This is a Validated Antibody Database (VAD) review about human DDB1, based on 21 published articles (read how Labome selects the articles), using DDB1 antibody in all methods. It is aimed to help Labome visitors find the most suited DDB1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DDB1 synonym: DDBA; UV-DDB1; XAP1; XPCE; XPE; XPE-BF; DNA damage-binding protein 1; DDB p127 subunit; DNA damage-binding protein a; HBV X-associated protein 1; UV-DDB 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; XAP-1; XPE-binding factor; damage-specific DNA binding protein 1, 127kDa; xeroderma pigmentosum group E-complementing protein

Knockout validation
Bethyl
rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:400; fig s1
Bethyl DDB1 antibody (Bethyl, IHC-0013401) was used in immunohistochemistry knockout validation on mouse samples at 1:400 (fig s1). Nat Commun (2015) ncbi
Abcam
rabbit monoclonal (EPR6089)
  • immunoprecipitation; human; loading ...; fig 4a
Abcam DDB1 antibody (AbCam, ab109027) was used in immunoprecipitation on human samples (fig 4a). elife (2019) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:100; loading ...; fig s8c
Abcam DDB1 antibody (Abcam, ab97522) was used in immunohistochemistry on human samples at 1:100 (fig s8c). Nat Commun (2018) ncbi
rabbit monoclonal (EPR6089)
  • western blot; human; 1:10,000; loading ...; fig 3e
Abcam DDB1 antibody (Abcam, ab109027) was used in western blot on human samples at 1:10,000 (fig 3e). Science (2017) ncbi
Invitrogen
mouse monoclonal (2B12D1)
  • western blot; human; loading ...; fig 5a
In order to show that the Abl-DDB1-DDA1 axis reduces CRL4 substrate ubiquitination, Invitrogen DDB1 antibody (Invitrogen, 37-6200) was used in western blot on human samples (fig 5a). J Biol Chem (2017) ncbi
mouse monoclonal (2B12D1)
  • western blot; human; fig 4
In order to analyze the divergent interactions with UNG2 and HLTF DNA repair proteins by HIV-1 and HIV-2, Invitrogen DDB1 antibody (Invitrogen, 37-6200) was used in western blot on human samples (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (ZD001)
  • western blot; human; 1:1000; fig 3
In order to study UVA response and nucleotide excision repair deficiency in melanoma, Invitrogen DDB1 antibody (Invitrogen, ZD001) was used in western blot on human samples at 1:1000 (fig 3). Exp Hematol Oncol (2015) ncbi
mouse monoclonal (2B12D1)
  • western blot; human; fig 2
In order to investigate how ubiquitination of BK channels contributes to epilepsy, Invitrogen DDB1 antibody (Invitrogen, 37-6200) was used in western blot on human samples (fig 2). Nat Commun (2014) ncbi
mouse monoclonal (2B12D1)
  • western blot; human; fig 1a
In order to suggest that lentiviral Vpx usurps VprBP-associated Cullin 4 ubiquitin ligase to allow for efficient reverse transcription, Invitrogen DDB1 antibody (Zymed/Invitrogen, 37-6200) was used in western blot on human samples (fig 1a). PLoS Pathog (2008) ncbi
Bethyl
rabbit polyclonal
  • immunohistochemistry; mouse; 1:500; loading ...; fig 7f
Bethyl DDB1 antibody (Bethyl, IHC-00134) was used in immunohistochemistry on mouse samples at 1:500 (fig 7f). Cell Rep (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 3a
In order to identify homologous to the E6-AP carboxyl terminus as a ubiquitin ligase for modulator of apoptosis protein 1, Bethyl DDB1 antibody (Bethyl Laboratories, A300-462A) was used in western blot on human samples (fig 3a). Oncogene (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 1
In order to study disruption of the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity due to immunomodulatory drugs, Bethyl DDB1 antibody (Bethyl Laboratories, A300-462A) was used in western blot on human samples at 1:1000 (fig 1). Nat Med (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 3f
Bethyl DDB1 antibody (Bethyl Laboratories, A300-462A) was used in western blot on human samples (fig 3f). Biochem Biophys Res Commun (2015) ncbi
rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:400; fig s1
Bethyl DDB1 antibody (Bethyl, IHC-0013401) was used in immunohistochemistry knockout validation on mouse samples at 1:400 (fig s1). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 3
Bethyl DDB1 antibody (Bethyl Laboratories, A300-462A) was used in western blot on human samples at 1:1000 (fig 3). Cell Cycle (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-1)
  • western blot; mouse; loading ...; fig 3d
In order to use knockout mice to determine the role of cereblon in T cells, Santa Cruz Biotechnology DDB1 antibody (Santa Cruz, B-1) was used in western blot on mouse samples (fig 3d). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (8)
  • western blot; human; 1:500; fig 4
Santa Cruz Biotechnology DDB1 antibody (Santa Cruz, sc-136180) was used in western blot on human samples at 1:500 (fig 4). FASEB J (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (D4C8)
  • western blot; human; 1:1000; loading ...; fig s8b
Cell Signaling Technology DDB1 antibody (Cell Signaling, 6998) was used in western blot on human samples at 1:1000 (fig s8b). Nat Commun (2018) ncbi
rabbit monoclonal (D4C8)
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology DDB1 antibody (CST, 6998) was used in western blot on human samples (fig 2c). Genes Dev (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4e
Cell Signaling Technology DDB1 antibody (Cell Signaling, S428) was used in western blot on human samples at 1:1000 (fig 4e). Oncotarget (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 2
Cell Signaling Technology DDB1 antibody (Cell Signaling Technology, 5428) was used in western blot on human samples at 1:1000 (fig 2). Nature (2016) ncbi
rabbit monoclonal (D4C8)
  • western blot; human; fig 5c
Cell Signaling Technology DDB1 antibody (Cell Signaling, 6998) was used in western blot on human samples (fig 5c). PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; human
In order to study how phosphorylation alters COP1-binding sites of oncoproteins, Cell Signaling Technology DDB1 antibody (Cell Signaling, 5428S) was used in western blot on human samples . Cancer Cell (2014) ncbi
Articles Reviewed
  1. Silva M, Ferguson F, Cai Q, Donovan K, Nandi G, Patnaik D, et al. Targeted degradation of aberrant tau in frontotemporal dementia patient-derived neuronal cell models. elife. 2019;8: pubmed publisher
  2. Zhu B, Chen S, Wang H, Yin C, Han C, Peng C, et al. The protective role of DOT1L in UV-induced melanomagenesis. Nat Commun. 2018;9:259 pubmed publisher
  3. Morgan M, Rickels R, Collings C, He X, Cao K, Herz H, et al. A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation. Genes Dev. 2017;31:2003-2014 pubmed publisher
  4. Han T, Goralski M, Gaskill N, Capota E, Kim J, Ting T, et al. Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science. 2017;356: pubmed publisher
  5. Li G, Ji T, Chen J, Fu Y, Hou L, Feng Y, et al. CRL4DCAF8 Ubiquitin Ligase Targets Histone H3K79 and Promotes H3K9 Methylation in the Liver. Cell Rep. 2017;18:1499-1511 pubmed publisher
  6. Gao S, Geng C, Song T, Lin X, Liu J, Cai Z, et al. Activation of c-Abl Kinase Potentiates the Anti-myeloma Drug Lenalidomide by Promoting DDA1 Protein Recruitment to the CRL4 Ubiquitin Ligase. J Biol Chem. 2017;292:3683-3691 pubmed publisher
  7. Matsuura K, Huang N, Cocce K, Zhang L, Kornbluth S. Downregulation of the proapoptotic protein MOAP-1 by the UBR5 ubiquitin ligase and its role in ovarian cancer resistance to cisplatin. Oncogene. 2017;36:1698-1706 pubmed publisher
  8. Prince E, Balakrishnan I, Shah M, Mulcahy Levy J, Griesinger A, Alimova I, et al. Checkpoint kinase 1 expression is an adverse prognostic marker and therapeutic target in MYC-driven medulloblastoma. Oncotarget. 2016;7:53881-53894 pubmed publisher
  9. Kang J, Park S, Jeong S, Han M, Lee C, Lee K, et al. Epigenetic regulation of Kcna3-encoding Kv1.3 potassium channel by cereblon contributes to regulation of CD4+ T-cell activation. Proc Natl Acad Sci U S A. 2016;113:8771-6 pubmed publisher
  10. Hrecka K, Hao C, Shun M, Kaur S, Swanson S, Florens L, et al. HIV-1 and HIV-2 exhibit divergent interactions with HLTF and UNG2 DNA repair proteins. Proc Natl Acad Sci U S A. 2016;113:E3921-30 pubmed publisher
  11. Eichner R, Heider M, Fernández Sáiz V, van Bebber F, Garz A, Lemeer S, et al. Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity. Nat Med. 2016;22:735-43 pubmed publisher
  12. Decorsière A, Mueller H, van Breugel P, Abdul F, Gerossier L, Beran R, et al. Hepatitis B virus X protein identifies the Smc5/6 complex as a host restriction factor. Nature. 2016;531:386-9 pubmed publisher
  13. Murray H, Maltby V, Smith D, Bowden N. Nucleotide excision repair deficiency in melanoma in response to UVA. Exp Hematol Oncol. 2015;5:6 pubmed publisher
  14. Lyu L, Whitcomb E, Jiang S, Chang M, Gu Y, Duncan M, et al. Unfolded-protein response-associated stabilization of p27(Cdkn1b) interferes with lens fiber cell denucleation, leading to cataract. FASEB J. 2016;30:1087-95 pubmed publisher
  15. Xie X, Wang M, Mei J, Hu F, Ding F, Lv L. Pyruvate kinase M2 interacts with DNA damage-binding protein 2 and reduces cell survival upon UV irradiation. Biochem Biophys Res Commun. 2015;467:427-33 pubmed publisher
  16. Yu C, Ji S, Sha Q, Sun Q, Fan H. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun. 2015;6:8017 pubmed publisher
  17. Sjögren B, Swaney S, Neubig R. FBXO44-Mediated Degradation of RGS2 Protein Uniquely Depends on a Cullin 4B/DDB1 Complex. PLoS ONE. 2015;10:e0123581 pubmed publisher
  18. Han C, Wani G, Zhao R, Qian J, Sharma N, He J, et al. Cdt2-mediated XPG degradation promotes gap-filling DNA synthesis in nucleotide excision repair. Cell Cycle. 2015;14:1103-15 pubmed publisher
  19. Lu G, Zhang Q, Huang Y, Song J, Tomaino R, Ehrenberger T, et al. Phosphorylation of ETS1 by Src family kinases prevents its recognition by the COP1 tumor suppressor. Cancer Cell. 2014;26:222-34 pubmed publisher
  20. Liu J, Ye J, Zou X, Xu Z, Feng Y, Zou X, et al. CRL4A(CRBN) E3 ubiquitin ligase restricts BK channel activity and prevents epileptogenesis. Nat Commun. 2014;5:3924 pubmed publisher
  21. Srivastava S, Swanson S, Manel N, Florens L, Washburn M, Skowronski J. Lentiviral Vpx accessory factor targets VprBP/DCAF1 substrate adaptor for cullin 4 E3 ubiquitin ligase to enable macrophage infection. PLoS Pathog. 2008;4:e1000059 pubmed publisher