This is a Validated Antibody Database (VAD) review about human CHD4, based on 20 published articles (read how Labome selects the articles), using CHD4 antibody in all methods. It is aimed to help Labome visitors find the most suited CHD4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CHD4 synonym: CHD-4; Mi-2b; Mi2-BETA; SIHIWES

Abcam
mouse monoclonal (3F2/4)
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s5a
  • western blot; mouse; 1:1000; loading ...; fig 5b
Abcam CHD4 antibody (Abcam, ab70469) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig s5a) and in western blot on mouse samples at 1:1000 (fig 5b). Sci Adv (2022) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; mouse; loading ...; fig 1c
Abcam CHD4 antibody (Abcam, ab72418) was used in immunoprecipitation on mouse samples (fig 1c). Epigenetics Chromatin (2022) ncbi
mouse monoclonal (3F2/4)
  • ChIP-Seq; mouse; loading ...; fig 5b
  • immunohistochemistry - paraffin section; mouse; 1:300; loading ...; fig 1a
Abcam CHD4 antibody (Abcam, ab70469) was used in ChIP-Seq on mouse samples (fig 5b) and in immunohistochemistry - paraffin section on mouse samples at 1:300 (fig 1a). Epigenetics Chromatin (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; loading ...
  • western blot; mouse; 1:1000; loading ...
Abcam CHD4 antibody (Abcam, ab72418) was used in immunohistochemistry on mouse samples at 1:1000 and in western blot on mouse samples at 1:1000. Sci Rep (2021) ncbi
mouse monoclonal (3F2/4)
  • western blot; mouse; loading ...
Abcam CHD4 antibody (Abcam, ab70469) was used in western blot on mouse samples . Cell Stem Cell (2020) ncbi
mouse monoclonal (3F2/4)
  • western blot; mouse; loading ...; fig s1b
Abcam CHD4 antibody (Abcam, ab70469) was used in western blot on mouse samples (fig s1b). Cell Rep (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:250; loading ...; fig 2a
Abcam CHD4 antibody (Abcam, ab72418) was used in western blot on human samples at 1:250 (fig 2a). Sci Rep (2019) ncbi
mouse monoclonal (3F2/4)
  • western blot; mouse; loading ...; fig 1c
Abcam CHD4 antibody (Abcam, ab70469) was used in western blot on mouse samples (fig 1c). Mol Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2c
In order to describe how Hic-5 regulates glucocorticoid receptor binding site selection, Abcam CHD4 antibody (Abcam, ab72418) was used in western blot on human samples (fig 2c). J Biol Chem (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4a
Abcam CHD4 antibody (Abcam, AB72418) was used in western blot on human samples (fig 4a). elife (2017) ncbi
mouse monoclonal (3F2/4)
  • chromatin immunoprecipitation; human; loading ...; fig 4d
Abcam CHD4 antibody (Abcam, AB70469) was used in chromatin immunoprecipitation on human samples (fig 4d). elife (2017) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig s1c
Abcam CHD4 antibody (Abcam, ab72418) was used in ChIP-Seq on mouse samples (fig s1c). Science (2016) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; fig 5
Abcam CHD4 antibody (Abcam, ab72418) was used in ChIP-Seq on mouse samples (fig 5). Cell Metab (2016) ncbi
mouse monoclonal (3F2/4)
  • western blot; mouse; fig 8d
In order to study transcription factor Blimp-1 in coordinating plasma cell differentiation, Abcam CHD4 antibody (Abcam, 3F2/4) was used in western blot on mouse samples (fig 8d). Nat Immunol (2016) ncbi
Active Motif
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...
Active Motif CHD4 antibody (Active Motif, 39289) was used in immunohistochemistry on mouse samples at 1:200. Sci Rep (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:200; loading ...; fig 3c
Active Motif CHD4 antibody (Active Motif, 39289) was used in western blot on human samples at 1:200 (fig 3c). EMBO Mol Med (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig s3c
Active Motif CHD4 antibody (Active Motif, 39289) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig s3c). J Clin Invest (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:500; fig 5k
In order to report that the TBX5-NuRD interaction is essential for cardiac development, Active Motif CHD4 antibody (Active Motif, 39,289) was used in western blot on mouse samples at 1:500 (fig 5k). Dev Cell (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-4)
  • western blot; human; loading ...; fig s1
Santa Cruz Biotechnology CHD4 antibody (Santa Cruz Biotechnology, sc-55606) was used in western blot on human samples (fig s1). Nucleic Acids Res (2017) ncbi
Abnova
mouse monoclonal (4H4)
  • chromatin immunoprecipitation; human; fig 6f
  • western blot; human; fig 1e
Abnova CHD4 antibody (Abnova, H00001108) was used in chromatin immunoprecipitation on human samples (fig 6f) and in western blot on human samples (fig 1e). J Biol Chem (2014) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4B7)
  • immunohistochemistry - paraffin section; human; 1:400; loading ...; fig 6b
  • chromatin immunoprecipitation; human; 1:50; loading ...; fig 6d
Cell Signaling Technology CHD4 antibody (CST, 12011s) was used in immunohistochemistry - paraffin section on human samples at 1:400 (fig 6b) and in chromatin immunoprecipitation on human samples at 1:50 (fig 6d). Br J Cancer (2022) ncbi
domestic rabbit monoclonal (D4B7)
  • western blot; human; 1:1000; fig 5e
Cell Signaling Technology CHD4 antibody (Cell Signaling Technology, 12011S) was used in western blot on human samples at 1:1000 (fig 5e). Cancer Res (2021) ncbi
domestic rabbit monoclonal (D8B12)
  • western blot; mouse; loading ...; fig 1a
Cell Signaling Technology CHD4 antibody (CST, 11912) was used in western blot on mouse samples (fig 1a). Cell Rep (2019) ncbi
domestic rabbit monoclonal (D4B7)
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology CHD4 antibody (CST, 12011) was used in western blot on human samples (fig 2c). Genes Dev (2017) ncbi
Articles Reviewed
  1. Ma L, Xie D, Luo M, Lin X, Nie H, Chen J, et al. Identification and characterization of BEND2 as a key regulator of meiosis during mouse spermatogenesis. Sci Adv. 2022;8:eabn1606 pubmed publisher
  2. de Castro R, Carbajal A, Previato de Almeida L, Goitea V, Griffin C, Pezza R. Mouse Chd4-NURD is required for neonatal spermatogonia survival and normal gonad development. Epigenetics Chromatin. 2022;15:16 pubmed publisher
  3. Yan W, Han Q, Gong L, Zhan X, Li W, Guo Z, et al. MBD3 promotes hepatocellular carcinoma progression and metastasis through negative regulation of tumour suppressor TFPI2. Br J Cancer. 2022;: pubmed publisher
  4. Zhao Z, Szczepanski A, Tsuboyama N, Abdala Valencia H, Goo Y, Singer B, et al. PAX9 Determines Epigenetic State Transition and Cell Fate in Cancer. Cancer Res. 2021;81:4696-4708 pubmed publisher
  5. Mattar P, Jolicoeur C, Dang T, Shah S, Clark B, Cayouette M. A Casz1-NuRD complex regulates temporal identity transitions in neural progenitors. Sci Rep. 2021;11:3858 pubmed publisher
  6. Yang F, Huang X, Zang R, Chen J, Fidalgo M, Sanchez Priego C, et al. DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State. Cell Stem Cell. 2020;26:234-250.e7 pubmed publisher
  7. Tacconi E, Badie S, De Gregoriis G, Reisländer T, Lai X, Porru M, et al. Chlorambucil targets BRCA1/2-deficient tumours and counteracts PARP inhibitor resistance. EMBO Mol Med. 2019;11:e9982 pubmed publisher
  8. Yen W, Sharma R, Cols M, Lau C, Chaudhry A, Chowdhury P, et al. Distinct Requirements of CHD4 during B Cell Development and Antibody Response. Cell Rep. 2019;27:1472-1486.e5 pubmed publisher
  9. McKenzie L, LeClair J, Miller K, Strong A, Chan H, Oates E, et al. CHD4 regulates the DNA damage response and RAD51 expression in glioblastoma. Sci Rep. 2019;9:4444 pubmed publisher
  10. Bornelöv S, Reynolds N, Xenophontos M, Gharbi S, Johnstone E, Floyd R, et al. The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression. Mol Cell. 2018;71:56-72.e4 pubmed publisher
  11. Morgan M, Rickels R, Collings C, He X, Cao K, Herz H, et al. A cryptic Tudor domain links BRWD2/PHIP to COMPASS-mediated histone H3K4 methylation. Genes Dev. 2017;31:2003-2014 pubmed publisher
  12. Blank M, Chen S, Poetz F, Schnolzer M, Voit R, Grummt I. SIRT7-dependent deacetylation of CDK9 activates RNA polymerase II transcription. Nucleic Acids Res. 2017;45:2675-2686 pubmed publisher
  13. Lee B, Stallcup M. Glucocorticoid receptor binding to chromatin is selectively controlled by the coregulator Hic-5 and chromatin remodeling enzymes. J Biol Chem. 2017;292:9320-9334 pubmed publisher
  14. Liang Z, Brown K, Carroll T, Taylor B, Vidal I, Hendrich B, et al. A high-resolution map of transcriptional repression. elife. 2017;6: pubmed publisher
  15. Yang Y, Yamada T, Hill K, Hemberg M, Reddy N, Cho H, et al. Chromatin remodeling inactivates activity genes and regulates neural coding. Science. 2016;353:300-305 pubmed publisher
  16. Gómez del Arco P, Perdiguero E, Yunes Leites P, Acin Perez R, Zeini M, Garcia Gomez A, et al. The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis. Cell Metab. 2016;23:881-92 pubmed publisher
  17. Crosswhite P, Podsiadlowska J, Curtis C, Gao S, Xia L, Srinivasan R, et al. CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity. J Clin Invest. 2016;126:2254-66 pubmed publisher
  18. Waldron L, Steimle J, Greco T, Gomez N, Dorr K, Kweon J, et al. The Cardiac TBX5 Interactome Reveals a Chromatin Remodeling Network Essential for Cardiac Septation. Dev Cell. 2016;36:262-75 pubmed publisher
  19. Minnich M, Tagoh H, Bönelt P, Axelsson E, Fischer M, Cebolla B, et al. Multifunctional role of the transcription factor Blimp-1 in coordinating plasma cell differentiation. Nat Immunol. 2016;17:331-43 pubmed publisher
  20. Nishibuchi G, Shibata Y, Hayakawa T, Hayakawa N, Ohtani Y, Sinmyozu K, et al. Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex. J Biol Chem. 2014;289:28956-70 pubmed publisher