This is a Validated Antibody Database (VAD) review about human DDX3, based on 11 published articles (read how Labome selects the articles), using DDX3 antibody in all methods. It is aimed to help Labome visitors find the most suited DDX3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DDX3 synonym: CAP-Rf; DBX; DDX14; DDX3; HLP2; MRX102; ATP-dependent RNA helicase DDX3X; DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; DEAD/H box-3; helicase-like protein 2

Abcam
rabbit polyclonal
  • western blot; human; loading ...; fig 4e
Abcam DDX3 antibody (Abcam, ab37160) was used in western blot on human samples (fig 4e). Cancer Res (2019) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5b
Abcam DDX3 antibody (Abcam, ab37160) was used in western blot on mouse samples (fig 5b). J Clin Invest (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig 6
  • immunohistochemistry - paraffin section; human; fig 6
In order to study the composition of the protein aggregate hallmark of Alexander disease called rosenthal fibers, Abcam DDX3 antibody (Abcam, ab61153) was used in immunohistochemistry on mouse samples at 1:100 (fig 6) and in immunohistochemistry - paraffin section on human samples (fig 6). J Proteome Res (2016) ncbi
rabbit polyclonal
  • western blot; human
Abcam DDX3 antibody (Abcam, ab37160) was used in western blot on human samples . PLoS ONE (2012) ncbi
LifeSpan Biosciences
mouse monoclonal
  • immunocytochemistry; human
LifeSpan Biosciences DDX3 antibody (LifeSpan, LS-C64576) was used in immunocytochemistry on human samples . Biochem Biophys Res Commun (2013) ncbi
mouse monoclonal
  • immunocytochemistry; human
LifeSpan Biosciences DDX3 antibody (LifeSpan BioSciences, LS-C64576) was used in immunocytochemistry on human samples . Biochem Biophys Res Commun (2013) ncbi
mouse monoclonal
  • immunocytochemistry; human; fig 6
LifeSpan Biosciences DDX3 antibody (Life Span, LS-C64576) was used in immunocytochemistry on human samples (fig 6). J Virol (2011) ncbi
Santa Cruz Biotechnology
mouse monoclonal (2253C5a)
  • western blot; human; 1:500; fig 5
  • western blot; mouse; 1:500; fig 5
Santa Cruz Biotechnology DDX3 antibody (Santa Cruz Biotechnology, sc-81247) was used in western blot on human samples at 1:500 (fig 5) and in western blot on mouse samples at 1:500 (fig 5). Mol Cell Biol (2015) ncbi
GeneTex
rabbit polyclonal
  • RNA immunoprecipitation; human
In order to develop reagents to study RNA binding proteins, GeneTex DDX3 antibody (GeneTex, GTX110614) was used in RNA immunoprecipitation on human samples . Mol Cell (2016) ncbi
MilliporeSigma
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 3a
In order to elucidate how Fused in Sarcoma/Translocated in Liposarcoma contributes to disease, MilliporeSigma DDX3 antibody (Sigma, HPA001648) was used in immunocytochemistry on human samples (fig 4b) and in western blot on human samples (fig 3a). Biochim Biophys Acta (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (D19B4)
  • western blot; human; 1:2000; fig 3
  • western blot; mouse; 1:2000; fig 2
In order to study the composition of the protein aggregate hallmark of Alexander disease called rosenthal fibers, Cell Signaling Technology DDX3 antibody (Cell Signaling, D19B4) was used in western blot on human samples at 1:2000 (fig 3) and in western blot on mouse samples at 1:2000 (fig 2). J Proteome Res (2016) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 2a
EMD Millipore DDX3 antibody (Millipore, 09-860) was used in western blot on human samples at 1:1000 (fig 2a). J Immunol (2016) ncbi
Articles Reviewed
  1. Yang F, Fang E, Mei H, Chen Y, Li H, Li D, et al. Cis-Acting circ-CTNNB1 Promotes β-Catenin Signaling and Cancer Progression via DDX3-Mediated Transactivation of YY1. Cancer Res. 2019;79:557-571 pubmed publisher
  2. Wang J, Rajbhandari P, Damianov A, Han A, Sallam T, Waki H, et al. RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs. J Clin Invest. 2017;127:987-1004 pubmed publisher
  3. Kamelgarn M, Chen J, Kuang L, Arenas A, Zhai J, Zhu H, et al. Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS. Biochim Biophys Acta. 2016;1862:2004-14 pubmed publisher
  4. Reches A, Nachmani D, Berhani O, Duev Cohen A, Shreibman D, Ophir Y, et al. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins. J Immunol. 2016;196:4967-76 pubmed publisher
  5. Heaven M, Flint D, Randall S, Sosunov A, Wilson L, Barnes S, et al. Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease. J Proteome Res. 2016;15:2265-82 pubmed publisher
  6. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  7. Çelik H, Sajwan K, Selvanathan S, Marsh B, Pai A, Kont Y, et al. Ezrin Binds to DEAD-Box RNA Helicase DDX3 and Regulates Its Function and Protein Level. Mol Cell Biol. 2015;35:3145-62 pubmed publisher
  8. Yasuda Inoue M, Kuroki M, Ariumi Y. DDX3 RNA helicase is required for HIV-1 Tat function. Biochem Biophys Res Commun. 2013;441:607-11 pubmed publisher
  9. Yasuda Inoue M, Kuroki M, Ariumi Y. Distinct DDX DEAD-box RNA helicases cooperate to modulate the HIV-1 Rev function. Biochem Biophys Res Commun. 2013;434:803-8 pubmed publisher
  10. Jarboui M, Bidoia C, Woods E, Roe B, Wynne K, Elia G, et al. Nucleolar protein trafficking in response to HIV-1 Tat: rewiring the nucleolus. PLoS ONE. 2012;7:e48702 pubmed publisher
  11. Ariumi Y, Kuroki M, Kushima Y, Osugi K, Hijikata M, Maki M, et al. Hepatitis C virus hijacks P-body and stress granule components around lipid droplets. J Virol. 2011;85:6882-92 pubmed publisher