This is a Validated Antibody Database (VAD) review about human CDKL5, based on 5 published articles (read how Labome selects the articles), using CDKL5 antibody in all methods. It is aimed to help Labome visitors find the most suited CDKL5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CDKL5 synonym: CFAP247; EIEE2; ISSX; STK9; cyclin-dependent kinase-like 5; cyclin dependent kinase 5 transcript; serine/threonine kinase 9; serine/threonine-protein kinase 9

Knockout validation
Abcam
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 2a
Abcam CDKL5 antibody (Abcam, ab22453) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2a). EMBO J (2018) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 2a
Sigma-Aldrich CDKL5 antibody (Sigma, HPA002847) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2a). EMBO J (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-12)
  • western blot; human; loading ...; fig 2a
Santa Cruz Biotechnology CDKL5 antibody (SantaCruz, sc-376314) was used in western blot on human samples (fig 2a). Hum Mol Genet (2017) ncbi
Abcam
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 2a
Abcam CDKL5 antibody (Abcam, ab22453) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2a). EMBO J (2018) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 2a
Sigma-Aldrich CDKL5 antibody (Sigma, HPA002847) was used in western blot knockout validation on mouse samples at 1:1000 (fig 2a). EMBO J (2018) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; fig 2
  • western blot; mouse; fig 3
In order to characterize the interaction between Shootin1 and CDKL5 and how they work together to regulate neuronal polarization, Sigma-Aldrich CDKL5 antibody (Sigma-Aldrich, HPA002847) was used in immunocytochemistry on mouse samples (fig 2) and in western blot on mouse samples (fig 3). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse
  • western blot; mouse
Sigma-Aldrich CDKL5 antibody (Sigma, -) was used in immunohistochemistry on mouse samples and in western blot on mouse samples . PLoS ONE (2014) ncbi
Neuromab
mouse monoclonal (EA7)
  • western blot; mouse; loading ...; fig 1a
Neuromab CDKL5 antibody (neuromab, EA7) was used in western blot on mouse samples (fig 1a). Neurosci Lett (2017) ncbi
Articles Reviewed
  1. Baltussen L, Negraes P, Silvestre M, Claxton S, Moeskops M, Christodoulou E, et al. Chemical genetic identification of CDKL5 substrates reveals its role in neuronal microtubule dynamics. EMBO J. 2018;37: pubmed publisher
  2. Barbiero I, Peroni D, Tramarin M, Chandola C, Rusconi L, Landsberger N, et al. The neurosteroid pregnenolone reverts microtubule derangement induced by the loss of a functional CDKL5-IQGAP1 complex. Hum Mol Genet. 2017;26:3520-3530 pubmed publisher
  3. Enga R, Rice A, Weller P, Subler M, Lee D, Hall C, et al. Initial characterization of behavior and ketamine response in a mouse knockout of the post-synaptic effector gene Anks1b. Neurosci Lett. 2017;641:26-32 pubmed publisher
  4. Nawaz M, Giarda E, Bedogni F, La Montanara P, Ricciardi S, Ciceri D, et al. CDKL5 and Shootin1 Interact and Concur in Regulating Neuronal Polarization. PLoS ONE. 2016;11:e0148634 pubmed publisher
  5. Amendola E, Zhan Y, Mattucci C, Castroflorio E, Calcagno E, Fuchs C, et al. Mapping pathological phenotypes in a mouse model of CDKL5 disorder. PLoS ONE. 2014;9:e91613 pubmed publisher