This is a Validated Antibody Database (VAD) review about human DAP12, based on 6 published articles (read how Labome selects the articles), using DAP12 antibody in all methods. It is aimed to help Labome visitors find the most suited DAP12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DAP12 synonym: DAP12; KARAP; PLOSL; TYRO protein tyrosine kinase-binding protein; DNAX-activation protein 12; KAR-associated protein; killer-activating receptor-associated protein

Knockout validation
Cell Signaling Technology
rabbit monoclonal (D7G1X)
  • western blot knockout validation; mouse; loading ...; fig e8a
In order to investigate the function of SLAMF in phagocytosis of haematopoietic tumour cells, Cell Signaling Technology DAP12 antibody (New England Biolabs, D7G1X) was used in western blot knockout validation on mouse samples (fig e8a). Nature (2017) ncbi
Abcam
rabbit monoclonal (EPR5173)
  • western blot; human; 1:300; loading ...; fig 7i
Abcam DAP12 antibody (Abcam, ab124834) was used in western blot on human samples at 1:300 (fig 7i). Cell Mol Gastroenterol Hepatol (2018) ncbi
rabbit monoclonal (EPR5173)
  • immunohistochemistry - free floating section; human; 1:500
  • western blot; human; 1:500
In order to correlate TREM2 expression with neurodegenerative markers in Alzheimer's disease, Abcam DAP12 antibody (Abcam, ab124834) was used in immunohistochemistry - free floating section on human samples at 1:500 and in western blot on human samples at 1:500. Brain Pathol (2015) ncbi
R&D Systems
mouse monoclonal (406288)
  • flow cytometry; human; loading ...; fig s1a
In order to investigate cross-talk between Alzheimer loci, R&D Systems DAP12 antibody (R&D, 406288) was used in flow cytometry on human samples (fig s1a). Nat Neurosci (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D7G1X)
  • immunoprecipitation; mouse; loading ...; fig s3c
Cell Signaling Technology DAP12 antibody (Cell Signaling Technology, 12492) was used in immunoprecipitation on mouse samples (fig s3c). Neuron (2018) ncbi
rabbit monoclonal (D7G1X)
  • western blot knockout validation; mouse; loading ...; fig e8a
In order to investigate the function of SLAMF in phagocytosis of haematopoietic tumour cells, Cell Signaling Technology DAP12 antibody (New England Biolabs, D7G1X) was used in western blot knockout validation on mouse samples (fig e8a). Nature (2017) ncbi
Beckman Coulter
rat monoclonal (H10E12F4)
  • blocking or activating experiments; human; fig 7c
In order to assess the effects of platelet-derived ectosomes on natural killer cells, Beckman Coulter DAP12 antibody (Beckman Coulter, H10E12F4) was used in blocking or activating experiments on human samples (fig 7c). J Immunol (2016) ncbi
Articles Reviewed
  1. Duggan S, Garry C, Behan F, Phipps S, Kudo H, Kirca M, et al. siRNA Library Screening Identifies a Druggable Immune-Signature Driving Esophageal Adenocarcinoma Cell Growth. Cell Mol Gastroenterol Hepatol. 2018;5:569-590 pubmed publisher
  2. Zhao Y, Wu X, Li X, Jiang L, Gui X, Liu Y, et al. TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function. Neuron. 2018;97:1023-1031.e7 pubmed publisher
  3. Chen J, Zhong M, Guo H, Davidson D, Mishel S, Lu Y, et al. SLAMF7 is critical for phagocytosis of haematopoietic tumour cells via Mac-1 integrin. Nature. 2017;544:493-497 pubmed publisher
  4. Sadallah S, Schmied L, Eken C, Charoudeh H, Amicarella F, Schifferli J. Platelet-Derived Ectosomes Reduce NK Cell Function. J Immunol. 2016;197:1663-71 pubmed publisher
  5. Chan G, White C, Winn P, Cimpean M, Replogle J, Glick L, et al. CD33 modulates TREM2: convergence of Alzheimer loci. Nat Neurosci. 2015;18:1556-8 pubmed publisher
  6. Lue L, Schmitz C, Serrano G, Sue L, Beach T, Walker D. TREM2 Protein Expression Changes Correlate with Alzheimer's Disease Neurodegenerative Pathologies in Post-Mortem Temporal Cortices. Brain Pathol. 2015;25:469-80 pubmed publisher