This is a Validated Antibody Database (VAD) review about human CYP3A4, based on 20 published articles (read how Labome selects the articles), using CYP3A4 antibody in all methods. It is aimed to help Labome visitors find the most suited CYP3A4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CYP3A4 synonym: CP33; CP34; CYP3A; CYP3A3; CYPIIIA3; CYPIIIA4; HLP; NF-25; P450C3; P450PCN1

Abcam
domestic rabbit monoclonal (EPR6202)
  • immunohistochemistry; human; 1:300; loading ...; fig 4a
Abcam CYP3A4 antibody (Abcam, EPR6202) was used in immunohistochemistry on human samples at 1:300 (fig 4a). Sci Rep (2022) ncbi
domestic rabbit monoclonal (EPR6202)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1c
Abcam CYP3A4 antibody (Abcam, EPR6202) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1c). Cancers (Basel) (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:2000; loading ...; fig 5b
Abcam CYP3A4 antibody (Abcam, ab3572) was used in western blot on mouse samples at 1:2000 (fig 5b). Arch Toxicol (2021) ncbi
domestic rabbit monoclonal (EPR6202)
  • immunohistochemistry; common marmoset; fig 6
Abcam CYP3A4 antibody (Abcam, ab124921) was used in immunohistochemistry on common marmoset samples (fig 6). Hepat Med (2020) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:200; loading ...; fig 6s1d
Abcam CYP3A4 antibody (Abcam, ab3572) was used in immunocytochemistry on human samples at 1:200 (fig 6s1d). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3b
Abcam CYP3A4 antibody (Abcam, ab3572) was used in western blot on human samples at 1:1000 (fig 3b). Front Pharmacol (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; rat; 1:100; loading ...; fig 5a
Abcam CYP3A4 antibody (Abcam, ab3572) was used in immunohistochemistry on rat samples at 1:100 (fig 5a). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; rat; 1:70; fig 2c
  • western blot; rat; 1:1000; fig 2b
In order to develop a method to increase the intestinal transport of octreotide, Abcam CYP3A4 antibody (Abcam, ab3572) was used in immunohistochemistry - frozen section on rat samples at 1:70 (fig 2c) and in western blot on rat samples at 1:1000 (fig 2b). Exp Ther Med (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:100; loading ...; tbl 1
In order to examine the role of dipeptidyl-peptidase IV in age-dependent liver repopulation by transplanted hepatocytes, Abcam CYP3A4 antibody (Abcam, ab3572) was used in immunohistochemistry - paraffin section on rat samples at 1:100 (tbl 1). Am J Pathol (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (HL3)
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 2
Santa Cruz Biotechnology CYP3A4 antibody (Santa Cruz, sc-53850) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 2). Front Immunol (2021) ncbi
mouse monoclonal (HL3)
  • western blot; mouse; loading ...; fig 2a
Santa Cruz Biotechnology CYP3A4 antibody (Santa, sc-53850) was used in western blot on mouse samples (fig 2a). Drug Metab Dispos (2020) ncbi
mouse monoclonal (HL3)
  • western blot; human; loading ...; fig 3d
Santa Cruz Biotechnology CYP3A4 antibody (Santa, HL3) was used in western blot on human samples (fig 3d). Carcinogenesis (2017) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig 3d
Santa Cruz Biotechnology CYP3A4 antibody (Santa, HL3) was used in western blot on human samples (fig 3d). Carcinogenesis (2017) ncbi
mouse monoclonal (P6)
  • western blot; mouse; 1:500; loading ...; fig 10a
Santa Cruz Biotechnology CYP3A4 antibody (SantaCruz, sc-53246) was used in western blot on mouse samples at 1:500 (fig 10a). Drug Metab Dispos (2017) ncbi
mouse monoclonal (HL3)
  • western blot; human; loading ...; fig 6c
Santa Cruz Biotechnology CYP3A4 antibody (Santa Cruz, sc-53850) was used in western blot on human samples (fig 6c). Sci Rep (2016) ncbi
mouse monoclonal (HL3)
  • western blot; human; loading ...; fig 6b
Santa Cruz Biotechnology CYP3A4 antibody (Santa Cruz, sc-53850) was used in western blot on human samples (fig 6b). Toxicol Lett (2016) ncbi
mouse monoclonal (HL3)
  • western blot; human; fig 6
In order to determine facilitation of chemoresistance and cancer proliferation by Haem-dependent dimerization of PGRMC1/Sigma-2 receptor, Santa Cruz Biotechnology CYP3A4 antibody (Santa Cruz, sc-53850) was used in western blot on human samples (fig 6). Nat Commun (2016) ncbi
mouse monoclonal (P6)
  • western blot; rat; 1:500
Santa Cruz Biotechnology CYP3A4 antibody (Santa Cruz, sc-53246) was used in western blot on rat samples at 1:500. Toxicol In Vitro (2015) ncbi
mouse monoclonal (HL3)
  • western blot; human; 1:2000
Santa Cruz Biotechnology CYP3A4 antibody (Santa Cruz Biotechnology, sc-53850) was used in western blot on human samples at 1:2000. Arch Toxicol (2014) ncbi
Abnova
mouse polyclonal
Abnova CYP3A4 antibody (Abnova, H00001576-B01P) was used . J Clin Transl Hepatol (2021) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1g
In order to test the toxicity of The gamma-hydroxybutyric acid receptor antagonist NCS-382 in HepG2 and primary hepatocyte cells, Invitrogen CYP3A4 antibody (ThermoFisher, PA1-343) was used in western blot on human samples (fig 1g). Toxicol In Vitro (2017) ncbi
Articles Reviewed
  1. Uehara S, Iida Y, Ida Tanaka M, Goto M, Kawai K, Yamamoto M, et al. Humanized liver TK-NOG mice with functional deletion of hepatic murine cytochrome P450s as a model for studying human drug metabolism. Sci Rep. 2022;12:14907 pubmed publisher
  2. Niu Y, Fan S, Luo Q, Chen L, Huang D, Chang W, et al. Interaction of Hepatitis B Virus X Protein with the Pregnane X Receptor Enhances the Synergistic Effects of Aflatoxin B1 and Hepatitis B Virus on Promoting Hepatocarcinogenesis. J Clin Transl Hepatol. 2021;9:466-476 pubmed publisher
  3. Taavela J, Viiri K, Välimäki A, Sarin J, Salonoja K, Maki M, et al. Apolipoprotein A4 Defines the Villus-Crypt Border in Duodenal Specimens for Celiac Disease Morphometry. Front Immunol. 2021;12:713854 pubmed publisher
  4. Matheux A, Gassiot M, Fromont G, Leenhardt F, Boulahtouf A, Fabbrizio E, et al. PXR Modulates the Prostate Cancer Cell Response to Afatinib by Regulating the Expression of the Monocarboxylate Transporter SLC16A1. Cancers (Basel). 2021;13: pubmed publisher
  5. Howell L, Jenkins R, Lynch S, Duckworth C, Kevin Park B, Goldring C. Proteomic profiling of murine biliary-derived hepatic organoids and their capacity for drug disposition, bioactivation and detoxification. Arch Toxicol. 2021;95:2413-2430 pubmed publisher
  6. Jiao T, Yao X, Zhao Y, Zhou Y, Gao Y, Fan S, et al. Dexamethasone-Induced Liver Enlargement Is Related to PXR/YAP Activation and Lipid Accumulation but Not Hepatocyte Proliferation. Drug Metab Dispos. 2020;48:830-839 pubmed publisher
  7. Aravalli R, COLLINS D, Hapke J, Crane A, Steer C. Hepatic Differentiation of Marmoset Embryonic Stem Cells and Functional Characterization of ESC-Derived Hepatocyte-Like Cells. Hepat Med. 2020;12:15-27 pubmed publisher
  8. Katsuda T, Matsuzaki J, Yamaguchi T, Yamada Y, Prieto Vila M, Hosaka K, et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. elife. 2019;8: pubmed publisher
  9. Valanejad L, Lewis K, Wright M, Jiang Y, D Souza A, Karns R, et al. FXR-Gankyrin axis is involved in development of pediatric liver cancer. Carcinogenesis. 2017;38:738-747 pubmed publisher
  10. Okada N, Murakami A, Urushizaki S, Matsuda M, Kawazoe K, Ishizawa K. Extracts of Immature Orange (Aurantii fructus immaturus) and Citrus Unshiu Peel (Citri unshiu pericarpium) Induce P-Glycoprotein and Cytochrome P450 3A4 Expression via Upregulation of Pregnane X Receptor. Front Pharmacol. 2017;8:84 pubmed publisher
  11. Cheng S, Bammler T, Cui J. RNA Sequencing Reveals Age and Species Differences of Constitutive Androstane Receptor-Targeted Drug-Processing Genes in the Liver. Drug Metab Dispos. 2017;45:867-882 pubmed publisher
  12. Lu J, Shao Y, Qin X, Liu D, Chen A, Li D, et al. CRISPR knockout rat cytochrome P450 3A1/2 model for advancing drug metabolism and pharmacokinetics research. Sci Rep. 2017;7:42922 pubmed publisher
  13. Vogel K, Ainslie G, Roullet J, McConnell A, Gibson K. In vitro toxicological evaluation of NCS-382, a high-affinity antagonist of ?-hydroxybutyrate (GHB) binding. Toxicol In Vitro. 2017;40:196-202 pubmed publisher
  14. Sun X, Duan Z, Liu Z, Tang S, Li Y, He S, et al. Inhibition of P-glycoprotein, multidrug resistance-associated protein 2 and cytochrome P450 3A4 improves the oral absorption of octreotide in rats with portal hypertension. Exp Ther Med. 2016;12:3716-3722 pubmed publisher
  15. Stock P, Bielohuby M, Staege M, Hsu M, Bidlingmaier M, Christ B. Impairment of Host Liver Repopulation by Transplanted Hepatocytes in Aged Rats and the Release by Short-Term Growth Hormone Treatment. Am J Pathol. 2017;187:553-569 pubmed publisher
  16. Milkiewicz M, Klak M, Kempińska Podhorodecka A, Wiechowska Kozlowska A, Urasinska E, Blatkiewicz M, et al. Impaired Hepatic Adaptation to Chronic Cholestasis induced by Primary Sclerosing Cholangitis. Sci Rep. 2016;6:39573 pubmed publisher
  17. Štěpánková M, Krasulová K, Doricakova A, Kurka O, Anzenbacher P, Dvorak Z. Optical isomers of dihydropyridine calcium channel blockers display enantiospecific effects on the expression and enzyme activities of human xenobiotics-metabolizing cytochromes P450. Toxicol Lett. 2016;262:173-186 pubmed publisher
  18. Kabe Y, Nakane T, Koike I, Yamamoto T, Sugiura Y, Harada E, et al. Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance. Nat Commun. 2016;7:11030 pubmed publisher
  19. Vichi S, Sandström von Tobel J, Gemma S, Stanzel S, Kopp Schneider A, Monnet Tschudi F, et al. Cell type-specific expression and localization of cytochrome P450 isoforms in tridimensional aggregating rat brain cell cultures. Toxicol In Vitro. 2015;30:176-84 pubmed publisher
  20. Smutny T, Bitman M, Urban M, Dubecka M, Vrzal R, Dvorak Z, et al. U0126, a mitogen-activated protein kinase kinase 1 and 2 (MEK1 and 2) inhibitor, selectively up-regulates main isoforms of CYP3A subfamily via a pregnane X receptor (PXR) in HepG2 cells. Arch Toxicol. 2014;88:2243-59 pubmed publisher