This is a Validated Antibody Database (VAD) review about human CHRM2, based on 18 published articles (read how Labome selects the articles), using CHRM2 antibody in all methods. It is aimed to help Labome visitors find the most suited CHRM2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CHRM2 synonym: HM2; muscarinic acetylcholine receptor M2; 7TM receptor; acetylcholine receptor, muscarinic 2; cholinergic receptor, muscarinic 2; muscarinic M2 receptor

Abcam
rabbit monoclonal (EPR4568)
  • western blot; mouse; 1:1000; fig 7b
Abcam CHRM2 antibody (Abcam, ab109226) was used in western blot on mouse samples at 1:1000 (fig 7b). elife (2017) ncbi
mouse monoclonal (31-1D1)
  • western blot; rat; 1:2000; fig 2
Abcam CHRM2 antibody (Abcam, ab2805) was used in western blot on rat samples at 1:2000 (fig 2). Behav Brain Funct (2016) ncbi
rabbit monoclonal (EPR4568)
  • immunohistochemistry; rat; 1:200; loading ...; fig 6e
Abcam CHRM2 antibody (Abcam, ab109226) was used in immunohistochemistry on rat samples at 1:200 (fig 6e). Front Cell Neurosci (2016) ncbi
mouse monoclonal (31-1D1)
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:500
Abcam CHRM2 antibody (Abcam, ab2805) was used in immunocytochemistry on human samples at 1:200 and in western blot on human samples at 1:500. J Cell Mol Med (2013) ncbi
Alomone Labs
rabbit polyclonal
  • immunohistochemistry - frozen section; rat; 1:200; fig 1
Alomone Labs CHRM2 antibody (Alomone, AMR-002) was used in immunohistochemistry - frozen section on rat samples at 1:200 (fig 1). Front Pharmacol (2016) ncbi
rabbit polyclonal
  • western blot; rat; fig 8
In order to assess the impact of central dopaminergic denervation on distal colonic excitatory cholinergic neuromotor activity in rats, Alomone Labs CHRM2 antibody (Alomone, MR002) was used in western blot on rat samples (fig 8). J Pharmacol Exp Ther (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (31-3B7)
  • western blot; rat; 1:200; loading ...; fig 3
In order to correlate detrusor function and voiding patterns with muscarinic receptors expression, nerve fiber density, and neural ultrastructure in patients with chronic bladder ischemia, Santa Cruz Biotechnology CHRM2 antibody (Santa Cruz, sc-80971) was used in western blot on rat samples at 1:200 (fig 3). Investig Clin Urol (2016) ncbi
LifeSpan Biosciences
mouse monoclonal (B8E5)
  • western blot; Domestic guinea pig
  • western blot; mouse
  • western blot; human
LifeSpan Biosciences CHRM2 antibody (LifeSpan BioScience, LS-C124189) was used in western blot on Domestic guinea pig samples , in western blot on mouse samples and in western blot on human samples . Am J Respir Cell Mol Biol (2014) ncbi
MilliporeSigma
rabbit polyclonal
  • immunohistochemistry; rat; 1:200; loading ...
In order to determine if medial or lateral habenula or their subnuclei display field-characteristic gene products, MilliporeSigma CHRM2 antibody (Sigma-Aldrich, M9558) was used in immunohistochemistry on rat samples at 1:200. Brain Struct Funct (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:300; fig 2e
MilliporeSigma CHRM2 antibody (Sigma Aldrich, M9558) was used in immunohistochemistry on mouse samples at 1:300 (fig 2e). Am J Respir Cell Mol Biol (2015) ncbi
EMD Millipore
rat monoclonal (M2-2-B3)
  • immunohistochemistry; mouse; 1:1000; fig 5c
In order to investigate cholinergic regulation of the olfactory bulb and examine neural morphology, EMD Millipore CHRM2 antibody (Millipore, AB367) was used in immunohistochemistry on mouse samples at 1:1000 (fig 5c). J Comp Neurol (2017) ncbi
rat monoclonal (M2-2-B3)
  • immunohistochemistry - frozen section; rat; 1:750; loading ...; fig 2
EMD Millipore CHRM2 antibody (Merck Millipore, MAB367) was used in immunohistochemistry - frozen section on rat samples at 1:750 (fig 2). J Comp Neurol (2016) ncbi
rat monoclonal (M2-2-B3)
  • immunohistochemistry; mouse; 1:500; fig 1
In order to study neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons by Neuregulin 1, EMD Millipore CHRM2 antibody (Merck Millipore, MAB367) was used in immunohistochemistry on mouse samples at 1:500 (fig 1). Acta Neuropathol Commun (2016) ncbi
rat monoclonal (M2-2-B3)
  • immunohistochemistry - frozen section; mouse
EMD Millipore CHRM2 antibody (Millipore, MAB367) was used in immunohistochemistry - frozen section on mouse samples . J Neurosci (2013) ncbi
rat monoclonal (M2-2-B3)
  • immunohistochemistry - paraffin section; mouse; 1:500
EMD Millipore CHRM2 antibody (Millipore, MAB367) was used in immunohistochemistry - paraffin section on mouse samples at 1:500. J Comp Neurol (2013) ncbi
rat monoclonal (M2-2-B3)
  • immunohistochemistry; tiger salamander; 1:100
EMD Millipore CHRM2 antibody (Chemicon, MAB367) was used in immunohistochemistry on tiger salamander samples at 1:100. J Comp Neurol (2008) ncbi
rat monoclonal (M2-2-B3)
  • immunohistochemistry - free floating section; Rhesus monkey; 1:2000
EMD Millipore CHRM2 antibody (Chemicon, MAB367) was used in immunohistochemistry - free floating section on Rhesus monkey samples at 1:2000. J Comp Neurol (2007) ncbi
rat monoclonal (M2-2-B3)
  • immunohistochemistry - frozen section; rat; 1:100
EMD Millipore CHRM2 antibody (Chemicon, MAB367) was used in immunohistochemistry - frozen section on rat samples at 1:100. J Comp Neurol (2007) ncbi
Articles Reviewed
  1. O Hern P, do Carmo G Gonçalves I, Brecht J, López Soto E, Simon J, Chapkis N, et al. Decreased microRNA levels lead to deleterious increases in neuronal M2 muscarinic receptors in Spinal Muscular Atrophy models. elife. 2017;6: pubmed publisher
  2. Hamamoto M, Kiyokage E, Sohn J, Hioki H, Harada T, Toida K. Structural basis for cholinergic regulation of neural circuits in the mouse olfactory bulb. J Comp Neurol. 2017;525:574-591 pubmed publisher
  3. Zhao Z, Azad R, Yang J, Siroky M, Azadzoi K. Progressive changes in detrusor function and micturition patterns with chronic bladder ischemia. Investig Clin Urol. 2016;57:249-59 pubmed publisher
  4. Olsen G, Witter M. Posterior parietal cortex of the rat: Architectural delineation and thalamic differentiation. J Comp Neurol. 2016;524:3774-3809 pubmed publisher
  5. Chen S, Kan Y, Zhang J, Wang J, Gao Y, Qiao L, et al. Involvement of hippocampal acetylcholinergic receptors in electroacupuncture analgesia in neuropathic pain rats. Behav Brain Funct. 2016;12:13 pubmed publisher
  6. Zhao Z, Zhang K, Liu X, Yan H, Ma X, Zhang S, et al. Involvement of HCN Channel in Muscarinic Inhibitory Action on Tonic Firing of Dorsolateral Striatal Cholinergic Interneurons. Front Cell Neurosci. 2016;10:71 pubmed publisher
  7. Bragança B, Oliveira Monteiro N, Ferreirinha F, Lima P, Faria M, Fontes Sousa A, et al. Ion Fluxes through KCa2 (SK) and Cav1 (L-type) Channels Contribute to Chronoselectivity of Adenosine A1 Receptor-Mediated Actions in Spontaneously Beating Rat Atria. Front Pharmacol. 2016;7:45 pubmed publisher
  8. Lasiene J, Komine O, Fujimori Tonou N, Powers B, Endo F, Watanabe S, et al. Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons. Acta Neuropathol Commun. 2016;4:15 pubmed publisher
  9. Wagner F, Bernard R, Derst C, French L, Veh R. Microarray analysis of transcripts with elevated expressions in the rat medial or lateral habenula suggest fast GABAergic excitation in the medial habenula and habenular involvement in the regulation of feeding and energy balance. Brain Struct Funct. 2016;221:4663-4689 pubmed
  10. Fornai M, Pellegrini C, Antonioli L, Segnani C, Ippolito C, Barocelli E, et al. Enteric Dysfunctions in Experimental Parkinson's Disease: Alterations of Excitatory Cholinergic Neurotransmission Regulating Colonic Motility in Rats. J Pharmacol Exp Ther. 2016;356:434-44 pubmed publisher
  11. Donovan C, Seow H, Royce S, Bourke J, Vlahos R. Alteration of Airway Reactivity and Reduction of Ryanodine Receptor Expression by Cigarette Smoke in Mice. Am J Respir Cell Mol Biol. 2015;53:471-8 pubmed publisher
  12. Cheng Y, Cao A, Zheng J, Wang H, Sun Y, Liu C, et al. Airway hyperresponsiveness induced by repeated esophageal infusion of HCl in guinea pigs. Am J Respir Cell Mol Biol. 2014;51:701-8 pubmed publisher
  13. Yang W, Carrasquillo Y, Hooks B, Nerbonne J, Burkhalter A. Distinct balance of excitation and inhibition in an interareal feedforward and feedback circuit of mouse visual cortex. J Neurosci. 2013;33:17373-84 pubmed publisher
  14. Ferretti M, Fabbiano C, Di Bari M, Conte C, Castigli E, Sciaccaluga M, et al. M2 receptor activation inhibits cell cycle progression and survival in human glioblastoma cells. J Cell Mol Med. 2013;17:552-66 pubmed publisher
  15. Csaba Z, Krejci E, Bernard V. Postsynaptic muscarinic m2 receptors at cholinergic and glutamatergic synapses of mouse brainstem motoneurons. J Comp Neurol. 2013;521:2008-24 pubmed publisher
  16. Cimini B, Strang C, Wotring V, Keyser K, Eldred W. Role of acetylcholine in nitric oxide production in the salamander retina. J Comp Neurol. 2008;507:1952-63 pubmed publisher
  17. Saleem K, Price J, Hashikawa T. Cytoarchitectonic and chemoarchitectonic subdivisions of the perirhinal and parahippocampal cortices in macaque monkeys. J Comp Neurol. 2007;500:973-1006 pubmed
  18. Jongen J, Jaarsma D, Hossaini M, Natarajan D, Haasdijk E, Holstege J. Distribution of RET immunoreactivity in the rodent spinal cord and changes after nerve injury. J Comp Neurol. 2007;500:1136-53 pubmed