This is a Validated Antibody Database (VAD) review about human CHD7, based on 6 published articles (read how Labome selects the articles), using CHD7 antibody in all methods. It is aimed to help Labome visitors find the most suited CHD7 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CHD7 synonym: CRG; HH5; IS3; KAL5

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D3F5)
  • immunohistochemistry knockout validation; mouse; 1:2000; fig s1b
  • western blot knockout validation; mouse; 1:1000; fig s1c
Cell Signaling Technology CHD7 antibody (Cell Signaling Technology, 6505S) was used in immunohistochemistry knockout validation on mouse samples at 1:2000 (fig s1b) and in western blot knockout validation on mouse samples at 1:1000 (fig s1c). Nat Commun (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D3F5)
  • western blot; human; 1:400; loading ...; fig 5c
  • immunohistochemistry knockout validation; mouse; 1:400; loading ...; fig s3a
  • ChIP-Seq; mouse; loading ...; fig 4e
  • immunohistochemistry - paraffin section; mouse; 1:400; loading ...; fig 1a
In order to explore the role of chromodomain helicase DNA-binding protein 7 in CHARGE syndrome, Cell Signaling Technology CHD7 antibody (Cell Signaling, 6505) was used in western blot on human samples at 1:400 (fig 5c), in immunohistochemistry knockout validation on mouse samples at 1:400 (fig s3a), in ChIP-Seq on mouse samples (fig 4e) and in immunohistochemistry - paraffin section on mouse samples at 1:400 (fig 1a). Nat Commun (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D3F5)
  • immunohistochemistry knockout validation; mouse; 1:2000; fig s1b
  • western blot knockout validation; mouse; 1:1000; fig s1c
Cell Signaling Technology CHD7 antibody (Cell Signaling Technology, 6505S) was used in immunohistochemistry knockout validation on mouse samples at 1:2000 (fig s1b) and in western blot knockout validation on mouse samples at 1:1000 (fig s1c). Nat Commun (2021) ncbi
domestic rabbit monoclonal (D3F5)
  • ChIP-Seq; mouse; loading ...; fig 1i
  • western blot; mouse; loading ...; fig 1g
Cell Signaling Technology CHD7 antibody (Cell Signaling, 6505) was used in ChIP-Seq on mouse samples (fig 1i) and in western blot on mouse samples (fig 1g). Dev Cell (2018) ncbi
domestic rabbit monoclonal (D3F5)
  • western blot; human; loading ...; fig 2c
In order to describe how Hic-5 regulates glucocorticoid receptor binding site selection, Cell Signaling Technology CHD7 antibody (Cell Signaling, 6505S) was used in western blot on human samples (fig 2c). J Biol Chem (2017) ncbi
domestic rabbit monoclonal (D3F5)
  • western blot; human; 1:400; loading ...; fig 5c
  • immunohistochemistry knockout validation; mouse; 1:400; loading ...; fig s3a
  • ChIP-Seq; mouse; loading ...; fig 4e
  • immunohistochemistry - paraffin section; mouse; 1:400; loading ...; fig 1a
In order to explore the role of chromodomain helicase DNA-binding protein 7 in CHARGE syndrome, Cell Signaling Technology CHD7 antibody (Cell Signaling, 6505) was used in western blot on human samples at 1:400 (fig 5c), in immunohistochemistry knockout validation on mouse samples at 1:400 (fig s3a), in ChIP-Seq on mouse samples (fig 4e) and in immunohistochemistry - paraffin section on mouse samples at 1:400 (fig 1a). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D3F5)
  • immunohistochemistry; mouse; loading ...; fig 6e
Cell Signaling Technology CHD7 antibody (Cell Signaling, 6505) was used in immunohistochemistry on mouse samples (fig 6e). Mol Biol Cell (2017) ncbi
domestic rabbit monoclonal (D3F5)
  • ChIP-Seq; mouse; fig 1a
Cell Signaling Technology CHD7 antibody (Cell signaling, 6505) was used in ChIP-Seq on mouse samples (fig 1a). Nat Neurosci (2016) ncbi
Articles Reviewed
  1. Reddy N, Majidi S, Kong L, Nemera M, Ferguson C, Moore M, et al. CHARGE syndrome protein CHD7 regulates epigenomic activation of enhancers in granule cell precursors and gyrification of the cerebellum. Nat Commun. 2021;12:5702 pubmed publisher
  2. Zhao C, Dong C, Frah M, Deng Y, Marie C, Zhang F, et al. Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair. Dev Cell. 2018;45:753-768.e8 pubmed publisher
  3. Lee B, Stallcup M. Glucocorticoid receptor binding to chromatin is selectively controlled by the coregulator Hic-5 and chromatin remodeling enzymes. J Biol Chem. 2017;292:9320-9334 pubmed publisher
  4. Feng W, Kawauchi D, Körkel Qu H, Deng H, Serger E, Sieber L, et al. Chd7 is indispensable for mammalian brain development through activation of a neuronal differentiation programme. Nat Commun. 2017;8:14758 pubmed publisher
  5. Bird J, Barzik M, Drummond M, Sutton D, Goodman S, Morozko E, et al. Harnessing molecular motors for nanoscale pulldown in live cells. Mol Biol Cell. 2017;28:463-475 pubmed publisher
  6. He D, Marie C, Zhao C, Kim B, Wang J, Deng Y, et al. Chd7 cooperates with Sox10 and regulates the onset of CNS myelination and remyelination. Nat Neurosci. 2016;19:678-89 pubmed publisher