This is a Validated Antibody Database (VAD) review about human CD43, based on 22 published articles (read how Labome selects the articles), using CD43 antibody in all methods. It is aimed to help Labome visitors find the most suited CD43 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CD43 synonym: CD43; GALGP; GPL115; LSN; leukosialin; galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (gpL115, leukosialin, CD43)

Invitrogen
mouse monoclonal (L10)
  • flow cytometry; human; tbl 3
In order to document and describe lymphocyte predominant cells from lymph nodes involved in nodular lymphocyte predominant Hodgkin lymphoma, Invitrogen CD43 antibody (Caltag, L10) was used in flow cytometry on human samples (tbl 3). Am J Pathol (2017) ncbi
mouse monoclonal (L10)
  • flow cytometry; human; loading ...; fig s5b
In order to investigate the role of CHCHD2 in the differentiation of human induced pluripotent stem cell, Invitrogen CD43 antibody (Thermo Fisher, MHCD4301) was used in flow cytometry on human samples (fig s5b). J Cell Biol (2016) ncbi
mouse monoclonal (L10)
  • flow cytometry; human; fig 1
In order to find cell surface markers that discriminate CLL-type MBL from CLL, Invitrogen CD43 antibody (Caltag, MHCD4305) was used in flow cytometry on human samples (fig 1). Cytometry B Clin Cytom (2010) ncbi
Miltenyi Biotec
mouse monoclonal (DF-T1)
  • flow cytometry; human; loading ...; fig 2c
In order to use CRISPR/Cas9 editing to generate reagents to study the role of IRF8 in human hematopoiesis, Miltenyi Biotec CD43 antibody (Miltenyi Biotech, DF-T1) was used in flow cytometry on human samples (fig 2c). Stem Cells (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (MT1)
  • flow cytometry; human; 1:25; loading ...; fig 1b
In order to study how retinoic acid-induced HOXA gene expression controls hematopoietic stem/progenitor cells fate, identify, and function, Santa Cruz Biotechnology CD43 antibody (SCB, 51727) was used in flow cytometry on human samples at 1:25 (fig 1b). Nat Cell Biol (2016) ncbi
BioLegend
mouse monoclonal (CD43-10G7)
  • flow cytometry; human; tbl 2
BioLegend CD43 antibody (Biolegend, 343206) was used in flow cytometry on human samples (tbl 2). Exp Cell Res (2015) ncbi
Dako
mouse monoclonal (DF-T1)
  • immunohistochemistry - paraffin section; human; 1:2500; loading ...; fig 3c
Dako CD43 antibody (Dako, M0786) was used in immunohistochemistry - paraffin section on human samples at 1:2500 (fig 3c). Oncol Lett (2016) ncbi
mouse monoclonal (DF-T1)
  • immunohistochemistry - paraffin section; human; 1:150
In order to describe a case of marginal zone lymphoma with extensive emperipolesis, Dako CD43 antibody (Dako, DF-T1) was used in immunohistochemistry - paraffin section on human samples at 1:150. Cytopathology (2016) ncbi
Beckman Coulter
mouse monoclonal (DFT1)
  • flow cytometry; human
In order to assess the efficacy of using flow cytometry immunophenotyping with fine-needle aspiration cytology for the diagnosis of thyroid lymphoma, Beckman Coulter CD43 antibody (Coulter, DFT1) was used in flow cytometry on human samples . Cytometry B Clin Cytom (2015) ncbi
mouse monoclonal (DFT1)
  • flow cytometry; human; tbl 1
In order to compare fine needle aspiration cytology with flow cytometry immunophenotyping for the diagnosis of lymphoproliferative processes in the salivary glands, Beckman Coulter CD43 antibody (Coulter, clone DFT1) was used in flow cytometry on human samples (tbl 1). Cytopathology (2014) ncbi
BD Biosciences
mouse monoclonal (1G10)
  • flow cytometry; human; 1:100; loading ...; fig 6a
BD Biosciences CD43 antibody (BD Bioscience, 555474) was used in flow cytometry on human samples at 1:100 (fig 6a). Nat Commun (2018) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; fig s1b
BD Biosciences CD43 antibody (BD, 1G10) was used in flow cytometry on human samples (fig s1b). Nature (2017) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; loading ...; fig 2c
In order to use CRISPR/Cas9 editing to generate reagents to study the role of IRF8 in human hematopoiesis, BD Biosciences CD43 antibody (BD Bioscience, 1G10) was used in flow cytometry on human samples (fig 2c). Stem Cells (2017) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; loading ...; fig st1
BD Biosciences CD43 antibody (BD Biosciences, 555475) was used in flow cytometry on human samples (fig st1). PLoS ONE (2016) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; 1:20; loading ...; fig 1b
In order to study how retinoic acid-induced HOXA gene expression controls hematopoietic stem/progenitor cells fate, identify, and function, BD Biosciences CD43 antibody (BD, 1G10) was used in flow cytometry on human samples at 1:20 (fig 1b). Nat Cell Biol (2016) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; fig 1
BD Biosciences CD43 antibody (BD Biosciences, 555475) was used in flow cytometry on human samples (fig 1). Stem Cell Reports (2016) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences CD43 antibody (BD, 560199) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (1G10)
  • other; human; loading ...; fig st1
  • flow cytometry; human; loading ...; fig st3
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences CD43 antibody (BD Biosciences, IG10) was used in other on human samples (fig st1) and in flow cytometry on human samples (fig st3). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (L60)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences CD43 antibody (BD, L60) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; fig 4
BD Biosciences CD43 antibody (BD Pharmingen, 555475) was used in flow cytometry on human samples (fig 4). Stem Cell Reports (2016) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; loading ...; fig s3
BD Biosciences CD43 antibody (BD Biosciences, 1G10) was used in flow cytometry on human samples (fig s3). Sci Transl Med (2015) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; loading ...; fig 1c
In order to define a differentiation system to generate definitive hematopoietic multipotent progenitor cells from hemato-endothelial progenitors via the endothelial monolayer, BD Biosciences CD43 antibody (BD Biosciences, 1G10) was used in flow cytometry on human samples (fig 1c). J Cell Physiol (2016) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; fig 1
BD Biosciences CD43 antibody (BD Biosciences, 1G10) was used in flow cytometry on human samples (fig 1). Int Immunol (2015) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; fig 1
BD Biosciences CD43 antibody (BD Biosciences, 555475) was used in flow cytometry on human samples (fig 1). Stem Cell Reports (2015) ncbi
mouse monoclonal (1G10)
  • flow cytometry; human; fig 3
BD Biosciences CD43 antibody (BD Pharmingen, 1G10) was used in flow cytometry on human samples (fig 3). Blood (2015) ncbi
Articles Reviewed
  1. Murakami T, Kim J, Li Y, Green G, Shikanov A, Ono A. Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions. Nat Commun. 2018;9:2436 pubmed publisher
  2. Sugimura R, Jha D, Han A, Soria Valles C, da Rocha E, Lu Y, et al. Haematopoietic stem and progenitor cells from human pluripotent stem cells. Nature. 2017;545:432-438 pubmed publisher
  3. Sontag S, Förster M, Qin J, Wanek P, Mitzka S, Schüler H, et al. Modelling IRF8 Deficient Human Hematopoiesis and Dendritic Cell Development with Engineered iPS Cells. Stem Cells. 2017;35:898-908 pubmed publisher
  4. Fromm J, Thomas A, Wood B. Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting. Am J Pathol. 2017;187:304-317 pubmed publisher
  5. Zhu L, Gómez Durán A, Saretzki G, Jin S, Tilgner K, Melguizo Sanchís D, et al. The mitochondrial protein CHCHD2 primes the differentiation potential of human induced pluripotent stem cells to neuroectodermal lineages. J Cell Biol. 2016;215:187-202 pubmed
  6. Clavarino G, Delouche N, Vettier C, Laurin D, Pernollet M, Raskovalova T, et al. Novel Strategy for Phenotypic Characterization of Human B Lymphocytes from Precursors to Effector Cells by Flow Cytometry. PLoS ONE. 2016;11:e0162209 pubmed publisher
  7. Fu T, Yang W, Zhang X, Xu X. Peripheral T-cell lymphoma unspecified type presenting with a pneumothorax as the initial manifestation: A case report and literature review. Oncol Lett. 2016;11:4069-4076 pubmed
  8. Dou D, Calvanese V, Sierra M, Nguyen A, Minasian A, Saarikoski P, et al. Medial HOXA genes demarcate haematopoietic stem cell fate during human development. Nat Cell Biol. 2016;18:595-606 pubmed publisher
  9. Saxena S, Ronn R, Guibentif C, Moraghebi R, Woods N. Cyclic AMP Signaling through Epac Axis Modulates Human Hemogenic Endothelium and Enhances Hematopoietic Cell Generation. Stem Cell Reports. 2016;6:692-703 pubmed publisher
  10. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  11. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  12. Kyttälä A, Moraghebi R, Valensisi C, Kettunen J, Andrus C, Pasumarthy K, et al. Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential. Stem Cell Reports. 2016;6:200-12 pubmed publisher
  13. Li R, Rezk A, Miyazaki Y, Hilgenberg E, Touil H, Shen P, et al. Proinflammatory GM-CSF-producing B cells in multiple sclerosis and B cell depletion therapy. Sci Transl Med. 2015;7:310ra166 pubmed publisher
  14. Bai H, Liu Y, Xie Y, Hoyle D, Brodsky R, Cheng L, et al. Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells. J Cell Physiol. 2016;231:1065-76 pubmed publisher
  15. Liu J, Brzeszczynska J, Samuel K, Black J, Palakkan A, Anderson R, et al. Efficient episomal reprogramming of blood mononuclear cells and differentiation to hepatocytes with functional drug metabolism. Exp Cell Res. 2015;338:203-13 pubmed publisher
  16. Inui M, Hirota S, Hirano K, Fujii H, Sugahara Tobinai A, Ishii T, et al. Human CD43+ B cells are closely related not only to memory B cells phenotypically but also to plasmablasts developmentally in healthy individuals. Int Immunol. 2015;27:345-55 pubmed publisher
  17. Rönn R, Guibentif C, Moraghebi R, Chaves P, Saxena S, Garcia B, et al. Retinoic acid regulates hematopoietic development from human pluripotent stem cells. Stem Cell Reports. 2015;4:269-81 pubmed publisher
  18. Meykler S, Baloch Z, Barroeta J. A case of marginal zone lymphoma with extensive emperipolesis diagnosed on pleural effusion cytology with immunocytochemistry and flow cytometry. Cytopathology. 2016;27:70-2 pubmed publisher
  19. de Masson A, Bouaziz J, Le Buanec H, Robin M, O Meara A, Parquet N, et al. CD24(hi)CD27⁺ and plasmablast-like regulatory B cells in human chronic graft-versus-host disease. Blood. 2015;125:1830-9 pubmed publisher
  20. Stacchini A, Pacchioni D, Demurtas A, Aliberti S, Cassenti A, Isolato G, et al. Utilility of flow cytometry as ancillary study to improve the cytologic diagnosis of thyroid lymphomas. Cytometry B Clin Cytom. 2015;88:320-9 pubmed publisher
  21. Stacchini A, Aliberti S, Pacchioni D, Demurtas A, Isolato G, Gazzera C, et al. Flow cytometry significantly improves the diagnostic value of fine needle aspiration cytology of lymphoproliferative lesions of salivary glands. Cytopathology. 2014;25:231-40 pubmed publisher
  22. Rawstron A, Shingles J, de Tute R, Bennett F, Jack A, Hillmen P. Chronic lymphocytic leukaemia (CLL) and CLL-type monoclonal B-cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing. Cytometry B Clin Cytom. 2010;78 Suppl 1:S42-6 pubmed publisher