This is a Validated Antibody Database (VAD) review about human DC SIGN, based on 31 published articles (read how Labome selects the articles), using DC SIGN antibody in all methods. It is aimed to help Labome visitors find the most suited DC SIGN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DC SIGN synonym: CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1; CD209 antigen; C-type lectin domain family 4 member L; HIV gpl20-binding protein; dendritic cell-specific ICAM-3-grabbing non-integrin 1; dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin; dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin

Knockout validation
BioLegend
mouse monoclonal (9E9A8)
  • immunohistochemistry knockout validation; mouse; fig s6b
  • immunohistochemistry - frozen section; mouse; fig s6b
BioLegend DC SIGN antibody (BioLegend, 330112) was used in immunohistochemistry knockout validation on mouse samples (fig s6b) and in immunohistochemistry - frozen section on mouse samples (fig s6b). Cell (2018) ncbi
Abcam
mouse monoclonal (120507)
  • western blot knockout validation; human; loading ...; fig 2d
  • blocking or activating experiments; human; 10 ug/ml; loading ...
In order to demonstrate a novel mechanism by which HIV-1 invades ocular tissues and promotes translocation or invasion by additional pathogens, Abcam DC SIGN antibody (Abcam, 120507) was used in western blot knockout validation on human samples (fig 2d) and in blocking or activating experiments on human samples at 10 ug/ml. J Biol Chem (2016) ncbi
Invitrogen
rat monoclonal (eB-h209)
  • flow cytometry; human; loading ...; fig 1c
In order to elucidate the role of pattern recognition receptors in Bechet's disease, Invitrogen DC SIGN antibody (eBioscience, 12-2099-42) was used in flow cytometry on human samples (fig 1c). Mediators Inflamm (2017) ncbi
rat monoclonal (eB-h209)
  • flow cytometry; human; loading ...; fig 1a
In order to demonstrate a novel mechanism by which HIV-1 invades ocular tissues and promotes translocation or invasion by additional pathogens, Invitrogen DC SIGN antibody (eBioscience, eB-h209) was used in flow cytometry on human samples (fig 1a). J Biol Chem (2016) ncbi
rat monoclonal (eB-h209)
  • flow cytometry; human; loading ...; fig 2d
Invitrogen DC SIGN antibody (eBioscience, eB-h209) was used in flow cytometry on human samples (fig 2d). Sci Rep (2016) ncbi
rat monoclonal (eB-h209)
  • flow cytometry; human; 1:100; fig 2
Invitrogen DC SIGN antibody (ebioscience, eB-h209) was used in flow cytometry on human samples at 1:100 (fig 2). PLoS ONE (2016) ncbi
rat monoclonal (eB-h209)
  • flow cytometry; human
Invitrogen DC SIGN antibody (eBioscience, eB-h209) was used in flow cytometry on human samples . J Immunol (2014) ncbi
rat monoclonal (eB-h209)
  • flow cytometry; human; fig 8
In order to explore the role of HIV in potentially modulating macrophage function, Invitrogen DC SIGN antibody (eBioscience, eB-h209) was used in flow cytometry on human samples (fig 8). Retrovirology (2013) ncbi
rat monoclonal (eB-h209)
  • flow cytometry; human; tbl 2
In order to investigate the impact of HSP-27 treatment on monocyte differentiation into dendritic cells, Invitrogen DC SIGN antibody (eBioscience, eB-h209) was used in flow cytometry on human samples (tbl 2). Eur J Immunol (2007) ncbi
BioLegend
mouse monoclonal (9E9A8)
  • immunohistochemistry knockout validation; mouse; fig s6b
  • immunohistochemistry - frozen section; mouse; fig s6b
BioLegend DC SIGN antibody (BioLegend, 330112) was used in immunohistochemistry knockout validation on mouse samples (fig s6b) and in immunohistochemistry - frozen section on mouse samples (fig s6b). Cell (2018) ncbi
mouse monoclonal (9E9A8)
  • flow cytometry; human; loading ...; fig 2a
In order to study the involvement of RANKL in decidual M2 macrophage polarization, BioLegend DC SIGN antibody (Biolegend, 330110) was used in flow cytometry on human samples (fig 2a). Cell Death Dis (2017) ncbi
mouse monoclonal (9E9A8)
  • flow cytometry; human; loading ...; fig 6b
BioLegend DC SIGN antibody (Biolegend, 9E9A8) was used in flow cytometry on human samples (fig 6b). J Immunol (2014) ncbi
R&D Systems
mouse monoclonal (120507)
  • blocking or activating experiments; rat; loading ...; fig 2h
R&D Systems DC SIGN antibody (R&D Systems, 120507) was used in blocking or activating experiments on rat samples (fig 2h). Sci Rep (2016) ncbi
mouse monoclonal (DC28)
  • ELISA; human; 0.5 ug/ml
R&D Systems DC SIGN antibody (R&D Systems, MAB16211) was used in ELISA on human samples at 0.5 ug/ml. Mol Cell Biochem (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (MR-1)
  • flow cytometry; rat; loading ...; fig s3g
Santa Cruz Biotechnology DC SIGN antibody (SantaCruz, MR-1) was used in flow cytometry on rat samples (fig s3g). Sci Rep (2016) ncbi
mouse monoclonal (B-2)
  • western blot; human
Santa Cruz Biotechnology DC SIGN antibody (Santa Cruz, sc-74589) was used in western blot on human samples . J Biomed Mater Res A (2015) ncbi
Abcam
mouse monoclonal (120507)
  • western blot knockout validation; human; loading ...; fig 2d
  • blocking or activating experiments; human; 10 ug/ml; loading ...
In order to demonstrate a novel mechanism by which HIV-1 invades ocular tissues and promotes translocation or invasion by additional pathogens, Abcam DC SIGN antibody (Abcam, 120507) was used in western blot knockout validation on human samples (fig 2d) and in blocking or activating experiments on human samples at 10 ug/ml. J Biol Chem (2016) ncbi
mouse monoclonal (120507)
  • blocking or activating experiments; human
Abcam DC SIGN antibody (Abcam, ab13487) was used in blocking or activating experiments on human samples . Apoptosis (2013) ncbi
Bio-Rad
rabbit polyclonal
  • immunohistochemistry - frozen section; sheep
In order to characterize the cells transporting prion protein aggregates across the intestinal mucosa of sheep, Bio-Rad DC SIGN antibody (Serotec, AHP627) was used in immunohistochemistry - frozen section on sheep samples . Prion (2012) ncbi
Beckman Coulter
mouse monoclonal (AZND1)
  • blocking or activating experiments; human; 2 ug/ml
Beckman Coulter DC SIGN antibody (Beckman Coulter, A07406) was used in blocking or activating experiments on human samples at 2 ug/ml. PLoS ONE (2015) ncbi
BD Biosciences
mouse monoclonal (DCN46)
  • flow cytometry; human; 1:100; loading ...; fig 4a
BD Biosciences DC SIGN antibody (BD Biosciences, 551264) was used in flow cytometry on human samples at 1:100 (fig 4a). Nat Commun (2018) ncbi
mouse monoclonal (DCN46)
  • immunohistochemistry; human; loading ...; fig 3a
BD Biosciences DC SIGN antibody (BD Biosciences, DCN46) was used in immunohistochemistry on human samples (fig 3a). J Exp Med (2018) ncbi
mouse monoclonal (DCN46)
  • flow cytometry; human; tbl 1
In order to compare methods of isolating skin mononuclear phagocytes, BD Biosciences DC SIGN antibody (Becton Dickinson, DCN46) was used in flow cytometry on human samples (tbl 1). J Leukoc Biol (2017) ncbi
mouse monoclonal (DCN46)
  • western blot; human; loading ...; fig 4c
In order to research the HIV-1 infection in dendritic cell subsets, BD Biosciences DC SIGN antibody (BD Biosciences, 551186) was used in western blot on human samples (fig 4c). Nature (2016) ncbi
mouse monoclonal (DCN46)
  • flow cytometry; human; loading ...
In order to compare the effect of CXCL4 and its variant CXCL4L1 on the differentiation of monocytes into macrophages and into immature monocyte-derived dendritic cells, BD Biosciences DC SIGN antibody (BD Biosciences, DCN46) was used in flow cytometry on human samples . PLoS ONE (2016) ncbi
mouse monoclonal (DCN46)
  • flow cytometry; human
In order to evaluate combinations of TLR and C-type lectin receptor agonists on the Th1 responses of newborn dendritic cells, BD Biosciences DC SIGN antibody (BD Biosciences, DCN46) was used in flow cytometry on human samples . J Immunol (2016) ncbi
mouse monoclonal (DCN46)
  • flow cytometry; human; fig 2E
BD Biosciences DC SIGN antibody (BD, 558263) was used in flow cytometry on human samples (fig 2E). Reproduction (2016) ncbi
mouse monoclonal (DCN46)
  • flow cytometry; human; fig 5c
BD Biosciences DC SIGN antibody (BD Biosciences, DCN46) was used in flow cytometry on human samples (fig 5c). J Immunol (2016) ncbi
mouse monoclonal (DCN46)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences DC SIGN antibody (BD, 551265) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (DCN46)
  • flow cytometry; human; fig 3
In order to study human cord blood and bone marrow for restricted dendritic cell and monocyte progenitors, BD Biosciences DC SIGN antibody (BD, DCN46) was used in flow cytometry on human samples (fig 3). J Exp Med (2015) ncbi
mouse monoclonal (DCN46)
  • immunocytochemistry; human
BD Biosciences DC SIGN antibody (BD Pharmingen, 551186) was used in immunocytochemistry on human samples . Front Phys (2014) ncbi
mouse monoclonal (DCN46)
  • flow cytometry; human
In order to discuss how inflammation contributes to pulmonary arterial hypertension, BD Biosciences DC SIGN antibody (BD Pharmingen, clone DCN46) was used in flow cytometry on human samples . Chest (2015) ncbi
mouse monoclonal (DCN46)
  • flow cytometry; human
BD Biosciences DC SIGN antibody (BD Biosciences, DCN46) was used in flow cytometry on human samples . J Immunol (2014) ncbi
mouse monoclonal (DCN46)
  • immunohistochemistry; human
BD Biosciences DC SIGN antibody (BD Pharmingen, clone DCN46) was used in immunohistochemistry on human samples . AIDS Res Hum Retroviruses (2014) ncbi
mouse monoclonal (DCN46)
  • flow cytometry; human
In order to describe protocols for immunostaining and multiparameter flow cytometric analysis of major human antigen-presenting cells in peripheral blood, BD Biosciences DC SIGN antibody (BD, 558263) was used in flow cytometry on human samples . Nat Protoc (2010) ncbi
Articles Reviewed
  1. Murakami T, Kim J, Li Y, Green G, Shikanov A, Ono A. Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions. Nat Commun. 2018;9:2436 pubmed publisher
  2. Pagan J, Kitaoka M, Anthony R. Engineered Sialylation of Pathogenic Antibodies In Vivo Attenuates Autoimmune Disease. Cell. 2018;172:564-577.e13 pubmed publisher
  3. Bujko A, Atlasy N, Landsverk O, Richter L, Yaqub S, Horneland R, et al. Transcriptional and functional profiling defines human small intestinal macrophage subsets. J Exp Med. 2018;215:441-458 pubmed publisher
  4. Meng Y, Zhou W, Jin L, Liu L, Chang K, Mei J, et al. RANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization. Cell Death Dis. 2017;8:e3105 pubmed publisher
  5. Choi B, Suh C, Kim H, Sayeed H, Sohn S. The Correlation of CD206, CD209, and Disease Severity in Behçet's Disease with Arthritis. Mediators Inflamm. 2017;2017:7539529 pubmed publisher
  6. Botting R, Bertram K, Baharlou H, Sandgren K, Fletcher J, Rhodes J, et al. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes. J Leukoc Biol. 2017;101:1393-1403 pubmed publisher
  7. Ribeiro C, Sarrami Forooshani R, Setiawan L, Zijlstra Willems E, van Hamme J, Tigchelaar W, et al. Receptor usage dictates HIV-1 restriction by human TRIM5? in dendritic cell subsets. Nature. 2016;540:448-452 pubmed publisher
  8. Gouwy M, Ruytinx P, Radice E, Claudi F, Van Raemdonck K, Bonecchi R, et al. CXCL4 and CXCL4L1 Differentially Affect Monocyte Survival and Dendritic Cell Differentiation and Phagocytosis. PLoS ONE. 2016;11:e0166006 pubmed publisher
  9. Xin G, Wei Z, Ji C, Zheng H, Gu J, Ma L, et al. Metformin Uniquely Prevents Thrombosis by Inhibiting Platelet Activation and mtDNA Release. Sci Rep. 2016;6:36222 pubmed publisher
  10. van Haren S, Dowling D, Foppen W, Christensen D, Andersen P, Reed S, et al. Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization. J Immunol. 2016;197:4413-4424 pubmed
  11. Qian Y, Li C, Jiang A, Ge S, Gu P, Fan X, et al. HIV-1 gp120 Glycoprotein Interacting with Dendritic Cell-specific Intercellular Adhesion Molecule 3-grabbing Non-integrin (DC-SIGN) Down-Regulates Tight Junction Proteins to Disrupt the Blood Retinal Barrier and Increase Its Permeability. J Biol Chem. 2016;291:22977-22987 pubmed
  12. Loegl J, Hiden U, Nussbaumer E, Schliefsteiner C, Cvitic S, Lang I, et al. Hofbauer cells of M2a, M2b and M2c polarization may regulate feto-placental angiogenesis. Reproduction. 2016;152:447-55 pubmed publisher
  13. Paquin Proulx D, Gibbs A, Bachle S, Checa A, Introini A, Leeansyah E, et al. Innate Invariant NKT Cell Recognition of HIV-1-Infected Dendritic Cells Is an Early Detection Mechanism Targeted by Viral Immune Evasion. J Immunol. 2016;197:1843-51 pubmed publisher
  14. Harfuddin Z, Dharmadhikari B, Wong S, Duan K, Poidinger M, Kwajah S, et al. Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype. Sci Rep. 2016;6:29712 pubmed publisher
  15. Leone D, Kozakowski N, Kornauth C, Waidacher T, Neudert B, Loeffler A, et al. The Phenotypic Characterization of the Human Renal Mononuclear Phagocytes Reveal a Co-Ordinated Response to Injury. PLoS ONE. 2016;11:e0151674 pubmed publisher
  16. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  17. Liu X, Zhang H, SU L, Yang P, Xin Z, Zou J, et al. Low expression of dendritic cell-specific intercellular adhesion molecule-grabbing nonintegrin-related protein in lung cancer and significant correlations with brain metastasis and natural killer cells. Mol Cell Biochem. 2015;407:151-60 pubmed publisher
  18. Konieczna P, Schiavi E, Ziegler M, Groeger D, Healy S, Grant R, et al. Human dendritic cell DC-SIGN and TLR-2 mediate complementary immune regulatory activities in response to Lactobacillus rhamnosus JB-1. PLoS ONE. 2015;10:e0120261 pubmed publisher
  19. Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
  20. Itano M, Graus M, Pehlke C, Wester M, Liu P, Lidke K, et al. Super-resolution imaging of C-type lectin spatial rearrangement within the dendritic cell plasma membrane at fungal microbe contact sites. Front Phys. 2014;2: pubmed
  21. Hautefort A, Girerd B, Montani D, Cohen Kaminsky S, Price L, Lambrecht B, et al. T-helper 17 cell polarization in pulmonary arterial hypertension. Chest. 2015;147:1610-1620 pubmed publisher
  22. Davey M, Morgan M, Liuzzi A, Tyler C, Khan M, Szakmany T, et al. Microbe-specific unconventional T cells induce human neutrophil differentiation into antigen cross-presenting cells. J Immunol. 2014;193:3704-3716 pubmed publisher
  23. McNally A, Anderson J. Phenotypic expression in human monocyte-derived interleukin-4-induced foreign body giant cells and macrophages in vitro: dependence on material surface properties. J Biomed Mater Res A. 2015;103:1380-90 pubmed publisher
  24. Balan S, Ollion V, Colletti N, Chelbi R, Montanana Sanchis F, Liu H, et al. Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells. J Immunol. 2014;193:1622-35 pubmed publisher
  25. Fredriksson K, Mishra A, Lam J, Mushaben E, Cuento R, Meyer K, et al. The very low density lipoprotein receptor attenuates house dust mite-induced airway inflammation by suppressing dendritic cell-mediated adaptive immune responses. J Immunol. 2014;192:4497-509 pubmed publisher
  26. Babu R, Brown A. A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages. Retrovirology. 2013;10:155 pubmed publisher
  27. Preza G, Tanner K, Elliott J, Yang O, Anton P, Ochoa M. Antigen-presenting cell candidates for HIV-1 transmission in human distal colonic mucosa defined by CD207 dendritic cells and CD209 macrophages. AIDS Res Hum Retroviruses. 2014;30:241-9 pubmed publisher
  28. Kristóf E, Zahuczky G, Katona K, Doró Z, Nagy E, Fesus L. Novel role of ICAM3 and LFA-1 in the clearance of apoptotic neutrophils by human macrophages. Apoptosis. 2013;18:1235-51 pubmed publisher
  29. Piercey Åkesson C, Press C, Tranulis M, Jeffrey M, Aleksandersen M, Landsverk T, et al. Phenotypic characterization of cells participating in transport of prion protein aggregates across the intestinal mucosa of sheep. Prion. 2012;6:261-75 pubmed publisher
  30. Fung E, Esposito L, Todd J, Wicker L. Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry. Nat Protoc. 2010;5:357-70 pubmed publisher
  31. Laudanski K, De A, Miller Graziano C. Exogenous heat shock protein 27 uniquely blocks differentiation of monocytes to dendritic cells. Eur J Immunol. 2007;37:2812-24 pubmed