This is a Validated Antibody Database (VAD) review about human CENPA, based on 28 published articles (read how Labome selects the articles), using CENPA antibody in all methods. It is aimed to help Labome visitors find the most suited CENPA antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CENPA synonym: CENP-A; CenH3; histone H3-like centromeric protein A; centromere autoantigen A; centromere protein A, 17kDa; centromere-specific histone

Abcam
mouse monoclonal (3-19)
  • immunocytochemistry; human; 1:300; loading ...; fig 3e
Abcam CENPA antibody (Abcam, ab13939) was used in immunocytochemistry on human samples at 1:300 (fig 3e). J Cell Sci (2018) ncbi
mouse monoclonal (3-19)
  • immunocytochemistry; human; 1:500; loading ...; fig 6e
Abcam CENPA antibody (Abcam, ab13939) was used in immunocytochemistry on human samples at 1:500 (fig 6e). Nat Commun (2016) ncbi
mouse monoclonal (3-19)
  • immunocytochemistry; human; 1:500
In order to describe how kinetochore fibers and microtubules are associated, Abcam CENPA antibody (Abcam, ab13939) was used in immunocytochemistry on human samples at 1:500. elife (2015) ncbi
mouse monoclonal (3-19)
  • immunocytochemistry; human; 1:400; fig 5
Abcam CENPA antibody (Abcam, ab13939) was used in immunocytochemistry on human samples at 1:400 (fig 5). J Cell Biol (2015) ncbi
mouse monoclonal (3-19)
  • chromatin immunoprecipitation; human; loading ...; fig 13
Abcam CENPA antibody (Abcam, ab13939) was used in chromatin immunoprecipitation on human samples (fig 13). Genome Biol (2015) ncbi
mouse monoclonal (3-19)
  • chromatin immunoprecipitation; human; fig 1
  • immunocytochemistry; human; fig 8
  • western blot; human; fig 1
Abcam CENPA antibody (Abcam, ab13939) was used in chromatin immunoprecipitation on human samples (fig 1), in immunocytochemistry on human samples (fig 8) and in western blot on human samples (fig 1). Epigenetics Chromatin (2015) ncbi
mouse monoclonal (3-19)
  • chromatin immunoprecipitation; human
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:200
Abcam CENPA antibody (Abcam, ab13939) was used in chromatin immunoprecipitation on human samples , in immunocytochemistry on human samples at 1:200 and in western blot on human samples at 1:200. Cell Div (2014) ncbi
mouse monoclonal (3-19)
  • immunocytochemistry; human; loading ...; fig 3c
Abcam CENPA antibody (Abcam, 3-19) was used in immunocytochemistry on human samples (fig 3c). J Cell Biol (2014) ncbi
mouse monoclonal (3-19)
  • immunocytochemistry; human; fig 2c
Abcam CENPA antibody (Abcam, ab13939) was used in immunocytochemistry on human samples (fig 2c). Nat Commun (2014) ncbi
mouse monoclonal (3-19)
  • immunocytochemistry; human; fig s10
Abcam CENPA antibody (Abcam, ab13939) was used in immunocytochemistry on human samples (fig s10). Mol Biol Cell (2014) ncbi
mouse monoclonal (3-19)
  • immunocytochemistry; human
Abcam CENPA antibody (Abcam, ab13939) was used in immunocytochemistry on human samples . Mol Cancer Ther (2014) ncbi
mouse monoclonal (3-19)
  • immunocytochemistry; human; 1:500
Abcam CENPA antibody (Abcam, ab13939) was used in immunocytochemistry on human samples at 1:500. PLoS ONE (2014) ncbi
mouse monoclonal (3-19)
  • immunohistochemistry - paraffin section; human; 1:100
  • western blot; human; 1:500
Abcam CENPA antibody (Abcam, ab13939) was used in immunohistochemistry - paraffin section on human samples at 1:100 and in western blot on human samples at 1:500. Pathol Res Pract (2014) ncbi
mouse monoclonal (3-19)
  • western blot; human
Abcam CENPA antibody (Abcam, ab13939) was used in western blot on human samples . PLoS ONE (2013) ncbi
mouse monoclonal (3-19)
  • immunohistochemistry - paraffin section; human
Abcam CENPA antibody (Abcam, ab13939) was used in immunohistochemistry - paraffin section on human samples . Cancer Res (2013) ncbi
Enzo Life Sciences
mouse monoclonal (3-19)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Enzo Life Sciences CENPA antibody (ENZO, 3-19) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (3-19)
  • immunocytochemistry; human; fig 1
Enzo Life Sciences CENPA antibody (Enzo, ADI-KAM-CC006) was used in immunocytochemistry on human samples (fig 1). J Cell Biol (2015) ncbi
Cell Signaling Technology
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 8d
In order to demonstrate that INPP5E regulates cell division, Cell Signaling Technology CENPA antibody (Cell Signaling, 2186S) was used in immunocytochemistry on human samples (fig 8d). Mol Cell Biol (2017) ncbi
rabbit polyclonal
  • proximity ligation assay; human; loading ...; fig s2f
  • immunocytochemistry; human; 1:400; loading ...; fig s2d
Cell Signaling Technology CENPA antibody (Cell Signaling, 2186) was used in proximity ligation assay on human samples (fig s2f) and in immunocytochemistry on human samples at 1:400 (fig s2d). Nat Immunol (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 4
  • immunocytochemistry; human; fig 5
Cell Signaling Technology CENPA antibody (Cell Signaling Technology, 2186) was used in chromatin immunoprecipitation on human samples (fig 4) and in immunocytochemistry on human samples (fig 5). Cell Rep (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:400; fig 9
Cell Signaling Technology CENPA antibody (Cell signaling, 2186) was used in immunocytochemistry on human samples at 1:400 (fig 9). elife (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 2
  • immunocytochemistry; Caenorhabditis elegans; fig 2
In order to examine how histone H3 threonine 118 alters chromosome structure, Cell Signaling Technology CENPA antibody (Cell Signalling Technology, 2186) was used in immunocytochemistry on human samples (fig 2) and in immunocytochemistry on Caenorhabditis elegans samples (fig 2). elife (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig s3
In order to elucidate the regulation and dynamics of H2A-T120 phosphorylation by Bub1, Cell Signaling Technology CENPA antibody (Cell Signaling, 2187) was used in immunocytochemistry on human samples at 1:100 (fig s3). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 2
In order to report that RSF1 localizes at mitotic kinetochores and directly binds PLK1, Cell Signaling Technology CENPA antibody (Cell signaling, 2186) was used in western blot on human samples at 1:1000 (fig 2). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:200
Cell Signaling Technology CENPA antibody (Cell Signaling Technologies, 2186) was used in immunocytochemistry on mouse samples at 1:200. Genome Res (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
In order to study the localization of CENP-A in the cell and at centromeres, Cell Signaling Technology CENPA antibody (Cell Signaling Technology, 2186) was used in western blot on human samples at 1:1000. elife (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
Cell Signaling Technology CENPA antibody (Cell Signaling Technologies, 2187) was used in western blot on human samples at 1:1000. J Biol Chem (2013) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
Cell Signaling Technology CENPA antibody (Cell Signaling Technologies, 2186) was used in western blot on human samples at 1:1000. J Biol Chem (2013) ncbi
EMD Millipore
rabbit monoclonal (NL41)
  • immunocytochemistry; human; 1:200; fig 1
EMD Millipore CENPA antibody (Millipore, 04-792) was used in immunocytochemistry on human samples at 1:200 (fig 1). Biol Open (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 1
  • immunocytochemistry; human; fig 8
  • western blot; human; fig 1
EMD Millipore CENPA antibody (Millipore, 07-574) was used in chromatin immunoprecipitation on human samples (fig 1), in immunocytochemistry on human samples (fig 8) and in western blot on human samples (fig 1). Epigenetics Chromatin (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:500
EMD Millipore CENPA antibody (Upstate, 07-574) was used in immunocytochemistry on human samples at 1:500. PLoS ONE (2014) ncbi
Articles Reviewed
  1. Soto M, García Santisteban I, Krenning L, Medema R, Raaijmakers J. Chromosomes trapped in micronuclei are liable to segregation errors. J Cell Sci. 2018;131: pubmed publisher
  2. Sierra Potchanant E, Cerabona D, Sater Z, He Y, Sun Z, Gehlhausen J, et al. INPP5E Preserves Genomic Stability through Regulation of Mitosis. Mol Cell Biol. 2017;37: pubmed publisher
  3. Despras E, Sittewelle M, Pouvelle C, Delrieu N, Cordonnier A, Kannouche P. Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA. Nat Commun. 2016;7:13326 pubmed publisher
  4. Cader M, Boroviak K, Zhang Q, Assadi G, Kempster S, Sewell G, et al. C13orf31 (FAMIN) is a central regulator of immunometabolic function. Nat Immunol. 2016;17:1046-56 pubmed publisher
  5. Huang C, Cheng J, Bawa Khalfe T, Yao X, Chin Y, Yeh E. SUMOylated ORC2 Recruits a Histone Demethylase to Regulate Centromeric Histone Modification and Genomic Stability. Cell Rep. 2016;15:147-157 pubmed publisher
  6. Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Llères D, et al. The cell proliferation antigen Ki-67 organises heterochromatin. elife. 2016;5:e13722 pubmed publisher
  7. Wike C, Graves H, Hawkins R, Gibson M, Ferdinand M, Zhang T, et al. Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis. elife. 2016;5:e11402 pubmed publisher
  8. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  9. O Connor A, Maffini S, Rainey M, Kaczmarczyk A, Gaboriau D, Musacchio A, et al. Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint. Biol Open. 2015;5:11-9 pubmed publisher
  10. Asghar A, Lajeunesse A, Dulla K, Combes G, Thebault P, Nigg E, et al. Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation. Nat Commun. 2015;6:8364 pubmed publisher
  11. Lee H, Park Y, Cho M, Chae S, Yoo Y, Kwon M, et al. The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores. Nat Commun. 2015;6:7904 pubmed publisher
  12. Nixon F, Gutiérrez Caballero C, Hood F, Booth D, Prior I, Royle S. The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. elife. 2015;4: pubmed publisher
  13. Prosser S, Sahota N, Pelletier L, Morrison C, Fry A. Nek5 promotes centrosome integrity in interphase and loss of centrosome cohesion in mitosis. J Cell Biol. 2015;209:339-48 pubmed publisher
  14. Zahn J, Kaplan M, Fischer S, Dai M, Meng F, Saha A, et al. Expansion of a novel endogenous retrovirus throughout the pericentromeres of modern humans. Genome Biol. 2015;16:74 pubmed publisher
  15. Athwal R, Walkiewicz M, Baek S, Fu S, Bui M, Camps J, et al. CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells. Epigenetics Chromatin. 2015;8:2 pubmed publisher
  16. González Barrios R, Soto Reyes E, Quiroz Baez R, Fabián Morales E, Díaz Chávez J, Del Castillo V, et al. Differential distribution of HP1 proteins after trichostatin a treatment influences chromosomal stability in HCT116 and WI-38 cells. Cell Div. 2014;9:6 pubmed publisher
  17. Logsdon G, Barrey E, Bassett E, Denizio J, Guo L, Panchenko T, et al. Both tails and the centromere targeting domain of CENP-A are required for centromere establishment. J Cell Biol. 2015;208:521-31 pubmed publisher
  18. Espert A, Uluocak P, Bastos R, Mangat D, Graab P, Gruneberg U. PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing. J Cell Biol. 2014;206:833-42 pubmed publisher
  19. Shandilya J, Toska E, Richard D, Medler K, Roberts S. WT1 interacts with MAD2 and regulates mitotic checkpoint function. Nat Commun. 2014;5:4903 pubmed publisher
  20. Smith C, Matheson T, Trombly D, Sun X, Campeau E, Han X, et al. A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli. Mol Biol Cell. 2014;25:2866-81 pubmed publisher
  21. Ivanauskiene K, Delbarre E, McGhie J, Küntziger T, Wong L, Collas P. The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin and is important for telomere integrity. Genome Res. 2014;24:1584-94 pubmed publisher
  22. Bodor D, Mata J, Sergeev M, David A, Salimian K, Panchenko T, et al. The quantitative architecture of centromeric chromatin. elife. 2014;3:e02137 pubmed publisher
  23. Mäki Jouppila J, Laine L, Rehnberg J, Narvi E, Tiikkainen P, Hukasova E, et al. Centmitor-1, a novel acridinyl-acetohydrazide, possesses similar molecular interaction field and antimitotic cellular phenotype as rigosertib, on 01910.Na. Mol Cancer Ther. 2014;13:1054-66 pubmed publisher
  24. Stimpson K, Sullivan L, Kuo M, Sullivan B. Nucleolar organization, ribosomal DNA array stability, and acrocentric chromosome integrity are linked to telomere function. PLoS ONE. 2014;9:e92432 pubmed publisher
  25. Gu X, Fu J, Feng X, Huang X, Wang S, Chen X, et al. Expression and prognostic relevance of centromere protein A in primary osteosarcoma. Pathol Res Pract. 2014;210:228-33 pubmed publisher
  26. Hofmann J, Tegha Dunghu J, Dräger S, Will C, Luhrmann R, Gruss O. The Prp19 complex directly functions in mitotic spindle assembly. PLoS ONE. 2013;8:e74851 pubmed publisher
  27. Tan E, Caro S, Potnis A, Lanza C, Slawson C. O-linked N-acetylglucosamine cycling regulates mitotic spindle organization. J Biol Chem. 2013;288:27085-99 pubmed publisher
  28. Orecchioni S, Gregato G, Martin Padura I, Reggiani F, Braidotti P, Mancuso P, et al. Complementary populations of human adipose CD34+ progenitor cells promote growth, angiogenesis, and metastasis of breast cancer. Cancer Res. 2013;73:5880-91 pubmed publisher