This is a Validated Antibody Database (VAD) review about human CYBB, based on 28 published articles (read how Labome selects the articles), using CYBB antibody in all methods. It is aimed to help Labome visitors find the most suited CYBB antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CYBB synonym: AMCBX2; CGD; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (54.1)
  • western blot knockout validation; mouse; loading ...; fig 5c
In order to show that Eros is essential for host defense via the phagocyte NAPDH oxidase, Santa Cruz Biotechnology CYBB antibody (Santa Cruz, sc-130543) was used in western blot knockout validation on mouse samples (fig 5c). J Exp Med (2017) ncbi
Abcam
domestic rabbit monoclonal (EPR6991)
  • western blot; mouse; 1:5000; loading ...; fig 6f
Abcam CYBB antibody (Abcam, ab129068) was used in western blot on mouse samples at 1:5000 (fig 6f). Cell Rep (2022) ncbi
domestic rabbit monoclonal (EPR6991)
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 8i
  • western blot; mouse; 1:1000; loading ...; fig 8g
Abcam CYBB antibody (Abcam, ab129068) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 8i) and in western blot on mouse samples at 1:1000 (fig 8g). Int J Biol Sci (2022) ncbi
domestic rabbit monoclonal (EPR6991)
  • western blot; mouse; 1:200; loading ...; fig s2h
Abcam CYBB antibody (Abcam, Ab129068) was used in western blot on mouse samples at 1:200 (fig s2h). Clin Transl Med (2021) ncbi
domestic rabbit monoclonal (EPR6991)
  • western blot; human; loading ...; fig 7c
Abcam CYBB antibody (Abcam, ab129068) was used in western blot on human samples (fig 7c). Int J Oncol (2018) ncbi
domestic rabbit monoclonal (EPR6991)
  • western blot; rat; loading ...; fig 6
Abcam CYBB antibody (Abcam, ab129068) was used in western blot on rat samples (fig 6). PLoS ONE (2017) ncbi
domestic rabbit monoclonal (EPR6991)
  • western blot; mouse; 1:2500; fig st2
Abcam CYBB antibody (Abcam, ab129068) was used in western blot on mouse samples at 1:2500 (fig st2). PLoS ONE (2016) ncbi
domestic rabbit monoclonal (EPR6991)
  • western blot; human; 1:1000; fig 4
Abcam CYBB antibody (Epitomics, 5653-1) was used in western blot on human samples at 1:1000 (fig 4). Nat Commun (2015) ncbi
domestic rabbit monoclonal (EPR6991)
  • immunoprecipitation; mouse; fig 6
  • immunocytochemistry; mouse; 1:250; fig 6
In order to define transcriptional, metabolic ,and ubiquitin-cycling nodes controlled by Rbpj, Pfkl and Rnf145 during the phagocyte oxidative burst, Abcam CYBB antibody (Abcam, ab129068) was used in immunoprecipitation on mouse samples (fig 6) and in immunocytochemistry on mouse samples at 1:250 (fig 6). Nat Commun (2015) ncbi
domestic rabbit monoclonal (EPR6991)
  • western blot; mouse; 1:1000; fig 5a
In order to assess the anti-fatigue effects of Myelophil, Abcam CYBB antibody (Abcam, ab129068) was used in western blot on mouse samples at 1:1000 (fig 5a). Eur J Pharmacol (2015) ncbi
domestic rabbit monoclonal (EPR6991)
  • western blot; mouse; 1:1000; fig 5
Abcam CYBB antibody (Abcam, 129068) was used in western blot on mouse samples at 1:1000 (fig 5). Cell Stress Chaperones (2015) ncbi
domestic rabbit monoclonal (EPR6991)
  • western blot; human
Abcam CYBB antibody (Epitomics, 5653-1) was used in western blot on human samples . J Neurochem (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (54.1)
  • western blot; rat; loading ...; fig 5a
Santa Cruz Biotechnology CYBB antibody (Santa Cruz Biotechnology, sc-130543) was used in western blot on rat samples (fig 5a). Mol Med Rep (2020) ncbi
mouse monoclonal (54.1)
  • western blot; mouse; loading ...; fig 1c, s5c
  • western blot; human; loading ...; fig s7
Santa Cruz Biotechnology CYBB antibody (Santa Cruz, 54.1) was used in western blot on mouse samples (fig 1c, s5c) and in western blot on human samples (fig s7). Sci Rep (2020) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig 1c, s5c
  • western blot; human; loading ...; fig s7
Santa Cruz Biotechnology CYBB antibody (Santa Cruz, 54.1) was used in western blot on mouse samples (fig 1c, s5c) and in western blot on human samples (fig s7). Sci Rep (2020) ncbi
mouse monoclonal (54.1)
  • western blot knockout validation; mouse; loading ...; fig 5c
In order to show that Eros is essential for host defense via the phagocyte NAPDH oxidase, Santa Cruz Biotechnology CYBB antibody (Santa Cruz, sc-130543) was used in western blot knockout validation on mouse samples (fig 5c). J Exp Med (2017) ncbi
mouse monoclonal (54.1)
  • western blot; human; 1:500; loading ...; fig 4
Santa Cruz Biotechnology CYBB antibody (Santa Cruz, sc-130543) was used in western blot on human samples at 1:500 (fig 4). Mol Med Rep (2017) ncbi
mouse monoclonal (G-1)
  • western blot; human; 1:1000; loading ...; fig 2c
In order to study the role of mitochondrial ferritin protein in iron-dependent cell death (ferroptosis)., Santa Cruz Biotechnology CYBB antibody (Santa Cruz, sc-74514) was used in western blot on human samples at 1:1000 (fig 2c). Front Aging Neurosci (2016) ncbi
mouse monoclonal (G-1)
  • western blot; mouse; loading ...; fig 6a
In order to test if inhibition of the NLRP3 inflammasome prevents inflammatory hyperalgesia induced by lipopolysaccharide, Santa Cruz Biotechnology CYBB antibody (Santa Cruz, SC-74514) was used in western blot on mouse samples (fig 6a). Inflammation (2017) ncbi
mouse monoclonal (54.1)
  • immunohistochemistry; mouse; 1:100; fig 1
  • western blot; mouse; fig 1
Santa Cruz Biotechnology CYBB antibody (Santa Cruz, sc-130543) was used in immunohistochemistry on mouse samples at 1:100 (fig 1) and in western blot on mouse samples (fig 1). Int J Mol Med (2016) ncbi
mouse monoclonal (G-1)
  • western blot; mouse; 1:200; fig 5
Santa Cruz Biotechnology CYBB antibody (Santa Cruz, sc74514) was used in western blot on mouse samples at 1:200 (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (54.1)
  • western blot; human; 1:1000; fig 3
Santa Cruz Biotechnology CYBB antibody (Santa Cruz Biotechnology, sc-130543) was used in western blot on human samples at 1:1000 (fig 3). PLoS ONE (2015) ncbi
mouse monoclonal (G-1)
  • western blot; mouse
In order to study ROS and MAPK signaling in gonadotropes, Santa Cruz Biotechnology CYBB antibody (Santa Cruz Biotechnology, SC-74514) was used in western blot on mouse samples . Endocrinology (2015) ncbi
mouse monoclonal (G-1)
  • western blot; human; 1:200
Santa Cruz Biotechnology CYBB antibody (Santa Cruz, sc-74514) was used in western blot on human samples at 1:200. Biochim Biophys Acta (2015) ncbi
mouse monoclonal (54.1)
  • immunocytochemistry; human; 1:100; loading ...; fig 4b
  • western blot; human; loading ...; fig 3b
In order to examine the effects of rare X-minus chronic granulomatous disease mutations, Santa Cruz Biotechnology CYBB antibody (Santa Cruz, sc-130543) was used in immunocytochemistry on human samples at 1:100 (fig 4b) and in western blot on human samples (fig 3b). Biochem J (2014) ncbi
mouse monoclonal (54.1)
  • western blot; human
Santa Cruz Biotechnology CYBB antibody (Santa Cruz Biotechnology, sc-130543) was used in western blot on human samples . Biochim Biophys Acta (2014) ncbi
mouse monoclonal (NL7)
  • western blot; human
Santa Cruz Biotechnology CYBB antibody (Santa Cruz, sc-130548) was used in western blot on human samples . Free Radic Res (2014) ncbi
Invitrogen
mouse monoclonal (54.1)
  • immunocytochemistry; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 4c
Invitrogen CYBB antibody (Invitrogen, MA5-18052) was used in immunocytochemistry on human samples (fig 4b) and in western blot on human samples (fig 4c). Biomolecules (2021) ncbi
Novus Biologicals
domestic rabbit polyclonal (HIC1-1C10)
  • western blot; rat; loading ...; fig 3f
Novus Biologicals CYBB antibody (Novus Biologicals, NB2-41291) was used in western blot on rat samples (fig 3f). Oxid Med Cell Longev (2018) ncbi
ProSci
  • western blot; human; loading ...; fig 1e
ProSci CYBB antibody (ProSci, 7923) was used in western blot on human samples (fig 1e). PLoS ONE (2017) ncbi
Articles Reviewed
  1. Rangan P, Lobo F, Parrella E, Rochette N, Morselli M, Stephen T, et al. Fasting-mimicking diet cycles reduce neuroinflammation to attenuate cognitive decline in Alzheimer's models. Cell Rep. 2022;40:111417 pubmed publisher
  2. Wan L, Bai X, Zhou Q, Chen C, Wang H, Liu T, et al. The advanced glycation end-products (AGEs)/ROS/NLRP3 inflammasome axis contributes to delayed diabetic corneal wound healing and nerve regeneration. Int J Biol Sci. 2022;18:809-825 pubmed publisher
  3. Saiyang X, QingQing W, Man X, Chen L, Min Z, Yun X, et al. Activation of Toll-like receptor 7 provides cardioprotection in septic cardiomyopathy-induced systolic dysfunction. Clin Transl Med. 2021;11:e266 pubmed publisher
  4. Wanschel A, Guizoni D, Lorza Gil E, Salerno A, Paiva A, Dorighello G, et al. The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction. Biomolecules. 2021;11: pubmed publisher
  5. Hu D, Dong R, Zhang Y, Yang Y, Chen Z, Tang Y, et al. Age‑related changes in mineralocorticoid receptors in rat hearts. Mol Med Rep. 2020;22:1859-1867 pubmed publisher
  6. Kim Y, Kim J, Gu S, Jo S, Kim S, Young Kim S, et al. Identification of highly potent and selective inhibitor, TIPTP, of the p22phox-Rubicon axis as a therapeutic agent for rheumatoid arthritis. Sci Rep. 2020;10:4570 pubmed publisher
  7. Bitar M, Nader J, Al Ali W, Al Madhoun A, Arefanian H, Al Mulla F. Hydrogen Sulfide Donor NaHS Improves Metabolism and Reduces Muscle Atrophy in Type 2 Diabetes: Implication for Understanding Sarcopenic Pathophysiology. Oxid Med Cell Longev. 2018;2018:6825452 pubmed publisher
  8. Wu Z, Zhao X, Banaszak L, Gutierrez Rodrigues F, Keyvanfar K, Gao S, et al. CRISPR/Cas9-mediated ASXL1 mutations in U937 cells disrupt myeloid differentiation. Int J Oncol. 2018;52:1209-1223 pubmed publisher
  9. Zhai L, Liu M, Wang T, Zhang H, Li S, Guo Y. Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia-reperfusion injury. PLoS ONE. 2017;12:e0174414 pubmed publisher
  10. Thomas D, Clare S, Sowerby J, Pardo M, Juss J, Goulding D, et al. Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity. J Exp Med. 2017;214:1111-1128 pubmed publisher
  11. Pan L, Ye X, Ding J, Zhou Y. Antiproliferation effect of the uremic toxin para?cresol on endothelial progenitor cells is related to its antioxidant activity. Mol Med Rep. 2017;15:2308-2312 pubmed publisher
  12. Jafari N, Kim H, Park R, Li L, Jang M, Morris A, et al. CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasion in HeLa Cells. PLoS ONE. 2017;12:e0170327 pubmed publisher
  13. Wang Y, Chang S, Wu Q, Gou Y, Jia L, Cui Y, et al. The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis. Front Aging Neurosci. 2016;8:308 pubmed publisher
  14. Dolunay A, Senol S, Temiz Resitoglu M, Guden D, Sari A, Sahan Firat S, et al. Inhibition of NLRP3 Inflammasome Prevents LPS-Induced Inflammatory Hyperalgesia in Mice: Contribution of NF-?B, Caspase-1/11, ASC, NOX, and NOS Isoforms. Inflammation. 2017;40:366-386 pubmed publisher
  15. Gu X, Liu X, Chen Y, Zhao Y, Xu M, Han X, et al. Involvement of NADPH oxidases in alkali burn-induced corneal injury. Int J Mol Med. 2016;38:75-82 pubmed publisher
  16. Gharib M, Tao H, Fungwe T, Hajri T. Cluster Differentiating 36 (CD36) Deficiency Attenuates Obesity-Associated Oxidative Stress in the Heart. PLoS ONE. 2016;11:e0155611 pubmed publisher
  17. Zhao Y, Xu L, Qiao Z, Gao L, Ding S, Ying X, et al. YiXin-Shu, a ShengMai-San-based traditional Chinese medicine formula, attenuates myocardial ischemia/reperfusion injury by suppressing mitochondrial mediated apoptosis and upregulating liver-X-receptor α. Sci Rep. 2016;6:23025 pubmed publisher
  18. Dvash E, Har Tal M, Barak S, Meir O, Rubinstein M. Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage. Nat Commun. 2015;6:10112 pubmed publisher
  19. Ellison M, Thurman G, Gearheart C, Seewald R, Porter C, Ambruso D. INF-γ Enhances Nox2 Activity by Upregulating phox Proteins When Applied to Differentiating PLB-985 Cells but Does Not Induce Nox2 Activity by Itself. PLoS ONE. 2015;10:e0136766 pubmed publisher
  20. Graham D, Becker C, Doan A, Goel G, Villablanca E, Knights D, et al. Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst. Nat Commun. 2015;6:7838 pubmed publisher
  21. Lee J, Kim H, Han J, Kim Y, Son C. Anti-fatigue effect of Myelophil in a chronic forced exercise mouse model. Eur J Pharmacol. 2015;764:100-8 pubmed publisher
  22. Kim T, Lawson M. GnRH Regulates Gonadotropin Gene Expression Through NADPH/Dual Oxidase-Derived Reactive Oxygen Species. Endocrinology. 2015;156:2185-99 pubmed publisher
  23. Heiserman J, Chen L, Kim B, Kim S, Tran A, Siebenborn N, et al. TLR4 mutation and HSP60-induced cell death in adult mouse cardiac myocytes. Cell Stress Chaperones. 2015;20:527-35 pubmed publisher
  24. Ajayi A, Yu X, Wahlo Svedin C, Tsirigotaki G, Karlström V, Ström A. Altered p53 and NOX1 activity cause bioenergetic defects in a SCA7 polyglutamine disease model. Biochim Biophys Acta. 2015;1847:418-428 pubmed publisher
  25. Beaumel S, Grunwald D, Fieschi F, Stasia M. Identification of NOX2 regions for normal biosynthesis of cytochrome b558 in phagocytes highlighting essential residues for p22phox binding. Biochem J. 2014;464:425-37 pubmed publisher
  26. Ishaq M, Evans M, Ostrikov K. Atmospheric pressure gas plasma-induced colorectal cancer cell death is mediated by Nox2-ASK1 apoptosis pathways and oxidative stress is mitigated by Srx-Nrf2 anti-oxidant system. Biochim Biophys Acta. 2014;1843:2827-37 pubmed publisher
  27. Jadhav V, Krause K, Singh S. HIV-1 Tat C modulates NOX2 and NOX4 expressions through miR-17 in a human microglial cell line. J Neurochem. 2014;131:803-15 pubmed publisher
  28. Kovacs I, Horvath M, Kovacs T, Somogyi K, Tretter L, Geiszt M, et al. Comparison of proton channel, phagocyte oxidase, and respiratory burst levels between human eosinophil and neutrophil granulocytes. Free Radic Res. 2014;48:1190-9 pubmed publisher