This is a Validated Antibody Database (VAD) review about human CFTR, based on 19 published articles (read how Labome selects the articles), using CFTR antibody in all methods. It is aimed to help Labome visitors find the most suited CFTR antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CFTR synonym: ABC35; ABCC7; CF; CFTR/MRP; MRP7; TNR-CFTR; dJ760C5.1

Knockout validation
Abcam
mouse monoclonal (CF3)
  • western blot knockout validation; mouse; 1:1000; fig 2
Abcam CFTR antibody (Abcam, ab2784) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2). Cell Death Differ (2016) ncbi
CFTR Folding Consortium
mouse monoclonal (3G11)
  • immunohistochemistry knockout validation; mouse; 1:500
CFTR Folding Consortium CFTR antibody (Cystic Fibrosis Folding Consortium, 3G11) was used in immunohistochemistry knockout validation on mouse samples at 1:500. PLoS ONE (2013) ncbi
CFTR Folding Consortium
mouse monoclonal (3G11)
  • western blot knockout validation; mouse; fig 3a
CFTR Folding Consortium CFTR antibody (Cystic Fibrosis Foundation Therapeutics, 3G11) was used in western blot knockout validation on mouse samples (fig 3a). Am J Physiol Cell Physiol (2012) ncbi
Abcam
mouse monoclonal (CF3)
  • immunohistochemistry; human; loading ...; fig 1b
Abcam CFTR antibody (Abcam, 2784) was used in immunohistochemistry on human samples (fig 1b). Sci Rep (2018) ncbi
mouse monoclonal (CF3)
  • immunocytochemistry; human; fig 2i
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5b
Abcam CFTR antibody (Abcam, CF3) was used in immunocytochemistry on human samples (fig 2i) and in immunohistochemistry - paraffin section on mouse samples (fig 5b). Nat Med (2017) ncbi
mouse monoclonal (CF3)
  • western blot; mouse; 1:500; fig 3b
  • western blot; human; 1:500; fig 1b
Abcam CFTR antibody (Abcam, ab2784) was used in western blot on mouse samples at 1:500 (fig 3b) and in western blot on human samples at 1:500 (fig 1b). Exp Physiol (2016) ncbi
mouse monoclonal (CF3)
  • western blot knockout validation; mouse; 1:1000; fig 2
Abcam CFTR antibody (Abcam, ab2784) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2). Cell Death Differ (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; tbl 1
  • immunohistochemistry - paraffin section; mouse; 1:200; tbl 1
Abcam CFTR antibody (Abcam, ab59394) was used in immunohistochemistry - paraffin section on human samples at 1:200 (tbl 1) and in immunohistochemistry - paraffin section on mouse samples at 1:200 (tbl 1). Sci Rep (2016) ncbi
mouse monoclonal (CF3)
  • western blot; human; fig s2
Abcam CFTR antibody (Abcam, ab2784) was used in western blot on human samples (fig s2). PLoS ONE (2016) ncbi
mouse monoclonal (CF3)
  • flow cytometry; human; 1:20; fig 1b
Abcam CFTR antibody (Abcam, CF3) was used in flow cytometry on human samples at 1:20 (fig 1b). Curr Cancer Drug Targets (2016) ncbi
mouse monoclonal (CF3)
  • immunohistochemistry; human; fig 3
Abcam CFTR antibody (Abcam, ab2784) was used in immunohistochemistry on human samples (fig 3). Exp Cell Res (2015) ncbi
mouse monoclonal (CF3)
  • immunohistochemistry; pigs ; 1:50
Abcam CFTR antibody (Abcam, ab2784) was used in immunohistochemistry on pigs samples at 1:50. Br J Pharmacol (2014) ncbi
R&D Systems
mouse monoclonal (24-1)
  • immunocytochemistry; human; 1:100
In order to determine if administration of resveratrol is beneficial for treating cystic fibrosis, R&D Systems CFTR antibody (R&D Systems, MAB25031) was used in immunocytochemistry on human samples at 1:100. Biol Open (2015) ncbi
mouse monoclonal (24-1)
  • immunohistochemistry - frozen section; mouse; fig 3
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 5
R&D Systems CFTR antibody (R&D Systems, 24-1) was used in immunohistochemistry - frozen section on mouse samples (fig 3), in immunocytochemistry on human samples (fig 1) and in western blot on human samples (fig 5). Methods Mol Biol (2015) ncbi
mouse monoclonal (24-1)
  • immunoprecipitation; human; 1:100
R&D Systems CFTR antibody (R&D Systems, 24-1) was used in immunoprecipitation on human samples at 1:100. Biochim Biophys Acta (2015) ncbi
mouse monoclonal (13-1)
  • western blot; human; 1:50
In order to describe a method to generate cystic fibrosis transmembrane conductance regulator protein-expressing airway epithelial cells from human pluripotent stem cells, R&D Systems CFTR antibody (R&D Systems, MAB1660) was used in western blot on human samples at 1:50. Nat Protoc (2015) ncbi
mouse monoclonal (24-1)
  • western blot; human; 1:400
R&D Systems CFTR antibody (R&D Systems, MAB25031) was used in western blot on human samples at 1:400. PLoS ONE (2014) ncbi
mouse monoclonal (24-1)
  • western blot; human; 0.4 ug/ml
In order to investigate the role of gap junctional communication in Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells, R&D Systems CFTR antibody (R&D Systems, MAB25031) was used in western blot on human samples at 0.4 ug/ml. J Immunol (2014) ncbi
mouse monoclonal (13-1)
  • immunocytochemistry; human; 1:100
R&D Systems CFTR antibody (R&D Systems, MAB1660) was used in immunocytochemistry on human samples at 1:100. Am J Physiol Cell Physiol (2013) ncbi
Proteintech Group
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:2500; loading ...; fig 2g
Proteintech Group CFTR antibody (Proteintech, 20738-1-AP) was used in immunohistochemistry - paraffin section on mouse samples at 1:2500 (fig 2g). Nat Commun (2020) ncbi
CFTR Folding Consortium
mouse monoclonal (3G11)
  • immunohistochemistry knockout validation; mouse; 1:500
CFTR Folding Consortium CFTR antibody (Cystic Fibrosis Folding Consortium, 3G11) was used in immunohistochemistry knockout validation on mouse samples at 1:500. PLoS ONE (2013) ncbi
mouse monoclonal (3G11)
  • western blot knockout validation; mouse; fig 3a
CFTR Folding Consortium CFTR antibody (Cystic Fibrosis Foundation Therapeutics, 3G11) was used in western blot knockout validation on mouse samples (fig 3a). Am J Physiol Cell Physiol (2012) ncbi
Articles Reviewed
  1. Perkail S, Andricovich J, Kai Y, Tzatsos A. BAP1 is a haploinsufficient tumor suppressor linking chronic pancreatitis to pancreatic cancer in mice. Nat Commun. 2020;11:3018 pubmed publisher
  2. Zhang S, Shrestha C, Kopp B. Cystic fibrosis transmembrane conductance regulator (CFTR) modulators have differential effects on cystic fibrosis macrophage function. Sci Rep. 2018;8:17066 pubmed publisher
  3. Romani L, Oikonomou V, Moretti S, Iannitti R, D Adamo M, Villella V, et al. Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis. Nat Med. 2017;23:590-600 pubmed publisher
  4. Jin H, Wen G, Deng S, Wan S, Xu J, Liu X, et al. Oestrogen upregulates the expression levels and functional activities of duodenal mucosal CFTR and SLC26A6. Exp Physiol. 2016;101:1371-1382 pubmed publisher
  5. Tosco A, De Gregorio F, Esposito S, De Stefano D, Sana I, Ferrari E, et al. A novel treatment of cystic fibrosis acting on-target: cysteamine plus epigallocatechin gallate for the autophagy-dependent rescue of class II-mutated CFTR. Cell Death Differ. 2016;23:1380-93 pubmed publisher
  6. Brennan S, Wilkinson W, Tseng H, Finney B, Monk B, Dibble H, et al. The extracellular calcium-sensing receptor regulates human fetal lung development via CFTR. Sci Rep. 2016;6:21975 pubmed publisher
  7. Khoory J, Estanislau J, Elkhal A, Lazaar A, Melhorn M, Brodsky A, et al. Ligation of Glycophorin A Generates Reactive Oxygen Species Leading to Decreased Red Blood Cell Function. PLoS ONE. 2016;11:e0141206 pubmed publisher
  8. Fanelli M, Hattinger C, Vella S, Tavanti E, Michelacci F, Gudeman B, et al. Targeting ABCB1 and ABCC1 with their Specific Inhibitor CBT-1® can Overcome Drug Resistance in Osteosarcoma. Curr Cancer Drug Targets. 2016;16:261-74 pubmed
  9. Dhooghe B, Bouckaert C, Capron A, Wallemacq P, Leal T, Noel S. Resveratrol increases F508del-CFTR dependent salivary secretion in cystic fibrosis mice. Biol Open. 2015;4:929-36 pubmed publisher
  10. Gee H, Kim J, Lee M. Analysis of conventional and unconventional trafficking of CFTR and other membrane proteins. Methods Mol Biol. 2015;1270:137-54 pubmed publisher
  11. Philippe R, Antigny F, Buscaglia P, Norez C, Becq F, Frieden M, et al. SERCA and PMCA pumps contribute to the deregulation of Ca2+ homeostasis in human CF epithelial cells. Biochim Biophys Acta. 2015;1853:892-903 pubmed publisher
  12. Wong A, Chin S, Xia S, Garner J, Bear C, Rossant J. Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells. Nat Protoc. 2015;10:363-81 pubmed publisher
  13. Li H, Chen L, Zeng S, Li X, Zhang X, Lin C, et al. Matrigel basement membrane matrix induces eccrine sweat gland cells to reconstitute sweat gland-like structures in nude mice. Exp Cell Res. 2015;332:67-77 pubmed publisher
  14. Zhao R, Liang X, Zhao M, Liu S, Huang Y, Idell S, et al. Correlation of apical fluid-regulating channel proteins with lung function in human COPD lungs. PLoS ONE. 2014;9:e109725 pubmed publisher
  15. Losa D, Köhler T, Bellec J, Dudez T, Crespin S, Bacchetta M, et al. Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner. J Immunol. 2014;192:4804-12 pubmed publisher
  16. McLatchie L, Young J, Fry C. Regulation of ACh release from guinea pig bladder urothelial cells: potential role in bladder filling sensations. Br J Pharmacol. 2014;171:3394-403 pubmed publisher
  17. Stalvey M, Clines K, Havasi V, McKibbin C, Dunn L, Chung W, et al. Osteoblast CFTR inactivation reduces differentiation and osteoprotegerin expression in a mouse model of cystic fibrosis-related bone disease. PLoS ONE. 2013;8:e80098 pubmed publisher
  18. Wang J, Haanes K, Novak I. Purinergic regulation of CFTR and Ca(2+)-activated Cl(-) channels and K(+) channels in human pancreatic duct epithelium. Am J Physiol Cell Physiol. 2013;304:C673-84 pubmed publisher
  19. Liu J, Walker N, Cook M, Ootani A, Clarke L. Functional Cftr in crypt epithelium of organotypic enteroid cultures from murine small intestine. Am J Physiol Cell Physiol. 2012;302:C1492-503 pubmed publisher