This is a Validated Antibody Database (VAD) review about human CTCF, based on 29 published articles (read how Labome selects the articles), using CTCF antibody in all methods. It is aimed to help Labome visitors find the most suited CTCF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CTCF synonym: MRD21

Abcam
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s5a
Abcam CTCF antibody (Abcam, ab70303) was used in western blot on mouse samples at 1:1000 (fig s5a). Cell Rep (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1c
Abcam CTCF antibody (abcam, ab70303) was used in western blot on human samples at 1:1000 (fig 1c). Nucleic Acids Res (2019) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 6a
Abcam CTCF antibody (Abcam, ab70303) was used in immunoprecipitation on human samples (fig 6a). Proc Natl Acad Sci U S A (2018) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 1
In order to investigate the function of CTCF sites in the casein locus, Abcam CTCF antibody (Abcam, ab70303) was used in ChIP-Seq on mouse samples (fig 1). Nucleic Acids Res (2017) ncbi
mouse monoclonal (mAbcam 37477)
  • western blot; human; fig 3
In order to study how SNF2H organizes nucleosomes, Abcam CTCF antibody (Abcam, ab37477) was used in western blot on human samples (fig 3). PLoS Genet (2016) ncbi
domestic rabbit monoclonal (EPR7314(B))
  • western blot; mouse; 1:500; fig 4
Abcam CTCF antibody (Abcam, ab128873) was used in western blot on mouse samples at 1:500 (fig 4). J Cell Biol (2015) ncbi
Active Motif
domestic rabbit polyclonal
  • ChIP-Seq; human; loading ...; fig 5b
Active Motif CTCF antibody (Active Motif, 61311) was used in ChIP-Seq on human samples (fig 5b). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; human; fig s11a
Active Motif CTCF antibody (Active Motif, 61311) was used in ChIP-Seq on human samples (fig s11a). Nat Genet (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 1c
  • immunoprecipitation; mouse; loading ...; fig 1b
  • western blot; mouse; loading ...; fig 1b
Cell Signaling Technology CTCF antibody (Cell Signaling, 2899) was used in ChIP-Seq on mouse samples (fig 1c), in immunoprecipitation on mouse samples (fig 1b) and in western blot on mouse samples (fig 1b). Cell (2019) ncbi
domestic rabbit monoclonal (D31H2)
  • ChIP-Seq; mouse; loading ...; fig 4c
  • immunocytochemistry; mouse; loading ...; fig 3c
  • western blot; mouse; loading ...; fig 1c
  • immunocytochemistry; human; loading ...; fig 2e
Cell Signaling Technology CTCF antibody (Cell Signaling, 3418) was used in ChIP-Seq on mouse samples (fig 4c), in immunocytochemistry on mouse samples (fig 3c), in western blot on mouse samples (fig 1c) and in immunocytochemistry on human samples (fig 2e). elife (2019) ncbi
domestic rabbit monoclonal (D31H2)
  • western blot; chicken; loading ...; fig s14a
Cell Signaling Technology CTCF antibody (Cell Signaling, 3418S) was used in western blot on chicken samples (fig s14a). Nucleic Acids Res (2019) ncbi
domestic rabbit monoclonal (D31H2)
  • western blot; human; 1:700; loading ...; fig s8a
In order to identify the elementary structural units of the DNA damage response, Cell Signaling Technology CTCF antibody (Cell Signaling, D31H2) was used in western blot on human samples at 1:700 (fig s8a). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D31H2)
  • RNA immunoprecipitation; human; fig 3
Cell Signaling Technology CTCF antibody (Cell Signaling, 3418S) was used in RNA immunoprecipitation on human samples (fig 3). Genome Biol (2016) ncbi
domestic rabbit monoclonal (D31H2)
  • western blot; human; 1:1000; fig 3
Cell Signaling Technology CTCF antibody (Cell Signaling Technology, 3418) was used in western blot on human samples at 1:1000 (fig 3). PLoS ONE (2016) ncbi
domestic rabbit monoclonal (D31H2)
  • chromatin immunoprecipitation; human; fig 3
Cell Signaling Technology CTCF antibody (Cell Signaling, 3418) was used in chromatin immunoprecipitation on human samples (fig 3). Nature (2016) ncbi
EMD Millipore
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig e1g
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on mouse samples (fig e1g). Nature (2019) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 5a
EMD Millipore CTCF antibody (Millipore Sigma, 07-729) was used in ChIP-Seq on mouse samples (fig 5a). Nucleic Acids Res (2019) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig e6j
EMD Millipore CTCF antibody (Millipore, 07-729) was used in ChIP-Seq on mouse samples (fig e6j). Nature (2019) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 2f
EMD Millipore CTCF antibody (Millipore, 07-729) was used in chromatin immunoprecipitation on human samples (fig 2f). Nat Genet (2017) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 1c
EMD Millipore CTCF antibody (Upstate, 07-729) was used in ChIP-Seq on mouse samples (fig 1c). Nature (2017) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 1
In order to investigate the function of CTCF sites in the casein locus, EMD Millipore CTCF antibody (Millipore, 07-729) was used in ChIP-Seq on mouse samples (fig 1). Nucleic Acids Res (2017) ncbi
domestic rabbit polyclonal
  • EMSA; human; loading ...; fig 5d
EMD Millipore CTCF antibody (Upstate-Millipore, 07-729) was used in EMSA on human samples (fig 5d). Epigenetics (2017) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 5a
EMD Millipore CTCF antibody (Millipore, 07-729) was used in ChIP-Seq on mouse samples (fig 5a). Biochim Biophys Acta Gene Regul Mech (2017) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig s7a
  • ChIP-Seq; mouse; loading ...; fig 7a
In order to characterize Aire-mediated signaling in medullary thymic epithelial cells, EMD Millipore CTCF antibody (Upstate, 07-729) was used in chromatin immunoprecipitation on human samples (fig s7a) and in ChIP-Seq on mouse samples (fig 7a). Nat Immunol (2017) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; fig 2
EMD Millipore CTCF antibody (millipore, 07-729) was used in ChIP-Seq on mouse samples (fig 2). Genome Biol (2016) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; human; loading ...; fig 2a
EMD Millipore CTCF antibody (Millipore, 07-729) was used in ChIP-Seq on human samples (fig 2a). EMBO Rep (2016) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 5
  • western blot; human; fig 5h
EMD Millipore CTCF antibody (EMD Millipore, 07-729) was used in chromatin immunoprecipitation on human samples (fig 5) and in western blot on human samples (fig 5h). PLoS Pathog (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1a,b
EMD Millipore CTCF antibody (Millipore, 07-729) was used in western blot on human samples (fig 1a,b). Nucleic Acids Res (2016) ncbi
BD Biosciences
mouse monoclonal (48/CTCF)
  • immunocytochemistry; human; 1:50; fig 7
  • western blot; human; fig 9
BD Biosciences CTCF antibody (BD, 612149) was used in immunocytochemistry on human samples at 1:50 (fig 7) and in western blot on human samples (fig 9). PLoS Pathog (2015) ncbi
mouse monoclonal (48/CTCF)
  • immunocytochemistry; human
BD Biosciences CTCF antibody (BD, 612149) was used in immunocytochemistry on human samples . PLoS Genet (2014) ncbi
Articles Reviewed
  1. Rhodes J, Feldmann A, Hernández Rodríguez B, Díaz N, Brown J, Fursova N, et al. Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells. Cell Rep. 2020;30:820-835.e10 pubmed publisher
  2. Zhang H, Emerson D, Gilgenast T, Titus K, Lan Y, Huang P, et al. Chromatin structure dynamics during the mitosis-to-G1 phase transition. Nature. 2019;576:158-162 pubmed publisher
  3. Kaaij L, Mohn F, van der Weide R, de Wit E, B hler M. The ChAHP Complex Counteracts Chromatin Looping at CTCF Sites that Emerged from SINE Expansions in Mouse. Cell. 2019;178:1437-1451.e14 pubmed publisher
  4. Hyle J, Zhang Y, Wright S, Xu B, Shao Y, Easton J, et al. Acute depletion of CTCF directly affects MYC regulation through loss of enhancer-promoter looping. Nucleic Acids Res. 2019;: pubmed publisher
  5. Zhang S, Deng T, Tang W, He B, Furusawa T, Ambs S, et al. Epigenetic regulation of REX1 expression and chromatin binding specificity by HMGNs. Nucleic Acids Res. 2019;47:4449-4461 pubmed publisher
  6. Del Rosario B, Kriz A, Del Rosario A, Anselmo A, Fry C, White F, et al. Exploration of CTCF post-translation modifications uncovers Serine-224 phosphorylation by PLK1 at pericentric regions during the G2/M transition. elife. 2019;8: pubmed publisher
  7. MONAHAN K, HORTA A, Lomvardas S. LHX2- and LDB1-mediated trans interactions regulate olfactory receptor choice. Nature. 2019;565:448-453 pubmed publisher
  8. Fishman V, Battulin N, Nuriddinov M, Maslova A, Zlotina A, Strunov A, et al. 3D organization of chicken genome demonstrates evolutionary conservation of topologically associated domains and highlights unique architecture of erythrocytes' chromatin. Nucleic Acids Res. 2019;47:648-665 pubmed publisher
  9. Stewart E, McEvoy J, Wang H, Chen X, Honnell V, Ocarz M, et al. Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses. Cancer Cell. 2018;34:411-426.e19 pubmed publisher
  10. Castanotto D, Zhang X, Alluin J, Zhang X, Rüger J, Armstrong B, et al. A stress-induced response complex (SIRC) shuttles miRNAs, siRNAs, and oligonucleotides to the nucleus. Proc Natl Acad Sci U S A. 2018;115:E5756-E5765 pubmed publisher
  11. Rubin A, Barajas B, Furlan Magaril M, Lopez Pajares V, Mumbach M, Howard I, et al. Lineage-specific dynamic and pre-established enhancer-promoter contacts cooperate in terminal differentiation. Nat Genet. 2017;49:1522-1528 pubmed publisher
  12. Natale F, Rapp A, Yu W, Maiser A, Harz H, Scholl A, et al. Identification of the elementary structural units of the DNA damage response. Nat Commun. 2017;8:15760 pubmed publisher
  13. Busslinger G, Stocsits R, van der Lelij P, Axelsson E, Tedeschi A, Galjart N, et al. Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl. Nature. 2017;544:503-507 pubmed publisher
  14. Lee H, Willi M, Wang C, Yang C, Smith H, Liu C, et al. Functional assessment of CTCF sites at cytokine-sensing mammary enhancers using CRISPR/Cas9 gene editing in mice. Nucleic Acids Res. 2017;45:4606-4618 pubmed publisher
  15. Hu T, Zhu X, Pi W, Yu M, Shi H, Tuan D. Hypermethylated LTR retrotransposon exhibits enhancer activity. Epigenetics. 2017;12:226-237 pubmed publisher
  16. Wu H, Gordon J, Whitfield T, Tai P, Van Wijnen A, Stein J, et al. Chromatin dynamics regulate mesenchymal stem cell lineage specification and differentiation to osteogenesis. Biochim Biophys Acta Gene Regul Mech. 2017;1860:438-449 pubmed publisher
  17. Herzig Y, Nevo S, Bornstein C, Brezis M, Ben Hur S, Shkedy A, et al. Transcriptional programs that control expression of the autoimmune regulator gene Aire. Nat Immunol. 2017;18:161-172 pubmed publisher
  18. Weischenfeldt J, Dubash T, Drainas A, Mardin B, Chen Y, Stütz A, et al. Pan-cancer analysis of somatic copy-number alterations implicates IRS4 and IGF2 in enhancer hijacking. Nat Genet. 2017;49:65-74 pubmed publisher
  19. Uusküla Reimand L, Hou H, Samavarchi Tehrani P, Rudan M, Liang M, Medina Rivera A, et al. Topoisomerase II beta interacts with cohesin and CTCF at topological domain borders. Genome Biol. 2016;17:182 pubmed publisher
  20. Platt J, Salama R, Smythies J, Choudhry H, Davies J, Hughes J, et al. Capture-C reveals preformed chromatin interactions between HIF-binding sites and distant promoters. EMBO Rep. 2016;17:1410-1421 pubmed
  21. Wiechens N, Singh V, Gkikopoulos T, Schofield P, Rocha S, Owen Hughes T. The Chromatin Remodelling Enzymes SNF2H and SNF2L Position Nucleosomes adjacent to CTCF and Other Transcription Factors. PLoS Genet. 2016;12:e1005940 pubmed publisher
  22. G Hendrickson D, Kelley D, Tenen D, BERNSTEIN B, Rinn J. Widespread RNA binding by chromatin-associated proteins. Genome Biol. 2016;17:28 pubmed publisher
  23. Pchelintsev N, Adams P, Nelson D. Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins. PLoS ONE. 2016;11:e0148023 pubmed publisher
  24. Lu F, Chen H, Kossenkov A, DeWispeleare K, Won K, Lieberman P. EBNA2 Drives Formation of New Chromosome Binding Sites and Target Genes for B-Cell Master Regulatory Transcription Factors RBP-jκ and EBF1. PLoS Pathog. 2016;12:e1005339 pubmed publisher
  25. Flavahan W, Drier Y, Liau B, Gillespie S, Venteicher A, Stemmer Rachamimov A, et al. Insulator dysfunction and oncogene activation in IDH mutant gliomas. Nature. 2016;529:110-4 pubmed publisher
  26. Yun J, Song S, Kang J, Park J, Kim H, Han S, et al. Reduced cohesin destabilizes high-level gene amplification by disrupting pre-replication complex bindings in human cancers with chromosomal instability. Nucleic Acids Res. 2016;44:558-72 pubmed publisher
  27. Mehta K, Gunasekharan V, Satsuka A, Laimins L. Human papillomaviruses activate and recruit SMC1 cohesin proteins for the differentiation-dependent life cycle through association with CTCF insulators. PLoS Pathog. 2015;11:e1004763 pubmed publisher
  28. Harr J, Luperchio T, Wong X, Cohen E, Wheelan S, Reddy K. Directed targeting of chromatin to the nuclear lamina is mediated by chromatin state and A-type lamins. J Cell Biol. 2015;208:33-52 pubmed publisher
  29. Zuin J, Franke V, van Ijcken W, van der Sloot A, Krantz I, van der Reijden M, et al. A cohesin-independent role for NIPBL at promoters provides insights in CdLS. PLoS Genet. 2014;10:e1004153 pubmed publisher