoclonal (3H6)
  • western blot; human; fig 5
Cell Signaling Technology DNA-PKcs antibody (Cell Signaling, 12311) was used in western blot on human samples (fig 5). PLoS ONE (2015) ncbi
Articles Reviewed
  1. Lei X, Cao K, Chen Y, Shen H, Liu Z, Qin H, et al. Nuclear Transglutaminase 2 interacts with topoisomerase II⍺ to promote DNA damage repair in lung cancer cells. J Exp Clin Cancer Res. 2021;40:224 pubmed publisher
  2. Nam J, Kim A, Choi S, Kim J, Choi K, Cho S, et al. An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage. Nat Commun. 2021;12:3279 pubmed publisher
  3. Brunner A, Suryo Rahmanto A, Johansson H, Franco M, Viiliäinen J, Gazi M, et al. PTEN and DNA-PK determine sensitivity and recovery in response to WEE1 inhibition in human breast cancer. elife. 2020;9: pubmed publisher
  4. Zhu S, Paydar M, Wang F, Li Y, Wang L, Barrette B, et al. Kinesin Kif2C in regulation of DNA double strand break dynamics and repair. elife. 2020;9: pubmed publisher
  5. Wall C, Rose C, Adrian M, Zeng Y, Kirkpatrick D, Bingol B. PPEF2 Opposes PINK1-Mediated Mitochondrial Quality Control by Dephosphorylating Ubiquitin. Cell Rep. 2019;29:3280-3292.e7 pubmed publisher
  6. Nassour J, Radford R, Correia A, Fusté J, Schoell B, Jauch A, et al. Autophagic cell death restricts chromosomal instability during replicative crisis. Nature. 2019;565:659-663 pubmed publisher
  7. Tay L, Krishnan V, Sankar H, Chong Y, Chuang L, Tan T, et al. RUNX Poly(ADP-Ribosyl)ation and BLM Interaction Facilitate the Fanconi Anemia Pathway of DNA Repair. Cell Rep. 2018;24:1747-1755 pubmed publisher
  8. Kannan A, Bhatia K, Branzei D, Gangwani L. Combined deficiency of Senataxin and DNA-PKcs causes DNA damage accumulation and neurodegeneration in spinal muscular atrophy. Nucleic Acids Res. 2018;46:8326-8346 pubmed publisher
  9. Li M, Yang X, LU X, Dai N, Zhang S, Cheng Y, et al. APE1 deficiency promotes cellular senescence and premature aging features. Nucleic Acids Res. 2018;46:5664-5677 pubmed publisher
  10. Huang T, Fowler F, Chen C, Shen Z, SLECKMAN B, Tyler J. The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells. Mol Cell. 2018;69:879-892.e5 pubmed publisher
  11. Bot C, Pfeiffer A, Giordano F, Manjeera D, Dantuma N, Ström L. Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage. J Cell Sci. 2017;130:1134-1146 pubmed publisher
  12. Squillaro T, Antonucci I, Alessio N, Esposito A, Cipollaro M, Melone M, et al. Impact of lysosomal storage disorders on biology of mesenchymal stem cells: Evidences from in vitro silencing of glucocerebrosidase (GBA) and alpha-galactosidase A (GLA) enzymes. J Cell Physiol. 2017;232:3454-3467 pubmed publisher
  13. Fantini D, Huang S, Asara J, Bagchi S, Raychaudhuri P. Chromatin association of XRCC5/6 in the absence of DNA damage depends on the XPE gene product DDB2. Mol Biol Cell. 2017;28:192-200 pubmed publisher
  14. Couturier A, Fleury H, Patenaude A, Bentley V, Rodrigue A, Coulombe Y, et al. Roles for APRIN (PDS5B) in homologous recombination and in ovarian cancer prediction. Nucleic Acids Res. 2016;44:10879-10897 pubmed
  15. Zhang H, Sun L, Wang K, Wu D, Trappio M, Witting C, et al. Loss of H3K9me3 Correlates with ATM Activation and Histone H2AX Phosphorylation Deficiencies in Hutchinson-Gilford Progeria Syndrome. PLoS ONE. 2016;11:e0167454 pubmed publisher
  16. Le Q, Yao W, Chen Y, Yan B, Liu C, Yuan M, et al. GRK6 regulates ROS response and maintains hematopoietic stem cell self-renewal. Cell Death Dis. 2016;7:e2478 pubmed publisher
  17. Vuono E, Mukherjee A, Vierra D, Adroved M, Hodson C, Deans A, et al. The PTEN phosphatase functions cooperatively with the Fanconi anemia proteins in DNA crosslink repair. Sci Rep. 2016;6:36439 pubmed publisher
  18. Hong X, Liu W, Song R, Shah J, Feng X, Tsang C, et al. SOX9 is targeted for proteasomal degradation by the E3 ligase FBW7 in response to DNA damage. Nucleic Acids Res. 2016;44:8855-8869 pubmed
  19. Schmid Burgk J, Höning K, Ebert T, Hornung V. CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016;7:12338 pubmed publisher
  20. Yard B, Adams D, Chie E, Tamayo P, Battaglia J, Gopal P, et al. A genetic basis for the variation in the vulnerability of cancer to DNA damage. Nat Commun. 2016;7:11428 pubmed publisher
  21. Preet R, Siddharth S, Satapathy S, Das S, Nayak A, Das D, et al. Chk1 inhibitor synergizes quinacrine mediated apoptosis in breast cancer cells by compromising the base excision repair cascade. Biochem Pharmacol. 2016;105:23-33 pubmed publisher
  22. Wang W, Long L, Wang L, Tan C, Fei X, Chen L, et al. Knockdown of Cathepsin L promotes radiosensitivity of glioma stem cells both in vivo and in vitro. Cancer Lett. 2016;371:274-84 pubmed publisher
  23. Harley M, Murina O, Leitch A, Higgs M, Bicknell L, Yigit G, et al. TRAIP promotes DNA damage response during genome replication and is mutated in primordial dwarfism. Nat Genet. 2016;48:36-43 pubmed publisher
  24. Cristini A, Park J, Capranico G, Legube G, Favre G, Sordet O. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res. 2016;44:1161-78 pubmed publisher
  25. Young L, Marzio A, Pérez Durán P, Reid D, Meredith D, Roberti D, et al. TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response. Cell Rep. 2015;13:451-459 pubmed publisher
  26. Renaud E, Barascu A, Rosselli F. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. Nucleic Acids Res. 2016;44:648-56 pubmed publisher
  27. Liu X, Chandramouly G, Rass E, Guan Y, Wang G, Hobbs R, et al. LRF maintains genome integrity by regulating the non-homologous end joining pathway of DNA repair. Nat Commun. 2015;6:8325 pubmed publisher
  28. Damiani E, Puebla Osorio N, Gorbea E, Ullrich S. Platelet-Activating Factor Induces Epigenetic Modifications in Human Mast Cells. J Invest Dermatol. 2015;135:3034-3040 pubmed publisher
  29. Masuda Y, Takahashi H, Sato S, Tomomori Sato C, Saraf A, Washburn M, et al. TRIM29 regulates the assembly of DNA repair proteins into damaged chromatin. Nat Commun. 2015;6:7299 pubmed publisher
  30. Hollingworth R, Skalka G, Stewart G, Hislop A, Blackbourn D, Grand R. Activation of DNA Damage Response Pathways during Lytic Replication of KSHV. Viruses. 2015;7:2908-27 pubmed publisher
  31. Van Sluis M, McStay B. A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage. Genes Dev. 2015;29:1151-63 pubmed publisher
  32. Nakajima N, Hagiwara Y, Oike T, Okayasu R, Murakami T, Nakano T, et al. Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair. PLoS ONE. 2015;10:e0122582 pubmed publisher
  33. Ochi T, Blackford A, Coates J, Jhujh S, Mehmood S, Tamura N, et al. DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science. 2015;347:185-188 pubmed publisher
  34. Alessio N, Del Gaudio S, Capasso S, Di Bernardo G, Cappabianca S, Cipollaro M, et al. Low dose radiation induced senescence of human mesenchymal stromal cells and impaired the autophagy process. Oncotarget. 2015;6:8155-66 pubmed
  35. Sowd G, Mody D, Eggold J, Cortez D, Friedman K, Fanning E. SV40 utilizes ATM kinase activity to prevent non-homologous end joining of broken viral DNA replication products. PLoS Pathog. 2014;10:e1004536 pubmed publisher
  36. Saini R, Attarha S, da Silva Santos C, Kolakowska J, Funa K, Souchelnytskyi S. Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells. Biochem Biophys Res Commun. 2014;454:202-9 pubmed publisher
  37. Owonikoko T, Zhang G, Deng X, Rossi M, Switchenko J, Doho G, et al. Poly (ADP) ribose polymerase enzyme inhibitor, veliparib, potentiates chemotherapy and radiation in vitro and in vivo in small cell lung cancer. Cancer Med. 2014;3:1579-94 pubmed publisher
  38. Britton S, Coates J, Jackson S. A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. J Cell Biol. 2013;202:579-95 pubmed publisher