This is a Validated Antibody Database (VAD) review about human CD21, based on 40 published articles (read how Labome selects the articles), using CD21 antibody in all methods. It is aimed to help Labome visitors find the most suited CD21 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CD21 synonym: C3DR; CD21; CVID7; SLEB9; complement receptor type 2; EBV receptor; complement component (3d/Epstein Barr virus) receptor 2; complement component 3d receptor 2

BioLegend
mouse monoclonal (Bu32)
  • flow cytometry; human; 1:100; loading ...; fig 4d
BioLegend CD21 antibody (Biolegend, 354908) was used in flow cytometry on human samples at 1:100 (fig 4d). elife (2019) ncbi
mouse monoclonal (Bu32)
  • flow cytometry; human; loading ...; fig s1d
In order to study inflammasome activation during primary Epstein-Barr virus infection, BioLegend CD21 antibody (BioLegend, 354911) was used in flow cytometry on human samples (fig s1d). PLoS ONE (2017) ncbi
mouse monoclonal (Bu32)
  • flow cytometry; human; loading ...; fig st9
In order to characterize American patients with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy, BioLegend CD21 antibody (BioLegend, Bu32) was used in flow cytometry on human samples (fig st9). JCI Insight (2016) ncbi
Invitrogen
mouse monoclonal (2G9)
  • immunohistochemistry - paraffin section; human; loading ...; fig 3e
In order to investigate the tumor microenvironment in tertiary lymphoid organs of prostate cancer patients, Invitrogen CD21 antibody (Thermo Fisher Scientific, 2G9) was used in immunohistochemistry - paraffin section on human samples (fig 3e). Front Immunol (2017) ncbi
mouse monoclonal (HB5)
  • flow cytometry; human; tbl 2
In order to test if flow cytometry can be used to find mantle cell lymphoma, Invitrogen CD21 antibody (eBioscience, HB5) was used in flow cytometry on human samples (tbl 2). Cytometry B Clin Cytom (2014) ncbi
mouse monoclonal (2G9)
  • immunohistochemistry - paraffin section; human; loading ...; fig 2c
  • immunohistochemistry; human
In order to study three clinical cases of follicular dendritic cell sarcoma, Invitrogen CD21 antibody (Thermo Scientific, 2G9) was used in immunohistochemistry - paraffin section on human samples (fig 2c) and in immunohistochemistry on human samples . Hum Pathol (2012) ncbi
mouse monoclonal (2G9)
  • immunohistochemistry - paraffin section; human
  • immunohistochemistry; human
In order to investigate the clinical and pathological features in a girl with common variable immunodeficiency, Invitrogen CD21 antibody (Thermo, 2G9) was used in immunohistochemistry - paraffin section on human samples and in immunohistochemistry on human samples . Int J Surg Pathol (2014) ncbi
Abcam
rabbit monoclonal (EP3093)
  • immunohistochemistry - paraffin section; African green monkey; 1:200
  • immunohistochemistry; African green monkey; loading ...; fig 53
In order to outline the protocols for antibodies used for immunohistochemical studies, Abcam CD21 antibody (Abcam, ab75985) was used in immunohistochemistry - paraffin section on African green monkey samples at 1:200 and in immunohistochemistry on African green monkey samples (fig 53). J Toxicol Pathol (2017) ncbi
rabbit monoclonal (EP3093)
  • immunohistochemistry - frozen section; human; fig s4c
Abcam CD21 antibody (Abcam, ab75985) was used in immunohistochemistry - frozen section on human samples (fig s4c). Arthritis Res Ther (2016) ncbi
rabbit monoclonal (EP3093)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 3a
Abcam CD21 antibody (Abcam, ab75985) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 3a). BMC Cancer (2015) ncbi
Bio-Rad
mouse monoclonal (LB21)
  • immunohistochemistry - frozen section; human; 1:100; loading ...; fig 3b
In order to study Hsp-70 in lymphoid tissue and plasma of acute stroke patients, Bio-Rad CD21 antibody (Serotec, MCA1195) was used in immunohistochemistry - frozen section on human samples at 1:100 (fig 3b). J Neuroimmunol (2014) ncbi
Beckman Coulter
mouse monoclonal (BL13)
  • flow cytometry; human; loading ...; tbl 1
In order to report the function of CD70-CD27 signaling in patients infected with Epstein-Barr virus, Beckman Coulter CD21 antibody (Beckman Coulter, BL13) was used in flow cytometry on human samples (tbl 1). J Exp Med (2017) ncbi
mouse monoclonal (BL13)
  • flow cytometry; human; tbl 3
In order to document and describe lymphocyte predominant cells from lymph nodes involved in nodular lymphocyte predominant Hodgkin lymphoma, Beckman Coulter CD21 antibody (Beckman Coulter, BL13) was used in flow cytometry on human samples (tbl 3). Am J Pathol (2017) ncbi
mouse monoclonal (BL13)
  • flow cytometry; human; loading ...; fig s1b
In order to compare expression and function of the CD300 family of receptors between neonatal and adult immune cells, Beckman Coulter CD21 antibody (Beckman-Coulter, BL13) was used in flow cytometry on human samples (fig s1b). Sci Rep (2016) ncbi
mouse monoclonal (BL13)
  • flow cytometry; human
Beckman Coulter CD21 antibody (Beckman Coulter, IM0473U)) was used in flow cytometry on human samples . Contemp Oncol (Pozn) (2014) ncbi
mouse monoclonal (BL13)
  • flow cytometry; common squirrel monkey
In order to discuss available reagents for studying the neotropical primate squirrel monkey, Beckman Coulter CD21 antibody (Beckman, BL13) was used in flow cytometry on common squirrel monkey samples . J Immunol Methods (2005) ncbi
Dako
mouse monoclonal (1F8)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 2a
In order to characterize cases of follicular dendritic cell sarcoma, Dako CD21 antibody (Dako, 1F8) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 2a). Ann Diagn Pathol (2016) ncbi
mouse monoclonal (1F8)
  • immunohistochemistry; human; 1:25
Dako CD21 antibody (Dako, 1F8) was used in immunohistochemistry on human samples at 1:25. Hum Pathol (2015) ncbi
mouse monoclonal (1F8)
  • immunohistochemistry; human; 1:25; fig 1
In order to investigate the B cell compartment of patients with chronic lymphocytic leukemia/small lymphocytic lymphoma, Dako CD21 antibody (Dako, 1F8) was used in immunohistochemistry on human samples at 1:25 (fig 1). Leuk Lymphoma (2015) ncbi
mouse monoclonal (1F8)
  • immunohistochemistry - frozen section; human; 1:20; fig 1
In order to examine Cx43 channels in reactive follicular dendritic cell development and follicular lymphomas, Dako CD21 antibody (Dako, clone: 1F8) was used in immunohistochemistry - frozen section on human samples at 1:20 (fig 1). J Immunol Res (2015) ncbi
mouse monoclonal (1F8)
  • immunohistochemistry - paraffin section; human
In order to describe the features of plasma cells in patients with granulomatosis with polyangiitis, Dako CD21 antibody (Dako, 1 F8) was used in immunohistochemistry - paraffin section on human samples . Arthritis Res Ther (2014) ncbi
BD Biosciences
mouse monoclonal (B-ly4)
  • flow cytometry; human; loading ...; fig 1c
BD Biosciences CD21 antibody (BD Biosciences, 561374) was used in flow cytometry on human samples (fig 1c). J Clin Invest (2019) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human; loading ...; fig 3b
BD Biosciences CD21 antibody (BD, 561767) was used in flow cytometry on human samples (fig 3b). J Clin Invest (2018) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human; loading ...; fig 9a
BD Biosciences CD21 antibody (BD, 551064) was used in flow cytometry on human samples (fig 9a). J Virol (2018) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human; loading ...
In order to discuss how mutations in PIK3CD and PIK3R1 cause activated PI3K-delta syndrome, BD Biosciences CD21 antibody (BD Biosciences, B-ly4) was used in flow cytometry on human samples . Clin Immunol (2017) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human; loading ...
In order to show T cell immunoglobulin and ITIM domain expression increases over time despite early initiation of antiretroviral treatment, BD Biosciences CD21 antibody (BD Bioscience, B-ly4) was used in flow cytometry on human samples . Sci Rep (2017) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; Rhesus monkey; loading ...; fig 3b
In order to establish the ability of the herpes simplex virus-1 VC2 vaccine to elicit immune responses in rhesus macaques, BD Biosciences CD21 antibody (BD Biosciences, Bly4) was used in flow cytometry on Rhesus monkey samples (fig 3b). Vaccine (2017) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; Rhesus monkey; loading ...; fig s7c
In order to assess the efficacy of targeting alpha4 beta7 integrin to treat SIV, BD Biosciences CD21 antibody (BD Pharmingen, 551064) was used in flow cytometry on Rhesus monkey samples (fig s7c). Science (2016) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human; loading ...; fig st1
BD Biosciences CD21 antibody (BD Biosciences, 555422) was used in flow cytometry on human samples (fig st1). PLoS ONE (2016) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human; loading ...; fig 1a
In order to investigate how differences in B cell populations may contribute to HIV susceptibility, BD Biosciences CD21 antibody (BD, B-ly4) was used in flow cytometry on human samples (fig 1a). AIDS Res Hum Retroviruses (2016) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human; loading ...; fig 2b
In order to characterize B cells and antibody repertoires from injection drug users, BD Biosciences CD21 antibody (BD, B-ly4) was used in flow cytometry on human samples (fig 2b). PLoS ONE (2016) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; Rhesus monkey; 1:85
In order to optimize vaccination with Aventis Pasteur's canarypox vector-HIV, BD Biosciences CD21 antibody (BD Biosciences, 561374) was used in flow cytometry on Rhesus monkey samples at 1:85. Nat Med (2016) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences CD21 antibody (BD, 555422) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human; loading ...; fig 3a
In order to characterize B and T cells from patients with thrombocytopenia, BD Biosciences CD21 antibody (BD Biosciences, 555422) was used in flow cytometry on human samples (fig 3a). Haematologica (2016) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human
In order to assess the role of B cells in tolerant kidney transplant recipients, BD Biosciences CD21 antibody (BD, B-ly4) was used in flow cytometry on human samples . Am J Transplant (2015) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human
In order to discuss the use of stem cells to treat Wiskott-Aldrich syndrome, BD Biosciences CD21 antibody (BD, B-ly4) was used in flow cytometry on human samples . J Allergy Clin Immunol (2015) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human
BD Biosciences CD21 antibody (BD Biosciences, B-ly4) was used in flow cytometry on human samples . J Immunol (2015) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human
BD Biosciences CD21 antibody (BD, B-ly4) was used in flow cytometry on human samples . Nat Commun (2014) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human; loading ...; fig 1a
BD Biosciences CD21 antibody (Becton Dickinson, B-ly4) was used in flow cytometry on human samples (fig 1a). Clin Immunol (2014) ncbi
mouse monoclonal (B-ly4)
  • flow cytometry; human
BD Biosciences CD21 antibody (BD Biosciences, B-ly4) was used in flow cytometry on human samples . J Allergy Clin Immunol (2014) ncbi
Articles Reviewed
  1. Zumaquero E, Stone S, Scharer C, Jenks S, Nellore A, Mousseau B, et al. IFNγ induces epigenetic programming of human T-bethi B cells and promotes TLR7/8 and IL-21 induced differentiation. elife. 2019;8: pubmed publisher
  2. Bouafia A, Lofek S, Bruneau J, Chentout L, Lamrini H, Trinquand A, et al. Loss of ARHGEF1 causes a human primary antibody deficiency. J Clin Invest. 2019;129:1047-1060 pubmed publisher
  3. Burton A, Pallett L, McCoy L, Suveizdyte K, Amin O, Swadling L, et al. Circulating and intrahepatic antiviral B cells are defective in hepatitis B. J Clin Invest. 2018;128:4588-4603 pubmed publisher
  4. Jegaskanda S, Mason R, Andrews S, Wheatley A, Zhang R, Reynoso G, et al. Intranasal Live Influenza Vaccine Priming Elicits Localized B Cell Responses in Mediastinal Lymph Nodes. J Virol. 2018;92: pubmed publisher
  5. Garcia Hernandez M, Uribe Uribe N, Espinosa González R, Kast W, Khader S, Rangel Moreno J. A Unique Cellular and Molecular Microenvironment Is Present in Tertiary Lymphoid Organs of Patients with Spontaneous Prostate Cancer Regression. Front Immunol. 2017;8:563 pubmed publisher
  6. Torii Y, Kawada J, Murata T, Yoshiyama H, Kimura H, Ito Y. Epstein-Barr virus infection-induced inflammasome activation in human monocytes. PLoS ONE. 2017;12:e0175053 pubmed publisher
  7. Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  8. Wentink M, Dalm V, Lankester A, van Schouwenburg P, Schölvinck L, Kalina T, et al. Genetic defects in PI3K? affect B-cell differentiation and maturation leading to hypogammaglobulineamia and recurrent infections. Clin Immunol. 2017;176:77-86 pubmed publisher
  9. Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren H, Sonnerborg A, et al. Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals. Sci Rep. 2017;7:40354 pubmed publisher
  10. Stanfield B, Pahar B, Chouljenko V, Veazey R, Kousoulas K. Vaccination of rhesus macaques with the live-attenuated HSV-1 vaccine VC2 stimulates the proliferation of mucosal T cells and germinal center responses resulting in sustained production of highly neutralizing antibodies. Vaccine. 2017;35:536-543 pubmed publisher
  11. Izawa K, Martin E, Soudais C, Bruneau J, Boutboul D, Rodriguez R, et al. Inherited CD70 deficiency in humans reveals a critical role for the CD70-CD27 pathway in immunity to Epstein-Barr virus infection. J Exp Med. 2017;214:73-89 pubmed publisher
  12. Fromm J, Thomas A, Wood B. Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting. Am J Pathol. 2017;187:304-317 pubmed publisher
  13. Byrareddy S, Arthos J, Cicala C, Villinger F, Ortiz K, Little D, et al. Sustained virologic control in SIV+ macaques after antiretroviral and α4β7 antibody therapy. Science. 2016;354:197-202 pubmed
  14. Clavarino G, Delouche N, Vettier C, Laurin D, Pernollet M, Raskovalova T, et al. Novel Strategy for Phenotypic Characterization of Human B Lymphocytes from Precursors to Effector Cells by Flow Cytometry. PLoS ONE. 2016;11:e0162209 pubmed publisher
  15. Keefer M, Zheng B, Rosenberg A, Kobie J. Increased Steady-State Memory B Cell Subsets Among High-Risk Participants in an HIV Vaccine Trial. AIDS Res Hum Retroviruses. 2016;32:1143-1148 pubmed
  16. Zenarruzabeitia O, Vitallé J, Garcia Obregon S, Astigarraga I, Eguizabal C, Santos S, et al. The expression and function of human CD300 receptors on blood circulating mononuclear cells are distinct in neonates and adults. Sci Rep. 2016;6:32693 pubmed publisher
  17. Ferre E, Rose S, Rosenzweig S, Burbelo P, Romito K, Niemela J, et al. Redefined clinical features and diagnostic criteria in autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy. JCI Insight. 2016;1: pubmed
  18. Agaimy A, Michal M, Hadravsky L, Michal M. Follicular dendritic cell sarcoma: clinicopathologic study of 15 cases with emphasis on novel expression of MDM2, somatostatin receptor 2A, and PD-L1. Ann Diagn Pathol. 2016;23:21-8 pubmed publisher
  19. Piepenbrink M, Samuel M, Zheng B, Carter B, Fucile C, Bunce C, et al. Humoral Dysregulation Associated with Increased Systemic Inflammation among Injection Heroin Users. PLoS ONE. 2016;11:e0158641 pubmed publisher
  20. Vaccari M, Gordon S, Fourati S, Schifanella L, Liyanage N, Cameron M, et al. Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition. Nat Med. 2016;22:762-70 pubmed publisher
  21. Shi H, Cao N, Pu Y, Xie L, Zheng L, Yu C. Long non-coding RNA expression profile in minor salivary gland of primary Sjögren's syndrome. Arthritis Res Ther. 2016;18:109 pubmed publisher
  22. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  23. Flint S, Gibson A, Lucas G, Nandigam R, Taylor L, Provan D, et al. A distinct plasmablast and naïve B-cell phenotype in primary immune thrombocytopenia. Haematologica. 2016;101:698-706 pubmed publisher
  24. Newell K, Asare A, Sanz I, Wei C, Rosenberg A, Gao Z, et al. Longitudinal studies of a B cell-derived signature of tolerance in renal transplant recipients. Am J Transplant. 2015;15:2908-20 pubmed publisher
  25. Berg A, Soma L, Clark B, Swerdlow S, Roth C. Evaluating breast lymphoplasmacytic infiltrates: a multiparameter immunohistochemical study, including assessment of IgG4. Hum Pathol. 2015;46:1162-70 pubmed publisher
  26. Figenschau S, Fismen S, Fenton K, Fenton C, Mortensen E. Tertiary lymphoid structures are associated with higher tumor grade in primary operable breast cancer patients. BMC Cancer. 2015;15:101 pubmed publisher
  27. Tandon B, Swerdlow S, Hasserjian R, Surti U, Gibson S. Chronic lymphocytic leukemia/small lymphocytic lymphoma: another neoplasm related to the B-cell follicle?. Leuk Lymphoma. 2015;56:3378-86 pubmed publisher
  28. Rajnai H, Teleki I, Kiszner G, Meggyesházi N, Balla P, Vancsik T, et al. Connexin 43 communication channels in follicular dendritic cell development and in follicular lymphomas. J Immunol Res. 2015;2015:528098 pubmed publisher
  29. Castiello M, Scaramuzza S, Pala F, Ferrua F, Uva P, Brigida I, et al. B-cell reconstitution after lentiviral vector-mediated gene therapy in patients with Wiskott-Aldrich syndrome. J Allergy Clin Immunol. 2015;136:692-702.e2 pubmed publisher
  30. Zinöcker S, Schindler C, Skinner J, Rogosch T, Waisberg M, Schickel J, et al. The V gene repertoires of classical and atypical memory B cells in malaria-susceptible West African children. J Immunol. 2015;194:929-39 pubmed publisher
  31. Chovancová J, Bernard T, Stehlíková O, Sálek D, Janíková A, Mayer J, et al. Detection of Minimal Residual Disease in Mantle Cell Lymphoma. Establishment of Novel 8-Color Flow Cytometry Approach. Cytometry B Clin Cytom. 2014;: pubmed publisher
  32. Willmann K, Klaver S, DoÄŸu F, Santos Valente E, Garncarz W, Bilic I, et al. Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity. Nat Commun. 2014;5:5360 pubmed publisher
  33. Jovanovic D, Djurdjevic P, Andjelkovic N, Zivić L. Possible role of CD22, CD79b and CD20 expression in distinguishing small lymphocytic lymphoma from chronic lymphocytic leukemia. Contemp Oncol (Pozn). 2014;18:29-33 pubmed publisher
  34. Gomez Choco M, Doucerain C, Urra X, Planas A, Chamorro A. Presence of heat shock protein 70 in secondary lymphoid tissue correlates with stroke prognosis. J Neuroimmunol. 2014;270:67-74 pubmed publisher
  35. Mueller A, Brieske C, Schinke S, Csernok E, Gross W, Hasselbacher K, et al. Plasma cells within granulomatous inflammation display signs pointing to autoreactivity and destruction in granulomatosis with polyangiitis. Arthritis Res Ther. 2014;16:R55 pubmed publisher
  36. Doi H, Tanoue S, Kaplan D. Peripheral CD27-CD21- B-cells represent an exhausted lymphocyte population in hepatitis C cirrhosis. Clin Immunol. 2014;150:184-91 pubmed publisher
  37. Lighaam L, Vermeulen E, Bleker T, Meijlink K, Aalberse R, Barnes E, et al. Phenotypic differences between IgG4+ and IgG1+ B cells point to distinct regulation of the IgG4 response. J Allergy Clin Immunol. 2014;133:267-70.e1-6 pubmed publisher
  38. Lorenzi L, Lonardi S, Petrilli G, Tanda F, Bella M, Laurino L, et al. Folliculocentric B-cell-rich follicular dendritic cells sarcoma: a hitherto unreported morphological variant mimicking lymphoproliferative disorders. Hum Pathol. 2012;43:209-15 pubmed publisher
  39. Petrilli G, Lorenzi L, Paracchini R, Ubiali A, Schumacher R, Cabassa P, et al. Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature. Int J Surg Pathol. 2014;22:712-21 pubmed publisher
  40. Contamin H, Loizon S, Bourreau E, Michel J, Garraud O, Mercereau Puijalon O, et al. Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey). J Immunol Methods. 2005;297:61-71 pubmed