This is a Validated Antibody Database (VAD) review about human CBX5, based on 15 published articles (read how Labome selects the articles), using CBX5 antibody in all methods. It is aimed to help Labome visitors find the most suited CBX5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CBX5 synonym: HEL25; HP1; HP1A

Knockout validation
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig ev5a
Cell Signaling Technology CBX5 antibody (Cell Signaling Technology., 2616) was used in western blot knockout validation on human samples at 1:1000 (fig ev5a). EMBO J (2018) ncbi
Abcam
domestic rabbit monoclonal (EPR5777)
  • immunohistochemistry - frozen section; human; loading ...; fig 1b, 1c
Abcam CBX5 antibody (Abcam, ab109028) was used in immunohistochemistry - frozen section on human samples (fig 1b, 1c). Science (2020) ncbi
domestic rabbit monoclonal (EPR5777)
  • western blot; human; loading ...; fig s7b
Abcam CBX5 antibody (Abcam, ab109028) was used in western blot on human samples (fig s7b). Cancer Cell (2019) ncbi
domestic rabbit monoclonal
  • immunocytochemistry; mouse; 1:500; loading ...; fig 3a
Abcam CBX5 antibody (Abcam, ab203432) was used in immunocytochemistry on mouse samples at 1:500 (fig 3a). Nature (2019) ncbi
domestic goat polyclonal
  • immunocytochemistry; mouse; loading ...; fig s8a
Abcam CBX5 antibody (Abcam, ab77256) was used in immunocytochemistry on mouse samples (fig s8a). Sci Rep (2018) ncbi
domestic rabbit monoclonal (EPR5777)
  • western blot; mouse; loading ...; fig 1k
In order to study the role of beta actin in chromatin organization and cellular identity, Abcam CBX5 antibody (Abcam, ab109028) was used in western blot on mouse samples (fig 1k). FASEB J (2018) ncbi
Invitrogen
mouse recombinant
  • immunoprecipitation; human; 2 ug
In order to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality, Invitrogen CBX5 antibody (life tech, 730046) was used in immunoprecipitation on human samples at 2 ug. Nat Methods (2015) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; African green monkey; 1:400; loading ...; fig 2d
Cell Signaling Technology CBX5 antibody (Cell Signaling, 2616s) was used in immunohistochemistry - paraffin section on African green monkey samples at 1:400 (fig 2d). Protein Cell (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 4g
  • western blot; human; loading ...; fig 2c, 2h
Cell Signaling Technology CBX5 antibody (Cell Signaling, 2616S) was used in immunocytochemistry on human samples (fig 4g) and in western blot on human samples (fig 2c, 2h). Nucleic Acids Res (2020) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 5i
Cell Signaling Technology CBX5 antibody (Cell Signaling, 2616) was used in immunocytochemistry on human samples (fig 5i). elife (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 3a
Cell Signaling Technology CBX5 antibody (Cell Signaling, 2616) was used in western blot on mouse samples (fig 3a). Cell Signal (2018) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig ev5a
Cell Signaling Technology CBX5 antibody (Cell Signaling Technology., 2616) was used in western blot knockout validation on human samples at 1:1000 (fig ev5a). EMBO J (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 6c
Cell Signaling Technology CBX5 antibody (Cell Signaling, 2616S) was used in western blot on human samples at 1:500 (fig 6c). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 3
Cell Signaling Technology CBX5 antibody (Cell Signaling, 2616) was used in western blot on human samples (fig 3). Protein Cell (2016) ncbi
domestic rabbit monoclonal (C7F11)
  • immunocytochemistry; human; 1:100; fig 3b
  • western blot; human; 1:1000; fig 3a
Cell Signaling Technology CBX5 antibody (Cell Signaling Technology, 2623) was used in immunocytochemistry on human samples at 1:100 (fig 3b) and in western blot on human samples at 1:1000 (fig 3a). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 5g
Cell Signaling Technology CBX5 antibody (Cell Signaling, 2616) was used in western blot on human samples (fig 5g). Aging Cell (2016) ncbi
Articles Reviewed
  1. Zhang H, Li J, Ren J, Sun S, Ma S, Zhang W, et al. Single-nucleus transcriptomic landscape of primate hippocampal aging. Protein Cell. 2021;12:695-716 pubmed publisher
  2. Klein I, Boija A, Afeyan L, Hawken S, Fan M, Dall Agnese A, et al. Partitioning of cancer therapeutics in nuclear condensates. Science. 2020;368:1386-1392 pubmed publisher
  3. Hu H, Ji Q, Song M, Ren J, Liu Z, Wang Z, et al. ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin. Nucleic Acids Res. 2020;48:6001-6018 pubmed publisher
  4. Alessio N, Squillaro T, Di Bernardo G, Galano G, De Rosa R, Melone M, et al. Increase of circulating IGFBP-4 following genotoxic stress and its implication for senescence. elife. 2020;9: pubmed publisher
  5. Gomes A, Ilter D, Low V, Rosenzweig A, Shen Z, Schild T, et al. Dynamic Incorporation of Histone H3 Variants into Chromatin Is Essential for Acquisition of Aggressive Traits and Metastatic Colonization. Cancer Cell. 2019;36:402-417.e13 pubmed publisher
  6. Guo Y, Manteiga J, Henninger J, Sabari B, Dall Agnese A, Hannett N, et al. Pol II phosphorylation regulates a switch between transcriptional and splicing condensates. Nature. 2019;572:543-548 pubmed publisher
  7. Hervás Corpión I, Guiretti D, Alcaraz Iborra M, Olivares R, Campos Caro A, Barco A, et al. Early alteration of epigenetic-related transcription in Huntington's disease mouse models. Sci Rep. 2018;8:9925 pubmed publisher
  8. Lee C, Hsieh T. Wuho/WDR4 deficiency inhibits cell proliferation and induces apoptosis via DNA damage in mouse embryonic fibroblasts. Cell Signal. 2018;47:16-26 pubmed publisher
  9. Ruppert J, Samejima K, Platani M, Molina O, Kimura H, Jeyaprakash A, et al. HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry. EMBO J. 2018;37: pubmed publisher
  10. Xie X, Almuzzaini B, Drou N, Kremb S, Yousif A, Farrants A, et al. β-Actin-dependent global chromatin organization and gene expression programs control cellular identity. FASEB J. 2018;32:1296-1314 pubmed publisher
  11. Zhang H, Sun L, Wang K, Wu D, Trappio M, Witting C, et al. Loss of H3K9me3 Correlates with ATM Activation and Histone H2AX Phosphorylation Deficiencies in Hutchinson-Gilford Progeria Syndrome. PLoS ONE. 2016;11:e0167454 pubmed publisher
  12. Li Y, Zhang W, Chang L, Han Y, Sun L, Gong X, et al. Vitamin C alleviates aging defects in a stem cell model for Werner syndrome. Protein Cell. 2016;7:478-88 pubmed publisher
  13. Huang R, Langdon S, Tse M, Mullen P, Um I, Faratian D, et al. The role of HDAC2 in chromatin remodelling and response to chemotherapy in ovarian cancer. Oncotarget. 2016;7:4695-711 pubmed publisher
  14. Xiong Z, Choi J, Wang K, Zhang H, Tariq Z, Wu D, et al. Methylene blue alleviates nuclear and mitochondrial abnormalities in progeria. Aging Cell. 2016;15:279-90 pubmed publisher
  15. Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, et al. Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation. Nat Methods. 2015;12:725-31 pubmed publisher