This is a Validated Antibody Database (VAD) review about human CPSF6, based on 7 published articles (read how Labome selects the articles), using CPSF6 antibody in all methods. It is aimed to help Labome visitors find the most suited CPSF6 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CPSF6 synonym: CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7; cleavage and polyadenylation specificity factor subunit 6; CPSF 68 kDa subunit; cleavage and polyadenylation specific factor 6, 68kDa; cleavage and polyadenylation specificity factor 68 kDa subunit; cleavage factor Im complex 68 kDa subunit; pre-mRNA cleavage factor I, 68kD subunit; pre-mRNA cleavage factor Im (68kD); pre-mRNA cleavage factor Im 68 kDa subunit; protein HPBRII-4/7

Knockout validation
Bethyl
rabbit polyclonal
  • western blot knockout validation; human; fig 1
Bethyl CPSF6 antibody (Bethyl Laboratories, A301-358A) was used in western blot knockout validation on human samples (fig 1). Proc Natl Acad Sci U S A (2016) ncbi
Abcam
rabbit monoclonal (EPR12898)
  • western blot knockout validation; human; fig 1
Abcam CPSF6 antibody (Abcam, ab175237) was used in western blot knockout validation on human samples (fig 1). Proc Natl Acad Sci U S A (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-3)
  • western blot knockout validation; human; fig 4
Santa Cruz Biotechnology CPSF6 antibody (Santa Cruz Biotechnology, sc376228) was used in western blot knockout validation on human samples (fig 4). J Biol Chem (2016) ncbi
Bethyl
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 3
Bethyl CPSF6 antibody (Bethyl Laboratories, A301-356A) was used in western blot on mouse samples at 1:1000 (fig 3). Cell Rep (2016) ncbi
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl CPSF6 antibody (Bethyl, A301-356A) was used in RNA immunoprecipitation on human samples and in immunocytochemistry on human samples at 2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot knockout validation; human; fig 1
Bethyl CPSF6 antibody (Bethyl Laboratories, A301-358A) was used in western blot knockout validation on human samples (fig 1). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
Bethyl CPSF6 antibody (Bethyl Laboratories, A301-356A) was used in western blot on mouse samples (fig 5). Genes Dev (2015) ncbi
Abcam
rabbit monoclonal (EPR12898)
  • western blot; mouse; loading ...; fig 5g
Abcam CPSF6 antibody (Abcam, ab175237) was used in western blot on mouse samples (fig 5g). Nucleic Acids Res (2018) ncbi
rabbit monoclonal (EPR12898)
  • western blot knockout validation; human; fig 1
Abcam CPSF6 antibody (Abcam, ab175237) was used in western blot knockout validation on human samples (fig 1). Proc Natl Acad Sci U S A (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-3)
  • western blot knockout validation; human; fig 4
Santa Cruz Biotechnology CPSF6 antibody (Santa Cruz Biotechnology, sc376228) was used in western blot knockout validation on human samples (fig 4). J Biol Chem (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; fig 6
In order to study multimodal inhibition of HIV-1 by PF74 and the roles of capsid-interacting host factors, Proteintech Group CPSF6 antibody (Proteintech, 15489-1-AP) was used in western blot on human samples (fig 6). J Virol (2016) ncbi
Articles Reviewed
  1. Chang J, Zhang W, Yeh H, Park M, Yao C, Shi Y, et al. An integrative model for alternative polyadenylation, IntMAP, delineates mTOR-modulated endoplasmic reticulum stress response. Nucleic Acids Res. 2018;46:5996-6008 pubmed publisher
  2. Saito A, Ferhadian D, Sowd G, Serrao E, Shi J, Halambage U, et al. Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74. J Virol. 2016;90:5808-5823 pubmed publisher
  3. Hwang H, Park C, Goodarzi H, Fak J, Mele A, Moore M, et al. PAPERCLIP Identifies MicroRNA Targets and a Role of CstF64/64tau in Promoting Non-canonical poly(A) Site Usage. Cell Rep. 2016;15:423-35 pubmed publisher
  4. Rasheedi S, Shun M, Serrao E, Sowd G, Qian J, Hao C, et al. The Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Subunit of the Capsid-recruited Pre-messenger RNA Cleavage Factor I (CFIm) Complex Mediates HIV-1 Integration into Genes. J Biol Chem. 2016;291:11809-19 pubmed publisher
  5. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  6. Sowd G, Serrao E, Wang H, Wang W, Fadel H, Poeschla E, et al. A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin. Proc Natl Acad Sci U S A. 2016;113:E1054-63 pubmed publisher
  7. Masuda A, Takeda J, Okuno T, Okamoto T, Ohkawara B, Ito M, et al. Position-specific binding of FUS to nascent RNA regulates mRNA length. Genes Dev. 2015;29:1045-57 pubmed publisher