This is a Validated Antibody Database (VAD) review about human Cul1, based on 31 published articles (read how Labome selects the articles), using Cul1 antibody in all methods. It is aimed to help Labome visitors find the most suited Cul1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Santa Cruz Biotechnology
mouse monoclonal (D-5)
  • western blot; human; loading ...; fig 1b
Santa Cruz Biotechnology Cul1 antibody (Santa, sc-17775) was used in western blot on human samples (fig 1b). J Pathol (2019) ncbi
mouse monoclonal (D-5)
  • western blot; human; 1:1000; loading ...; fig s4b
Santa Cruz Biotechnology Cul1 antibody (Santa Cruz, sc-17775) was used in western blot on human samples at 1:1000 (fig s4b). Nat Commun (2018) ncbi
mouse monoclonal (AS97)
  • western blot; human; loading ...; fig 1e
Santa Cruz Biotechnology Cul1 antibody (Santa, sc-12761) was used in western blot on human samples (fig 1e). Science (2018) ncbi
mouse monoclonal (D-5)
  • western blot; human; loading ...; fig 8c
Santa Cruz Biotechnology Cul1 antibody (SantaCruz, sc-17775) was used in western blot on human samples (fig 8c). J Virol (2018) ncbi
mouse monoclonal (D-5)
  • western blot; human; loading ...; fig 5b
Santa Cruz Biotechnology Cul1 antibody (Santa, sc-17775) was used in western blot on human samples (fig 5b). Nat Chem Biol (2017) ncbi
mouse monoclonal (D-5)
  • western blot; human; 1:500; fig s2
Santa Cruz Biotechnology Cul1 antibody (Santa Cruz, sc-17775) was used in western blot on human samples at 1:500 (fig s2). EMBO J (2016) ncbi
mouse monoclonal (D-5)
  • western blot; human; loading ...; fig 1a
In order to investigate the phosphorylation and ubiquitylation of autoimmune regulator, Santa Cruz Biotechnology Cul1 antibody (Santa Cruz, sc-17775) was used in western blot on human samples (fig 1a). J Biol Chem (2016) ncbi
mouse monoclonal (D-5)
  • western blot; mouse; loading ...; fig s4d
Santa Cruz Biotechnology Cul1 antibody (Santa Cruz, sc-17775) was used in western blot on mouse samples (fig s4d). Nature (2016) ncbi
mouse monoclonal (D-5)
  • western blot; human; fig 6
In order to study the cullin-RING ubiquitin ligase deneddylation cycle and COP9-signalosome activation by structural and kinetic analysis, Santa Cruz Biotechnology Cul1 antibody (Santa Cruz Biotechnology, sc-17775) was used in western blot on human samples (fig 6). elife (2016) ncbi
mouse monoclonal (D-5)
  • western blot; human; fig 4a
Santa Cruz Biotechnology Cul1 antibody (Santa Cruz, sc-17775) was used in western blot on human samples (fig 4a). Mol Cell Biol (2016) ncbi
mouse monoclonal (D-5)
  • western blot; human; loading ...; fig 2b
Santa Cruz Biotechnology Cul1 antibody (Santa Cruz, sc-17775) was used in western blot on human samples (fig 2b). Nucleic Acids Res (2014) ncbi
mouse monoclonal (D-5)
  • western blot; human
Santa Cruz Biotechnology Cul1 antibody (Santa Cruz, SC-17775) was used in western blot on human samples . FEBS Lett (2014) ncbi
mouse monoclonal (D-5)
  • immunoprecipitation; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 4b
Santa Cruz Biotechnology Cul1 antibody (Santa Cruz, sc-17775) was used in immunoprecipitation on human samples (fig 4b) and in western blot on human samples (fig 4b). J Cell Sci (2014) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; human; loading ...; fig s1b
In order to understand the interaction between PTEN and FBXL2 in the context of apoptosis, Invitrogen Cul1 antibody (Invitrogen, 718700) was used in western blot on human samples (fig s1b). Nature (2017) ncbi
mouse monoclonal (2H4C9)
  • western blot; human; loading ...; fig s10
In order to identify compounds that inhibit the proteasome 19S subunit Rpn11, Invitrogen Cul1 antibody (Life Technologies, 32-2400) was used in western blot on human samples (fig s10). Nat Chem Biol (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 1e
In order to identify and characterize an inhibitor of the COP9 signalosome, Invitrogen Cul1 antibody (Invitrogen, 718700) was used in western blot on human samples at 1:500 (fig 1e). Nat Commun (2016) ncbi
mouse monoclonal (2H4C9)
  • western blot; human; 1:500; fig 1
In order to investigate stabilization of XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma via USP9X, Invitrogen Cul1 antibody (Invitrogen, 32-2400) was used in western blot on human samples at 1:500 (fig 1). EMBO Mol Med (2016) ncbi
mouse monoclonal (2H4C9)
  • western blot; mouse; 1:500; fig s4
  • western blot; human; 1:500; fig 1
In order to study disruption of the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity due to immunomodulatory drugs, Invitrogen Cul1 antibody (Invitrogen, 32-2400) was used in western blot on mouse samples at 1:500 (fig s4) and in western blot on human samples at 1:500 (fig 1). Nat Med (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 1
In order to analyze the DCN-type NEDD8 E3 ligase mammalian family, Invitrogen Cul1 antibody (Life Technologies, 718700) was used in western blot on human samples at 1:1000 (fig 1). J Cell Sci (2016) ncbi
mouse monoclonal (2H4C9)
  • western blot; human; fig 3
In order to utilize tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps) to isolate ubiquitinated substrates, Invitrogen Cul1 antibody (Invitrogen, 32-2400) was used in western blot on human samples (fig 3). Nat Protoc (2016) ncbi
mouse monoclonal (2H4C9)
  • western blot; human; fig 5
In order to analyze recognition by an SCF E3 ligase complex containing the orphan F-box protein FBXO21 by peptidic degron in EID1, Invitrogen Cul1 antibody (Invitrogen, 322400) was used in western blot on human samples (fig 5). Proc Natl Acad Sci U S A (2015) ncbi
mouse monoclonal (2H4C9)
  • immunoprecipitation; human; 5 ug; fig 2
In order to examine the localization and function of KIAA1199 in human colorectal tumors, Invitrogen Cul1 antibody (Invitrogen, 32-2400) was used in immunoprecipitation on human samples at 5 ug (fig 2). PLoS ONE (2013) ncbi
mouse monoclonal (2H4C9)
  • western blot; human; 1:1000; fig 3
In order to analyze UPR signaling and its role in small molecule-induced mitochondrial disruption and prostate cancer inhibition, Invitrogen Cul1 antibody (Zymed, 32-2400) was used in western blot on human samples at 1:1000 (fig 3). Oncotarget (2013) ncbi
mouse monoclonal (2H4C9)
  • western blot; human; fig 1
In order to study the mechanism by which the tumor-suppressor SCF(FBW7) regulates cellular apoptosis, Invitrogen Cul1 antibody (Invitrogen, 32-2400) was used in western blot on human samples (fig 1). Nature (2011) ncbi
mouse monoclonal (2H4C9)
  • western blot; human; fig s5
In order to investigate the interaction between REST and beta-TrCP, Invitrogen Cul1 antibody (Zymed, 32-2400) was used in western blot on human samples (fig s5). Nature (2008) ncbi
Abcam
domestic rabbit monoclonal (EPR3103Y)
  • western blot; human; 1:200; loading ...; fig 6g
Abcam Cul1 antibody (Abcam, ab75817) was used in western blot on human samples at 1:200 (fig 6g). Nat Commun (2019) ncbi
domestic rabbit monoclonal (EPR3103Y)
  • western blot; mouse; loading ...
Abcam Cul1 antibody (Abcam, ab75817) was used in western blot on mouse samples . elife (2016) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
In order to study the role of Skp2 in paclitaxel-resistant prostate cancer cells, MilliporeSigma Cul1 antibody (Sigma, C7117) was used in western blot on human samples (fig 2a). Oncol Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2b
In order to understand the function of FBXO32 in inflammatory and genotoxic stress, Cell Signaling Technology Cul1 antibody (Cell Signaling Technologies, 4995) was used in western blot on human samples (fig 2b). Int J Biochem Cell Biol (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2b
In order to determine the rotavirus NSP1-host protein interaction network, Cell Signaling Technology Cul1 antibody (CST, 4995) was used in western blot on human samples (fig 2b). PLoS Pathog (2016) ncbi
BD Biosciences
mouse monoclonal (19/CUL-1)
  • western blot; human; 1:300; loading ...; fig 2i
BD Biosciences Cul1 antibody (BD Biosciences, 612040) was used in western blot on human samples at 1:300 (fig 2i). Nat Commun (2018) ncbi
Articles Reviewed
  1. Paul D, Islam S, Manne R, Dinesh U, Malonia S, Maity B, et al. F-box protein FBXO16 functions as a tumor suppressor by attenuating nuclear β-catenin function. J Pathol. 2019;248:266-279 pubmed publisher
  2. Ji Q, Xu X, Kang L, Xu Y, Xiao J, Goodman S, et al. Hematopoietic PBX-interacting protein mediates cartilage degeneration during the pathogenesis of osteoarthritis. Nat Commun. 2019;10:313 pubmed publisher
  3. Li Y, Liu Y, Xu H, Jiang G, Van der Jeught K, Fang Y, et al. Heterozygous deletion of chromosome 17p renders prostate cancer vulnerable to inhibition of RNA polymerase II. Nat Commun. 2018;9:4394 pubmed publisher
  4. Mena E, Kjolby R, Saxton R, Werner A, Lew B, Boyle J, et al. Dimerization quality control ensures neuronal development and survival. Science. 2018;362: pubmed publisher
  5. Yao F, Zhou Z, Kim J, Hang Q, Xiao Z, Ton B, et al. SKP2- and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity. Nat Commun. 2018;9:2269 pubmed publisher
  6. Liu R, Moss B. Vaccinia Virus C9 Ankyrin Repeat/F-Box Protein Is a Newly Identified Antagonist of the Type I Interferon-Induced Antiviral State. J Virol. 2018;92: pubmed publisher
  7. Meshram S, Paul D, Manne R, Choppara S, Sankaran G, Agrawal Y, et al. FBXO32 activates NF-κB through IκBα degradation in inflammatory and genotoxic stress. Int J Biochem Cell Biol. 2017;92:134-140 pubmed publisher
  8. Kuchay S, Giorgi C, Simoneschi D, Pagan J, Missiroli S, Saraf A, et al. PTEN counteracts FBXL2 to promote IP3R3- and Ca2+-mediated apoptosis limiting tumour growth. Nature. 2017;546:554-558 pubmed publisher
  9. Scott D, Hammill J, Min J, Rhee D, Connelly M, Sviderskiy V, et al. Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase. Nat Chem Biol. 2017;13:850-857 pubmed publisher
  10. Li J, Yakushi T, Parlati F, MacKinnon A, Pérez C, Ma Y, et al. Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11. Nat Chem Biol. 2017;13:486-493 pubmed publisher
  11. Schlierf A, Altmann E, Quancard J, Jefferson A, Assenberg R, Renatus M, et al. Targeted inhibition of the COP9 signalosome for treatment of cancer. Nat Commun. 2016;7:13166 pubmed publisher
  12. Ding S, Mooney N, Li B, Kelly M, Feng N, Loktev A, et al. Comparative Proteomics Reveals Strain-Specific β-TrCP Degradation via Rotavirus NSP1 Hijacking a Host Cullin-3-Rbx1 Complex. PLoS Pathog. 2016;12:e1005929 pubmed publisher
  13. Vingill S, Brockelt D, Lancelin C, Tatenhorst L, Dontcheva G, Preisinger C, et al. Loss of FBXO7 (PARK15) results in reduced proteasome activity and models a parkinsonism-like phenotype in mice. EMBO J. 2016;35:2008-25 pubmed publisher
  14. Shao W, Zumer K, Fujinaga K, Peterlin B. FBXO3 Protein Promotes Ubiquitylation and Transcriptional Activity of AIRE (Autoimmune Regulator). J Biol Chem. 2016;291:17953-63 pubmed publisher
  15. Engel K, Rudelius M, Slawska J, Jacobs L, Ahangarian Abhari B, Altmann B, et al. USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma. EMBO Mol Med. 2016;8:851-62 pubmed publisher
  16. Shin H, Kim H, Oh S, Lee J, Kee M, Ko H, et al. AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy. Nature. 2016;534:553-7 pubmed publisher
  17. Eichner R, Heider M, Fernández Sáiz V, van Bebber F, Garz A, Lemeer S, et al. Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity. Nat Med. 2016;22:735-43 pubmed publisher
  18. Yang Y, Lu Y, Wang L, Mizokami A, Keller E, Zhang J, et al. Skp2 is associated with paclitaxel resistance in prostate cancer cells. Oncol Rep. 2016;36:559-66 pubmed publisher
  19. Yu Z, Chen T, Li X, Yang M, Tang S, Zhu X, et al. Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response. elife. 2016;5: pubmed publisher
  20. Mosadeghi R, Reichermeier K, Winkler M, Schreiber A, Reitsma J, Zhang Y, et al. Structural and kinetic analysis of the COP9-Signalosome activation and the cullin-RING ubiquitin ligase deneddylation cycle. elife. 2016;5: pubmed publisher
  21. Keuss M, Thomas Y, Mcarthur R, Wood N, Knebel A, Kurz T. Characterization of the mammalian family of DCN-type NEDD8 E3 ligases. J Cell Sci. 2016;129:1441-54 pubmed publisher
  22. Mark K, Loveless T, Toczyski D. Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps). Nat Protoc. 2016;11:291-301 pubmed publisher
  23. Zhang C, Li X, Adelmant G, Dobbins J, Geisen C, Oser M, et al. Peptidic degron in EID1 is recognized by an SCF E3 ligase complex containing the orphan F-box protein FBXO21. Proc Natl Acad Sci U S A. 2015;112:15372-7 pubmed publisher
  24. Matsunuma R, Niida H, Ohhata T, Kitagawa K, Sakai S, Uchida C, et al. UV Damage-Induced Phosphorylation of HBO1 Triggers CRL4DDB2-Mediated Degradation To Regulate Cell Proliferation. Mol Cell Biol. 2016;36:394-406 pubmed publisher
  25. Chen Z, Liu B, Tang N, Xu Y, Ye X, Li Z, et al. FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Res. 2014;42:11560-9 pubmed publisher
  26. Li S, Song W, Jiang M, Zeng L, Zhu X, Chen J. Phosphorylation of cyclin Y by CDK14 induces its ubiquitination and degradation. FEBS Lett. 2014;588:1989-96 pubmed publisher
  27. Levay K, Slepak V. Regulation of Cop9 signalosome activity by the EF-hand Ca2+-binding protein tescalcin. J Cell Sci. 2014;127:2448-59 pubmed publisher
  28. Tiwari A, Schneider M, Fiorino A, Haider R, Okoniewski M, Roschitzki B, et al. Early insights into the function of KIAA1199, a markedly overexpressed protein in human colorectal tumors. PLoS ONE. 2013;8:e69473 pubmed publisher
  29. Rico Bautista E, Zhu W, Kitada S, Ganapathy S, Lau E, Krajewski S, et al. Small molecule-induced mitochondrial disruption directs prostate cancer inhibition via UPR signaling. Oncotarget. 2013;4:1212-29 pubmed
  30. Inuzuka H, Shaik S, Onoyama I, Gao D, Tseng A, Maser R, et al. SCF(FBW7) regulates cellular apoptosis by targeting MCL1 for ubiquitylation and destruction. Nature. 2011;471:104-9 pubmed publisher
  31. Guardavaccaro D, Frescas D, Dorrello N, Peschiaroli A, Multani A, Cardozo T, et al. Control of chromosome stability by the beta-TrCP-REST-Mad2 axis. Nature. 2008;452:365-9 pubmed publisher