This is a Validated Antibody Database (VAD) review about human DLL4, based on 11 published articles (read how Labome selects the articles), using DLL4 antibody in all methods. It is aimed to help Labome visitors find the most suited DLL4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DLL4 synonym: AOS6; delta4; hdelta2

R&D Systems
rat monoclonal (207822)
  • immunocytochemistry; human; 1:200; fig 5i
R&D Systems DLL4 antibody (R&D, MAB1389) was used in immunocytochemistry on human samples at 1:200 (fig 5i). elife (2019) ncbi
rat monoclonal (207822)
  • immunocytochemistry; mouse; fig 7b
  • western blot; mouse; fig 7c
R&D Systems DLL4 antibody (R & D, MAB1389) was used in immunocytochemistry on mouse samples (fig 7b) and in western blot on mouse samples (fig 7c). elife (2016) ncbi
rat monoclonal (207822)
  • immunohistochemistry - paraffin section; human; 1 ug/ml
  • immunocytochemistry; human
In order to study the role of Notch signaling in regulating IL-33 expression in quiescent endothelial cells, R&D Systems DLL4 antibody (R&D Systems, 207822) was used in immunohistochemistry - paraffin section on human samples at 1 ug/ml and in immunocytochemistry on human samples . Am J Pathol (2012) ncbi
Invitrogen
mouse monoclonal (MHD4-46)
  • flow cytometry; mouse; 1:1000; loading ...; fig s2
Invitrogen DLL4 antibody (eBioscience, MHD4-1) was used in flow cytometry on mouse samples at 1:1000 (fig s2). J Immunol (2018) ncbi
BioLegend
mouse monoclonal (MHD4-46)
  • blocking or activating experiments; human; 10 ug/ml; loading ...; fig 4a
BioLegend DLL4 antibody (Biolegend, MHD4-46) was used in blocking or activating experiments on human samples at 10 ug/ml (fig 4a). Clin Immunol (2016) ncbi
mouse monoclonal (MHD4-46)
  • flow cytometry; human; fig 3
BioLegend DLL4 antibody (BioLegend, MHD 4-46) was used in flow cytometry on human samples (fig 3). Cytotherapy (2015) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; fig s7b
Abcam DLL4 antibody (Abcam, ab7280) was used in immunohistochemistry - paraffin section on mouse samples (fig s7b). Sci Adv (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3b
  • western blot; mouse; 1:1000; fig 7b
Abcam DLL4 antibody (Abcam, ab7280) was used in western blot on human samples at 1:1000 (fig 3b) and in western blot on mouse samples at 1:1000 (fig 7b). Oncol Lett (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4d
Cell Signaling Technology DLL4 antibody (Cell Signaling, 2589) was used in western blot on human samples (fig 4d). Sci Adv (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3e
Cell Signaling Technology DLL4 antibody (Cell Signaling Technology, 2589) was used in western blot on human samples (fig 3e). Cancer Res (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 7m
In order to characterize parvalbumin interneurons and perineuronal nets in the prefrontal cortex, Cell Signaling Technology DLL4 antibody (Cell signaling, 2589) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 7m). Neuroscience (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 6d
Cell Signaling Technology DLL4 antibody (Cell Signaling, 2589) was used in western blot on human samples (fig 6d). Biochem Pharmacol (2016) ncbi
Articles Reviewed
  1. Liu H, Mei F, Yang W, Wang H, Wong E, Cai J, et al. Epac1 inhibition ameliorates pathological angiogenesis through coordinated activation of Notch and suppression of VEGF signaling. Sci Adv. 2020;6:eaay3566 pubmed publisher
  2. Park M, Kim A, Manandhar S, Oh S, Jang G, Kang L, et al. CCN1 interlinks integrin and hippo pathway to autoregulate tip cell activity. elife. 2019;8: pubmed publisher
  3. Steinbuck M, Arakcheeva K, Winandy S. Novel TCR-Mediated Mechanisms of Notch Activation and Signaling. J Immunol. 2018;200:997-1007 pubmed publisher
  4. Jin L, Vu T, Yuan G, Datta P. STRAP Promotes Stemness of Human Colorectal Cancer via Epigenetic Regulation of the NOTCH Pathway. Cancer Res. 2017;77:5464-5478 pubmed publisher
  5. Ueno H, Suemitsu S, Okamoto M, Matsumoto Y, Ishihara T. Parvalbumin neurons and perineuronal nets in the mouse prefrontal cortex. Neuroscience. 2017;343:115-127 pubmed publisher
  6. Xiao Y, Yang X, Miao Y, He X, Wang M, Sha W. Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways. Oncol Lett. 2016;12:3695-3700 pubmed
  7. Zhang P, He D, Chen Z, Pan Q, Du F, Zang X, et al. Chemotherapy enhances tumor vascularization via Notch signaling-mediated formation of tumor-derived endothelium in breast cancer. Biochem Pharmacol. 2016;118:18-30 pubmed publisher
  8. Ramo K, Sugamura K, Craige S, Keaney J, Davis R. Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development. elife. 2016;5: pubmed publisher
  9. Shen C, Detry B, Lecocq M, Pilette C. A novel IgA/Delta-like 4/Notch axis induces immunosuppressive activity in human dendritic cells. Clin Immunol. 2016;168:37-46 pubmed publisher
  10. Kim H, Huang L, Critser P, Yang Z, Chan R, Wang L, et al. Notch ligand Delta-like 1 promotes in vivo vasculogenesis in human cord blood-derived endothelial colony forming cells. Cytotherapy. 2015;17:579-92 pubmed publisher
  11. Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Küchler A, WEISS M, et al. The alarmin IL-33 is a notch target in quiescent endothelial cells. Am J Pathol. 2012;181:1099-111 pubmed publisher