This is a Validated Antibody Database (VAD) review about human CD74, based on 17 published articles (read how Labome selects the articles), using CD74 antibody in all methods. It is aimed to help Labome visitors find the most suited CD74 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CD74 synonym: DHLAG; HLADG; II; Ia-GAMMA; p33

Santa Cruz Biotechnology
mouse monoclonal (CerCLIP.1)
  • immunoprecipitation; human; loading ...; fig 2c
  • western blot; human; 1:3000; loading ...; fig 2a
In order to study the antigen presentation by HLA-DP84Gly through the class I antigen processing pathway, Santa Cruz Biotechnology CD74 antibody (Santa Cruz Biotechnology, sc-12725) was used in immunoprecipitation on human samples (fig 2c) and in western blot on human samples at 1:3000 (fig 2a). Nat Commun (2017) ncbi
mouse monoclonal (PIN.1)
  • western blot; human; 1:500; loading ...; fig 2b
In order to study the antigen presentation by HLA-DP84Gly through the class I antigen processing pathway, Santa Cruz Biotechnology CD74 antibody (Santa Cruz Biotechnology, sc-47742) was used in western blot on human samples at 1:500 (fig 2b). Nat Commun (2017) ncbi
mouse monoclonal (LN-2)
  • immunocytochemistry; human; 1:200; loading ...; fig 3h
  • western blot; human; 1:1000; loading ...; fig 2b
In order to study the antigen presentation by HLA-DP84Gly through the class I antigen processing pathway, Santa Cruz Biotechnology CD74 antibody (Santa Cruz Biotechnology, sc-6262) was used in immunocytochemistry on human samples at 1:200 (fig 3h) and in western blot on human samples at 1:1000 (fig 2b). Nat Commun (2017) ncbi
mouse monoclonal (LN-2)
  • blocking or activating experiments; human
  • flow cytometry; human; fig 2b
In order to explore how the CD74/CD44 MIF receptor contributes to rheumatoid arthritis, Santa Cruz Biotechnology CD74 antibody (Santa Cruz, LN2) was used in blocking or activating experiments on human samples and in flow cytometry on human samples (fig 2b). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal
  • flow cytometry; human; fig 2b
In order to explore how the CD74/CD44 MIF receptor contributes to rheumatoid arthritis, Santa Cruz Biotechnology CD74 antibody (Santa Cruz, LN2) was used in flow cytometry on human samples (fig 2b). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (CerCLIP.1)
  • flow cytometry; human; loading ...; fig 1a
In order to study Type 1 diabetes, HLA-DQ2 and DQ8 molecules, and HLA-DM effect on invariant chain-derived CLIP peptides, Santa Cruz Biotechnology CD74 antibody (SantaCruz, Cer.CLIP1) was used in flow cytometry on human samples (fig 1a). Eur J Immunol (2016) ncbi
mouse monoclonal (LN-2)
  • ELISA; human; fig 2
Santa Cruz Biotechnology CD74 antibody (Santa Cruz, sc-6262) was used in ELISA on human samples (fig 2). Antioxid Redox Signal (2015) ncbi
mouse monoclonal (D-6)
  • flow cytometry; mouse
Santa Cruz Biotechnology CD74 antibody (Santa Cruz, sc-137217) was used in flow cytometry on mouse samples . Monoclon Antib Immunodiagn Immunother (2014) ncbi
Invitrogen
mouse monoclonal (5-329)
  • flow cytometry; human; loading ...; fig 2d
Invitrogen CD74 antibody (eBioscience, 11-0748-41) was used in flow cytometry on human samples (fig 2d). Cell (2018) ncbi
mouse monoclonal (5-329)
  • other; human; loading ...; fig st1
  • flow cytometry; human; loading ...; fig st3
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Invitrogen CD74 antibody (eBioscience, 5-329) was used in other on human samples (fig st1) and in flow cytometry on human samples (fig st3). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (5-329)
  • flow cytometry; human; 1:200; fig st3
Invitrogen CD74 antibody (eBioscience, 12-0748-42) was used in flow cytometry on human samples at 1:200 (fig st3). Leukemia (2016) ncbi
mouse monoclonal (5-329)
  • flow cytometry; human; fig 3
Invitrogen CD74 antibody (eBioscience, 5-329) was used in flow cytometry on human samples (fig 3). Nephrol Dial Transplant (2015) ncbi
mouse monoclonal (LN2)
  • immunohistochemistry - paraffin section; human; tbl 1
In order to discuss factors that influence heat-induced antigen retrieval, Invitrogen CD74 antibody (Zymed, LN2) was used in immunohistochemistry - paraffin section on human samples (tbl 1). Pathology (2010) ncbi
BioLegend
mouse monoclonal (LN2)
  • flow cytometry; human; loading ...; fig 4f
BioLegend CD74 antibody (Biologend, 326807) was used in flow cytometry on human samples (fig 4f). Nat Immunol (2018) ncbi
mouse monoclonal (LN2)
BioLegend CD74 antibody (Biolegend, LN2) was used . PLoS ONE (2014) ncbi
Abcam
mouse monoclonal (LN2)
  • western blot; human; 1:1000; loading ...; fig 11e
Abcam CD74 antibody (Abcam, ab9514) was used in western blot on human samples at 1:1000 (fig 11e). J Biol Chem (2018) ncbi
Enzo Life Sciences
mouse monoclonal (PIN.1)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Enzo Life Sciences CD74 antibody (ENZO, PIN.1) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
Bio-Rad
mouse monoclonal (Bu45)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Bio-Rad CD74 antibody (SEROTEC, BU45) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
MilliporeSigma
mouse monoclonal (1D1)
  • immunohistochemistry - paraffin section; human; loading ...; fig 1a
  • immunocytochemistry; human; loading ...; fig s2a
  • western blot; human; loading ...; fig 2f
MilliporeSigma CD74 antibody (Sigma, SAB1402715) was used in immunohistochemistry - paraffin section on human samples (fig 1a), in immunocytochemistry on human samples (fig s2a) and in western blot on human samples (fig 2f). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; fig 7
MilliporeSigma CD74 antibody (Sigma, HPA010592) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 7). Oncotarget (2016) ncbi
BD Biosciences
mouse monoclonal (M-B741)
  • flow cytometry; human; 1:500; loading ...; fig 1a
In order to study the antigen presentation by HLA-DP84Gly through the class I antigen processing pathway, BD Biosciences CD74 antibody (BD Biosciences, 555540) was used in flow cytometry on human samples at 1:500 (fig 1a). Nat Commun (2017) ncbi
mouse monoclonal (M-B741)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences CD74 antibody (BD, 555540) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (M-B741)
  • flow cytometry; mouse
BD Biosciences CD74 antibody (BD, M-B741) was used in flow cytometry on mouse samples . PLoS ONE (2014) ncbi
Articles Reviewed
  1. De R, Sarkar S, Mazumder S, Debsharma S, Siddiqui A, Saha S, et al. Macrophage migration inhibitory factor regulates mitochondrial dynamics and cell growth of human cancer cell lines through CD74-NF-κB signaling. J Biol Chem. 2018;293:19740-19760 pubmed publisher
  2. Kinchen J, Chen H, Parikh K, Antanaviciute A, Jagielowicz M, Fawkner Corbett D, et al. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell. 2018;175:372-386.e17 pubmed publisher
  3. Kong X, Martinez Barricarte R, Kennedy J, Mele F, Lazarov T, Deenick E, et al. Disruption of an antimycobacterial circuit between dendritic and helper T cells in human SPPL2a deficiency. Nat Immunol. 2018;19:973-985 pubmed publisher
  4. Yamashita Y, Anczurowski M, Nakatsugawa M, Tanaka M, Kagoya Y, Sinha A, et al. HLA-DP84Gly constitutively presents endogenous peptides generated by the class I antigen processing pathway. Nat Commun. 2017;8:15244 pubmed publisher
  5. Yoo S, Leng L, Kim B, Du X, Tilstam P, Kim K, et al. MIF allele-dependent regulation of the MIF coreceptor CD44 and role in rheumatoid arthritis. Proc Natl Acad Sci U S A. 2016;113:E7917-E7926 pubmed
  6. Liu Z, Chu S, Yao S, Li Y, Fan S, Sun X, et al. CD74 interacts with CD44 and enhances tumorigenesis and metastasis via RHOA-mediated cofilin phosphorylation in human breast cancer cells. Oncotarget. 2016;7:68303-68313 pubmed publisher
  7. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  8. D Amato Brito C, Cipriano D, Colin D, Germain S, Seimbille Y, Robert J, et al. Role of MIF/CD74 signaling pathway in the development of pleural mesothelioma. Oncotarget. 2016;7:11512-25 pubmed publisher
  9. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  10. Zhou Z, Reyes Vargas E, Escobar H, Rudd B, Rockwood A, Delgado J, et al. Type 1 diabetes associated HLA-DQ2 and DQ8 molecules are relatively resistant to HLA-DM mediated release of invariant chain-derived CLIP peptides. Eur J Immunol. 2016;46:834-45 pubmed publisher
  11. Brown P, Wong K, Felce S, Lyne L, Spearman H, Soilleux E, et al. FOXP1 suppresses immune response signatures and MHC class II expression in activated B-cell-like diffuse large B-cell lymphomas. Leukemia. 2016;30:605-16 pubmed publisher
  12. Stoppe C, Rex S, Goetzenich A, Kraemer S, Emontzpohl C, Soppert J, et al. Interaction of MIF Family Proteins in Myocardial Ischemia/Reperfusion Damage and Their Influence on Clinical Outcome of Cardiac Surgery Patients. Antioxid Redox Signal. 2015;23:865-79 pubmed publisher
  13. Ikeda T, Hirata S, Takamatsu K, Haruta M, Tsukamoto H, Ito T, et al. Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells. PLoS ONE. 2014;9:e115198 pubmed publisher
  14. Rogacev K, Zawada A, Hundsdorfer J, Achenbach M, Held G, Fliser D, et al. Immunosuppression and monocyte subsets. Nephrol Dial Transplant. 2015;30:143-53 pubmed publisher
  15. Genève L, Gauthier C, Thibodeau J. The D-6 mouse monoclonal antibody recognizes the CD74 cytoplasmic tail. Monoclon Antib Immunodiagn Immunother. 2014;33:221-7 pubmed publisher
  16. Morris K, Nofchissey R, Pinchuk I, Beswick E. Chronic macrophage migration inhibitory factor exposure induces mesenchymal epithelial transition and promotes gastric and colon cancers. PLoS ONE. 2014;9:e98656 pubmed publisher
  17. Leong A, Haffajee Z. Citraconic anhydride: a new antigen retrieval solution. Pathology. 2010;42:77-81 pubmed publisher