This is a Validated Antibody Database (VAD) review about human CENPF, based on 8 published articles (read how Labome selects the articles), using CENPF antibody in all methods. It is aimed to help Labome visitors find the most suited CENPF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CENPF synonym: CENF; CILD31; PRO1779; STROMS; hcp-1

Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 2g, 2h
  • immunohistochemistry; human; fig 6g, s5d
Abcam CENPF antibody (Abcam, ab5) was used in immunohistochemistry - paraffin section on human samples (fig 2g, 2h) and in immunohistochemistry on human samples (fig 6g, s5d). Aging (Albany NY) (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 4
  • western blot; human; loading ...; fig s3
Abcam CENPF antibody (Abcam, ab5) was used in immunocytochemistry on human samples (fig 4) and in western blot on human samples (fig s3). Oncotarget (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; loading ...; fig 2b
In order to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair, Abcam CENPF antibody (Abcam, AB5) was used in immunohistochemistry on human samples (fig 2b). Mol Cell (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:2000; fig 4
Abcam CENPF antibody (Abcam, Ab5) was used in western blot on human samples at 1:2000 (fig 4). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:300; fig 7
  • western blot; human; 1:300; fig 9
In order to test if progerin elicits spatiotemporal deviations in mitotic processes in Hutchinson-Gilford progeria syndrome fibroblasts, Abcam CENPF antibody (Abcam, ab5) was used in immunocytochemistry on human samples at 1:300 (fig 7) and in western blot on human samples at 1:300 (fig 9). Oncotarget (2016) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:300; loading ...; fig 2f
  • western blot; human; 1:500; loading ...; fig 4g
Novus Biologicals CENPF antibody (Novus biologicals, NB500-101) was used in immunocytochemistry on human samples at 1:300 (fig 2f) and in western blot on human samples at 1:500 (fig 4g). J Biol Chem (2018) ncbi
BD Biosciences
mouse monoclonal (11/Mitosin)
  • immunohistochemistry; human; loading ...; fig 5b
In order to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair, BD Biosciences CENPF antibody (BD, 610768) was used in immunohistochemistry on human samples (fig 5b). Mol Cell (2017) ncbi
mouse monoclonal (11/Mitosin)
  • immunocytochemistry; human; 1:500; fig 1
BD Biosciences CENPF antibody (BD Biosciences, 610768) was used in immunocytochemistry on human samples at 1:500 (fig 1). Nat Commun (2015) ncbi
mouse monoclonal (11/Mitosin)
  • immunocytochemistry; human; 1:100
BD Biosciences CENPF antibody (BD Bioscience, 610768) was used in immunocytochemistry on human samples at 1:100. PLoS ONE (2013) ncbi
Articles Reviewed
  1. Hexiao T, Yuquan B, Lecai X, Yanhong W, Li S, Weidong H, et al. Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ERĪ²2/5 pathway. Aging (Albany NY). 2021;13:2604-2625 pubmed publisher
  2. Ciossani G, Overlack K, Petrovic A, Huis In t Veld P, Koerner C, Wohlgemuth S, et al. The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases. J Biol Chem. 2018;293:10084-10101 pubmed publisher
  3. Pagotto S, Veronese A, Soranno A, Lanuti P, Di Marco M, Russo M, et al. Hsa-miR-155-5p drives aneuploidy at early stages of cellular transformation. Oncotarget. 2018;9:13036-13047 pubmed publisher
  4. Clarke T, Sanchez Bailon M, Chiang K, Reynolds J, Herrero Ruiz J, Bandeiras T, et al. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Mol Cell. 2017;65:900-916.e7 pubmed publisher
  5. Lin S, Kao C, Lee H, Creighton C, Ittmann M, Tsai S, et al. Dysregulation of miRNAs-COUP-TFII-FOXM1-CENPF axis contributes to the metastasis of prostate cancer. Nat Commun. 2016;7:11418 pubmed publisher
  6. Eisch V, Lu X, Gabriel D, Djabali K. Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts. Oncotarget. 2016;7:24700-18 pubmed publisher
  7. Kanfer G, Courtheoux T, Peterka M, Meier S, Soste M, Melnik A, et al. Mitotic redistribution of the mitochondrial network by Miro and Cenp-F. Nat Commun. 2015;6:8015 pubmed publisher
  8. Bee L, Fabris S, Cherubini R, Mognato M, Celotti L. The efficiency of homologous recombination and non-homologous end joining systems in repairing double-strand breaks during cell cycle progression. PLoS ONE. 2013;8:e69061 pubmed publisher