This is a Validated Antibody Database (VAD) review about human CHD8, based on 10 published articles (read how Labome selects the articles), using CHD8 antibody in all methods. It is aimed to help Labome visitors find the most suited CHD8 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CHD8 synonym: AUTS18; HELSNF1; chromodomain-helicase-DNA-binding protein 8; ATP-dependent helicase CHD8; axis duplication inhibitor; duplin; helicase with SNF2 domain 1

Knockout validation
Bethyl
rabbit polyclonal
  • western blot knockout validation; human; fig 1
Bethyl CHD8 antibody (Bethyl Cat, A301-224A) was used in western blot knockout validation on human samples (fig 1). Mol Autism (2015) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry; human; 1:1000; loading ...; fig s6e
Abcam CHD8 antibody (Abcam, ab114126) was used in immunohistochemistry on human samples at 1:1000 (fig s6e). Dev Cell (2018) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 1h
  • immunohistochemistry; mouse; loading ...; fig 1b
  • western blot; mouse; loading ...; fig 1g
Abcam CHD8 antibody (Abcam, ab84527) was used in ChIP-Seq on mouse samples (fig 1h), in immunohistochemistry on mouse samples (fig 1b) and in western blot on mouse samples (fig 1g). Dev Cell (2018) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse; fig 5
  • western blot; mouse; fig s3
Abcam CHD8 antibody (abcam, ab84527) was used in chromatin immunoprecipitation on mouse samples (fig 5) and in western blot on mouse samples (fig s3). Nucleic Acids Res (2016) ncbi
rabbit polyclonal
  • ChIP-Seq; human; fig 1
  • immunoprecipitation; human; fig s2
  • western blot; human; fig 4
Abcam CHD8 antibody (Abcam, ab114126) was used in ChIP-Seq on human samples (fig 1), in immunoprecipitation on human samples (fig s2) and in western blot on human samples (fig 4). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human
Abcam CHD8 antibody (Abcam, ab84527) was used in immunohistochemistry - paraffin section on human samples . Gastroenterology (2014) ncbi
rabbit polyclonal
  • immunoprecipitation; human
  • western blot; human; 1:2000
  • immunohistochemistry; mouse; 1:100
  • western blot; mouse; 1:2000
Abcam CHD8 antibody (Abcam, ab84527) was used in immunoprecipitation on human samples , in western blot on human samples at 1:2000, in immunohistochemistry on mouse samples at 1:100 and in western blot on mouse samples at 1:2000. PLoS ONE (2012) ncbi
Bethyl
rabbit polyclonal
  • western blot knockout validation; human; fig 1
Bethyl CHD8 antibody (Bethyl Cat, A301-224A) was used in western blot knockout validation on human samples (fig 1). Mol Autism (2015) ncbi
rabbit polyclonal
  • ChIP-Seq; human; fig 4
  • chromatin immunoprecipitation; human; fig 6
  • immunoprecipitation; human; fig 6
  • western blot; human; fig 6
Bethyl CHD8 antibody (Bethyl Laboratories, A301-224A) was used in ChIP-Seq on human samples (fig 4), in chromatin immunoprecipitation on human samples (fig 6), in immunoprecipitation on human samples (fig 6) and in western blot on human samples (fig 6). PLoS Genet (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • western blot; human; 1:2000; fig 1d
In order to examine the transcriptional networks that CHD8 regulates in neural progenitor cells, Bethyl CHD8 antibody (Bethyl, A301-224A) was used in chromatin immunoprecipitation on human samples and in western blot on human samples at 1:2000 (fig 1d). Proc Natl Acad Sci U S A (2014) ncbi
Novus Biologicals
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • western blot; human; 1:500; fig 1d
In order to examine the transcriptional networks that CHD8 regulates in neural progenitor cells, Novus Biologicals CHD8 antibody (Novus, NB100-60417) was used in chromatin immunoprecipitation on human samples and in western blot on human samples at 1:500 (fig 1d). Proc Natl Acad Sci U S A (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • western blot; human; 1:500; fig 1d
In order to examine the transcriptional networks that CHD8 regulates in neural progenitor cells, Novus Biologicals CHD8 antibody (Novus, NB100-60418) was used in chromatin immunoprecipitation on human samples and in western blot on human samples at 1:500 (fig 1d). Proc Natl Acad Sci U S A (2014) ncbi
Abnova
mouse polyclonal
  • immunocytochemistry; human
In order to evaluate the BioID proximity-based biotin labeling technique for the identification of novel c-MYC interacting partners in cultured cells and xenograft tumors, Abnova CHD8 antibody (Abnova, H00057680-A01) was used in immunocytochemistry on human samples . J Proteomics (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D3C1)
  • western blot; human; loading ...; fig 2c
In order to describe how Hic-5 regulates glucocorticoid receptor binding site selection, Cell Signaling Technology CHD8 antibody (Cell Signaling, 11891S) was used in western blot on human samples (fig 2c). J Biol Chem (2017) ncbi
rabbit monoclonal (D3C1)
  • immunoprecipitation; human; fig s2
  • western blot; human; fig s2
Cell Signaling Technology CHD8 antibody (Cell Signaling, 11891S) was used in immunoprecipitation on human samples (fig s2) and in western blot on human samples (fig s2). Nat Commun (2015) ncbi
Articles Reviewed
  1. Zhao C, Dong C, Frah M, Deng Y, Marie C, Zhang F, et al. Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair. Dev Cell. 2018;45:753-768.e8 pubmed publisher
  2. Lee B, Stallcup M. Glucocorticoid receptor binding to chromatin is selectively controlled by the coregulator Hic-5 and chromatin remodeling enzymes. J Biol Chem. 2017;292:9320-9334 pubmed publisher
  3. Wang P, Lin M, Pedrosa E, Hrabovsky A, Zhang Z, Guo W, et al. CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in neurodevelopment. Mol Autism. 2015;6:55 pubmed publisher
  4. Akhade V, Dighe S, Kataruka S, Rao M. Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells. Nucleic Acids Res. 2016;44:387-401 pubmed publisher
  5. Ceballos Chávez M, Subtil Rodríguez A, Giannopoulou E, Soronellas D, Vázquez Chávez E, Vicent G, et al. The chromatin Remodeler CHD8 is required for activation of progesterone receptor-dependent enhancers. PLoS Genet. 2015;11:e1005174 pubmed publisher
  6. Cotney J, Muhle R, Sanders S, Liu L, Willsey A, Niu W, et al. The autism-associated chromatin modifier CHD8 regulates other autism risk genes during human neurodevelopment. Nat Commun. 2015;6:6404 pubmed publisher
  7. Dingar D, Kalkat M, Chan P, Srikumar T, Bailey S, Tu W, et al. BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors. J Proteomics. 2015;118:95-111 pubmed publisher
  8. Sugathan A, Biagioli M, Golzio C, Erdin S, Blumenthal I, Manavalan P, et al. CHD8 regulates neurodevelopmental pathways associated with autism spectrum disorder in neural progenitors. Proc Natl Acad Sci U S A. 2014;111:E4468-77 pubmed publisher
  9. Tahara T, Yamamoto E, Madireddi P, Suzuki H, Maruyama R, Chung W, et al. Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators. Gastroenterology. 2014;146:530-38.e5 pubmed publisher
  10. Batsukh T, Schulz Y, Wolf S, Rabe T, Oellerich T, Urlaub H, et al. Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex. PLoS ONE. 2012;7:e52640 pubmed publisher