This is a Validated Antibody Database (VAD) review about human CENPE, based on 7 published articles (read how Labome selects the articles), using CENPE antibody in all methods. It is aimed to help Labome visitors find the most suited CENPE antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CENPE synonym: CENP-E; KIF10; MCPH13; PPP1R61; centromere-associated protein E; Centromere autoantigen E (312kD); centromere protein E, 312kDa; kinesin family member 10; kinesin-7; kinesin-related protein CENPE; protein phosphatase 1, regulatory subunit 61

Abcam
mouse monoclonal (1H12)
  • immunocytochemistry; human; 1:200; loading ...; fig 2b
Abcam CENPE antibody (Abcam, ab5093) was used in immunocytochemistry on human samples at 1:200 (fig 2b). J Biol Chem (2018) ncbi
rabbit monoclonal (EPR4542(2))
  • western blot; human; 1:500; loading ...; fig 3b
Abcam CENPE antibody (Abcam, ab133583) was used in western blot on human samples at 1:500 (fig 3b). J Biol Chem (2018) ncbi
mouse monoclonal (1H12)
  • immunocytochemistry; human; 1:500; fig s4
In order to test if progerin elicits spatiotemporal deviations in mitotic processes in Hutchinson-Gilford progeria syndrome fibroblasts, Abcam CENPE antibody (Abcam, ab5093) was used in immunocytochemistry on human samples at 1:500 (fig s4). Oncotarget (2016) ncbi
mouse monoclonal (1H12)
  • immunocytochemistry; human; loading ...; fig s3a
  • western blot; human; loading ...; fig 1c
Abcam CENPE antibody (Abcam, ab5093) was used in immunocytochemistry on human samples (fig s3a) and in western blot on human samples (fig 1c). Oncotarget (2015) ncbi
mouse monoclonal (1H12)
  • immunocytochemistry; human
In order to investigate centrosome regulation during fever, Abcam CENPE antibody (Abcam, ab5093) was used in immunocytochemistry on human samples . Mol Biol Cell (2015) ncbi
mouse monoclonal (1H12)
  • immunohistochemistry; human; 1:1000; fig 5
In order to elucidate the contributions of Kid and CENP-E in chromosome congression, Abcam CENPE antibody (Abcam, ab5093) was used in immunohistochemistry on human samples at 1:1000 (fig 5). Nat Commun (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-5)
  • immunocytochemistry; human; loading ...; fig 6e
Santa Cruz Biotechnology CENPE antibody (Santa Cruz, sc-376685) was used in immunocytochemistry on human samples (fig 6e). Cell Cycle (2015) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; human; fig 2
Sigma-Aldrich CENPE antibody (Sigma, C7488) was used in western blot on human samples (fig 2). Sci Rep (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:1000; fig 1
  • western blot; human; 1:2000; fig 1
In order to elucidate the contributions of Kid and CENP-E in chromosome congression, Sigma-Aldrich CENPE antibody (Sigma, C7488) was used in immunohistochemistry on human samples at 1:1000 (fig 1) and in western blot on human samples at 1:2000 (fig 1). Nat Commun (2015) ncbi
Articles Reviewed
  1. Ciossani G, Overlack K, Petrovic A, Huis In t Veld P, Koerner C, Wohlgemuth S, et al. The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases. J Biol Chem. 2018;293:10084-10101 pubmed publisher
  2. Eisch V, Lu X, Gabriel D, Djabali K. Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts. Oncotarget. 2016;7:24700-18 pubmed publisher
  3. Zhang P, Zhang Y, Gao K, Wang Y, Jin X, Wei Y, et al. ASPP1/2-PP1 complexes are required for chromosome segregation and kinetochore-microtubule attachments. Oncotarget. 2015;6:41550-65 pubmed publisher
  4. Voets E, Marsman J, Demmers J, Beijersbergen R, Wolthuis R. The lethal response to Cdk1 inhibition depends on sister chromatid alignment errors generated by KIF4 and isoform 1 of PRC1. Sci Rep. 2015;5:14798 pubmed publisher
  5. Vertii A, Zimmerman W, Ivshina M, Doxsey S. Centrosome-intrinsic mechanisms modulate centrosome integrity during fever. Mol Biol Cell. 2015;26:3451-63 pubmed publisher
  6. Liu C, Chuang J, Sung C, Mao Y. A dynein independent role of Tctex-1 at the kinetochore. Cell Cycle. 2015;14:1379-88 pubmed publisher
  7. Iemura K, Tanaka K. Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules. Nat Commun. 2015;6:6447 pubmed publisher