This is a Validated Antibody Database (VAD) review about human CD100, based on 6 published articles (read how Labome selects the articles), using CD100 antibody in all methods. It is aimed to help Labome visitors find the most suited CD100 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CD100 synonym: C9orf164; CD100; COLL4; M-sema-G; SEMAJ; coll-4; semaphorin-4D; BB18; GR3; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D

R&D Systems
mouse monoclonal (758734)
  • blocking or activating experiments; human; loading ...; fig 2a
In order to study the interaction between CD100 and CD72 and its role in T cell proliferation, R&D Systems CD100 antibody (R&D Systems, 758734) was used in blocking or activating experiments on human samples (fig 2a). Front Immunol (2017) ncbi
mouse monoclonal (758726)
  • blocking or activating experiments; human; loading ...; fig 2a
In order to study the interaction between CD100 and CD72 and its role in T cell proliferation, R&D Systems CD100 antibody (R&D Systems, 758726) was used in blocking or activating experiments on human samples (fig 2a). Front Immunol (2017) ncbi
BioLegend
mouse monoclonal (A8)
  • flow cytometry; human; 1:50; loading ...; fig s3
In order to study the interaction between CD100 and CD72 and its role in T cell proliferation, BioLegend CD100 antibody (BioLegend, A8) was used in flow cytometry on human samples at 1:50 (fig s3). Front Immunol (2017) ncbi
mouse monoclonal (A8)
  • flow cytometry; human; loading ...; fig st12
In order to identify new types of human blood dendritic cells, monocytes, and progenitors through single-cell RNA-seq, BioLegend CD100 antibody (Biolegend, A8) was used in flow cytometry on human samples (fig st12). Science (2017) ncbi
Invitrogen
mouse monoclonal (eBio133-1C6 (133-1C6))
  • blocking or activating experiments; human; loading ...; fig 2a
In order to study the interaction between CD100 and CD72 and its role in T cell proliferation, Invitrogen CD100 antibody (eBiosciences, eBio133-1C6) was used in blocking or activating experiments on human samples (fig 2a). Front Immunol (2017) ncbi
mouse monoclonal (eBio133-1C6 (133-1C6))
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, Invitrogen CD100 antibody (e- Bioscienc e, 53-1009) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
Miltenyi Biotec
human recombinant (REA316)
  • flow cytometry; human; loading ...; fig st12
In order to identify new types of human blood dendritic cells, monocytes, and progenitors through single-cell RNA-seq, Miltenyi Biotec CD100 antibody (Miltenyi Biotec, REA316) was used in flow cytometry on human samples (fig st12). Science (2017) ncbi
BD Biosciences
mouse monoclonal (30/CD100)
  • blocking or activating experiments; human; loading ...; fig 2a
In order to study the interaction between CD100 and CD72 and its role in T cell proliferation, BD Biosciences CD100 antibody (BD, 30/CD100) was used in blocking or activating experiments on human samples (fig 2a). Front Immunol (2017) ncbi
mouse monoclonal (30/CD100)
  • immunohistochemistry - paraffin section; human; 1:100; fig 1
  • ELISA; human; fig 2
  • western blot; human; fig 3
BD Biosciences CD100 antibody (BD Biosciences, 610670) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1), in ELISA on human samples (fig 2) and in western blot on human samples (fig 3). Onco Targets Ther (2016) ncbi
mouse monoclonal (30/CD100)
  • blocking or activating experiments; human; 10 ug/ml; fig 5d
  • western blot; human; fig 2a
In order to study the role of human head and neck squamous cell carcinoma-associated semaphorin 4d in myeloid-derived suppressor cells, BD Biosciences CD100 antibody (BD Biosciences, 610670) was used in blocking or activating experiments on human samples at 10 ug/ml (fig 5d) and in western blot on human samples (fig 2a). J Immunol (2016) ncbi
mouse monoclonal (30/CD100)
  • western blot; human
BD Biosciences CD100 antibody (BD Biosciences, 30/CD100) was used in western blot on human samples . Blood (2013) ncbi
Sigma-Aldrich
mouse monoclonal (3B4)
  • blocking or activating experiments; human; loading ...; fig 2a
In order to study the interaction between CD100 and CD72 and its role in T cell proliferation, Sigma-Aldrich CD100 antibody (Sigma-Aldrich, 3B4) was used in blocking or activating experiments on human samples (fig 2a). Front Immunol (2017) ncbi
Articles Reviewed
  1. Jiang X, Björkström N, Melum E. Intact CD100-CD72 Interaction Necessary for TCR-Induced T Cell Proliferation. Front Immunol. 2017;8:765 pubmed publisher
  2. Villani A, Satija R, Reynolds G, Sarkizova S, Shekhar K, Fletcher J, et al. Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. Science. 2017;356: pubmed publisher
  3. Ding X, Qiu L, Zhang L, Xi J, Li D, Huang X, et al. The role of semaphorin 4D as a potential biomarker for antiangiogenic therapy in colorectal cancer. Onco Targets Ther. 2016;9:1189-204 pubmed publisher
  4. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  5. Younis R, Han K, Webb T. Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells. J Immunol. 2016;196:1419-29 pubmed publisher
  6. Mou P, Zeng Z, Li Q, Liu X, Xin X, WANNEMACHER K, et al. Identification of a calmodulin-binding domain in Sema4D that regulates its exodomain shedding in platelets. Blood. 2013;121:4221-30 pubmed publisher