This is a Validated Antibody Database (VAD) review about human CD47, based on 26 published articles (read how Labome selects the articles), using CD47 antibody in all methods. It is aimed to help Labome visitors find the most suited CD47 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CD47 synonym: IAP; MER6; OA3

Knockout validation
Novus Biologicals
mouse monoclonal (B6H12.2)
  • western blot knockout validation; mouse; 1:1000; fig s2b
  • immunohistochemistry - frozen section; human; 1:50; fig 1b
  • immunocytochemistry; human; 1:50; loading ...; fig s1c
Novus Biologicals CD47 antibody (Novus Biologicals, NBP2-31106) was used in western blot knockout validation on mouse samples at 1:1000 (fig s2b), in immunohistochemistry - frozen section on human samples at 1:50 (fig 1b) and in immunocytochemistry on human samples at 1:50 (fig s1c). Nature (2016) ncbi
Invitrogen
mouse monoclonal (B6H12)
  • immunohistochemistry; human; 1:50; loading ...; fig 1a
Invitrogen CD47 antibody (Thermofisher, 14-0479-82) was used in immunohistochemistry on human samples at 1:50 (fig 1a). Nat Commun (2020) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human; loading ...; fig 1b
Invitrogen CD47 antibody (eBioscience, 17-00479-41) was used in flow cytometry on human samples (fig 1b). Nat Med (2019) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human; loading ...; fig 5b, 5c
Invitrogen CD47 antibody (eBiosciences, 17-0479-42) was used in flow cytometry on human samples (fig 5b, 5c). Cell Death Dis (2019) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human; fig 6a
In order to investigate the protective effect of single domain camelid antibodies against botulinum neurotoxin, Invitrogen CD47 antibody (Miltenyi Biotec, 46-0479-42) was used in flow cytometry on human samples (fig 6a). Nat Commun (2017) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; mouse; loading ...; fig e2a
In order to investigate the function of SLAMF in phagocytosis of haematopoietic tumour cells, Invitrogen CD47 antibody (eBioscience, B6H12) was used in flow cytometry on mouse samples (fig e2a). Nature (2017) ncbi
mouse monoclonal (2D3)
  • western blot; human
In order to discuss the role of CD47 modulation in treatment of chronic lymphocytic leukemia, Invitrogen CD47 antibody (eBioscience, 2D3) was used in western blot on human samples . PLoS Med (2015) ncbi
mouse monoclonal (B6H12)
  • blocking or activating experiments; mouse; 20 ug/ml
Invitrogen CD47 antibody (eBioscience, B6H12) was used in blocking or activating experiments on mouse samples at 20 ug/ml. J Neurosci (2009) ncbi
BioLegend
mouse monoclonal (CC2C6)
  • western blot; human; loading ...; fig 2b
BioLegend CD47 antibody (BioLegend, 323102) was used in western blot on human samples (fig 2b). Nat Med (2019) ncbi
mouse monoclonal (CC2C6)
  • flow cytometry; mouse; loading ...; fig e5d
In order to investigate the function of SLAMF in phagocytosis of haematopoietic tumour cells, BioLegend CD47 antibody (Biolegend, CC2C6) was used in flow cytometry on mouse samples (fig e5d). Nature (2017) ncbi
mouse monoclonal (CC2C6)
  • flow cytometry; human; fig 6b
In order to study the contribution of Wnt signaling to the development of functional proximal airway organoids, BioLegend CD47 antibody (BioLegend, 323110) was used in flow cytometry on human samples (fig 6b). Cell Stem Cell (2017) ncbi
Abcam
mouse monoclonal (B6H12)
  • flow cytometry; human; loading ...; fig s1
Abcam CD47 antibody (Abcam, ab134484) was used in flow cytometry on human samples (fig s1). Nat Commun (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:100; loading ...; fig 6k
  • western blot; human; 1:1000; loading ...; fig 6i
Abcam CD47 antibody (Abcam, ab175388) was used in immunohistochemistry on human samples at 1:100 (fig 6k) and in western blot on human samples at 1:1000 (fig 6i). Oncogene (2018) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human; 1:100; fig 3a
Abcam CD47 antibody (Abcam, ab134484) was used in flow cytometry on human samples at 1:100 (fig 3a). Sci Rep (2018) ncbi
Bio X Cell
mouse monoclonal (B6H12)
  • blocking or activating experiments; human; 1-25 ug/ml; loading ...; fig s2
Bio X Cell CD47 antibody (BioXcell, B6H12) was used in blocking or activating experiments on human samples at 1-25 ug/ml (fig s2). Nat Commun (2020) ncbi
mouse monoclonal (B6H12)
  • blocking or activating experiments; human; loading ...; fig st1
In order to investigate the effect of PD-1 expression on phagocytosis and tumour immunity, Bio X Cell CD47 antibody (BioXcell, B6.H12) was used in blocking or activating experiments on human samples (fig st1). Nature (2017) ncbi
Novus Biologicals
mouse monoclonal (B6H12.2)
  • western blot; human; loading ...; fig 2a
Novus Biologicals CD47 antibody (Novus Biologicals, NBP2-31106) was used in western blot on human samples (fig 2a). Nat Med (2019) ncbi
mouse monoclonal (B6H12.2)
  • western blot knockout validation; mouse; 1:1000; fig s2b
  • immunohistochemistry - frozen section; human; 1:50; fig 1b
  • immunocytochemistry; human; 1:50; loading ...; fig s1c
Novus Biologicals CD47 antibody (Novus Biologicals, NBP2-31106) was used in western blot knockout validation on mouse samples at 1:1000 (fig s2b), in immunohistochemistry - frozen section on human samples at 1:50 (fig 1b) and in immunocytochemistry on human samples at 1:50 (fig s1c). Nature (2016) ncbi
Bio-Rad
mouse monoclonal (1/1A4)
  • flow cytometry; mouse; 1:400; loading ...; fig s4b
Bio-Rad CD47 antibody (AbD Serotec, MCA2514GA) was used in flow cytometry on mouse samples at 1:400 (fig s4b). Nature (2016) ncbi
mouse monoclonal (BRIC126)
  • immunohistochemistry - paraffin section; pigs ; 1:100
  • western blot; pigs ; 1:1000
Bio-Rad CD47 antibody (serotec, MCA911) was used in immunohistochemistry - paraffin section on pigs samples at 1:100 and in western blot on pigs samples at 1:1000. Brain Res (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (BRIC 126)
  • western blot; human; fig s2
Santa Cruz Biotechnology CD47 antibody (Santa Cruz, sc-59079) was used in western blot on human samples (fig s2). Nature (2015) ncbi
mouse monoclonal (B6H12)
  • immunohistochemistry; human
  • western blot; human; fig 1
Santa Cruz Biotechnology CD47 antibody (Santa Cruz, sc-12730) was used in immunohistochemistry on human samples and in western blot on human samples (fig 1). Mol Oncol (2015) ncbi
BEI Resources
monoclonal (NA05-AG12)
  • immunocytochemistry; human; 1:100; loading ...; fig 5a
BEI Resources CD47 antibody (BEI Resources, NA05-AG12) was used in immunocytochemistry on human samples at 1:100 (fig 5a). J Virol (2017) ncbi
Vector Laboratories
  • immunocytochemistry; human; 1.4 ug/ml; fig 1
In order to investigate the role of Notch signaling in human basal cell differentiation, Vector Laboratories CD47 antibody (Vector Laboratories, VP-M657) was used in immunocytochemistry on human samples at 1.4 ug/ml (fig 1). PLoS ONE (2015) ncbi
BD Biosciences
mouse monoclonal (B6H12)
  • immunocytochemistry; human; loading ...; fig s1a
BD Biosciences CD47 antibody (BD Biosciences, 556046) was used in immunocytochemistry on human samples (fig s1a). Cell (2019) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human; loading ...; tbl 2
In order to investigate the role of cell surface 90 kDa heat shock protein in cytokine response, BD Biosciences CD47 antibody (BD Bioscience, B6412) was used in flow cytometry on human samples (tbl 2). J Leukoc Biol (2017) ncbi
mouse monoclonal (B6H12)
  • blocking or activating experiments; human; loading ...; fig 4g
BD Biosciences CD47 antibody (BD Biosciences, 556044) was used in blocking or activating experiments on human samples (fig 4g). PLoS ONE (2017) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human; tbl 3
In order to document and describe lymphocyte predominant cells from lymph nodes involved in nodular lymphocyte predominant Hodgkin lymphoma, BD Biosciences CD47 antibody (BD Biosciences, B6H12) was used in flow cytometry on human samples (tbl 3). Am J Pathol (2017) ncbi
mouse monoclonal (B6H12)
  • other; human; 500 ug/ml; fig 1
BD Biosciences CD47 antibody (Becton Dickinson, 556044) was used in other on human samples at 500 ug/ml (fig 1). J Extracell Vesicles (2016) ncbi
mouse monoclonal (B6H12)
  • immunohistochemistry - paraffin section; human; loading ...; fig s1l
  • flow cytometry; human; fig s4b
BD Biosciences CD47 antibody (BD Biosciences, B6H12) was used in immunohistochemistry - paraffin section on human samples (fig s1l) and in flow cytometry on human samples (fig s4b). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences CD47 antibody (BD, 556046) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human; fig s1
BD Biosciences CD47 antibody (BD Biosciences, 561261) was used in flow cytometry on human samples (fig s1). Nature (2015) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human
In order to discuss the role of CD47 modulation in treatment of chronic lymphocytic leukemia, BD Biosciences CD47 antibody (BD Biosciences, B6H12) was used in flow cytometry on human samples . PLoS Med (2015) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human
BD Biosciences CD47 antibody (BD Biosciences, B6H12) was used in flow cytometry on human samples . Oncotarget (2014) ncbi
mouse monoclonal (B6H12)
  • flow cytometry; human
BD Biosciences CD47 antibody (BD, 561594) was used in flow cytometry on human samples . Part Part Syst Charact (2013) ncbi
Articles Reviewed
  1. von Roemeling C, Wang Y, Qie Y, Yuan H, Zhao H, Liu X, et al. Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity. Nat Commun. 2020;11:1508 pubmed publisher
  2. Nakanishi M, Mitchell R, Benoit Y, Orlando L, Reid J, Shimada K, et al. Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells. Cell. 2019;177:910-924.e22 pubmed publisher
  3. Logtenberg M, Jansen J, Raaben M, Toebes M, Franke K, Brandsma A, et al. Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and a target for cancer immunotherapy. Nat Med. 2019;25:612-619 pubmed publisher
  4. Guillon J, Petit C, Moreau M, Toutain B, Henry C, Roche H, et al. Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment. Cell Death Dis. 2019;10:199 pubmed publisher
  5. Liu F, Dai M, Xu Q, Zhu X, Zhou Y, Jiang S, et al. SRSF10-mediated IL1RAP alternative splicing regulates cervical cancer oncogenesis via mIL1RAP-NF-κB-CD47 axis. Oncogene. 2018;37:2394-2409 pubmed publisher
  6. Paik E, O Neil A, Ng S, Sun C, Rubin L. Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture. Sci Rep. 2018;8:804 pubmed publisher
  7. Huang N, Pishesha N, Mukherjee J, Zhang S, Deshycka R, Sudaryo V, et al. Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin. Nat Commun. 2017;8:423 pubmed publisher
  8. Bzowska M, Nogieć A, Bania K, Zygmunt M, Zarebski M, Dobrucki J, et al. Involvement of cell surface 90 kDa heat shock protein (HSP90) in pattern recognition by human monocyte-derived macrophages. J Leukoc Biol. 2017;102:763-774 pubmed publisher
  9. King B, Hershkowitz D, Eisenhauer P, Weir M, Ziegler C, Russo J, et al. A Map of the Arenavirus Nucleoprotein-Host Protein Interactome Reveals that Junín Virus Selectively Impairs the Antiviral Activity of Double-Stranded RNA-Activated Protein Kinase (PKR). J Virol. 2017;91: pubmed publisher
  10. Gordon S, Maute R, Dulken B, Hutter G, George B, McCracken M, et al. PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity. Nature. 2017;545:495-499 pubmed publisher
  11. Chen J, Zhong M, Guo H, Davidson D, Mishel S, Lu Y, et al. SLAMF7 is critical for phagocytosis of haematopoietic tumour cells via Mac-1 integrin. Nature. 2017;544:493-497 pubmed publisher
  12. Kim S, Kim S, Bae D, Park S, Lee G, Park G, et al. Coordinated balance of Rac1 and RhoA plays key roles in determining phagocytic appetite. PLoS ONE. 2017;12:e0174603 pubmed publisher
  13. McCauley K, Hawkins F, Serra M, Thomas D, JACOB A, Kotton D. Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling. Cell Stem Cell. 2017;20:844-857.e6 pubmed publisher
  14. Fromm J, Thomas A, Wood B. Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting. Am J Pathol. 2017;187:304-317 pubmed publisher
  15. Kojima Y, Volkmer J, McKenna K, Civelek M, Lusis A, Miller C, et al. CD47-blocking antibodies restore phagocytosis and prevent atherosclerosis. Nature. 2016;536:86-90 pubmed
  16. Belov L, Matic K, Hallal S, Best O, Mulligan S, Christopherson R. Extensive surface protein profiles of extracellular vesicles from cancer cells may provide diagnostic signatures from blood samples. J Extracell Vesicles. 2016;5:25355 pubmed publisher
  17. Krampitz G, George B, Willingham S, Volkmer J, Weiskopf K, Jahchan N, et al. Identification of tumorigenic cells and therapeutic targets in pancreatic neuroendocrine tumors. Proc Natl Acad Sci U S A. 2016;113:4464-9 pubmed publisher
  18. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  19. Berkovits B, Mayr C. Alternative 3' UTRs act as scaffolds to regulate membrane protein localization. Nature. 2015;522:363-7 pubmed publisher
  20. Martínez Torres A, Quiney C, Attout T, Boullet H, Herbi L, Vela L, et al. CD47 agonist peptides induce programmed cell death in refractory chronic lymphocytic leukemia B cells via PLCγ1 activation: evidence from mice and humans. PLoS Med. 2015;12:e1001796 pubmed publisher
  21. Gomi K, Arbelaez V, Crystal R, Walters M. Activation of NOTCH1 or NOTCH3 signaling skews human airway basal cell differentiation toward a secretory pathway. PLoS ONE. 2015;10:e0116507 pubmed publisher
  22. Liu S, Lee W, Lai D, Wu S, Liu C, Tien H, et al. Honokiol confers immunogenicity by dictating calreticulin exposure, activating ER stress and inhibiting epithelial-to-mesenchymal transition. Mol Oncol. 2015;9:834-49 pubmed publisher
  23. Baccelli I, Stenzinger A, Vogel V, Pfitzner B, Klein C, Wallwiener M, et al. Co-expression of MET and CD47 is a novel prognosticator for survival of luminal breast cancer patients. Oncotarget. 2014;5:8147-60 pubmed
  24. Zhou X, Xie Q, Xi G, Keep R, Hua Y. Brain CD47 expression in a swine model of intracerebral hemorrhage. Brain Res. 2014;1574:70-6 pubmed publisher
  25. Raghunathan R, Mahesula S, Kancharla K, Janardhanan P, Jadhav Y, Nadeau R, et al. Anti-CRLF2 Antibody-Armored Biodegradable Nanoparticles for Childhood B-ALL. Part Part Syst Charact. 2013;30:355-364 pubmed
  26. Reed Geaghan E, Savage J, Hise A, Landreth G. CD14 and toll-like receptors 2 and 4 are required for fibrillar A{beta}-stimulated microglial activation. J Neurosci. 2009;29:11982-92 pubmed publisher