This is a Validated Antibody Database (VAD) review about human CXCL12, based on 23 published articles (read how Labome selects the articles), using CXCL12 antibody in all methods. It is aimed to help Labome visitors find the most suited CXCL12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CXCL12 synonym: IRH; PBSF; SCYB12; SDF1; TLSF; TPAR1; stromal cell-derived factor 1; chemokine (C-X-C motif) ligand 12; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor

R&D Systems
mouse monoclonal (79014)
  • immunohistochemistry; human; 1:600; loading ...; fig 6
R&D Systems CXCL12 antibody (R&D Systems, MAB310) was used in immunohistochemistry on human samples at 1:600 (fig 6). J Histochem Cytochem (2018) ncbi
mouse monoclonal (79018)
  • flow cytometry; mouse; 1:10; loading ...; fig 2h
R&D Systems CXCL12 antibody (R&D Systems, IC350A) was used in flow cytometry on mouse samples at 1:10 (fig 2h). Nat Cell Biol (2017) ncbi
mouse monoclonal (79018)
  • immunohistochemistry - paraffin section; rat; 1:40; loading ...; fig st14
In order to outline the protocols for antibodies used for immunohistochemical studies, R&D Systems CXCL12 antibody (R&D System??, MAB350) was used in immunohistochemistry - paraffin section on rat samples at 1:40 (fig st14). J Toxicol Pathol (2017) ncbi
mouse monoclonal (79018)
  • immunohistochemistry - frozen section; human; loading ...; fig 4
R&D Systems CXCL12 antibody (R&D Systems, 79018) was used in immunohistochemistry - frozen section on human samples (fig 4). J Dermatol Sci (2017) ncbi
mouse monoclonal (79014)
  • blocking or activating experiments; mouse; fig 4a
R&D Systems CXCL12 antibody (R&D Systems, 79014) was used in blocking or activating experiments on mouse samples (fig 4a). Cancer Res (2016) ncbi
goat polyclonal
  • immunohistochemistry; human; 1:100; fig 1
R&D Systems CXCL12 antibody (R&D Systems , AF-310-NA) was used in immunohistochemistry on human samples at 1:100 (fig 1). Int J Cancer (2016) ncbi
goat polyclonal
  • immunohistochemistry; human; 1:1000
  • immunohistochemistry; mouse; 1:1000
In order to show that biliary epithelial cells are not the primary source of CXLC12, R&D Systems CXCL12 antibody (R&D Systems, AF-310-NA) was used in immunohistochemistry on human samples at 1:1000 and in immunohistochemistry on mouse samples at 1:1000. Am J Pathol (2015) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:200; loading ...; fig 5d
Abcam CXCL12 antibody (Abcam, ab9797) was used in immunohistochemistry - frozen section on human samples at 1:200 (fig 5d). J Histochem Cytochem (2018) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:200; fig 5b
In order to suggest that double homeobox transcription factor 4 regulates migration of mesenchymal stem cells via the C-X-C motif Receptor 4 receptor, Abcam CXCL12 antibody (Abcam, ab 9797) was used in immunohistochemistry on human samples at 1:200 (fig 5b). Oncotarget (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 3
Abcam CXCL12 antibody (Abcam, ab9797) was used in western blot on mouse samples (fig 3). Oncogene (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:500
Abcam CXCL12 antibody (Abcam, ab9797) was used in immunohistochemistry - paraffin section on human samples at 1:500. Hum Pathol (2014) ncbi
rabbit polyclonal
  • ELISA; human; 1:500
Abcam CXCL12 antibody (Abcam, ab9797) was used in ELISA on human samples at 1:500. Cell Tissue Res (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200
Abcam CXCL12 antibody (Abcam, ab9797) was used in immunohistochemistry - paraffin section on human samples at 1:200. PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; mouse
Abcam CXCL12 antibody (Abcam Inc., ab9797) was used in western blot on mouse samples . Am J Pathol (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000
Abcam CXCL12 antibody (Abcam, ab9797) was used in western blot on rat samples at 1:1000. Diabetes Res Clin Pract (2013) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; loading ...; fig 1d
In order to assess the male genital compartment in patients with chronic prostatitis/chronic pelvic pain syndrome, Invitrogen CXCL12 antibody (Thermo Fisher Scientific, PA5-30603) was used in western blot on human samples (fig 1d). Urol Int (2017) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000
In order to investigate the mechanisms by which mesenchymal stem cells alleviate acute liver failure in rats, Invitrogen CXCL12 antibody (Thermo Scientific, PA5-17238) was used in western blot on rat samples at 1:1000. Genet Mol Res (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; fig 6
Cell Signaling Technology CXCL12 antibody (Cell Signaling, 3740) was used in western blot on human samples (fig 6). Oncotarget (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
Cell Signaling Technology CXCL12 antibody (Cell Signaling Technology, 3740S) was used in western blot on mouse samples at 1:1000. Exp Ther Med (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000
Cell Signaling Technology CXCL12 antibody (Cell Signaling Technology, 3740S) was used in western blot on rat samples at 1:1000. Mol Med Rep (2015) ncbi
rabbit monoclonal (D32F9)
  • western blot; human; 1:1000
Cell Signaling Technology CXCL12 antibody (Cell Signaling Technology, 3530) was used in western blot on human samples at 1:1000. Mol Cell Biochem (2015) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology CXCL12 antibody (Cell Signaling, 3740) was used in western blot on human samples . PLoS ONE (2013) ncbi
EMD Millipore
mouse monoclonal (K15C)
  • immunohistochemistry - frozen section; human; fig 4
EMD Millipore CXCL12 antibody (Millipore, K15C) was used in immunohistochemistry - frozen section on human samples (fig 4). PLoS ONE (2015) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunoprecipitation; human; 10 ug/ml
  • western blot; human
Sigma-Aldrich CXCL12 antibody (Sigma-Aldrich, AV07031) was used in immunoprecipitation on human samples at 10 ug/ml and in western blot on human samples . PLoS ONE (2015) ncbi
Articles Reviewed
  1. Hira V, Wormer J, Kakar H, Breznik B, van der Swaan B, Hulsbos R, et al. Periarteriolar Glioblastoma Stem Cell Niches Express Bone Marrow Hematopoietic Stem Cell Niche Proteins. J Histochem Cytochem. 2018;66:155-173 pubmed publisher
  2. Asada N, Kunisaki Y, Pierce H, Wang Z, Fernandez N, Birbrair A, et al. Differential cytokine contributions of perivascular haematopoietic stem cell niches. Nat Cell Biol. 2017;19:214-223 pubmed publisher
  3. Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  4. Ujiie I, Fujita Y, Nakayama C, Matsumura W, Suzuki S, Shinkuma S, et al. Altered balance of epidermis-related chemokines in epidermolysis bullosa. J Dermatol Sci. 2017;86:37-45 pubmed publisher
  5. Schagdarsurengin U, Teuchert L, Hagenkötter C, Nesheim N, Dansranjavin T, Schuppe H, et al. Chronic Prostatitis Affects Male Reproductive Health and Is Associated with Systemic and Local Epigenetic Inactivation of C-X-C Motif Chemokine 12 Receptor C-X-C Chemokine Receptor Type 4. Urol Int. 2017;98:89-101 pubmed publisher
  6. Dmitriev P, Kiseleva E, Kharchenko O, Ivashkin E, Pichugin A, Dessen P, et al. Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis. Oncotarget. 2016;7:65090-65108 pubmed publisher
  7. Martinelli G, Olivari D, Re Cecconi A, Talamini L, Ottoboni L, Lecker S, et al. Activation of the SDF1/CXCR4 pathway retards muscle atrophy during cancer cachexia. Oncogene. 2016;35:6212-6222 pubmed publisher
  8. Ananthula S, Sinha A, El Gassim M, Batth S, Marshall G, Gardner L, et al. Geminin overexpression-dependent recruitment and crosstalk with mesenchymal stem cells enhance aggressiveness in triple negative breast cancers. Oncotarget. 2016;7:20869-89 pubmed publisher
  9. Kasagi Y, Harada Y, Morodomi Y, Iwai T, Saito S, Yoshida K, et al. Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix-Directed Spheroid Formation. Cancer Res. 2016;76:347-57 pubmed publisher
  10. Izumi D, Ishimoto T, Miyake K, Sugihara H, Eto K, Sawayama H, et al. CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin β1 clustering and invasiveness in gastric cancer. Int J Cancer. 2016;138:1207-19 pubmed publisher
  11. Jing L, Li S, Li Q. Akt/hypoxia-inducible factor-1α signaling deficiency compromises skin wound healing in a type 1 diabetes mouse model. Exp Ther Med. 2015;9:2141-2146 pubmed
  12. Wang Y, Li Q, Wei X, Xu J, Chen Q, Song S, et al. Targeted knockout of TNF-? by injection of lentivirus-mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats. Mol Med Rep. 2015;12:4389-4395 pubmed publisher
  13. Nobutani K, Shimono Y, Mizutani K, Ueda Y, Suzuki T, Kitayama M, et al. Downregulation of CXCR4 in Metastasized Breast Cancer Cells and Implication in Their Dormancy. PLoS ONE. 2015;10:e0130032 pubmed publisher
  14. Saiman Y, Sugiyama T, Simchoni N, Spirli C, Bansal M. Biliary Epithelial Cells Are Not the Predominant Source of Hepatic CXCL12. Am J Pathol. 2015;185:1859-66 pubmed publisher
  15. Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, et al. Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines. PLoS ONE. 2015;10:e0120069 pubmed publisher
  16. Zhang J, Zhao J, Bai Y, Huang L, Yu W, Li X. Effects of p75 neurotrophin receptor on regulating hypoxia-induced angiogenic factors in retinal pigment epithelial cells. Mol Cell Biochem. 2015;398:123-34 pubmed publisher
  17. Mitchell B, Leone D, Feller K, Menon S, Bondzie P, Yang S, et al. Protein expression of the chemokine receptor CXCR4 and its ligand CXCL12 in primary cutaneous melanoma--biomarkers of potential utility?. Hum Pathol. 2014;45:2094-100 pubmed publisher
  18. Yuan S, Jiang T, Sun L, Zheng R, Cao G, Ahat N, et al. Use of bone mesenchymal stem cells to treat rats with acute liver failure. Genet Mol Res. 2014;13:6962-80 pubmed publisher
  19. Hattermann K, Holzenburg E, Hans F, Lucius R, Held Feindt J, Mentlein R. Effects of the chemokine CXCL12 and combined internalization of its receptors CXCR4 and CXCR7 in human MCF-7 breast cancer cells. Cell Tissue Res. 2014;357:253-66 pubmed publisher
  20. Walentowicz Sadlecka M, Sadlecki P, Bodnar M, Marszalek A, Walentowicz P, Sokup A, et al. Stromal derived factor-1 (SDF-1) and its receptors CXCR4 and CXCR7 in endometrial cancer patients. PLoS ONE. 2014;9:e84629 pubmed publisher
  21. Li J, Tan H, Wang X, Li Y, Samuelson L, Li X, et al. Circulating fibrocytes stabilize blood vessels during angiogenesis in a paracrine manner. Am J Pathol. 2014;184:556-71 pubmed publisher
  22. Florea V, Bhagavatula N, Simovic G, Macedo F, Fock R, Rodrigues C. c-Myc is essential to prevent endothelial pro-inflammatory senescent phenotype. PLoS ONE. 2013;8:e73146 pubmed publisher
  23. Hou Z, Nie C, Si Z, Ma Y. Deferoxamine enhances neovascularization and accelerates wound healing in diabetic rats via the accumulation of hypoxia-inducible factor-1?. Diabetes Res Clin Pract. 2013;101:62-71 pubmed publisher