This is a Validated Antibody Database (VAD) review about human DIAPH1, based on 5 published articles (read how Labome selects the articles), using DIAPH1 antibody in all methods. It is aimed to help Labome visitors find the most suited DIAPH1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DIAPH1 synonym: DFNA1; DIA1; DRF1; LFHL1; SCBMS; hDIA1; protein diaphanous homolog 1

Abcam
rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 5b
Abcam DIAPH1 antibody (Abcam, Ab96784) was used in western blot on human samples at 1:500 (fig 5b). Nat Commun (2018) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 4a
In order to characterize two new families with heterozygous truncating DIAPH1 mutations, Abcam DIAPH1 antibody (Abcam, ab96784) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig 4a). Clin Genet (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:200; fig 3
  • western blot; human; fig 3
Abcam DIAPH1 antibody (Abcam, ab11173) was used in immunocytochemistry on human samples at 1:200 (fig 3) and in western blot on human samples (fig 3). Oncotarget (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human
Abcam DIAPH1 antibody (Abcam, ab11173) was used in immunohistochemistry - paraffin section on human samples . Int J Cancer (2014) ncbi
Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human
In order to develop reagents to study RNA binding proteins, Bethyl DIAPH1 antibody (Bethyl, A300-077A) was used in RNA immunoprecipitation on human samples . Mol Cell (2016) ncbi
Invitrogen
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 4c
In order to characterize two new families with heterozygous truncating DIAPH1 mutations, Invitrogen DIAPH1 antibody (ThermoFisher Scientific, PA5-27607) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 4c). Clin Genet (2017) ncbi
Articles Reviewed
  1. Latham S, Ehmke N, Reinke P, Taft M, Eicke D, Reindl T, et al. Variants in exons 5 and 6 of ACTB cause syndromic thrombocytopenia. Nat Commun. 2018;9:4250 pubmed publisher
  2. Neuhaus C, Lang Roth R, Zimmermann U, Heller R, Eisenberger T, Weikert M, et al. Extension of the clinical and molecular phenotype of DIAPH1-associated autosomal dominant hearing loss (DFNA1). Clin Genet. 2017;91:892-901 pubmed publisher
  3. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  4. Lin Y, Bhuwania R, Gromova K, Failla A, Lange T, Riecken K, et al. Drosophila homologue of Diaphanous 1 (DIAPH1) controls the metastatic potential of colon cancer cells by regulating microtubule-dependent adhesion. Oncotarget. 2015;6:18577-89 pubmed
  5. Lin Y, Izbicki J, König A, Habermann J, Blechner C, Lange T, et al. Expression of DIAPH1 is up-regulated in colorectal cancer and its down-regulation strongly reduces the metastatic capacity of colon carcinoma cells. Int J Cancer. 2014;134:1571-82 pubmed publisher