This is a Validated Antibody Database (VAD) review about human CD59, based on 41 published articles (read how Labome selects the articles), using CD59 antibody in all methods. It is aimed to help Labome visitors find the most suited CD59 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CD59 synonym: 16.3A5; 1F5; EJ16; EJ30; EL32; G344; HRF-20; HRF20; MAC-IP; MACIF; MEM43; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20

Invitrogen
domestic rabbit polyclonal
  • western blot; mouse; 1:500; fig 5k
Invitrogen CD59 antibody (Invitrogen, PA5-97565) was used in western blot on mouse samples at 1:500 (fig 5k). Front Cell Dev Biol (2021) ncbi
mouse monoclonal (OV9A2)
  • flow cytometry; human; fig 6a
In order to investigate the protective effect of single domain camelid antibodies against botulinum neurotoxin, Invitrogen CD59 antibody (Miltenyi Biotec, 17-0596-42) was used in flow cytometry on human samples (fig 6a). Nat Commun (2017) ncbi
mouse monoclonal (MEM-43)
  • western blot; human; loading ...; fig s2
In order to discuss methods to increase the urinary extracellular vesicle yield, Invitrogen CD59 antibody (Thermo Scientific, MEM-43) was used in western blot on human samples (fig s2). Eur J Pharm Sci (2017) ncbi
mouse monoclonal (MEM-43)
  • immunocytochemistry; pigs ; 1:100; loading ...; fig 1b
  • western blot; pigs ; 1:500; loading ...; fig s1f
In order to study factors that limit membrane attack complexes in models of macular degeneration, Invitrogen CD59 antibody (Thermo Scientific, MA1-19133) was used in immunocytochemistry on pigs samples at 1:100 (fig 1b) and in western blot on pigs samples at 1:500 (fig s1f). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (MEM-43)
  • immunocytochemistry; human; fig 3
In order to study the essential alternative complement pathway components regulated by TNF and how they impair activation of protein c in human glomerular endothelial cells, Invitrogen CD59 antibody (Thermo Fisher, MA1-82206) was used in immunocytochemistry on human samples (fig 3). J Immunol (2016) ncbi
mouse monoclonal (MEM-43)
  • immunoprecipitation; human; loading ...; fig 3a
In order to study mechanisms of programed cell death of red blood cells, Invitrogen CD59 antibody (ThermoScientific, MEM-43) was used in immunoprecipitation on human samples (fig 3a). Cell Death Dis (2015) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human; fig s4
  • immunocytochemistry; human; 1:20
In order to discuss methods to efficiently generate induced pluripotent stem cells from human adult blood mononuclear cells, Invitrogen CD59 antibody (Life Technologies, MHCD5904) was used in flow cytometry on human samples (fig s4) and in immunocytochemistry on human samples at 1:20. Stem Cells Transl Med (2015) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human
In order to discuss the features of paroxysmal nocturnal hemoglobinuria, Invitrogen CD59 antibody (Invitrogen, MEM-43) was used in flow cytometry on human samples . Cytometry B Clin Cytom (2015) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human
Invitrogen CD59 antibody (Invitrogen, MEM-43) was used in flow cytometry on human samples . Hematology (2015) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human
In order to evaluate somatic mutation rate using a method to measure the proportion of PIG-A mutant peripheral blood granulocytes, Invitrogen CD59 antibody (noco, MHCD5904) was used in flow cytometry on human samples . PLoS ONE (2013) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human; fig 3
In order to investigate the molecular basis of chronic Coombs-negative hemolysis and relapsing polyneuropathy presenting as chronic inflammatory demyelinating polyradiculoneuropathy in North-African Jewish infants, Invitrogen CD59 antibody (noco, MHCD5904) was used in flow cytometry on human samples (fig 3). Blood (2013) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; rhesus macaque; fig 1
In order to use cross reactive antibodies to identify rhesus macaque erythrocytes, Invitrogen CD59 antibody (Invitrogen, MEM-43) was used in flow cytometry on rhesus macaque samples (fig 1). Cytometry A (2012) ncbi
mouse monoclonal (MEM-43)
  • immunocytochemistry; human
In order to discuss the use of umbilical cord blood as a source of transplantable hematopoietic stem cells, Invitrogen CD59 antibody (Invitrogen, MEM-43) was used in immunocytochemistry on human samples . Transfusion (2011) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human; fig 2
In order to discuss ways to standardize flow cytometry methods to diagnose paroxysmal nocturnal hemoglobinuria, Invitrogen CD59 antibody (Invitrogen, MEM43) was used in flow cytometry on human samples (fig 2). Cytometry B Clin Cytom (2010) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human; 1:40
In order to characterize CHO A L mutant expression kinetics after treatment with EMS, asbestos, and gamma radiation, Invitrogen CD59 antibody (Caltag, noca) was used in flow cytometry on human samples at 1:40. Cytometry A (2009) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human
In order to examine how FCGR3B copy number affects immune responses, Invitrogen CD59 antibody (Invitrogen, Mem-43) was used in flow cytometry on human samples . J Exp Med (2008) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human
In order to examine loss of CD20 in chronic lymphocytic leukemia, Invitrogen CD59 antibody (Caltag, MEM-43) was used in flow cytometry on human samples . J Immunol (2006) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human
In order to use flow cytometry to characterize 123 cases of B-cell chronic lymphocytic leukemia, Invitrogen CD59 antibody (Caltag, MEM-43) was used in flow cytometry on human samples . J Cell Physiol (2005) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human
In order to use flow cytometry to characterize cells from patients with paroxysmal nocturnal hemoglobinuria, Invitrogen CD59 antibody (Caltag, MEM-43) was used in flow cytometry on human samples . Am J Clin Pathol (2004) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human
In order to test if human fetal liver cells expressing CD34 and CK7/8 represent a common stem cell for both the hematopoietic and hepatic systems, Invitrogen CD59 antibody (Caltag, MEM43) was used in flow cytometry on human samples . J Hepatol (2004) ncbi
mouse monoclonal (MEM-43)
  • immunohistochemistry; human
In order to discuss the use of Rituximab, Invitrogen CD59 antibody (Caltag, MEM-43) was used in immunohistochemistry on human samples . J Am Acad Dermatol (2003) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human
In order to investigate the cellular processing of PrP (C) using platelets, Invitrogen CD59 antibody (Caltag, MEM-43) was used in flow cytometry on human samples . Br J Haematol (1998) ncbi
mouse monoclonal (MEM-43)
  • immunoprecipitation; human; fig 1
In order to report that GPI-anchored proteins associate with G proteins, Invitrogen CD59 antibody (Caltag, MEM-43) was used in immunoprecipitation on human samples (fig 1). Proc Natl Acad Sci U S A (1996) ncbi
mouse monoclonal (MEM-43)
  • western blot; human; fig 4
In order to study CD55 and CD59 in Schwann cells, Invitrogen CD59 antibody (Caltag Laboratories, clone MEM-43) was used in western blot on human samples (fig 4). J Neurochem (1996) ncbi
mouse monoclonal (MEM-43)
  • immunoprecipitation; human
In order to determine the lymphoid lineage involvement in paroxysmal nocturnal hemoglobinuria, Invitrogen CD59 antibody (caltag, MEM-43) was used in immunoprecipitation on human samples . Am J Hematol (1995) ncbi
mouse monoclonal (MEM-43)
  • flow cytometry; human
In order to study expression of CD59, Invitrogen CD59 antibody (Zymed, MEM-43) was used in flow cytometry on human samples . Eur J Immunol (1990) ncbi
BioLegend
mouse monoclonal (p282 (H19))
  • flow cytometry; pigs ; 1:50; loading ...; fig 4a
BioLegend CD59 antibody (BioLegend, 304708) was used in flow cytometry on pigs samples at 1:50 (fig 4a). Cells (2021) ncbi
mouse monoclonal (p282 (H19))
  • immunohistochemistry; human; loading ...; fig 10c
BioLegend CD59 antibody (Biolegend, p282) was used in immunohistochemistry on human samples (fig 10c). Bone Rep (2020) ncbi
mouse monoclonal (p282 (H19))
  • blocking or activating experiments; mouse; fig s5i
BioLegend CD59 antibody (Biolegend, p282/H19) was used in blocking or activating experiments on mouse samples (fig s5i). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (p282 (H19))
  • flow cytometry; human
BioLegend CD59 antibody (Biolegend, #304706) was used in flow cytometry on human samples . Am J Transplant (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-7)
  • western blot; mouse; loading ...; fig 2i
Santa Cruz Biotechnology CD59 antibody (Santa Cruz Biotechnology Inc, sc-133170) was used in western blot on mouse samples (fig 2i). Cancer Res (2018) ncbi
mouse monoclonal (B-3)
  • western blot; human; loading ...; fig 3
Santa Cruz Biotechnology CD59 antibody (Santa Cruz, sc-133171) was used in western blot on human samples (fig 3). J Proteome Res (2017) ncbi
mouse monoclonal (B-3)
  • immunohistochemistry - paraffin section; human
Santa Cruz Biotechnology CD59 antibody (Santa Cruz Biotechnology, sc-133171) was used in immunohistochemistry - paraffin section on human samples . Arch Pathol Lab Med (2014) ncbi
rat monoclonal (YTH53.1)
  • western blot; human; fig 7b
Santa Cruz Biotechnology CD59 antibody (Santa Cruz Biotechnology, YTH53.1) was used in western blot on human samples (fig 7b). Am J Physiol Gastrointest Liver Physiol (2014) ncbi
Abcam
domestic rabbit monoclonal (EPR6425(2))
  • immunohistochemistry - paraffin section; human; fig s1
Abcam CD59 antibody (abcam, ab133707) was used in immunohistochemistry - paraffin section on human samples (fig s1). Sci Rep (2016) ncbi
Abnova
mouse monoclonal (MEM-43/5)
  • immunocytochemistry; human; 1:50; loading ...; fig 1a
Abnova CD59 antibody (ABNova, MEM-43/5) was used in immunocytochemistry on human samples at 1:50 (fig 1a). MBio (2018) ncbi
Bio-Rad
mouse monoclonal (MEM-43)
  • immunocytochemistry; human
In order to investigate the effect of TNF and IL-10 on synovial fibroblasts, Bio-Rad CD59 antibody (AbD Serotec, MCA1054GA) was used in immunocytochemistry on human samples . Cell Tissue Res (2014) ncbi
BD Biosciences
mouse monoclonal (p282)
  • other; human; 500 ug/ml; fig 1
BD Biosciences CD59 antibody (Becton Dickinson, 555761) was used in other on human samples at 500 ug/ml (fig 1). J Extracell Vesicles (2016) ncbi
mouse monoclonal (p282)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences CD59 antibody (BD, 555764) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (p282)
  • flow cytometry; human; fig S5
In order to use a new model which initiates alternate pathway activation on retinal pigment epithelial cells to investigate cell death, BD Biosciences CD59 antibody (BD Biosciences, clone p282) was used in flow cytometry on human samples (fig S5). Invest Ophthalmol Vis Sci (2014) ncbi
mouse monoclonal (p282)
  • flow cytometry; human
BD Biosciences CD59 antibody (BD Pharmingen, p282/H19) was used in flow cytometry on human samples . J Biol Chem (2014) ncbi
Articles Reviewed
  1. Tritschler H, Fischer K, Seissler J, Fiedler J, Halbgebauer R, Huber Lang M, et al. New Insights into Xenotransplantation for Cartilage Repair: Porcine Multi-Genetically Modified Chondrocytes as a Promising Cell Source. Cells. 2021;10: pubmed publisher
  2. Siems S, Jahn O, Hoodless L, Jung R, Hesse D, Mobius W, et al. Proteome Profile of Myelin in the Zebrafish Brain. Front Cell Dev Biol. 2021;9:640169 pubmed publisher
  3. Zhang Z, Le K, La Placa D, Armstrong B, Miller M, Shively J. CXCR2 specific endocytosis of immunomodulatory peptide LL-37 in human monocytes and formation of LL-37 positive large vesicles in differentiated monoosteophils. Bone Rep. 2020;12:100237 pubmed publisher
  4. Zeltzer S, Zeltzer C, Igarashi S, Wilson J, Donaldson J, Goodrum F. Virus Control of Trafficking from Sorting Endosomes. MBio. 2018;9: pubmed publisher
  5. Wan Y, Wang L, Zhu C, Zheng Q, Wang G, Tong J, et al. Aptamer-Conjugated Extracellular Nanovesicles for Targeted Drug Delivery. Cancer Res. 2018;78:798-808 pubmed publisher
  6. Huang N, Pishesha N, Mukherjee J, Zhang S, Deshycka R, Sudaryo V, et al. Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin. Nat Commun. 2017;8:423 pubmed publisher
  7. Staubach S, Müller S, Pekmez M, Hanisch F. Classical Galactosemia: Insight into Molecular Pathomechanisms by Differential Membrane Proteomics of Fibroblasts under Galactose Stress. J Proteome Res. 2017;16:516-527 pubmed publisher
  8. Puhka M, Nordberg M, Valkonen S, Rannikko A, Kallioniemi O, Siljander P, et al. KeepEX, a simple dilution protocol for improving extracellular vesicle yields from urine. Eur J Pharm Sci. 2017;98:30-39 pubmed publisher
  9. Tan L, Toops K, Lakkaraju A. Protective responses to sublytic complement in the retinal pigment epithelium. Proc Natl Acad Sci U S A. 2016;113:8789-94 pubmed publisher
  10. Clark A, Petty H. Identification of lesion subtypes in biopsies of ductal carcinoma in situ of the breast using biomarker ratio imaging microscopy. Sci Rep. 2016;6:27039 pubmed publisher
  11. Belov L, Matic K, Hallal S, Best O, Mulligan S, Christopherson R. Extensive surface protein profiles of extracellular vesicles from cancer cells may provide diagnostic signatures from blood samples. J Extracell Vesicles. 2016;5:25355 pubmed publisher
  12. Krampitz G, George B, Willingham S, Volkmer J, Weiskopf K, Jahchan N, et al. Identification of tumorigenic cells and therapeutic targets in pancreatic neuroendocrine tumors. Proc Natl Acad Sci U S A. 2016;113:4464-9 pubmed publisher
  13. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  14. Sartain S, Turner N, Moake J. TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human Glomerular Endothelial Cells. J Immunol. 2016;196:832-45 pubmed publisher
  15. LaRocca T, Stivison E, Mal Sarkar T, Hooven T, Hod E, Spitalnik S, et al. CD59 signaling and membrane pores drive Syk-dependent erythrocyte necroptosis. Cell Death Dis. 2015;6:e1773 pubmed publisher
  16. Thomas K, Valenzuela N, Gjertson D, Mulder A, Fishbein M, Parry G, et al. An Anti-C1s Monoclonal, TNT003, Inhibits Complement Activation Induced by Antibodies Against HLA. Am J Transplant. 2015;15:2037-49 pubmed publisher
  17. Chou B, Gu H, Gao Y, Dowey S, Wang Y, Shi J, et al. A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach. Stem Cells Transl Med. 2015;4:320-32 pubmed publisher
  18. Liew M, Farley M, Andreasen J, Parker C, Wittwer C. Rare event counting of CD59- red cells in human blood: A 47-month experience using PNH consensus guidelines for WBC and RBC testing in a reference lab. Cytometry B Clin Cytom. 2015;88:261-9 pubmed publisher
  19. Shang Y, Chai N, Gu Y, Ding L, Yang Y, Zhou J, et al. Systematic immunohistochemical analysis of the expression of CD46, CD55, and CD59 in colon cancer. Arch Pathol Lab Med. 2014;138:910-9 pubmed publisher
  20. Mrosewski I, Jork N, Gorte K, Conrad C, Wiegand E, Kohl B, et al. Regulation of osteoarthritis-associated key mediators by TNF? and IL-10: effects of IL-10 overexpression in human synovial fibroblasts and a synovial cell line. Cell Tissue Res. 2014;357:207-23 pubmed publisher
  21. Wu Y, Wang Y, Qin F, Wang Z, Wang Y, Yang Y, et al. CD55 limits sensitivity to complement-dependent cytolysis triggered by heterologous expression of ?-gal xenoantigen in colon tumor cells. Am J Physiol Gastrointest Liver Physiol. 2014;306:G1056-64 pubmed publisher
  22. Yang P, Baciu P, Kerrigan B, Etheridge M, Sung E, Toimil B, et al. Retinal pigment epithelial cell death by the alternative complement cascade: role of membrane regulatory proteins, calcium, PKC, and oxidative stress. Invest Ophthalmol Vis Sci. 2014;55:3012-21 pubmed publisher
  23. Sipol A, Babenko E, Borisov V, Naumova E, Boyakova E, Yakunin D, et al. An inter-laboratory comparison of PNH clone detection by high-sensitivity flow cytometry in a Russian cohort. Hematology. 2015;20:31-8 pubmed publisher
  24. Du Y, Teng X, Wang N, Zhang X, Chen J, Ding P, et al. NF-?B and enhancer-binding CREB protein scaffolded by CREB-binding protein (CBP)/p300 proteins regulate CD59 protein expression to protect cells from complement attack. J Biol Chem. 2014;289:2711-24 pubmed publisher
  25. Rondelli T, Berardi M, Peruzzi B, Boni L, Caporale R, Dolara P, et al. The frequency of granulocytes with spontaneous somatic mutations: a wide distribution in a normal human population. PLoS ONE. 2013;8:e54046 pubmed publisher
  26. Nevo Y, Ben Zeev B, Tabib A, Straussberg R, Anikster Y, Shorer Z, et al. CD59 deficiency is associated with chronic hemolysis and childhood relapsing immune-mediated polyneuropathy. Blood. 2013;121:129-35 pubmed publisher
  27. Byrnes C, Terry Lee Y, Donahue R, Miller J. Identification of a cross-reacting, monoclonal anti-human CD233 antibody for identification and sorting of rhesus macaque erythrocytes. Cytometry A. 2012;81:165-8 pubmed publisher
  28. Barcena A, Muench M, Kapidzic M, Gormley M, Goldfien G, Fisher S. Human placenta and chorion: potential additional sources of hematopoietic stem cells for transplantation. Transfusion. 2011;51 Suppl 4:94S-105S pubmed publisher
  29. Borowitz M, Craig F, DiGiuseppe J, Illingworth A, Rosse W, Sutherland D, et al. Guidelines for the diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria and related disorders by flow cytometry. Cytometry B Clin Cytom. 2010;78:211-30 pubmed publisher
  30. Keysar S, Fox M. Kinetics of CHO A L mutant expression after treatment with gamma radiation, EMS, and asbestos. Cytometry A. 2009;75:412-9 pubmed publisher
  31. Willcocks L, Lyons P, Clatworthy M, Robinson J, Yang W, Newland S, et al. Copy number of FCGR3B, which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake. J Exp Med. 2008;205:1573-82 pubmed publisher
  32. Williams M, Densmore J, Pawluczkowycz A, Beum P, Kennedy A, Lindorfer M, et al. Thrice-weekly low-dose rituximab decreases CD20 loss via shaving and promotes enhanced targeting in chronic lymphocytic leukemia. J Immunol. 2006;177:7435-43 pubmed
  33. Zucchetto A, Sonego P, Degan M, Bomben R, Dal Bo M, Russo S, et al. Signature of B-CLL with different prognosis by Shrunken centroids of surface antigen expression profiling. J Cell Physiol. 2005;204:113-23 pubmed
  34. Thomason R, Papiez J, Lee R, Szczarkowski W. Identification of unsuspected PNH-type cells in flow cytometric immunophenotypic analysis of peripheral blood and bone marrow. Am J Clin Pathol. 2004;122:128-34 pubmed
  35. Suskind D, Muench M. Searching for common stem cells of the hepatic and hematopoietic systems in the human fetal liver: CD34+ cytokeratin 7/8+ cells express markers for stellate cells. J Hepatol. 2004;40:261-8 pubmed
  36. Fierro M, Savoia P, Quaglino P, Novelli M, Barberis M, Bernengo M. Systemic therapy with cyclophosphamide and anti-CD20 antibody (rituximab) in relapsed primary cutaneous B-cell lymphoma: a report of 7 cases. J Am Acad Dermatol. 2003;49:281-7 pubmed
  37. Holada K, Mondoro T, Muller J, Vostal J. Increased expression of phosphatidylinositol-specific phospholipase C resistant prion proteins on the surface of activated platelets. Br J Haematol. 1998;103:276-82 pubmed
  38. Solomon K, Rudd C, Finberg R. The association between glycosylphosphatidylinositol-anchored proteins and heterotrimeric G protein alpha subunits in lymphocytes. Proc Natl Acad Sci U S A. 1996;93:6053-8 pubmed
  39. Koski C, Estep A, Sawant Mane S, Shin M, Highbarger L, Hansch G. Complement regulatory molecules on human myelin and glial cells: differential expression affects the deposition of activated complement proteins. J Neurochem. 1996;66:303-12 pubmed
  40. Tseng J, Hall S, Howard T, Ware R. Phenotypic and functional analysis of lymphocytes in paroxysmal nocturnal hemoglobinuria. Am J Hematol. 1995;50:244-53 pubmed
  41. Philbrick W, Palfree R, Maher S, Bridgett M, Sirlin S, Bothwell A. The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility. Eur J Immunol. 1990;20:87-92 pubmed