This is a Validated Antibody Database (VAD) review about human Cul2, based on 11 published articles (read how Labome selects the articles), using Cul2 antibody in all methods. It is aimed to help Labome visitors find the most suited Cul2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Cul2 synonym: cullin-2; CUL-2; testis secretory sperm-binding protein Li 238E

Santa Cruz Biotechnology
mouse monoclonal (C-4)
  • western blot; human; 1 ug/ml; loading ...; fig 1E
Santa Cruz Biotechnology Cul2 antibody (Santa cruz, sc-166506) was used in western blot on human samples at 1 ug/ml (fig 1E). J Cell Biol (2016) ncbi
mouse monoclonal (C-4)
  • western blot; human; loading ...; fig 7a
Santa Cruz Biotechnology Cul2 antibody (Santa Cruz, sc-166506) was used in western blot on human samples (fig 7a). Viruses (2016) ncbi
mouse monoclonal (C-4)
  • western blot; human; fig 4a
Santa Cruz Biotechnology Cul2 antibody (Santa Cruz, sc-166506) was used in western blot on human samples (fig 4a). Mol Cell Biol (2016) ncbi
mouse monoclonal (C-4)
  • immunoprecipitation; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 4b
Santa Cruz Biotechnology Cul2 antibody (Santa Cruz, SC-166506) was used in immunoprecipitation on human samples (fig 4b) and in western blot on human samples (fig 4b). J Cell Sci (2014) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 2a
In order to show that expression of von Hippel-Lindau-anti-green fluorescence protein-affinity-directed protein missile results in near-complete degradation of endogenous GFP-VPS34 and PAWS1-GFP, Invitrogen Cul2 antibody (Invitrogen, 51-1800) was used in western blot on human samples at 1:1000 (fig 2a). Open Biol (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 6s1
In order to study the cullin-RING ubiquitin ligase deneddylation cycle and COP9-signalosome activation by structural and kinetic analysis, Invitrogen Cul2 antibody (Thermo Scientific, 51-1800) was used in western blot on human samples (fig 6s1). elife (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 2
In order to show that NEDD8 links CRL ubiquitin ligase function to the p97 pathway, Invitrogen Cul2 antibody (Zymed, 51-1800) was used in western blot on human samples (fig 2). Nat Struct Mol Biol (2012) ncbi
rabbit polyclonal
  • western blot; human; fig 2
In order to elucidate the mechanism through which COP9 signalosome promotes cullin RING ligase activity in vivo, Invitrogen Cul2 antibody (Zymed, 51-1800) was used in western blot on human samples (fig 2). Mol Biol Cell (2011) ncbi
rabbit polyclonal
  • western blot; human; fig 1
In order to study the effect of neddylation on Cul2 and Cul3 structure, Invitrogen Cul2 antibody (Zymed, 51-1800) was used in western blot on human samples (fig 1). J Mol Biol (2011) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; loading ...; fig s3b
In order to determine the rotavirus NSP1-host protein interaction network, Bethyl Cul2 antibody (Bethyl Lab, A302-476A) was used in western blot on human samples (fig s3b). PLoS Pathog (2016) ncbi
Abcam
rabbit monoclonal (EPR3104(2))
  • western blot; human; 1:500; fig 4
Abcam Cul2 antibody (Abcam, ab166917) was used in western blot on human samples at 1:500 (fig 4). J Cell Sci (2016) ncbi
Articles Reviewed
  1. Fulcher L, MacArtney T, Bozatzi P, Hornberger A, Rojas Fernandez A, Sapkota G. An affinity-directed protein missile system for targeted proteolysis. Open Biol. 2016;6: pubmed
  2. Ding S, Mooney N, Li B, Kelly M, Feng N, Loktev A, et al. Comparative Proteomics Reveals Strain-Specific ?-TrCP Degradation via Rotavirus NSP1 Hijacking a Host Cullin-3-Rbx1 Complex. PLoS Pathog. 2016;12:e1005929 pubmed publisher
  3. Uematsu K, Okumura F, Tonogai S, Joo Okumura A, Alemayehu D, Nishikimi A, et al. ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation. J Cell Biol. 2016;215:95-106 pubmed
  4. Devadas K, Biswas S, Haleyurgirisetty M, Ragupathy V, Wang X, Lee S, et al. Identification of Host Micro RNAs That Differentiate HIV-1 and HIV-2 Infection Using Genome Expression Profiling Techniques. Viruses. 2016;8: pubmed publisher
  5. Mosadeghi R, Reichermeier K, Winkler M, Schreiber A, Reitsma J, Zhang Y, et al. Structural and kinetic analysis of the COP9-Signalosome activation and the cullin-RING ubiquitin ligase deneddylation cycle. elife. 2016;5: pubmed publisher
  6. Chen K, Zeng J, Xiao H, Huang C, Hu J, Yao W, et al. Regulation of glucose metabolism by p62/SQSTM1 through HIF1α. J Cell Sci. 2016;129:817-30 pubmed publisher
  7. Matsunuma R, Niida H, Ohhata T, Kitagawa K, Sakai S, Uchida C, et al. UV Damage-Induced Phosphorylation of HBO1 Triggers CRL4DDB2-Mediated Degradation To Regulate Cell Proliferation. Mol Cell Biol. 2016;36:394-406 pubmed publisher
  8. Levay K, Slepak V. Regulation of Cop9 signalosome activity by the EF-hand Ca2+-binding protein tescalcin. J Cell Sci. 2014;127:2448-59 pubmed publisher
  9. den Besten W, Verma R, Kleiger G, Oania R, Deshaies R. NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway. Nat Struct Mol Biol. 2012;19:511-6, S1 pubmed publisher
  10. Choo Y, Boh B, Lou J, Eng J, Leck Y, Anders B, et al. Characterization of the role of COP9 signalosome in regulating cullin E3 ubiquitin ligase activity. Mol Biol Cell. 2011;22:4706-15 pubmed publisher
  11. Boh B, Smith P, Hagen T. Neddylation-induced conformational control regulates cullin RING ligase activity in vivo. J Mol Biol. 2011;409:136-45 pubmed publisher