This is a Validated Antibody Database (VAD) review about human CD46, based on 17 published articles (read how Labome selects the articles), using CD46 antibody in all methods. It is aimed to help Labome visitors find the most suited CD46 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CD46 synonym: AHUS2; MCP; MIC10; TLX; TRA2.10

BioLegend
mouse monoclonal (MEM-258)
  • flow cytometry; pigs ; 1:5; loading ...; fig 4a
BioLegend CD46 antibody (BioLegend, 315304) was used in flow cytometry on pigs samples at 1:5 (fig 4a). Cells (2021) ncbi
rat monoclonal (TUG8)
  • immunohistochemistry; mouse; loading ...; fig 1e
BioLegend CD46 antibody (BioLegend, TUG8) was used in immunohistochemistry on mouse samples (fig 1e). Nat Commun (2021) ncbi
rat monoclonal (TUG8)
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 3e
In order to suggest that air-pollution-induced atopic dermatitis occurs through activation of AhR, BioLegend CD46 antibody (BioLegend, TUG8) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 3e). Nat Immunol (2017) ncbi
mouse monoclonal (TRA-2-10)
  • flow cytometry; human
BioLegend CD46 antibody (Biolegend, #352401) was used in flow cytometry on human samples . Am J Transplant (2015) ncbi
rat monoclonal (TUG8)
  • immunohistochemistry - frozen section; mouse
BioLegend CD46 antibody (BioLegend, TUG8) was used in immunohistochemistry - frozen section on mouse samples . Proc Natl Acad Sci U S A (2014) ncbi
rat monoclonal (TUG8)
In order to propose that basophils exert direct innate immune effector functions in the extracellular space, BioLegend CD46 antibody (BioLegend, TUG8) was used . J Immunol (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-10)
  • flow cytometry; human; loading ...; fig 5a
Santa Cruz Biotechnology CD46 antibody (SantaCruz, sc-166159) was used in flow cytometry on human samples (fig 5a). PLoS ONE (2014) ncbi
mouse monoclonal (C-10)
  • immunohistochemistry - paraffin section; human
Santa Cruz Biotechnology CD46 antibody (Santa Cruz Biotechnology, sc-166159) was used in immunohistochemistry - paraffin section on human samples . Arch Pathol Lab Med (2014) ncbi
mouse monoclonal (C-10)
  • immunohistochemistry - paraffin section; human; 1:150
  • western blot; human; 1:1000
Santa Cruz Biotechnology CD46 antibody (Santa Cruz Biotechnology, sc-166159) was used in immunohistochemistry - paraffin section on human samples at 1:150 and in western blot on human samples at 1:1000. FASEB J (2014) ncbi
mouse monoclonal (M177)
  • western blot; human; fig 7b
Santa Cruz Biotechnology CD46 antibody (Santa Cruz Biotechnology, M177) was used in western blot on human samples (fig 7b). Am J Physiol Gastrointest Liver Physiol (2014) ncbi
mouse monoclonal (M177)
  • western blot; human
Santa Cruz Biotechnology CD46 antibody (Santa Cruz Biotechnology, M177) was used in western blot on human samples . J Biol Chem (2014) ncbi
Invitrogen
mouse monoclonal (MEM-258)
  • flow cytometry; human; loading ...; fig 1
Invitrogen CD46 antibody (Thermo Fisher, MEM-258) was used in flow cytometry on human samples (fig 1). J Virol (2019) ncbi
mouse monoclonal (M177)
  • immunocytochemistry; human; fig 3
In order to study the essential alternative complement pathway components regulated by TNF and how they impair activation of protein c in human glomerular endothelial cells, Invitrogen CD46 antibody (Thermo Fisher, MA1-40183) was used in immunocytochemistry on human samples (fig 3). J Immunol (2016) ncbi
BD Biosciences
mouse monoclonal (E4.3)
  • immunohistochemistry; human; fig 2
BD Biosciences CD46 antibody (BD Biosciences, 555948) was used in immunohistochemistry on human samples (fig 2). Mol Vis (2016) ncbi
mouse monoclonal (E4.3)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences CD46 antibody (BD, 555949) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (E4.3)
  • flow cytometry; mouse
BD Biosciences CD46 antibody (BD Pharmingen, E4.3) was used in flow cytometry on mouse samples . Virus Res (2014) ncbi
mouse monoclonal (E4.3)
  • flow cytometry; human; fig S5
In order to use a new model which initiates alternate pathway activation on retinal pigment epithelial cells to investigate cell death, BD Biosciences CD46 antibody (BD Biosciences, clone E4.3) was used in flow cytometry on human samples (fig S5). Invest Ophthalmol Vis Sci (2014) ncbi
Articles Reviewed
  1. Tritschler H, Fischer K, Seissler J, Fiedler J, Halbgebauer R, Huber Lang M, et al. New Insights into Xenotransplantation for Cartilage Repair: Porcine Multi-Genetically Modified Chondrocytes as a Promising Cell Source. Cells. 2021;10: pubmed publisher
  2. Hiroyasu S, Zeglinski M, Zhao H, Pawluk M, Turner C, Kasprick A, et al. Granzyme B inhibition reduces disease severity in autoimmune blistering diseases. Nat Commun. 2021;12:302 pubmed publisher
  3. Nestić D, Uil T, Ma J, Roy S, Vellinga J, Baker A, et al. αvβ3 Integrin Is Required for Efficient Infection of Epithelial Cells with Human Adenovirus Type 26. J Virol. 2019;93: pubmed publisher
  4. Hidaka T, Ogawa E, Kobayashi E, Suzuki T, Funayama R, Nagashima T, et al. The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin. Nat Immunol. 2017;18:64-73 pubmed publisher
  5. Kochounian H, Zhang Z, Spee C, Hinton D, Fong H. Targeting of exon VI-skipping human RGR-opsin to the plasma membrane of pigment epithelium and co-localization with terminal complement complex C5b-9. Mol Vis. 2016;22:213-23 pubmed
  6. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  7. Sartain S, Turner N, Moake J. TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human Glomerular Endothelial Cells. J Immunol. 2016;196:832-45 pubmed publisher
  8. Thomas K, Valenzuela N, Gjertson D, Mulder A, Fishbein M, Parry G, et al. An Anti-C1s Monoclonal, TNT003, Inhibits Complement Activation Induced by Antibodies Against HLA. Am J Transplant. 2015;15:2037-49 pubmed publisher
  9. Prusty B, Siegl C, Gulve N, Mori Y, Rudel T. GP96 interacts with HHV-6 during viral entry and directs it for cellular degradation. PLoS ONE. 2014;9:e113962 pubmed publisher
  10. Schwartz C, Turqueti Neves A, Hartmann S, Yu P, Nimmerjahn F, Voehringer D. Basophil-mediated protection against gastrointestinal helminths requires IgE-induced cytokine secretion. Proc Natl Acad Sci U S A. 2014;111:E5169-77 pubmed publisher
  11. Shang Y, Chai N, Gu Y, Ding L, Yang Y, Zhou J, et al. Systematic immunohistochemical analysis of the expression of CD46, CD55, and CD59 in colon cancer. Arch Pathol Lab Med. 2014;138:910-9 pubmed publisher
  12. Gu H, Mickler E, Cummings O, Sandusky G, Weber D, Gracon A, et al. Crosstalk between TGF-?1 and complement activation augments epithelial injury in pulmonary fibrosis. FASEB J. 2014;28:4223-34 pubmed publisher
  13. M Parrula M, Fernandez S, Landes K, Huey D, Lairmore M, Niewiesk S. Success of measles virotherapy in ATL depends on type I interferon secretion and responsiveness. Virus Res. 2014;189:206-13 pubmed publisher
  14. Morshed M, Hlushchuk R, Simon D, Walls A, Obata Ninomiya K, Karasuyama H, et al. NADPH oxidase-independent formation of extracellular DNA traps by basophils. J Immunol. 2014;192:5314-23 pubmed publisher
  15. Wu Y, Wang Y, Qin F, Wang Z, Wang Y, Yang Y, et al. CD55 limits sensitivity to complement-dependent cytolysis triggered by heterologous expression of ?-gal xenoantigen in colon tumor cells. Am J Physiol Gastrointest Liver Physiol. 2014;306:G1056-64 pubmed publisher
  16. Yang P, Baciu P, Kerrigan B, Etheridge M, Sung E, Toimil B, et al. Retinal pigment epithelial cell death by the alternative complement cascade: role of membrane regulatory proteins, calcium, PKC, and oxidative stress. Invest Ophthalmol Vis Sci. 2014;55:3012-21 pubmed publisher
  17. Du Y, Teng X, Wang N, Zhang X, Chen J, Ding P, et al. NF-?B and enhancer-binding CREB protein scaffolded by CREB-binding protein (CBP)/p300 proteins regulate CD59 protein expression to protect cells from complement attack. J Biol Chem. 2014;289:2711-24 pubmed publisher