This is a Validated Antibody Database (VAD) review about human COX4I2, based on 9 published articles (read how Labome selects the articles), using COX4I2 antibody in all methods. It is aimed to help Labome visitors find the most suited COX4I2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
COX4I2 synonym: COX4; COX4-2; COX4B; COX4L2; COXIV-2; dJ857M17.2; cytochrome c oxidase subunit 4 isoform 2, mitochondrial; COX IV-2; cytochrome c oxidase subunit IV isoform 2 (lung); cytochrome c oxidase subunit IV-like 2

Abcam
mouse monoclonal (10G8D12C12)
  • western blot; human; fig 1a
Abcam COX4I2 antibody (Abcam, ab110261) was used in western blot on human samples (fig 1a). Hum Mol Genet (2018) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 4h
Abcam COX4I2 antibody (Abcam, Ab70112) was used in western blot on human samples (fig 4h). EMBO Rep (2017) ncbi
mouse monoclonal (10G8D12C12)
  • western blot; human; loading ...
In order to investigate the role of Complex I accessory subunits, Abcam COX4I2 antibody (Abcam, ab110261) was used in western blot on human samples . Nature (2016) ncbi
mouse monoclonal (10G8D12C12)
  • immunohistochemistry; human; 1:100; fig 5
In order to use CLARITY to study mechanisms of neurodegeneration that occur in mitochondrial disease, Abcam COX4I2 antibody (Abcam, ab110261) was used in immunohistochemistry on human samples at 1:100 (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (10G8D12C12)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig st2
In order to measure mitochondrial respiratory chain protein expression in Purkinje cell bodies and their synaptic terminals in the dentate nucleus, Abcam COX4I2 antibody (Abcam, ab110261) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig st2). Neuropathol Appl Neurobiol (2016) ncbi
mouse monoclonal (10G8D12C12)
  • western blot; human
Abcam COX4I2 antibody (Abcam, 10G8D12C12) was used in western blot on human samples . Mol Immunol (2015) ncbi
mouse monoclonal (10G8D12C12)
  • western blot; mouse
  • western blot; rat
  • western blot; human
Abcam COX4I2 antibody (Abcam, ab110261) was used in western blot on mouse samples , in western blot on rat samples and in western blot on human samples . PLoS ONE (2013) ncbi
Abnova
mouse monoclonal (1F2)
  • western blot; mouse; 1:1000; fig s9
Abnova COX4I2 antibody (Abnova, H00084701-M01) was used in western blot on mouse samples at 1:1000 (fig s9). Nat Med (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; 1:1000; fig 2
In order to characterize BMI1 expression and proliferative capacity of high-grade gliomas regulated by nuclear-encoded cytochrome c oxcidse subunit 4, Proteintech Group COX4I2 antibody (ProteinTech, 11463-1-AP) was used in western blot on human samples at 1:1000 (fig 2). Oncotarget (2015) ncbi
Articles Reviewed
  1. Straub I, Janer A, Weraarpachai W, Zinman L, Robertson J, Rogaeva E, et al. Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS. Hum Mol Genet. 2018;27:178-189 pubmed publisher
  2. Bourens M, Barrientos A. A CMC1-knockout reveals translation-independent control of human mitochondrial complex IV biogenesis. EMBO Rep. 2017;18:477-494 pubmed publisher
  3. Stroud D, Surgenor E, Formosa L, Reljic B, Frazier A, Dibley M, et al. Accessory subunits are integral for assembly and function of human mitochondrial complex I. Nature. 2016;538:123-126 pubmed publisher
  4. Phillips J, Laude A, Lightowlers R, Morris C, Turnbull D, Lax N. Development of passive CLARITY and immunofluorescent labelling of multiple proteins in human cerebellum: understanding mechanisms of neurodegeneration in mitochondrial disease. Sci Rep. 2016;6:26013 pubmed publisher
  5. Cloonan S, Glass K, Laucho Contreras M, Bhashyam A, Cervo M, Pabón M, et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med. 2016;22:163-74 pubmed publisher
  6. Chrysostomou A, Grady J, Laude A, Taylor R, Turnbull D, Lax N. Investigating complex I deficiency in Purkinje cells and synapses in patients with mitochondrial disease. Neuropathol Appl Neurobiol. 2016;42:477-92 pubmed publisher
  7. Ebsen H, Lettau M, Kabelitz D, Janssen O. Subcellular localization and activation of ADAM proteases in the context of FasL shedding in T lymphocytes. Mol Immunol. 2015;65:416-28 pubmed publisher
  8. Oliva C, Markert T, Gillespie G, Griguer C. Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas. Oncotarget. 2015;6:4330-44 pubmed
  9. Kovarova N, Mracek T, Nůsková H, Holzerová E, Vrbacky M, Pecina P, et al. High molecular weight forms of mammalian respiratory chain complex II. PLoS ONE. 2013;8:e71869 pubmed publisher