This is a Validated Antibody Database (VAD) review about human DDX58, based on 33 published articles (read how Labome selects the articles), using DDX58 antibody in all methods. It is aimed to help Labome visitors find the most suited DDX58 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DDX58 synonym: RIG-I; RIG1; RIGI; RLR-1; SGMRT2

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D33H10)
  • western blot knockout validation; human; 1:500; loading ...; fig 4a
Cell Signaling Technology DDX58 antibody (Cell Signaling Technology, 4200) was used in western blot knockout validation on human samples at 1:500 (fig 4a). JCI Insight (2020) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D14G6)
  • western blot knockout validation; mouse; loading ...; fig s8e
Cell Signaling Technology DDX58 antibody (Cell Signaling, 3743) was used in western blot knockout validation on mouse samples (fig s8e). Hepatology (2018) ncbi
Adipogen
monoclonal (Alme-1)
  • western blot knockout validation; human; loading ...; fig 5b
Adipogen DDX58 antibody (Adipogen, Alme-1) was used in western blot knockout validation on human samples (fig 5b). J Virol (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-12)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 3d
  • western blot; human; 1:1000; loading ...; fig 3b
Santa Cruz Biotechnology DDX58 antibody (Santa Cruz, sc-376845) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 3d) and in western blot on human samples at 1:1000 (fig 3b). Cancers (Basel) (2020) ncbi
mouse monoclonal (D-12)
  • western blot; human
Santa Cruz Biotechnology DDX58 antibody (Santa Cruz Biotechnology, 376845) was used in western blot on human samples . Br J Pharmacol (2015) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; 1:5000; loading ...; fig s8
Abcam DDX58 antibody (Abcam, ab45428) was used in western blot on human samples at 1:5000 (fig s8). Nat Microbiol (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 5
Abcam DDX58 antibody (Abcam, ab45428) was used in western blot on mouse samples at 1:1000 (fig 5). Sci Rep (2016) ncbi
Abnova
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3e
Abnova DDX58 antibody (Abnova, PAB15905) was used in western blot on human samples (fig 3e). Nat Microbiol (2017) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3e
MilliporeSigma DDX58 antibody (Sigma, SAB2104315) was used in western blot on human samples (fig 3e). Nat Microbiol (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D14G6)
  • western blot; human; loading ...; fig 6s1a
Cell Signaling Technology DDX58 antibody (Cell Signaling, 3743) was used in western blot on human samples (fig 6s1a). elife (2020) ncbi
domestic rabbit monoclonal (D33H10)
  • western blot knockout validation; human; 1:500; loading ...; fig 4a
Cell Signaling Technology DDX58 antibody (Cell Signaling Technology, 4200) was used in western blot knockout validation on human samples at 1:500 (fig 4a). JCI Insight (2020) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; human; 1:1000; loading ...; fig 3d
Cell Signaling Technology DDX58 antibody (Cell Signaling, D14G6) was used in western blot on human samples at 1:1000 (fig 3d). BMC Biol (2020) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; mouse; loading ...; fig s4f
Cell Signaling Technology DDX58 antibody (Cell Signaling, 3743S) was used in western blot on mouse samples (fig s4f). Science (2019) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; human; loading ...; fig 2h
Cell Signaling Technology DDX58 antibody (Cell Signaling, 3743) was used in western blot on human samples (fig 2h). Cell (2019) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; mouse; loading ...; fig s3a
Cell Signaling Technology DDX58 antibody (Cell Signaling, 3743) was used in western blot on mouse samples (fig s3a). Sci Adv (2019) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; mouse; loading ...
Cell Signaling Technology DDX58 antibody (Cell Signaling Technology, D12G6) was used in western blot on mouse samples . elife (2019) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; mouse; loading ...; fig 7e
Cell Signaling Technology DDX58 antibody (Cell Signaling Technology, D14G6) was used in western blot on mouse samples (fig 7e). Nature (2019) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; human; loading ...; fig s6x
Cell Signaling Technology DDX58 antibody (Cell Signaling Technology, 3743) was used in western blot on human samples (fig s6x). Sci Adv (2018) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; human; loading ...; fig 3f
Cell Signaling Technology DDX58 antibody (Cell Signaling, D14G6) was used in western blot on human samples (fig 3f). PLoS ONE (2018) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot knockout validation; mouse; loading ...; fig s8e
Cell Signaling Technology DDX58 antibody (Cell Signaling, 3743) was used in western blot knockout validation on mouse samples (fig s8e). Hepatology (2018) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; mouse; loading ...; fig 2c
Cell Signaling Technology DDX58 antibody (Cell Signaling, 3743) was used in western blot on mouse samples (fig 2c). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit monoclonal (D14G6)
  • immunoprecipitation; mouse; loading ...; fig 4f
  • western blot; mouse; 1:500; loading ...; fig 3b
  • immunocytochemistry; human; 1:500; loading ...; fig 3f
  • western blot; human; 1:500; loading ...; fig s5f
In order to describe the mechanism by which FBXW7 stabilizes RigI, Cell Signaling Technology DDX58 antibody (Cell Signaling, D14G6) was used in immunoprecipitation on mouse samples (fig 4f), in western blot on mouse samples at 1:500 (fig 3b), in immunocytochemistry on human samples at 1:500 (fig 3f) and in western blot on human samples at 1:500 (fig s5f). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; mouse; loading ...; fig 1c
  • western blot; African green monkey; loading ...; fig 1c
In order to investigate the evolution of RIG-I-like receptors, Cell Signaling Technology DDX58 antibody (Cell signaling, 3743) was used in western blot on mouse samples (fig 1c) and in western blot on African green monkey samples (fig 1c). Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; human; loading ...; fig 7c
Cell Signaling Technology DDX58 antibody (Cell Signaling, 3743) was used in western blot on human samples (fig 7c). J Neuroinflammation (2016) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; mouse; loading ...; fig 3d
Cell Signaling Technology DDX58 antibody (Cell Signaling, D14G6) was used in western blot on mouse samples (fig 3d). Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; human; fig 1
Cell Signaling Technology DDX58 antibody (Cell Signaling, 3743) was used in western blot on human samples (fig 1). Sci Rep (2016) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; human; loading ...; fig 1f
In order to identify the viral RNA signature on RIG-I-like receptors during viral infection, Cell Signaling Technology DDX58 antibody (Cell Signaling, D14G6) was used in western blot on human samples (fig 1f). elife (2016) ncbi
  • western blot; human; fig 1
Cell Signaling Technology DDX58 antibody (Cell signaling, 8348) was used in western blot on human samples (fig 1). Cell Death Dis (2015) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; human
In order to discuss methods for the characterization of stress granules in virus infected cells, Cell Signaling Technology DDX58 antibody (Cell Sig, 3743S) was used in western blot on human samples . Methods (2015) ncbi
domestic rabbit monoclonal (D33H10)
  • western blot; human
In order to discuss methods for the characterization of stress granules in virus infected cells, Cell Signaling Technology DDX58 antibody (Cell Sig, 4200S) was used in western blot on human samples . Methods (2015) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; mouse; fig 1
In order to analyze the antiviral innate immune response due to mitochondrial DNA stress, Cell Signaling Technology DDX58 antibody (Cell Signaling Technology, D14G6) was used in western blot on mouse samples (fig 1). Nature (2015) ncbi
domestic rabbit monoclonal (D14G6)
  • immunoprecipitation; human; fig 3
In order to use three-dimensional stochastic optical reconstruction microscopy to visualize incoming influenza A virus, Cell Signaling Technology DDX58 antibody (Cell Signaling, 3743) was used in immunoprecipitation on human samples (fig 3). Nat Commun (2014) ncbi
domestic rabbit monoclonal (D33H10)
  • western blot; human; fig 6a
Cell Signaling Technology DDX58 antibody (Cell Signaling, 4200T) was used in western blot on human samples (fig 6a). Mol Cancer (2014) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; mouse
In order to study the relationship between adenosine deaminase acting on RNA 1 (ADAR1) and type I IFNs, Cell Signaling Technology DDX58 antibody (Cell Signaling Technology, 3743) was used in western blot on mouse samples . J Immunol (2014) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; human
Cell Signaling Technology DDX58 antibody (Cell Signaling Technology, D14G6) was used in western blot on human samples . PLoS Pathog (2014) ncbi
domestic rabbit monoclonal (D14G6)
  • western blot; human; 1:1,000
In order to study the mechanism for the oncolytic measles virus replication, Cell Signaling Technology DDX58 antibody (Cell Signaling Technologies, 3743) was used in western blot on human samples at 1:1,000. J Virol (2014) ncbi
Adipogen
monoclonal (Alme-1)
  • western blot; human; 1:1000; loading ...; fig s13c
Adipogen DDX58 antibody (AdipoGen, AG-20B-0009-C100) was used in western blot on human samples at 1:1000 (fig s13c). Nat Commun (2019) ncbi
monoclonal (Alme-1)
  • western blot knockout validation; human; loading ...; fig 5b
Adipogen DDX58 antibody (Adipogen, Alme-1) was used in western blot knockout validation on human samples (fig 5b). J Virol (2018) ncbi
Articles Reviewed
  1. Colpitts C, Ridewood S, Schneiderman B, Warne J, Tabata K, Ng C, et al. Hepatitis C virus exploits cyclophilin A to evade PKR. elife. 2020;9: pubmed publisher
  2. de Cubas A, Dunker W, Zaninovich A, Hongo R, Bhatia A, Panda A, et al. DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer. JCI Insight. 2020;5: pubmed publisher
  3. Szymura S, Bernal G, Wu L, Zhang Z, Crawley C, Voce D, et al. DDX39B interacts with the pattern recognition receptor pathway to inhibit NF-κB and sensitize to alkylating chemotherapy. BMC Biol. 2020;18:32 pubmed publisher
  4. Bufalieri F, Caimano M, Lospinoso Severini L, Basili I, Paglia F, Sampirisi L, et al. The RNA-Binding Ubiquitin Ligase MEX3A Affects Glioblastoma Tumorigenesis by Inducing Ubiquitylation and Degradation of RIG-I. Cancers (Basel). 2020;12: pubmed publisher
  5. Lu M, Zhang Z, Xue M, Zhao B, Harder O, Li A, et al. N6-methyladenosine modification enables viral RNA to escape recognition by RNA sensor RIG-I. Nat Microbiol. 2020;5:584-598 pubmed publisher
  6. Moriyama M, Koshiba T, Ichinohe T. Influenza A virus M2 protein triggers mitochondrial DNA-mediated antiviral immune responses. Nat Commun. 2019;10:4624 pubmed publisher
  7. Liu Y, You Y, Lu Z, Yang J, Li P, Liu L, et al. N6-methyladenosine RNA modification-mediated cellular metabolism rewiring inhibits viral replication. Science. 2019;365:1171-1176 pubmed publisher
  8. Zhang W, Wang G, Xu Z, Tu H, Hu F, Dai J, et al. Lactate Is a Natural Suppressor of RLR Signaling by Targeting MAVS. Cell. 2019;: pubmed publisher
  9. Yang S, Harding A, Sweeney C, Miao D, Swan G, Zhou C, et al. Control of antiviral innate immune response by protein geranylgeranylation. Sci Adv. 2019;5:eaav7999 pubmed publisher
  10. Witteveldt J, Knol L, Macias S. MicroRNA-deficient mouse embryonic stem cells acquire a functional interferon response. elife. 2019;8: pubmed publisher
  11. Ishizuka J, Manguso R, Cheruiyot C, Bi K, Panda A, Iracheta Vellve A, et al. Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Nature. 2019;565:43-48 pubmed publisher
  12. Liu Q, Wu Y, Qin Y, Hu J, Xie W, Qin F, et al. Broad and diverse mechanisms used by deubiquitinase family members in regulating the type I interferon signaling pathway during antiviral responses. Sci Adv. 2018;4:eaar2824 pubmed publisher
  13. Ghosh R, Roy S, Franco S. PARP1 depletion induces RIG-I-dependent signaling in human cancer cells. PLoS ONE. 2018;13:e0194611 pubmed publisher
  14. Chiang C, Pauli E, Biryukov J, Feister K, Meng M, White E, et al. The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling. J Virol. 2018;92: pubmed publisher
  15. Wang W, Wang Y, Qu C, Wang S, Zhou J, Cao W, et al. The RNA genome of hepatitis E virus robustly triggers an antiviral interferon response. Hepatology. 2018;67:2096-2112 pubmed publisher
  16. Kazak L, Chouchani E, Stavrovskaya I, Lu G, Jedrychowski M, Egan D, et al. UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction. Proc Natl Acad Sci U S A. 2017;114:7981-7986 pubmed publisher
  17. Song Y, Lai L, Chong Z, He J, Zhang Y, Xue Y, et al. E3 ligase FBXW7 is critical for RIG-I stabilization during antiviral responses. Nat Commun. 2017;8:14654 pubmed publisher
  18. Soonthornvacharin S, Rodriguez Frandsen A, Zhou Y, Galvez F, Huffmaster N, Tripathi S, et al. Systems-based analysis of RIG-I-dependent signalling identifies KHSRP as an inhibitor of RIG-I receptor activation. Nat Microbiol. 2017;2:17022 pubmed publisher
  19. Xu L, Yu D, Fan Y, Peng L, Wu Y, Yao Y. Loss of RIG-I leads to a functional replacement with MDA5 in the Chinese tree shrew. Proc Natl Acad Sci U S A. 2016;113:10950-5 pubmed publisher
  20. Zhang X, Zheng Z, Liu X, Shu B, Mao P, Bai B, et al. Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway. J Neuroinflammation. 2016;13:209 pubmed publisher
  21. Wang W, Jiang M, Liu S, Zhang S, Liu W, Ma Y, et al. RNF122 suppresses antiviral type I interferon production by targeting RIG-I CARDs to mediate RIG-I degradation. Proc Natl Acad Sci U S A. 2016;113:9581-6 pubmed publisher
  22. Hou Z, Zhang J, Han Q, Su C, Qu J, Xu D, et al. Hepatitis B virus inhibits intrinsic RIG-I and RIG-G immune signaling via inducing miR146a. Sci Rep. 2016;6:26150 pubmed publisher
  23. Sanchez David R, Combredet C, Sismeiro O, Dillies M, Jagla B, Coppée J, et al. Comparative analysis of viral RNA signatures on different RIG-I-like receptors. elife. 2016;5:e11275 pubmed publisher
  24. Wu H, Shi L, Wang Q, Cheng L, Zhao X, Chen Q, et al. Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells. Sci Rep. 2016;6:19507 pubmed publisher
  25. Shi J, Fung G, Deng H, Zhang J, Fiesel F, Springer W, et al. NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1. Cell Death Dis. 2015;6:e1943 pubmed publisher
  26. Panas M, Kedersha N, McInerney G. Methods for the characterization of stress granules in virus infected cells. Methods. 2015;90:57-64 pubmed publisher
  27. Ailenberg M, Di Ciano Oliveira C, Szaszi K, Dan Q, Rozycki M, Kapus A, et al. Dynasore enhances the formation of mitochondrial antiviral signalling aggregates and endocytosis-independent NF-κB activation. Br J Pharmacol. 2015;172:3748-63 pubmed publisher
  28. West A, Khoury Hanold W, Staron M, Tal M, Pineda C, Lang S, et al. Mitochondrial DNA stress primes the antiviral innate immune response. Nature. 2015;520:553-7 pubmed publisher
  29. Liedmann S, Hrincius E, Guy C, Anhlan D, Dierkes R, Carter R, et al. Viral suppressors of the RIG-I-mediated interferon response are pre-packaged in influenza virions. Nat Commun. 2014;5:5645 pubmed publisher
  30. Harashima N, Minami T, Uemura H, Harada M. Transfection of poly(I:C) can induce reactive oxygen species-triggered apoptosis and interferon-β-mediated growth arrest in human renal cell carcinoma cells via innate adjuvant receptors and the 2-5A system. Mol Cancer. 2014;13:217 pubmed publisher
  31. Yang S, Deng P, Zhu Z, Zhu J, Wang G, Zhang L, et al. Adenosine deaminase acting on RNA 1 limits RIG-I RNA detection and suppresses IFN production responding to viral and endogenous RNAs. J Immunol. 2014;193:3436-45 pubmed publisher
  32. Chattergoon M, Latanich R, Quinn J, Winter M, Buckheit R, Blankson J, et al. HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon. PLoS Pathog. 2014;10:e1004082 pubmed publisher
  33. Xia M, Gonzalez P, Li C, Meng G, Jiang A, Wang H, et al. Mitophagy enhances oncolytic measles virus replication by mitigating DDX58/RIG-I-like receptor signaling. J Virol. 2014;88:5152-64 pubmed publisher