This is a Validated Antibody Database (VAD) review about human CD22, based on 22 published articles (read how Labome selects the articles), using CD22 antibody in all methods. It is aimed to help Labome visitors find the most suited CD22 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CD22 synonym: SIGLEC-2; SIGLEC2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; sialic acid-binding Ig-like lectin 2

BioLegend
mouse monoclonal (HIB22)
  • flow cytometry; human; loading ...; fig 1a
BioLegend CD22 antibody (Biolegend, 302502) was used in flow cytometry on human samples (fig 1a). Cell (2018) ncbi
mouse monoclonal (HIB22)
  • flow cytometry; human; loading ...; fig s4b
BioLegend CD22 antibody (Biolegend, 302514) was used in flow cytometry on human samples (fig s4b). J Clin Invest (2018) ncbi
mouse monoclonal (HIB22)
  • flow cytometry; human
BioLegend CD22 antibody (Biolegend, HIB22) was used in flow cytometry on human samples . PLoS ONE (2014) ncbi
Invitrogen
mouse monoclonal (RFB-4)
  • flow cytometry; human; tbl 2
In order to test if flow cytometry can be used to find mantle cell lymphoma, Invitrogen CD22 antibody (Invitrogen, RFB4) was used in flow cytometry on human samples (tbl 2). Cytometry B Clin Cytom (2014) ncbi
mouse monoclonal (RFB-4)
  • flow cytometry; human; tbl 1
In order to evaluate the diagnostic value of CD123 expression in B-cell disorders with circulating hairy and villous lymphocytes, Invitrogen CD22 antibody (Caltag, RFB4) was used in flow cytometry on human samples (tbl 1). Haematologica (2004) ncbi
Abcam
mouse monoclonal (RFB4)
  • flow cytometry; dog; loading ...
In order to evaluate the use of valspodar to treat canine B-cell lymphoma, Abcam CD22 antibody (Abcam, Ab23620) was used in flow cytometry on dog samples . F1000Res (2015) ncbi
Beckman Coulter
mouse monoclonal (SJ10.1H11)
  • flow cytometry; human; tbl 3
In order to document and describe lymphocyte predominant cells from lymph nodes involved in nodular lymphocyte predominant Hodgkin lymphoma, Beckman Coulter CD22 antibody (Beckman Coulter, SJ10.1H11) was used in flow cytometry on human samples (tbl 3). Am J Pathol (2017) ncbi
mouse monoclonal (SJ10.1H11)
  • flow cytometry; human; fig 1e
In order to measure the expression of antigens on malignant human plasma cells that have exhibited promise in targeted cancer therapy, Beckman Coulter CD22 antibody (Beckman Coulter, SJ10) was used in flow cytometry on human samples (fig 1e). Cytometry B Clin Cytom (2017) ncbi
mouse monoclonal (SJ10.1H11)
  • flow cytometry; human; tbl 4
In order to discuss how to diagnosis hematolymphoid neoplasms using flow cytometry, Beckman Coulter CD22 antibody (Beckman Coulter, SJ10.1H11) was used in flow cytometry on human samples (tbl 4). Cytometry B Clin Cytom (2015) ncbi
mouse monoclonal (SJ10.1H11)
  • flow cytometry; human
Beckman Coulter CD22 antibody (Beckman Coulter, IM3704) was used in flow cytometry on human samples . Contemp Oncol (Pozn) (2014) ncbi
BD Biosciences
mouse monoclonal (S-HCL-1)
  • flow cytometry; human; loading ...; fig st12
In order to identify new types of human blood dendritic cells, monocytes, and progenitors through single-cell RNA-seq, BD Biosciences CD22 antibody (BD, S-HCL-1) was used in flow cytometry on human samples (fig st12). Science (2017) ncbi
mouse monoclonal (HIB22)
  • flow cytometry; human; loading ...; fig 4b
BD Biosciences CD22 antibody (BD, HIB22) was used in flow cytometry on human samples (fig 4b). Front Immunol (2017) ncbi
mouse monoclonal (S-HCL-1)
  • flow cytometry; human; loading ...; fig st1
BD Biosciences CD22 antibody (BD Biosciences, 347577) was used in flow cytometry on human samples (fig st1). PLoS ONE (2016) ncbi
mouse monoclonal (S-HCL-1)
  • flow cytometry; human; fig 1
BD Biosciences CD22 antibody (BD Biosciences, 347577) was used in flow cytometry on human samples (fig 1). Nat Commun (2016) ncbi
mouse monoclonal (HIB22)
  • other; human; 500 ug/ml; fig 2
BD Biosciences CD22 antibody (Becton Dickinson, 555423) was used in other on human samples at 500 ug/ml (fig 2). J Extracell Vesicles (2016) ncbi
mouse monoclonal (HIB22)
  • flow cytometry; human; loading ...; fig 2a
BD Biosciences CD22 antibody (BD Biosciences, HIB22) was used in flow cytometry on human samples (fig 2a). J Immunol (2016) ncbi
mouse monoclonal (S-HCL-1)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences CD22 antibody (BD, 347577) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (S-HCL-1)
  • other; human; loading ...; fig st1
  • flow cytometry; human; loading ...; fig st3
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences CD22 antibody (BD Biosciences, S-HCL-1) was used in other on human samples (fig st1) and in flow cytometry on human samples (fig st3). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (HIB22)
  • flow cytometry; human
BD Biosciences CD22 antibody (BD, HIB22) was used in flow cytometry on human samples . Nature (2015) ncbi
mouse monoclonal (S-HCL-1)
  • flow cytometry; human; loading ...; fig 2c
BD Biosciences CD22 antibody (Becton Dickinson, S-HCL-1) was used in flow cytometry on human samples (fig 2c). Clin Immunol (2014) ncbi
Sigma-Aldrich
mouse monoclonal (HIB22)
  • immunocytochemistry; human; 1 ug/ml
In order to investigate the subcellular trafficking and recycle of CD22, Sigma-Aldrich CD22 antibody (Sigma, HIB22) was used in immunocytochemistry on human samples at 1 ug/ml. Traffic (2014) ncbi
EMD Millipore
mouse monoclonal (RFB4)
  • immunohistochemistry; human
EMD Millipore CD22 antibody (Millipore, RFB4) was used in immunohistochemistry on human samples . Arthritis Rheumatol (2014) ncbi
Articles Reviewed
  1. Olin A, Henckel E, Chen Y, Lakshmikanth T, Pou C, Mikes J, et al. Stereotypic Immune System Development in Newborn Children. Cell. 2018;174:1277-1292.e14 pubmed publisher
  2. Burton A, Pallett L, McCoy L, Suveizdyte K, Amin O, Swadling L, et al. Circulating and intrahepatic antiviral B cells are defective in hepatitis B. J Clin Invest. 2018;128:4588-4603 pubmed publisher
  3. Villani A, Satija R, Reynolds G, Sarkizova S, Shekhar K, Fletcher J, et al. Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. Science. 2017;356: pubmed publisher
  4. Ito D, Childress M, Mason N, Winter A, O BRIEN T, Henson M, et al. A double blinded, placebo-controlled pilot study to examine reduction of CD34 +/CD117 +/CD133 + lymphoma progenitor cells and duration of remission induced by neoadjuvant valspodar in dogs with large B-cell lymphoma. F1000Res. 2015;4:42 pubmed publisher
  5. Canté Barrett K, Mendes R, Li Y, Vroegindeweij E, Pike Overzet K, Wabeke T, et al. Loss of CD44dim Expression from Early Progenitor Cells Marks T-Cell Lineage Commitment in the Human Thymus. Front Immunol. 2017;8:32 pubmed publisher
  6. Fromm J, Thomas A, Wood B. Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting. Am J Pathol. 2017;187:304-317 pubmed publisher
  7. Clavarino G, Delouche N, Vettier C, Laurin D, Pernollet M, Raskovalova T, et al. Novel Strategy for Phenotypic Characterization of Human B Lymphocytes from Precursors to Effector Cells by Flow Cytometry. PLoS ONE. 2016;11:e0162209 pubmed publisher
  8. Jacoby E, Nguyen S, Fountaine T, Welp K, Gryder B, Qin H, et al. CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity. Nat Commun. 2016;7:12320 pubmed publisher
  9. Belov L, Matic K, Hallal S, Best O, Mulligan S, Christopherson R. Extensive surface protein profiles of extracellular vesicles from cancer cells may provide diagnostic signatures from blood samples. J Extracell Vesicles. 2016;5:25355 pubmed publisher
  10. Li H, Borrego F, Nagata S, Tolnay M. Fc Receptor-like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue-like Memory B Cells. J Immunol. 2016;196:4064-74 pubmed publisher
  11. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  12. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  13. Lisenko K, Schönland S, Hegenbart U, Wallenwein K, Braun U, Mai E, et al. Potential therapeutic targets in plasma cell disorders: A flow cytometry study. Cytometry B Clin Cytom. 2017;92:145-152 pubmed publisher
  14. Fromm J, Tagliente D, Shaver A, Neppalli V, Craig F. Case study interpretation: Report from the ICCS Annual Meeting, Seattle, 2014. Cytometry B Clin Cytom. 2015;88:413-24 pubmed publisher
  15. Chen Z, Shojaee S, Buchner M, Geng H, Lee J, Klemm L, et al. Signalling thresholds and negative B-cell selection in acute lymphoblastic leukaemia. Nature. 2015;521:357-61 pubmed publisher
  16. Chovancová J, Bernard T, Stehlíková O, Sálek D, Janíková A, Mayer J, et al. Detection of Minimal Residual Disease in Mantle Cell Lymphoma. Establishment of Novel 8-Color Flow Cytometry Approach. Cytometry B Clin Cytom. 2014;: pubmed publisher
  17. Jovanovic D, Djurdjevic P, Andjelkovic N, Zivić L. Possible role of CD22, CD79b and CD20 expression in distinguishing small lymphocytic lymphoma from chronic lymphocytic leukemia. Contemp Oncol (Pozn). 2014;18:29-33 pubmed publisher
  18. Rossi E, Chang C, Goldenberg D. Anti-CD22/CD20 Bispecific antibody with enhanced trogocytosis for treatment of Lupus. PLoS ONE. 2014;9:e98315 pubmed publisher
  19. de Hair M, van de Sande M, Ramwadhdoebe T, Hansson M, Landewe R, van der Leij C, et al. Features of the synovium of individuals at risk of developing rheumatoid arthritis: implications for understanding preclinical rheumatoid arthritis. Arthritis Rheumatol. 2014;66:513-22 pubmed publisher
  20. Doi H, Tanoue S, Kaplan D. Peripheral CD27-CD21- B-cells represent an exhausted lymphocyte population in hepatitis C cirrhosis. Clin Immunol. 2014;150:184-91 pubmed publisher
  21. Ingle G, Scales S. DropArray™, a wall-less 96-well plate for uptake and immunofluorescence microscopy, confirms CD22 recycles. Traffic. 2014;15:255-72 pubmed publisher
  22. del Giudice I, Matutes E, Morilla R, Morilla A, Owusu Ankomah K, Rafiq F, et al. The diagnostic value of CD123 in B-cell disorders with hairy or villous lymphocytes. Haematologica. 2004;89:303-8 pubmed